Objective To investigate the role of subdural puncture(SDP)in the diagnosis and treatment of subdural fluid collection in young children with purulent meningitis.Methods Totally 207

Cerebral Palsy ; diagnosis ; etiology ; therapy ; Humans ; Magnetic Resonance Imaging ; Prognosis

Humans ; Temporomandibular Joint Disorders ; therapy

Humans ; Quality of Life ; Rhinitis ; Sinusitis

Adolescent ; Asthma ; blood ; drug therapy ; Case-Control Studies ; Child ; Child, Preschool ; Female ; Humans ; Interleukin-17 ; blood ; Male

Adult ; Female ; Humans ; Male ; Middle Aged ; Parathyroid Neoplasms ; diagnostic imaging ; surgery ; Retrospective Studies ; Ultrasonography

Objective: To investigate the effects of immunosuppression treatment on peripheral nerve injury and regeneration in rats. Methods: Forty-nine SD rats were randomly divided into 7 groups: sciatic nerve (in the middle of left thigh) forceps-crushing+cyclophosphane, transecting+cyclophosphane, resecting groups+cyclophosphane, forceps-crushing+normal saline, transecting+normal saline, resecting groups+normal saline, and blank control groups. Cyclophosphane and normal saline were intraperitoneally injected into rats post-operatively. Peripheral nerve regeneration and its related function were assessed by walking track analysis, electrophysiology and histomorphology; immunohistochemistry method was used to evaluate the local autoimmune reactions 12 weeks after operation. Results: Cyclophosphane treated animals had higher scores of sciatic function index (SFI) compared to animals in the corresponding normal saline groups. The electrophysiological (nerve conduction velocity) and morphological examinations showed better regeneration of the myelinated axons in immunosuppression-treated animals compared to the corresponding normal saline groups. The immunohistochemistry showed that the intensities of the local immunological response in immunosuppression groups were obviously lower as compared to the corresponding normal saline groups. Conclusion: There is local autoimmune reaction in post-traumatic nerve regeneration and this autoimmune reaction may influence nerve regeneration. Cyclophosphane treatment can suppress this autoimmune reaction and improve the micro-environment for nerve regeneration.

AIM: To investigate the expression of Fas/FasL system in human lung carcinoma cell lines (A549, EBC-1, LCSC) and T-lymphocytes (Jurkat), and to search the possibilities of immune escape and counterattack mediated by Fas/FasL pathway in human lung carcinoma cells. METHODS: The protein and mRNA expression of Fas and FasL were detected by FACScan, RT-PCR, respectively. Cell apoptosis was assessed by fluorescent staining. Cell growth was determined by a trypan blue exclusion assay. RESULTS: Fas and FasL were expressed in 3 human lung carcinoma cell lines and T-cells, Jurkat. The lung carcinoma cells inhibited the growth (P

Objective To investigate the effects of Par-4 gene silence on hydrogen peroxide-induced apoptosis in alveolar epithelial cells. Method The alveolar epithelial cells A549 were cultured and exposed to hydrogen peroxide. The siRNA sequences targeted Par-4 gene was chemically synthesized and transfected to A549 cells with or without the exposure of hydrogen peroxide. The cells were divided into normal control groups, hydrogen peroxide-treated group(The cells were treated with 0. 1 mmol/L hydrogen peroxide), hydrogen peroxide and Par-4-siRNA-treated group(The cells were treated with 0. 1 mmol/L hydrogen peroxide after transfection of Par-4-siRNA), Non-specific DNA sequence transfection control group. The apoptosis of A549 cells was quantified by flow cytometry. The expression of Smac protein was detected by Western blot.Electrophoretic mobility shift assay was applied for evaluating the change of E2F1 DNA binding activity. Relative activity of Caspase-3 was detected by clolorimetric assay. Results The percent of apoptotic cells in hydrogen peroxide and Par-4-siRNA-treated group was (29.7 ± 2.3) %, which was significantly lower than that of hydrogen peroxide-treated group [(54.2 ± 4.1)%, q= 8.91, P ＜ 0.01)]. Par-4 siRNA could significantly suppress the increase of Smac protein, E2F1 DNA binding activity and caspase-3 activity induced by hydrogen peroxide in A549 cells. Conclusions Par-4 gene silence induced by siRNA might inhibit the apoptosis of alveolar epithelial cells, which might be resulted from suppression of the up-regulation of Smac gene expression, E2F1 DNA binding activity and caspase-3 activity.