Objective To study the appearance of skin mucous glycoprotein in vitro on the activity of human lung cancer cells A549. Methods Used alkali extraction and DEAE-52 ion exchange chromatography and Sephadex G-100 gel chromatography purification salamander mucous glycoprotein; Salamander mucous glycoprotein inhibition of human lung cancer cells A549 was detected by MTT colorimetric method in vitro.ResuIts It showed that the total sugar content in the appearance of mucus was 4.23%, the relatively pure glycoprotein component, by SDS protein electrophoresis tests, it contained a single glycoprotein component, its molecular weight was about 30 kDa.With glycoprotein pure concentration increased from 1,10, 20,40μg/mL, the glycoprotein inhibition rate of A549 cells increased; when the glycoprotein concentration was 40 μg/mL, for 24 h action, the inhibition rate of A549 cells was up to 85.66 %, while the role of 48 h, the inhibition rate of A549 cells was up to 92.32%.Inhibition effect of mucus glycoprotein on A549 cell compared with positive control drug, had significant difference.ConcIusion Salamander mucus glycoproteins of human lung cancer cells has obvious inhibitory effect, can provide theoretical basis for the development of lung cancer drug resistance.

Objective To explore whether the inflammatory stress can increase insulin resistance in the CD36 knockout (KO) mice.Methods After the mice were fed a standard chow for 14 weeks,oral glucose tolerance test,insulin release tests,lipids metabolism,SAA,IL-6,TNF-? and hepatic mRNA and proteins expression of mTOR,S6K,IRS-1,pIRS-1,2 were measured.Results Compared with the wide-type,the CD36 KO mice un-inflamed exhibited insulin resistance,and the insulin resistance index was increased[(3.01?1.24) vs (0.81?0.12),P

Hip Joint ; Humans ; Ischium/diagnostic imaging* ; Magnetic Resonance Imaging

Objective To analyze various state of anxiety of infertility women's access to an in vitro fertilization and embryo transfer (IVF-ET) after the treatment cycle, and the relattionship between personality trait and anxiety. Methods A total of 96 patients who received the IVF-ET treatment were surveyed by questionnaire for the day state-trait anxiety at the start of treatment cycle. The patients were investigated with the questionnaire at the day, before an hour ova harvest, waiting for ? human chorionic gonadotropin (β-HCG) at 13 days after post transplant respectively. Results (1) the mean of trait anxiety was 37.66±9.11. Three points in the survey had gradually increased (36.44±9.20, 39.37±11.27 and 40.98±12.73 respectively), the difference showed significant. (2) According to research group: a highly significant difference among the level of patients' anxiety was showed between those with high-trait anxiety value and low-trait anxiety value (P<0.05). Conclusions Nurses should pay close attention to psychological state of infertility women' s received IVF-ET and carry out measures to alleviate their psychological pressure so as to improve their treatment compliance and pregnancy success rate.

Aged ; Antigens, CD20 ; metabolism ; Humans ; Leukocyte Common Antigens ; metabolism ; Lymphoma, B-Cell ; complications ; metabolism ; pathology ; surgery ; Male ; Prostate ; pathology ; Prostatectomy ; Prostatic Hyperplasia ; complications ; metabolism ; pathology ; surgery

Acupuncture Therapy ; Chest Pain ; therapy ; Female ; Humans ; Middle Aged ; Stroke ; therapy

Objective To study the clinical pathological features, treatment and prognosis of umbilical metastases of colorectal carcinoma. Methods From January 2000 to September 2010, 10 umbilical metastases of colorectal carcinoma cases were admitted. The clinical features were reviewed. Results Four radical resection of colon cancer and resection of the umbilical plexus metastases cases with chemotherapeutic intraperitoneal perfusion lived 9,11,14 and 18 months respectively, 1 ileum-transverse colon anastomosis case with chemotherapeutic intraperitoneal perfusion lived six months, 2 patients with systemic widely transfer and umbilical transfer with pure venous chemotherapy lived 3, 3.5 months respectively, 3 colon intra-operative cases with venous chemotherapy lived 5 ,5. 5 and 7 months respectively. Conclusion Surgical resection of the primary focal and periumbilical metastases can prolong survival time with adjunctive therapy.

Objective To build the county level hospital nursing assistance need indicator system in Jiangxi province.Methods Literature review,qualitative research method and Delphi expert consultation method were adopted to build the county level hospital nursing assistance need indicator system.The indicator system was revised through trial test.Results Two rounds of expert consultation showed that expert authority degree coefficients were 0.79,0.83; Coordination coefficient(Kendall's W) was 0.11～0.16; content experts consistency coefficient was 0.78,reliability Cronbach's α coefficient was 0.85.Through expert advice and pre-examinaion,the county level hospital nursing assistance need indicator system was built,consisting of 3 level Ⅰ indicators,8 level Ⅱ indicators,43 level ⅠⅡ indicators,including assistance contents,assistance ways and assistance time.Conclusions This study established the county level hospital nursing assistance need indicator system with better scientific and feasible characteristics.It can provide experience and guidance for the relevance and effectiveness of the implementation of follow-up care aid work.

Objective To explore the enhancement patterns of pulmonary carcinomas by contrast-enhanced ultrasound(CEUS).Methods Thirty-eight patients with pulmonary carcinomas proven by pathology[28 with peripheral pulmonary carcinomas and 10 central pulmonary carcinoma with obstructive atelectasis(OA)]were examined by baseline ultrasound and contrast-enhanced ultrasound,then the arrival time(AT),time to peak(TTP)were analyzed with time-intensity curve analysis software and the dynamic enhancement pattern of each lesion was assessed.Results Twenty-four peripheral pulmonary carcinomas demonstrated delayed AT about 6-16 s after application of contrast medium,three lesions demonstrated early AT about 4-5 s and one lesion demonstrated absence of contrast enhancement.The lesions exhibited hyper-,hypo- and non-enhancement were 14,13 and 1,respectively.Seventeen lesions were heterogenous enhanced with non-enhanced necrosis areas and enhanced septa,while ten lesions homogeneous enhanced and one lesion no enhanced.Ten central pulmonary carcinoma with OA demonstrated a characteristic pattern:OA appeared a short AT(mean AT 4.8 s)until enhancement and strong contrast enhancement,while the central tumors appeared a delayed AT(mean AT 10.5 s)and faint enhancement.Conclusions CEUS can be useful in differentiation between solid and cystic pulmonary lesions,and detection of the latent lesions underlying the atelectasis.

Tetrabromobisphenol A(TBBPA)is supposed to have potential thyroid disrupting activities due to its similar structure to thyroid hormones.TBBPA has been proved to show thyroid disrupting effects in vivo and in vitro studies.TBBPA might disrupt thyroid hormone system through transthyretin- and thyroid receptor-mediated pathways.The ability of TBBPA inducing the production of reactive oxygen species might be the extension of its thyroid disrupting activities.The thyroid disrupting effects of TBBPA might be closely related to its oxidative stress,reproduction toxicity and neurotoxicity.More experiments are required for the effects of TBBPA on the aquatic and amphibian animals.