BACKGROUND:The microenvironment of injured peripheral nerve was one of the most important factors that influence peripheral nerve regeneration. A favorable microenvironment for nerve regeneration would protect damaged neurons and promote axonal regeneration. OBJECTIVE:To simulate the microenvironment of peripheral neural regeneration with an amnion tube filled with polyglycolic polylactic acid(PLGA) filaments and autologous nerve tissue pulps and wrapped with a pedicled epimysium flap,and to study the feasibility to repair sciatic nerve defect. DESIGN,TIME AND SETTING:A randomized controlled study was performed in Experiment Animal Center of Guangdong Medical College from June 2006 to October 2007. MATERIALS:Thirty SD rats of clean grade and aged 2 months old were randomly divided into experimental group,control group,and standard group,with 10 rats in each group. The right side was operated,and the left side was control. Amnion was obtained from healthy,full-term,and spontaneous delivery fetus whose mothers provided the informed consent to establish amnion matrix membrane. Vicryl thread and amnion matrix membrane were used to make PLGA microfilament bridging graft. METHODS:The 6.0-mm sciatic nerve defect was bridged with different grafts,respectively:the amnion tube filled with Vicryl filaments and autologous nerve tissue pulps,and wrapped with a pedicled epimysium flap(experimental group) ;the amnion tube filled with autologous nerve tissue pulps(control group) ;autologous nerve(standard group) . MAIN OUTCOME MEASURES:Gross observation,histological examination,wet weight of the anterior tibial muscle,pass-through rate of myelinated nerve fibres and neural electrophysiology were assessed 8 and 12 weeks after the operation. RESULTS:At 12 weeks after operation,amyotrophy was lightly recovered in the experimental and standard groups except control group. Anterior tibial muscle at injured side was ruddy,well-stacked,and flexible in the experimental and standard groups,but the muscle was dark and poor elasticity. At 8 and 12 weeks after operation,there were significant differences in recovery rate of anterior tibial muscle between the three groups;in addition,at 12 weeks after operation,there were also significant differences in numbers of myelinated nerve fibers,cross-section area,vascular numbers of neural graft,vascular cross-section area,and compound muscle action potential amplitude of triceps surae between the three groups(P

Objective To study patients experience in and needs for vaginal douching in the department of gynecology.Method Using semi-structured interviews,10 patients undergoing vaginal douching in the hospital were selected,and then the interviews were analyzed and described with the content analysis method.Results Before vaginal douching,the patients had some psychological states of tension,fear,shyness,fear and calmness.In the process of vaginal douching,the patients experienced comforts,pain,fear,shyness and dissatisfaction with water temperature and skin preparation.After vaginal douching,the patients experienced worries of different sorts like vaginal infections,sexual life and pregnancy.The patients needed for the douching-related knowledge and the improvement in hygienic situations and they had some questions about urination and defection,operative effect and bathing and infection.Conclusion Medical staff should provide the corresponding services for patients during vaginal douching according to their needs and individual situations so as to enhance the nursing service quality.

Insulin receptor substrate(IRS)-1 and IRS-2 expression levels of liver tissues and skeletal muscle in intrauterine growth retarded(IUGR)rats were investigated by RT-PCR and immunohistochemistry.An IUGR animal model was established by maternal nutrition restriction during pregnancy.IRS-2 expression level of liver tissue and IRS-1 expression level of skeletal muscle in IUGR rats at 0 and 3 weeks old were significantly lower than those in normal rats at the same age respectively,and insulin resistance was induced in IUGR,and these findings might be the molecular mechanisms susceptible to metabolic syndrome in IUGR rats.

DQA1*0501.TSHR peptides of 183～198、195～210、248～263、301～320、343～362 and 357～376 could bind with high affinity to both HLA-DR3 and HLA-DQA1*0501,all of their IC50 values less than(1 ?mol/L),but they could bind to neither HLA-DR7 nor HLA-DQA1*0201 with high affinity.Conclusion Epitopes of TSHR 183～198,195～210,248～263,301～320,343～362 and 357～376 on the TSHR extracellular domain might be the auto-antigens to cause GD.

Objective To investigate the incidence and occurrence time of PICC-related upper extremity asymptomatic and symptomatic venous thrombosis in cancer patients;to explore the risk of asymptomatic venous thrombosis' developing into symptomatic venous thrombosis.Methods A total of 246 cancer patients who were scheduled to receive PICC between April and October 2014 were prospectively studied and divided into 2 groups by random digits table method.The time-phased follow-up observation on PICC cathertering cancer patients was conducted by Doppler ultrasound.The follow-up visiting inspection on the cathering upper limb vein by Doppler ultrasound at the 2nd,7th,14th,21st,28th,35th,42nd day were arranged after insertion of the catheter in experimental group (127 cases) to understand the incidence and occurrence time of asymptomatic venous thrombosis,while the control group (119 cases) were investigated for inspecting whether there were venous thrombosis through clinical symptoms or not.Once the clinical symptoms occurred,the incidence and time of occurrence of symptomatic venous thrombosis would be diagnosed by Doppler ultrasound and recorded.Results The incidence rate of asymptomatic thrombosis in the experimental group was 48.82％(62/127),the median time was 3 days,and there was no symptomatic thrombosis.Among them,85.48％(53/62) of the asymptomatic thrombosis occurred within 1 week,11.29％ (7/62) occurred in 2-3 weeks,and 3.23％ (2/62) occurred in 4-6 weeks after catheter insertion.Meanwhile,the incidence and median time of symptomatic thrombosis was 23.53％(28/119) and 12 days respectively in the control group.And 32.14％ (9/28) of these cases occurred within 1 week,50.00％ (14/28) occurred in 2-3 weeks,and 17.86％ (5/28) occurred in 4-6 weeks after catheter insertion.Conclusions The incidence of PICC-related upper extremity venous thrombosis in cancer patients is relatively high,and most cases have no symptoms and occur in an early time.Cancer patients are high-risk patients of venous thrombus.We should take early preventive intervention and treatment to reduce the incidence of venous thrombosis after catheter insertion.

10 years. Pulmonary functions were determined routinely and followed for 10 years. Results Comparison between the values of pulmonary functions at the beginning and end of a 10- year follow-up showed very significant difference in the groups of Ⅲ, Ⅳ and Ⅴ. Among the parameters examined, FEV_1, PEF, MMEF, and MVV showed significant increase in these 3 groups (P

OBJECTIVE:To establish a method for the uncertainty measurement evaluation of the content determination of pred-nisolone API. METHODS:HPLC internal standard method was adopted to determine the content of prednisolone API,establish mathematical model for uncertainty evaluation,analyze influential factors and evaluate the factors. RESULTS:HPLC internal stan-dard method showed the contont was 98.8%,and expanded uncertainty of prednisolone API was 1.6%,and the determination result was(98.8±1.6)%,k=2. CONCLUSIONS:The method is suitable for the uncertainty measurement evaluation of the content deter-mination of prednisolone API by HPLC internal standard method.

Aged ; Chordoma ; pathology ; Humans ; Male ; Nasal Cavity ; pathology ; Nose Neoplasms ; pathology

The antifungal mechanism of the TS67(Penicillum sp.) producing bioactive metabolites was investigated in this paper. The test indicated that mycelia growth, spores forming and spores germination of Bipolaris maydis and Fusarium oxysporum can be inhibitied by metabolites, after 50% fermentation broth acting 120 hours, the inhibition rate of hyphal growth can reach about 77.78%, 70.30%, the inhibition rate of spores forming also arrived at 58.8%, 73.5%; under 50% fermentation broth acting 12 hours, the inhibition rate of spores germination were up to 78.3%, 62.0%, the mycelia under activity metabolites conditoins also showed surface nodulation and growing point expanding irregularly, protoplasmic condesing, etc. Based on all these results, we may draw a conclusion that activity metabolites maybe target at fungal cell wall.

AIM: To analyze the effect of preoperative intravitreal injection of Ranibizumab ( lVR ) in patients undergoing pars plana vitrectomy ( PPV ) for proliferative diabetic retinopathy ( PDR) .
●METHODS:ln this retrospective research, 62 patients (67 eyes ) with PDR in the First Affiliated Hospital of Xinjiang Medical University from Jun. 2014 to Jun. 2015 were recruited. They were assigned to an experimental group ( n=37 eyes ) and a control group ( n=30 eyes ) . The patients in experimental group were given 1 injection of lVR ( Lucentis 1. 0mg/0. 1mL ) 1wk before surgery, whereas those in control group went down to surgery directly. The average operation time, iatrogenic breaks, the use of tamponade, electric coagulation, and vision increase rate were comparatively analyzed between two groups.
●RESULTS:The average operation time was (91. 7±20. 8) min in the experimental group vs (117. 6±18. 6)min in the PPV group(t = -5. 314,P<0. 05). The rate of electric coagulation (11%) vs (47%), (χ2 =8. 963, P=0. 006);iatrogenic breaks (5%) vs (33%), (χ2=8. 789, P=0. 004) and silicone oil or inert gas tamponade ( 27. 0%) vs (53. 3%), (χ2 = 4. 828, P = 0. 043 ). The rate of visual improvement (75. 7%) vs (46. 7%), (χ2 = 5. 960, P =0. 022 ) . There was no severe complication associated with surgery, such as choroidal detachment, retinal detachment and endophthalmitis.
●CONCLUSION:lVR before microincision vitrectomy can effectively shorten the operation time, reduce the use of electric coagulation and intraocular tamponade, and improve the rate of visual improvement for PDR patients.