Urocortin,a novel peptide of the cortiotropin releasiong hormone(CRH)family,was discovered in 1995 in the rat brain and subsequently cloned and localized to human chromosome 2.It can bind to both CRH type 1 receptor and CRH type 2 receptor,as well as CRH binding protein.Especially to CRH type 2 receptor,urocortin binds to it with 40 times more potency than CRH does.Therefore,urocortin is thought to be an endogenous ligand for CRH type 2 receptor.Immunoreactive urocortin and urocortin mRNA were localized in many areas such as mammalian brain,cardiovascular system,placenta.It is believed that urocortinmay play important physiological roles as a neuropeptide not only in the central nervous system but also in peripheral tissues.

Objective: To assess the safety and efficacy of first-line bevacizumab combined with standard chemotherapy regimen in patients with advanced non-squamous non-small cell lung cancer. This report presents the result of a preplanned subanalysis of Shanghai Pulmonary Hospital's patients enrolled in SAiL study. Methods: Between July 2007 and March 2008, 44 patients with untreated locally advanced, metastatic, or recurrent non-squamous non-small cell lung cancer were recruited into this phase 4 study. Eligible patients had histologically or cytologically documented inoperable, locally advanced, metastatic, or recurrent disease (stages ?B-?); an Eastern Cooperative Oncology Group performance status score of 0-1; and adequate haematological, hepatic, and renal functions. Patients received bevacizumab (15 mg/kg every 3 weeks) plus standard chemotherapy (paclitaxel-carboplatin) every 3 weeks for up to six cycles, followed by single-agent bevacizumab until disease progression. The primary endpoint was safety, and the secondary end points included time to disease progression and overall survival. Results: The rates of clinically significant adverse events (= grade 3) of special interest were relatively low in this population (13.6%); proteinuria (7%), hypertension (2%) and bleeding (2%) were the most common adverse events. One patient died because of hemoptysis. The partial remission and stable disease rates were 52.3% (23/44) and 45.5% (20/44), respectively. One patient had progressive disease. The disease control rate was 97.7%. The median time to progression was 8.13 months, and the median overall survival was 17.73 months. Conclusion: The safety and efficacy of first-line bevacizumab-based treatment in Shanghai Pulmonary Hospital's patients with non-squamous non-small cell lung cancer is satisfying with benefits in time to progression and overall survival. There were no new safety signals to be reported. Copyright © 2013 by TUMOR.

Objective: To explore the effect of cognitive therapy on obsessional compulsive disorder (OCD). Method: Forty-seven patients with OCDO were randomly assigned to cognitive therapy group and conventional treatment group, the later with paroxetine treatment only as control. The results were assessed with Hamilton anxiety scale (HAMA), Yale-Brown Obsessional Compulsive Scale and social disability screening scale. Result: The cognitive therapy group had lower scores in HAMA and Y-BOCS than the control group after 8 weeks therapy, this difference remained in the follow-up after 6 months, when the cognitive group had also lower score in social disability. Conclusion: Cognitive therapy can benefit patients with OCD more than medication only.

Objective To evaluate the value of combined use of laparoscopy and hysteroscopy in the diagnosis of infertility. Methods Clinical data of 168 cases of infertility receiving examinations with laparoscopy and hysteroscopy from June 1999 to October 2003 were retrospectively reviewed.Results Hysteroscopic examinations found intrauterine diseases in 79 cases(79/168,47.0%),including 46 cases of endometrial hyperplasia or polyps(46/79,58.2%).Laparoscopic examinations showed organic pelvic diseases in 99 cases,including 85 cases of chronic pelvic inflammation,endometriosis or polycystic ovarian syndrome(85/99,85.9%).Both laparoscopy and hysteroscopy gave normal findings in 15 cases and abnormal findings in 39 cases.Unilateral or bilateral tubal obstruction was found in 90 cases by tubal patency tests under hysteroscope(90/168,53.6%) and in 78 cases by laparoscopy(78/168,46.4%). Conclusions Combined use of laparoscopy and hysteroscopy offers accurate diagnostic evidences in examinations of infertility.

Objective To clone, express and purify human PIF1 protein and analyze its ATPase activity. Methods The PIF1 cDNA was amplified by PCR from HeLa cell cDNA library and inserted to pET24b with histidine tag at its terminus to form pET24b-PIF1 plasmid. The recombinant pET24b-PIF1 plasmid was transformed to RosettaTM 2 (DE3) and the expression of PIF1 protein was monitored by SDS-PAGE analysis. By using fast protein liquid chromatograph (FPLC) system, the PIF1 protein was purified by affinity chromatograph and gel filtration. The ATPase activity of PIF1 was checked by thin layer chromatograph(TLC). Results The PIF1 protein was successfully cloned and expressed in E.coli. Conclusions The purification procedure of PIF1 protein was established using FPLC. The overexpressed and the purified PIF1 helicase has DNA and Mg2+ dependent ATPase activity.

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Objective To evaluate the effect of small dose ketamine on the efficacy of intravenous PCA (POA) with sufentanil after intra-abdominal surgery in aged patients.Methods Sixty ASA Ⅰ orⅡpatienm aged 65-82 yr undergoing elective intra-abdominal surgery under general anesthesia were randomized into 3 groups (n=20 each)according to the composition of PCIA solution:group I sufentanil 200 μg in 200 ml of noilnal saline (group S);group μ sufentanil 200μh+ketmine 100 mg in NS 200 ml(group K1)and group Ⅲ sufentannil 200 μg+ketmine 200 mg in NS 200 ml(group K2).A loading dose of 5 ml wag given at the end of operation.The PCIA setting was as follows:backgound infusion 1 ml/h,bolus dose 2 ml,lockout interval 5 min and 4-hour maximum dose 30 m1.If VAS score(0=no pain,10=womt pain)was≥7,pethidine 25 mg was given iv.The total amount of pethidine given within 48 h after operation and postoperative complications including nausea and vomiting and respiratory depression were recorded.Results Small dose ketamine added to the PCIA solution can significantly reduce the amount of pethidine administered after operation in a dose-dependent manner.Conclusion Small dose ketamine can improve the efficacy of PCIA with sufentanil after intra-abdominal surgery in aged patients with no significant adverse effect.

Acute Disease ; Adult ; Angioplasty, Balloon, Coronary ; adverse effects ; Humans ; Hypopituitarism ; etiology ; therapy ; Male

China ; epidemiology ; Hong Kong ; epidemiology ; Humans ; Microscopy, Electron ; SARS Virus ; genetics ; growth & development ; ultrastructure ; Severe Acute Respiratory Syndrome ; epidemiology ; prevention & control ; virology