AIM:To improve the standard of quality control of Yiqi Zhixue Granule(Radix Codonopsis,Radix Astragali,Rhizoma Atractylodis macrocephalae,etc.) METHODS: In the prescription,both Radix codonopsis and Radix Astragali were identified by HPLC.Folium mahoniae was distinguished by TLC.Rhizoma Bletillae was authenticated by microscope.Morever,RP-HPLC method was adopted to determine the astragaloside content in the productions,using a column of C_(18),the column temperature was at 35(?C),acetonitrile-water(32∶68) as a mobile phase,he flow rate was 1.0 mL/min,and ELSD as detection in which the tube temperature was set at 102(?C) and the air flow was at 2.8 mL/min,the impactor was chosen off. RESULTS: The established identification methods were simple and proper,reflecting a good specificity,while the determination method was accurate and reliable,which showed the recovery of 97.95%,RSD=2.21%(n=9),the intermediate precision of RSD=2.67%(n=3),the robustness of RSD=3.67%(n=3) between columns,and the LOQ of 0.539 2 ?g,RSD=2.92%(n=5). CONCLUSION: The improved standard of quality control of Yiqi Zhixue Granule is not only practical but capable of effectively controlling the quality of the medicine as well.

Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

Objective To study the relationship between the expression of p16 protein and the biological behavour of thyroid tumor. Methods The expression of p16 protein was examined in fifty cases of thyroid tumor by immunohistochemical staining technique(SABC method). The relationship between p16 protein expression and tumor size, pathological type, differention grading and clinical stage of thyroid tumors were analysed. Results In thyroid adenoma, p16 protein expression was detected in 13 out of 15 cases(86.7%); in thyroid carcinoma p16 protein was expressed only in 16 out of 35 cases(45.7%), the difference was significant(P

Etiological and serological testing, including virus genome, virus antigen, anti-virus antibodies, virus culture and identification, were the standard laboratory tests to confirm or exclude Ebola virus infections.Moreover, other laboratory tests, such as blood routine tests, liver and kidney function tests, serum electrolyte tests, and coagulation function tests, were very important to the clinical diagnosis and treatment of Ebola virus disease ( EVD) patients.Based on working experiences and practices in the China Ebola Treatment Center and available publications, opinions about the detailed clinical significance and associated key point of biosafty prevention and protection protocols of above described laboratory tests for the clinical diagnosis and treatment of EVD were provided.

In this paper , the author analyzed the network media with three main characteristics of negative re-ports about doctor -patient dispute , from four aspects explains the network media in building a harmonious doctor-patient relationship in ethics responsibility: public opinion guide , social responsibility , self-discipline to bear the ethical responsibility and abide by relevant laws and regulations , accept the heteronomy , points out that the net-work media should strengthen self -discipline and self -reflection are scientific and objective , guide the netizens rational appeal , to assume the social ethical responsibility of building a harmonious doctor -patient relationship .

〔Abstract〕 By retrieving the literatures from the official website of ONC , Tongfang Knowledge Network and the Internet , the paper conducts a survey of the conformance test and certification of health information standards in America from certification subject , certifica-tion object, test method and process, certification standard, certification mode and process, etc.It aims to provide a reference for the conformance test of health information standard in China .

Objective To study the clinical significance of interleukin (IL)-6 and IL-13 levels in serum of hand,foot,and mouth disease (HFMD)caused by enterovirus 71,and carries on the preliminary discussion on serum IL-6 and IL-13 cell origin. Methods Serum levels of IL-6 and IL-13 in HFMD and healthy control were detected by enzyme linked immunosorbent assay (ELISA),serum IL-6 and IL-13 levels among different severity of HFMD were compared.Serum IL-6 and IL-13 levels in HFMD after treatment were also detected.The secretion of IL-6 by monocyte and the secretion of IL-13 by T cells in peripheral blood mononuclear cells (PBMC)were detected by Flow Cytometry (FCM).Monocyte and T cells from health individual were treated with EV71,and the secretion of IL-6 by monocyte and the secretion of IL-13 by T cells were detected by FCM too.Results Serum levels of IL-6 and IL-13 were (79.63±29.45)pg/mL and (36.45±1 5.13)pg/mL respectively in HFMD,and serum levels of IL-6 and IL-13 were (27.26±7.82)pg/mL and (13.46±3.14)pg/mL respectively in health control,serum IL-6 and IL-13 levels in HFMD were significantly higher than that of healthy subjects (P < 0.01 ),and the mean serum IL-6 and IL-13 levels of HFMD were increased with the severity of the disease (P <0.01).After treatment,serum levels of IL-6 and IL-13 were (48.23 ±23.14) pg/mL and (23.25±9.63)pg/mL respectively,when compared with before treatment,serum levels of IL-6 and IL-13 were signifi-cantly decreased (P <0.01).The secretion ability of IL-6 by monocyte and the secretion of IL-13 by T cells in HFMD were signifi-cantly higher than that of healthy subjects.After infection by EV71,the secretion ability of IL-6 by monocyte and the secretion of IL-13 by T cells were significantly increased.Conclusion Infection of EV71 probably by increase the secretion ability of IL-6 by monocyte and the secretion of IL-13 by T cells,resulted in elevated of serum IL-6 and IL-13 levels,which involved in the occurrence and development of HFMD.

Objective To know the effects of electronic medical records(EMR) on the training quality of intern doctors' history-collecting and medical-records writing.Methods 195 students from Nantong University,Suzhou University and Xuzhou medical college were chosen as the subjects.For better effects,we divided the 195 students into two groups,81 students of Grade 2007 as the control group(hand-written medical record group) and 114 students of Grade 2008 as study group (electronic medical record group).The control group was arranged to complete EMR by transcribing the teacher's handwriting records into formal medical records after teachers' guidance and modification while the study group was arranged to write and directly modify the records on computer under the guidance of teachers and submit them for formal records.After the teaching,the medical record writing quality of two groups of interns was conducted centralized assessment to analyze the advantages and disadvantages of electronic medical records on the training of interns' collection and writing ability.SPSS 18.0 was used for statistical analysis,with count data using the number(percentage) and measurement data using mean +/-standard deviation.Measurement data of two groups of medical record writing scores were compared with t test,test level for α=0.05.Results The result of handwritten group was significantly better than EMR group(t=6.33,P＜0.001),which indicated training effectiveness of intern doctors' ability of writing medical record had digressive tendency on EMR group.Conclusion Negative effects of EMR are shown in intern doctors' history-collecting and medical-records writing.Cultivation quality of intern doctors' history-collecting and medical-records writing should be ensured by the combination of EMR and handwriting-medical training,the combination of daily inspection and key examination of medical record writing quality and establishment of feedback-rectification mechanism.

Objective To observe the clinical efficacy of acupoint injection at Renying (ST9) in treating cervical vertigo. Method Sixty-two patients were randomized into a treatment group and a control group. The treatment group was intervened by acupoint injection at Renying, while the control group was by regular acupuncture treatment. The clinical efficacy was evaluated after a treatment course. Result The total effective rate was 93.7% in the treatment group versus 80.0% in the control group, and the difference was statistically significant (P＜0.05);the symptom and function scores were significantly changed after intervention in both groups (P＜0.05). Conclusion Acupoint injection at Renying is significantly effective in treating cervical vertigo.

Objective To investigate the changes of thyroid hormones in pregnant women of different gestation by analyzing the test results from pregnant women .Methods Authors collected serum from 1 764 pregnant women and 121 healthy women(not pregnant ,control group) .And the levels of thyroid stimulating hormone(TSH) ,free triiodothyronine(FT3 ) and free thyroxine (FT4 ) in serum were tested by chemiluminescence microparticle immunization analysis(CMIA) method .Results The level of TSH (1 .06 mIU/L) in early-gestation women was lower than that in control group(P<0 .05) ,but increased in mid-gestation and late-gestation ,there was significant difference among early-gestation ,mid-gestation and late-gestation women(P<0 .05) .The levels of FT3 and FT4 in early-gestation women were higher than those in control group(P<0 .05) ,but decreased during mid-gestation and late-gestation ,there was significant difference among early-gestation ,mid-gestation and late-gestation women(P<0 .05) .Conclusion Thyroid hormones of pregnant women change in different gestation periods ,different reference values have to be adapted to evaluate thyroid gland function .