AIM: To establish the quality standard for Compound Songluo Granule (Rhizoma Corydalis, Radix Angelicae Sinensis, Lichen Usneae). METHODS:Rhizoma Corydalis, Radix Angelicae Sinensis were identified by TLC. Usnic acid in Lichen Usneae was determined by TLCS. RESULTS: Rhizoma Corydalis, Radix Angelicae Sinensis could be detected by TLC. Usnic acid showed a good linear relationship at the range of 0. 50～2.50 ?g, r=~0.999 9. The average recovery was 97.5% and RSD was 2.61%, respectively. CONCLUSION:The method is available with a good reproducibility and can be used for the quality control of Compound Songluo Granule.

Objective To study the clinical characteristics and imaging features of alien hand syndrome (AHS). Methods We retrospectively analyzed the clinical data of 3 typical cases in our hospital,in comparison with case reports and reviews of domestic and foreign during the past decade. Result All 3 patients showed corpus callosum lesions from CT or MRI detections. The symptoms of the patients included alien hand sign , abnormal involuntary movements, intermanual conflict of upper limbs, and so on. And the patients accompanied with emotional and cognitive changes. Two of them were improved in different degrees after treatment. Conclusion AHS was a complex disease. The diagnosis of this syndrome was based on the advanced neuroimaging. There was still no well established treatment for AHS.

Angioplasty, Balloon, Coronary ; Coronary Restenosis ; prevention & control ; Endothelial Cells ; cytology ; Endothelium ; cytology ; Humans ; Stem Cells ; cytology ; Stents

Thyroid-stimulating hormone receptor(TSHR)is an important autoantigen whose cleavage and shedding can induce autoimmunity.The TSHR is the main functional receptor of the thyroid.The shedding of the ? subunit plays an important role in the forming of TSAb and balancing between TSAb and TBAb.Research on the cleavage and shedding of the TSHR extracellualar domain helps to gain a deep insight into the structure and function of TSHR,illuminate the pathogenesis of autoimmune thyroid disease and find out a new therapy.The structure of TSHR,the mechanism and the significance of its cleavage and shedding are reviewed in this article.

Objective To optimize the method and condition s for the extraction process of Fushuning Granules.Methods Different factors and levels were in vestigated by orthogonal design wit h yields of oleanolic acid and tanshi noneⅡ A as the markers.The yields of effective com ponents by percolation and reflux we re compared.Results The extraction ratio by re-flux was higher than that by percolation.The primary factors influencin g extraction ratio by reflux were the pulverization degree of medicinal materials,concentration of alcohol,refluxing times and refluxing perio d.Conclusion The opti-mum extraction process was:refluxing and extracting the pulverized med icinal materials for 3times(1hours each time )with 6-fold 90%alcohol added in the f irst time and then 5-fold in the secon d and the third time.

Objective To explore the effect of advanced glycation end products(AGEs) on the oxidative stress in porcine vascular endothelial cells(PIECs). Methods After being intervened by AGEs for some time,cell viability was detected by MTT.2′,7′-dichlorofluorescein diacetate(DCFH-DA) was used as a reactive oxygen species(ROS) capture agent.The fluorescent intensity of 2′,7′-dichlorofluorescein(DCF),which was the product of cellular oxidation of DCFH-DA,was detected by flow cytometry,and the level of ROS was thus measured. Results Viability of PIECs was inhibited by AGEs in a dose-and time-dependent fashion(P

Objective To investigate alterations in epigenetic regulation in B cells in subacute cutaneous lupus erythematosus (SCLE).Methods Peripheral blood mononuclear cells (PBMC) were isolated from the peripheral venous blood of SCLE patients and healthy donors with density gradient centrifugation.B cells were isolated with CD19 micro beads and protocols provided by the manufacturer.Total RNAs were isolated with RNA kits.The expressions of silence information regulator 1 (Sirt1) mRNA were investigated with quantitative real time polymerase chain reaction (PCR).Results The levels of Sirt1 mRNA were significantly increased in SCLE B cells relative to healthy controls (P ＜ 0.01).Conclusions Histone modifications appear abnormal in B cells in SCLE.

Child ; China ; Humans ; Neurology ; trends ; Pediatrics ; trends

Adult ; Female ; Humans ; Male ; Medical Staff, Hospital ; statistics & numerical data ; Middle Aged ; Nursing Staff, Hospital ; statistics & numerical data ; Sports ; statistics & numerical data

Objective To discuss clinical effects of combined use of duodenoscopy and laparoscopy in the treatment of acute billiary pancreatitis (ABP), and to establish a systematic protocol of minimally invasive treatment for ABP. Methods According to the patients’ biliary tract conditions, severity of disease and treatment methods, a total of 696 patients with ABP were given laparoscopic cholecystectomy (LC) alone, or endoscopic retrograde cholangiopancreatography (ERCP) and LC, or ERCP and laparoscopic common bile duct exploration (LCBDE), or ERCP and endoscopic sphincterotomy (EST), or individualized treatment for severe acute pancreatitis (SAP). Clinical effects were observed. Results Among the 696 patients, 330 patients (47.4%) received EST and biliary stones were successfully removed under endoscope in 267 patients (38.4%). ERCP, LC and LCBDE were conducted in 411 (59.1%), 513 (73.7%) and 85 (12.2%) patients, respectively, and successfully accomplished in 409 (99.5%), 511 (99.6%) and 82 (96.5%) patients, respectively. Out of 36 patients with SAP, 34 patients survived (94.4%). The total cure rate was 99.7% (694/696). Conclusions Combined use of duodenoscopy and laparoscopy is significantly effective for acute billiary pancreatitis and benefits the improvement and standardization of the protocol of minimally invasive treatment for acute billiary pancreatitis.