Objective To study the relationship between the expression of p16 protein and the biological behavour of thyroid tumor. Methods The expression of p16 protein was examined in fifty cases of thyroid tumor by immunohistochemical staining technique(SABC method). The relationship between p16 protein expression and tumor size, pathological type, differention grading and clinical stage of thyroid tumors were analysed. Results In thyroid adenoma, p16 protein expression was detected in 13 out of 15 cases(86.7%); in thyroid carcinoma p16 protein was expressed only in 16 out of 35 cases(45.7%), the difference was significant(P

Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

AIM:To improve the standard of quality control of Yiqi Zhixue Granule(Radix Codonopsis,Radix Astragali,Rhizoma Atractylodis macrocephalae,etc.) METHODS: In the prescription,both Radix codonopsis and Radix Astragali were identified by HPLC.Folium mahoniae was distinguished by TLC.Rhizoma Bletillae was authenticated by microscope.Morever,RP-HPLC method was adopted to determine the astragaloside content in the productions,using a column of C_(18),the column temperature was at 35(?C),acetonitrile-water(32∶68) as a mobile phase,he flow rate was 1.0 mL/min,and ELSD as detection in which the tube temperature was set at 102(?C) and the air flow was at 2.8 mL/min,the impactor was chosen off. RESULTS: The established identification methods were simple and proper,reflecting a good specificity,while the determination method was accurate and reliable,which showed the recovery of 97.95%,RSD=2.21%(n=9),the intermediate precision of RSD=2.67%(n=3),the robustness of RSD=3.67%(n=3) between columns,and the LOQ of 0.539 2 ?g,RSD=2.92%(n=5). CONCLUSION: The improved standard of quality control of Yiqi Zhixue Granule is not only practical but capable of effectively controlling the quality of the medicine as well.

Objective To study the clinical significance of interleukin (IL)-6 and IL-13 levels in serum of hand,foot,and mouth disease (HFMD)caused by enterovirus 71,and carries on the preliminary discussion on serum IL-6 and IL-13 cell origin. Methods Serum levels of IL-6 and IL-13 in HFMD and healthy control were detected by enzyme linked immunosorbent assay (ELISA),serum IL-6 and IL-13 levels among different severity of HFMD were compared.Serum IL-6 and IL-13 levels in HFMD after treatment were also detected.The secretion of IL-6 by monocyte and the secretion of IL-13 by T cells in peripheral blood mononuclear cells (PBMC)were detected by Flow Cytometry (FCM).Monocyte and T cells from health individual were treated with EV71,and the secretion of IL-6 by monocyte and the secretion of IL-13 by T cells were detected by FCM too.Results Serum levels of IL-6 and IL-13 were (79.63±29.45)pg/mL and (36.45±1 5.13)pg/mL respectively in HFMD,and serum levels of IL-6 and IL-13 were (27.26±7.82)pg/mL and (13.46±3.14)pg/mL respectively in health control,serum IL-6 and IL-13 levels in HFMD were significantly higher than that of healthy subjects (P < 0.01 ),and the mean serum IL-6 and IL-13 levels of HFMD were increased with the severity of the disease (P <0.01).After treatment,serum levels of IL-6 and IL-13 were (48.23 ±23.14) pg/mL and (23.25±9.63)pg/mL respectively,when compared with before treatment,serum levels of IL-6 and IL-13 were signifi-cantly decreased (P <0.01).The secretion ability of IL-6 by monocyte and the secretion of IL-13 by T cells in HFMD were signifi-cantly higher than that of healthy subjects.After infection by EV71,the secretion ability of IL-6 by monocyte and the secretion of IL-13 by T cells were significantly increased.Conclusion Infection of EV71 probably by increase the secretion ability of IL-6 by monocyte and the secretion of IL-13 by T cells,resulted in elevated of serum IL-6 and IL-13 levels,which involved in the occurrence and development of HFMD.

Saxagliptin belongs to the class of dipeptidyl peptidase-4(DPP-4) inhibitors which are novel oral glucose-lowering agents with uniquely mechanism in patients with type 2 diabetes mellitus. Clinical efficacy without increasing hypoglycemia of saxagliptin as single agent as well as in combination with other medications used for the treatment of type 2 diabetes mellitus has been well established in several randomized trials. In addition, a cardiovascular outcome study of SAVOR shows that saxagliptin does not increase the risk of a composite of non-fatal myocardial infarction, non-fatal stroke or cardiovascular death meeting the primary safety objective. The efficacy and safety studies were hereby reviewed.

In this paper , the author analyzed the network media with three main characteristics of negative re-ports about doctor -patient dispute , from four aspects explains the network media in building a harmonious doctor-patient relationship in ethics responsibility: public opinion guide , social responsibility , self-discipline to bear the ethical responsibility and abide by relevant laws and regulations , accept the heteronomy , points out that the net-work media should strengthen self -discipline and self -reflection are scientific and objective , guide the netizens rational appeal , to assume the social ethical responsibility of building a harmonious doctor -patient relationship .

〔Abstract〕 By retrieving the literatures from the official website of ONC , Tongfang Knowledge Network and the Internet , the paper conducts a survey of the conformance test and certification of health information standards in America from certification subject , certifica-tion object, test method and process, certification standard, certification mode and process, etc.It aims to provide a reference for the conformance test of health information standard in China .

Objective:Mobile X-ray radiographic Units to appear in the clinical application of high voltage circuit judge special case of failure, the analysis in order to provide effective and reliable service basis. Methods: Preliminary judgment based fault location fault code, using stepwise exclusion analyze, judge, and determine the cause of the failure lies. Results:The high-voltage inverter element models of mobile DR, the driver circuit, the output voltage discharge waveform judgment and other issues have a better understanding, to facilitate the exclusion of equipment failure. Conclusion:Special case of X-ray radiographic Units failure high voltage circuit analysis and troubleshooting, especially for the inverter circuit analysis, X-ray radiographic appear to take timely and appropriate repair method when high pressure overload.

Etiological and serological testing, including virus genome, virus antigen, anti-virus antibodies, virus culture and identification, were the standard laboratory tests to confirm or exclude Ebola virus infections.Moreover, other laboratory tests, such as blood routine tests, liver and kidney function tests, serum electrolyte tests, and coagulation function tests, were very important to the clinical diagnosis and treatment of Ebola virus disease ( EVD) patients.Based on working experiences and practices in the China Ebola Treatment Center and available publications, opinions about the detailed clinical significance and associated key point of biosafty prevention and protection protocols of above described laboratory tests for the clinical diagnosis and treatment of EVD were provided.

Objective To investigate the risk factors and clinical features of severe preeclampsia(SP) complicated with multiple organ dysfunction syndrome (Multiple Organ Dysfunction Syndrome,MODS) in order to provide reference for clinical practice.Methods Three hundred and ninety-one cases with severe preeclampsia were selected as our subjects,who hospitalized from February 2005 to February 2014 in our hospital.All subjects were divided into observation group (48 cases with MODS) and control group (343 cases without MODS).Clinical data were collected including age,gravidity,parity,gestational age,gestational age termination of pregnancy,uterine scar,prenatal care,medical history,treatment,mother child outcomes.Results There were significant differences between two groups in terms of weeks of termination of pregnancy,rate of uterine scar,rate of regular check-up of pregnancy,rate of chronic hypertension and renal disease.Logistic results showed that there were relationships between SP complicated with MO)S and the occurrence of termination of pregnancy with gestational age ＜28 weeks (OR =2.046,95％ CI:1.03 ～ 3.74,P =0.034),uterine scar (OR =2.401,95％ CI:1.16 ～ 4.09,P =0.008),non-regular prenatal care (OR =3.167,95％ CI:1.96 ～ 5.23,P =0.002),chronic hypertension (OR =3.348,95％ CI:2.23 ～ 5.78,P =0.001),renal disease (OR =3.064,95％ CI:1.77 ～ 5.18,P =0.038).The rate of underwent special treatment,preterm children,neonatal asphyxia,perinatal death,intracranial hemorrhage in observation group were 77.08％,79.17％,29.17％,20.83％,4.17％,higher than that in control group (23.62％,45.19％,13.70％,8.45％,1.17％ respectively; x2 =10.471,12.147,4.519,8.644,6.648,all P ＜ 0.05).The degree of organ injury score in gestational age ≥ 33 weeks and ＜ 33 weeks were (1.34 ± 0.28) points and (3.64 ± 0.69) points,and the difference was significant.The gestational week in gestational age ≥ 33 weeks and ＜33 weeks were (35.48 ±2.06) weeks and (29.87 ± 1.37) weeks,and the differencewas significant(t =3.917,P ＜0.05).The survival rates were 100％,77.78％,33.33％ when the numbers of damaged organs were 2,3 and ≥ 4.There was significant different between 2 and 3 damaged organs in terms of survival rate (x2 =3.964,P =0.024).Conclusion The risk factors of severe preeclampsia complicated with MODS include termination of pregnancy gestational age ＜ 28 weeks,uterine scar,non-regular prenatal care,chronic hypertension,renal disease.Prognosis of severe preeclampsia patients with MODs was poor than that without MODS and it relates with gestational age,the number of closely related organ damage.