Objective To investigate the changes and clinical indications of interleukin-2 receptor, ferritin and fi-brinectin in the cerebrospinal fluid of central nervous system leukemia.Methods ELISA was used to detect the changes of-soluble interleukin-2 receptor, ferritin and fibrinectin in the cerebrospinal fluid of central nervous system leukemia group ( CNSL) and non-CNSL group.Results The concentrations of soluble interleukin-2 receptor, ferritin and fibronectin ince-rebrospinal fluid of the CNSL group were significantly higher than those in non -CNSL group and the control group ( p <0.05).Conclusion The soluble interleukin 2 receptor, ferritin and fibronectin in cerebrospinal fluid are indicative of cen-tral nervous system leukemia.The result suggests that combined detection is important in early diagnosis of central nervous system leukemia.

Objective:To investigate the mutation of Brg1 gene and expression of Brg1 protein in gastric adenocarcinoma,and to study its relation with tumor development.Methods:By means of polymerase chain reaction-single strand conform action polymorphism(PCR-SSCP)37 cases of gastric mucosa were searched for Brg1 gene mutation in exon 4 and 16.By immunohistochemistry the expression of Brg1 protein in 30 cases of gastric adenocarcinoma was analyzed for their role in gastric cancer development and prognosis.Results:In 37 cases of gastric adenocarcinoma,no mutation of Brg1 gene was detected.Brg1 protein expression was 76.67% in gastric carcinoma which was higher than that in normal tissue(44.44%.) The expression of Brg1 was related to tumor growth,lymphnode metastasis and tumor differentiation.Conclusion:Genetic abnormalities of Brg1 might be rare and might not play a major role in stomach carcinogeneses.An increased expression of Brg1 protein was associated with the advanced stage of gastric carcinoma,the Brg1 expression can be used as an important indicator for differentiating malignancy and prognosis of gastric carcinoma.

This paper discusses the actuality of the artificial bioactive functionally gradient material and its developing trend.

To compare the efficacy and adverse effects of bortezomib+cyclophosphamide+dexamethasone (PCD) and vincristine+adriamycin+dexamethasone (VAD) regimens in multiple myeloma (MM). Methods:A total of 41 patients with MM were analyzed retrospectively and divided into two groups according to their treatment protocols:PCD group (21 cases) and VAD group (20 cases). Clinical effects and adverse effects were observed in both groups. Patients accepted two to four cycles of PCD or VAD regi-mens. Results:In the PCD group, three patients achieved complete remission (CR), three patients had very good partial remission (VG-PR), three patients were under partial remission (PR), eight patients had stable disease status (SD), and four patients had progressive dis-ease (PD). In the VAD group, none achieved CR, one patient had VGPR, two patients were under PR, nine patients had SD status, and nine patients had PD. The rate of patients who achieved efficacy (CR+VGPR+PR) in the PCD group was 42.9%, which was higher than that of the VAD group (15.0%). The rate of newly diagnosed patients who achieved good efficacy (CR+VGPR) in the PCD group was 50%, which was higher than that of the VAD group (7.7%). The incidence of herpes infection, cytopenia, fatigue, and gastrointestinal symptoms was similar in the two groups, whereas the incidence of neurotoxicity in the PCD group was higher than that of the VAD group. Conclusion:The response rate of PCD is higher compared with that of conventional VAD chemotherapy, especially in newly di-agnosed MM. PCD may improve CR and VGPR rates and may bring about more severe toxicities, such as neuropathy.

This paper summarized the application of Female Sexual Function Index (FSFI), and introduced its scoring system.

Objective To compare the results difference between the fluorescence staining and the acid fast staining (Ziehi‐Neelsen staining) methods in the detection of Mycobacterium tuberculosis ,and to compare the effects of the methylene blue solu‐tion ,Haris hematoxylin solution and potassium permanganate liquid as the redyeing reagents of the fluorescence staining method . Methods 198 sputum specimens collected from the patients with suspected tuberculosis symptoms and were performed the Ziehi‐Neelsen staining and the fluorescence staining respectively For comparing the difference in the detecting rate of Mycobacterium tu‐berculosis between the two kinds of method .The fluorescence staining adopted 0 .3% methylene blue solution ,0 .5% Haris hema‐toxylin solution and 0 .5% potassium permanganate solution as the redyeing reagents for comparing the effects of the fluorescence microscopic examination among different redying reagents .Results The detection rate of Mycobacterium tuberculosis was 66 .67%(132/198) for the Ziehi‐Neelsen staining ,94 .9% (188/198) for the fluorescence stainings and 94 .95% (188/198) for the methyl‐ene blue staining ,in which the detection rate of methylene blue redyeing was 94 .95% ,which of hematoxylin redyeing was 94 .44%(187/198) and which of potassium permanganate redyeing was 94 .44 (187/198) ,the differences among them were statistically sig‐nificant(P< 0 .05) .Conclusion The fluorescent staining method has the higher positive detection rate of Mycobacterium tubercu‐losis than the Ziehl‐Neelsen staining method ,in which 0 .3% methylene blue solution is a good background quenching redyeing solu‐tion .

Objective To study the new anticoagulants, felling shaaban joint care used in joint replacement for prevention of deep vein thrombosis.MethodsThe Second People's Hospital of Chengdu orthopaedics in March 2016 to March 2017 were hospitalized patients with knee joint replacement and total hip replacement of the 158 patients, according to the stochastic indicator method were randomly divided into observation group and control group, 79 cases in each group, the observation group received new anticoagulants, cutting class joint care, the control group accepted low molecular weight heparin calcium in combination with care.Observe two groups of treatment of deep vein thrombosis prevention effect and adverse reactions.ResultsCompared with control group, the observation group with lower incidence of DVT and difference comparing the two groups have statistical significance (P<0.05).Observation group compared with control group, PT, APTT, preoperative and postoperative 2 day, five days did not have obvious difference, no statistical significance.Observation group compared with control group, subcutaneous ecchymosis, incision hematoma bleeding had no obvious difference).ConclusionThe laval shaaban joint care to prevent deep venous thrombosis after artificial joint replacement effect of low molecular weight heparin calcium is better, and coagulation function effect is small.

Objective To investigate the risk factors for recurrence after surgery for biliary pancreatitis.Methods A total of 284 patients with biliary pancreatitis who were treated in The Fourth Hospital of PLA from January 2008 to December 2014 were followed up,and the risk factors for postoperative recurrence were analyzed.The chi-square test was used for categorical data,and the unconditional logistic regression model was used for multivariate analysis.Results The follow-up period ranged from 6 to 27 months (mean 36.4 ± 8.4 months).Of all patients,27 experienced recurrence,and the recurrence rate was 9.51％.The univariate analysis showed that postoperative recurrence was associated with a family history of gallstone disease,high-fat diet,sand-like stones,intrahepatic bile duct stones,biliopancreatic duct opening stenosis,and diverticulum around the ampullar region (x2 =8.721,5.979,8.641,15.996,33.833,and 27.203,all P ＜0.05).The multivariate logistic analysis showed that high-fat diet (OR =2.296,P =0.012),biliopancreatic duct opening stenosis (OR =2.280,P =0.007),and diverticulum around the ampullar region (OR =2.522,P =0.009) were independent risk factors for recurrence after surgery for biliary pancreatitis.Conclusion Biliary pancreatitis patients with high-fat diet,biliopancreatic duct opening stenosis,or diverticulum around the ampullar region tend to experience recurrence after surgery.Intervention and close follow-up should be performed for these patients to prevent recurrence.

Objective To analyze the clinical data,radiological and pathological features of breast cancer which were understated by image,and summarize the causes.Methods The clinical features,radiological features and pathological data of 26 cases of breast cancer were retrospectively analyzed.The preoperative ultrasound and mammography BI-RADS grades of these 26 cases were both ≤ 3,and they were confirmed to be breast cancer by pathology.The clinical and pathological data of 1224 cases of breast cancer in the same period were compared.Results For the 26 cases,100％ were early breast cancer,100％ were HER2 negative breast cancer,and only 1 case had lymph node metastasis.26％ were special type of breast cancer.Mammography showed glandular multi type,and ultrasound showed atypical or typical benign features.Conclusions Breast cancer understated by image shows features of young age,early stage,well differentiated,low malignant degree,not easy to be visualized by mammography,and their ultrasound features are usually atypical.In clinical setting,analysis of the ultrasound and mammography images should be combined with other examination,to decrease misdiagnosis rate.