Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

AIM:To improve the standard of quality control of Yiqi Zhixue Granule(Radix Codonopsis,Radix Astragali,Rhizoma Atractylodis macrocephalae,etc.) METHODS: In the prescription,both Radix codonopsis and Radix Astragali were identified by HPLC.Folium mahoniae was distinguished by TLC.Rhizoma Bletillae was authenticated by microscope.Morever,RP-HPLC method was adopted to determine the astragaloside content in the productions,using a column of C_(18),the column temperature was at 35(?C),acetonitrile-water(32∶68) as a mobile phase,he flow rate was 1.0 mL/min,and ELSD as detection in which the tube temperature was set at 102(?C) and the air flow was at 2.8 mL/min,the impactor was chosen off. RESULTS: The established identification methods were simple and proper,reflecting a good specificity,while the determination method was accurate and reliable,which showed the recovery of 97.95%,RSD=2.21%(n=9),the intermediate precision of RSD=2.67%(n=3),the robustness of RSD=3.67%(n=3) between columns,and the LOQ of 0.539 2 ?g,RSD=2.92%(n=5). CONCLUSION: The improved standard of quality control of Yiqi Zhixue Granule is not only practical but capable of effectively controlling the quality of the medicine as well.

Objective To study the relationship between the expression of p16 protein and the biological behavour of thyroid tumor. Methods The expression of p16 protein was examined in fifty cases of thyroid tumor by immunohistochemical staining technique(SABC method). The relationship between p16 protein expression and tumor size, pathological type, differention grading and clinical stage of thyroid tumors were analysed. Results In thyroid adenoma, p16 protein expression was detected in 13 out of 15 cases(86.7%); in thyroid carcinoma p16 protein was expressed only in 16 out of 35 cases(45.7%), the difference was significant(P

Antibodies, Antineutrophil Cytoplasmic ; analysis ; immunology ; Antibodies, Antinuclear ; analysis ; immunology ; Antigens, Nuclear ; immunology ; Arthritis, Juvenile ; immunology ; Autoantibodies ; analysis ; immunology ; Humans ; Monitoring, Physiologic ; methods ; Nucleosomes ; immunology ; Phospholipids ; immunology ; Rheumatic Diseases ; immunology ; physiopathology

Specific fusion genes of leukemia are currently the main biomakers for minimal residual disease (MRD)monitoring. Recent studys have found that the WT1, PRAME, ERG genes may be the potential acute leukemia related molecules, and can be used to monitor acute myeloid leukemia's MRD, directing clinical stratified therapy and estimating prognosis. This article reviews the pathogenesis, incidence of these genes and the relationship to the clinical prognosis.

Objective To investigate the risk factors and clinical features of severe preeclampsia(SP) complicated with multiple organ dysfunction syndrome (Multiple Organ Dysfunction Syndrome,MODS) in order to provide reference for clinical practice.Methods Three hundred and ninety-one cases with severe preeclampsia were selected as our subjects,who hospitalized from February 2005 to February 2014 in our hospital.All subjects were divided into observation group (48 cases with MODS) and control group (343 cases without MODS).Clinical data were collected including age,gravidity,parity,gestational age,gestational age termination of pregnancy,uterine scar,prenatal care,medical history,treatment,mother child outcomes.Results There were significant differences between two groups in terms of weeks of termination of pregnancy,rate of uterine scar,rate of regular check-up of pregnancy,rate of chronic hypertension and renal disease.Logistic results showed that there were relationships between SP complicated with MO)S and the occurrence of termination of pregnancy with gestational age ＜28 weeks (OR =2.046,95％ CI:1.03 ～ 3.74,P =0.034),uterine scar (OR =2.401,95％ CI:1.16 ～ 4.09,P =0.008),non-regular prenatal care (OR =3.167,95％ CI:1.96 ～ 5.23,P =0.002),chronic hypertension (OR =3.348,95％ CI:2.23 ～ 5.78,P =0.001),renal disease (OR =3.064,95％ CI:1.77 ～ 5.18,P =0.038).The rate of underwent special treatment,preterm children,neonatal asphyxia,perinatal death,intracranial hemorrhage in observation group were 77.08％,79.17％,29.17％,20.83％,4.17％,higher than that in control group (23.62％,45.19％,13.70％,8.45％,1.17％ respectively; x2 =10.471,12.147,4.519,8.644,6.648,all P ＜ 0.05).The degree of organ injury score in gestational age ≥ 33 weeks and ＜ 33 weeks were (1.34 ± 0.28) points and (3.64 ± 0.69) points,and the difference was significant.The gestational week in gestational age ≥ 33 weeks and ＜33 weeks were (35.48 ±2.06) weeks and (29.87 ± 1.37) weeks,and the differencewas significant(t =3.917,P ＜0.05).The survival rates were 100％,77.78％,33.33％ when the numbers of damaged organs were 2,3 and ≥ 4.There was significant different between 2 and 3 damaged organs in terms of survival rate (x2 =3.964,P =0.024).Conclusion The risk factors of severe preeclampsia complicated with MODS include termination of pregnancy gestational age ＜ 28 weeks,uterine scar,non-regular prenatal care,chronic hypertension,renal disease.Prognosis of severe preeclampsia patients with MODs was poor than that without MODS and it relates with gestational age,the number of closely related organ damage.

Idiopathic thrombocytopenic purpura(ITP),which is the most common acquired hemorrhagic disease in childhood period,is also called immune thrombocytopenia because it is an immune-mediated disorder characterized by isolated thrombocytopehia.In recent years,the diagnosis and treatment of ITP has been dramatically improved,and some consensuses concerning the staging,symptom scoring,treatment indications and prognosis have been achieved.This article reviews the pathogenesis,epidemiology,symptom scoring,staging,grading of severity,treatment and prognosis evaluation of ITP in children.

Authors have examined burned patient's plasma for detecting and estimating titres of endotoxin with micro-limulus test.It was shown that low and high titres of endo-loxin were usually found in burned palients infected with gram-negative bacteria,especially Ps.aeruginosa, and with high body temperature.In patients suffering from septi-semia caused by gram-negative bacteria were usually found higher titres of endotoxin, which would endanger patients lives. Patients who were dying with a higher litre of endotoxin had the symptoms of endotoxic shock, such as a fall of body temperature and blood pressure.The micro-limulus test is a rapid, sensitive, reliable and reagent-saving in vitro method for quantitative estimation of endotoxin. It is much useful for sndotoxaemia diagnosis and helps to expedite treatment and prognosis.

After introcerebroventricular injection of kainic acid(KA) in the awake rat, three types of hippocampal unit responses, i. e- positive units, negative units and indifferent units, were recorded- Most positive units were hippocampal complex spike cells which correspond histologically to hippocampal pyramidal cells, and most hippocampal complex spike cells fired in positive bursts after KA treatment. In the early period after KA injection, the positive units were concentrated in CA3 area. It was suggested that:(1)the activities of positive units may be considered as the typical epileptiform hippocampal unit activity induced by KA, and the firing characteristics of the negative units may be the result of influence of hippocampal inhibitory interneurons or that of the excessive cellular depolarization;(2) the hippocampal pyramidal cells were more sensitive to the epileptogenic effect of KA than the hippocampal interneurons, and some pyramidal cells in CA3 area may serve as "pacemaker neurons" in KA induced epileptogenesis.

Professor XU Run-san has been dealt with Chinese medical practice for over 60 years,and was good at theory of pulse and syndrome differentiatiion. During the medical profession career,he concentrated to treating kinds of internal clinical diseases using old ancient formula and had outstanding effects. He claimed to combination Chinese medicine with western medicine in treating female illness. He cured some complicated female diseases successfully,such as dysfunctional uterine bleeding,pelvic inflammatory disease,and endometriosis. In clinic work,he insisted on combination differential diagnosis of diseases with differential syndrome of diseases,formula corresponding with syndrome of diseases.