For children suffering from primary nephrotic syndrome(PNS), podocyte is a crucial part of glomerular filtration barrier, whose injury usually causes proteinuria.The structural and functional integrity of podocyte cytoskeleton is the prerequisite of its normal physiological function.Recent studies demonstrated that under a certain pathological condition, B7-1, expressed in podocyte, can inhibit the activation of β1 integrin by competitively binding to the target site on the β1 integration, which may change the morphology and function of podocyte, consequently induced proteinuria.Other studies also have showed that abatacept, which selectively inhibit the cross-talk between B7-1 and β1-integrin,can reduce proteinuria in patients with B7-1-positive glomerular disease.These studies suggested that B7-1 may be a potential diagnostic biomarker and therapeutic target in proteinuria in PNS.This review summarizes the role of B7-1 expressed in podocyte in the pathogenesis of proteinuria and the possibility of clinical application in the future.

Objective:To prepare a quantified animal model of retinopathy of prematurity by economic and easy method. Methods:A total of 44 postnatal day 7 wistar rats were randomly assigned into 2 groups according to different oxygen inhalation concentration(n=22).Experimental group was exposed to 75% oxygen for 5 days,and them deprived of oxygen supply suddenly and exposured in air for 5 days.The Control group was exposed to room air in same experiment condition.The retinal vascular development and proliferation were evaluated by stretched preparation (ADPase stained retina)and eyeball cross-section,The expression of vascular endothelial growth factor(VEGF) was detected by immunohisto-chemical stain.Results: The central vessels became tortuous and constricted and the central avascular area increased in high-concentration oxygen environ-ment.But hypoxia environment made great vessels of retina broaden and abnormal curvature become obvious,neova- scularization was seen in the Experimental group.The average numbers of neovascular nuclei which breakthrough limiting membrane of amphiblestrodes in the Experimental group and the Control group were 32.800 0?0.767 8 and 2.750 0?0.716 4 respectively.Stain of VEGF was weaker in the control group than in the treated group.Conclusion:The rat modle of retinopathy of prematurity has powerful repeatability,high constancy and can be used in quantitative study.It is a suitable mode to study the pathogenesis and therapy of retinopathy of prema-turity.

Objective:To investigate the effects of hypoxia inducible factor-1?(HIF-1?)antisense oligonucleotides(ASODN)on the expression of vascular endothelial growth factor(VEGF)and retinal neovascularization in premature rat with retinopathy.Methods:A total of 80 postnatal day 7 wistar rats were randomly assigned into two group.The experimental group(n=40)were established as ROP mode group.The control group(n=40)was exposed to room air in the same experimental condition.HIF-1?ASODN was injected into rats'left cavum vitreum at 12、14、16 d in these two groups.Some of the experimental group were injected with saline at 14、16 d.So there were five groups of specimens including:normal eye(A),normal eye with HIF-1?ASODN(A1),ROP eye(B),ROP eye with HIF-1? ASODN(B1),ROP eye+saline(B2).The retinal vascular development and proliferation in five groups were evaluated by the retina stretch preparation of 15 newborn rats at 12、14、17 d respectively.Eyeball cross-section was staimed with hematoxylin and eosin(HE).The nuclei of proliferative retinal vessels of five groups were counted.The expression of VEGF and HIF-1?in retina by immunohisto-chemical stain were observed among all groups.Results:In ROP group(B)the central vessels became tortuous and constricted and the central avascular area increased at 12 d.At 14 d,neovascularization was seen,peaking at 17 d.ROP eye with HIF-1?ASODN(B1)presented the same as group A,but Neovascularization decreased obviously as compcured with group B.Retinal vessel shape of ROP eye+saline(B2)is same as group B.VEGF was mainly expressed in capillary endothelium endochylema and nucleus of ganglionic layer of retina and inner nuclear layer.The expressions of VEGF were weaker in group A, A1 than in group B,B2,with significant differences(P

OBJECTIVE To study the occurrence of patients′ respiratory system infection in ICU and its incidence influenced by nursing interference. METHODS The incidence of respiratory system infection of patients who were in ICU longer than 48 hours. were analyzed the normal nursing group with 461 cases from Jan to Dec 2006 were compared with the normal nursing with interference group with 490 cases from Jan to Dec 2007. So the results of two groups were compared by statistical analysis. RESULTS Twelve from 461 cases (the incidence of 2.6%) in normal nursing group had respiration system infection,vs 6 from 490 cases incidence of 1.2% in normal nursing with interference group. With nursing interference the incidence of patients′ respiration infection decreased obviously. There was significant difference between two groups (P

Objective In order to make clear the clinical characteristics of gallbladder carcinoma.Method 37 cases of gallbladder carcinoma treated in Xinhua hospital have been analyed in recent 5 years.Result The higher incidence was in the elderly and the ratio between femal and male is 2 1 to 1.Clinical symptoms include right upper abdominal pain,nausea,fever,jaundice and abdominal mass.With gallstones is about 81 1%.Conclusion BUS and CT are common diagnostic methods.BUS is more reliable in early case,but CT in late case.The type of operation and the result of treatment are dependent on the pathologic stage and grade.

Objective:To investigate the curative effect of Chinese medicine foot bath combined with foot massage on diabetic peripheral neuropathy (DPN).Methods:Seventy-six patients with DPN who received treatment in Lishui Hospital of Traditional Chinese Medicine from July 2018 to December 2019 were included in this study. They were randomly assigned to undergo either Chinese medicine foot bath combined with foot massage (combined treatment group, n = 38) or Chinese medicine foot bath alone (single treatment group, n = 38) for 4 successive weeks in addition to diet and exercise management, medication for lowing blood glucose level, microcirculation improvement, and neurotrophic treatment. Before and after treatment, Toronto Clinical Neuropathy Score, Michigan Diabetic Neuropathy Score, and combined foot score were compared between the combined treatment and single treatment groups. The curative effect was also compared between the two groups. Results:After 4 weeks of treatment, Toronto Clinical Neuropathy Score [(5.82 ± 0.57) points, (7.62 ± 0.89) points], Michigan Diabetic Neuropathy Score [(14.92 ± 2.16) points, (18.01 ± 2.87) points], combined foot score [(3.17 ± 0.49) points, (4.41 ± 0.52) points] in the combined treatment and single treatment groups were significantly lower than those before treatment [(9.84 ± 1.27) points, (9.65 ± 1.21) points, (23.09 ± 4.03) points, (22.81 ± 3.92) points, (5.62 ± 0.85) points, (5.76 ± 0.73) points, t = 3.137, 2.361, 2.952, 2.392, 3.341, 2.427, all P < 0.05]. The declines in the combined treatment group were significantly greater than those in the single treatment group (all P < 0.05). Total effective rate in the combined treatment group was significantly higher than that in the single treatment group (94.74% vs. 78.95%, χ2 = 4.150, P < 0.05). Conclusion:Chinese medicine foot bath combined with foot massage is effective for DPN, which can significantly improve the clinical symptoms and promote the recovery of neurological function.

Objective To study the expression of toll-like receptor 4 (TLR4), nuclear factor κB (NF-кB) and Caspase-3 and the change of neuron apoptosis of the hippocampus in the status convulsion rat , and to explore the effect of resveratrol on them.Methods A total of 106 male Sprague-Dawley(SD)rats were randomly divided into the control (A), convulsion (B) and resveratrol treatment (C) groups.Group B was further randomly divided into four subset groups (B1-B4) which were executed at 4h, 24h, 48h, 72h after convulsion discontinued .Continuous epilepticus was induced by injecting lithium chloride and pilocarpine , and group C was daily injected with 30mg/kg resveratrol 30minutes after convulsion stopped for 3 days.TLR4 and NF-κB/p65 proteins were detected by immunohistochemistry ( IHC ); the expressions of TLR4 and Caspase-3 mRNA were detected by RT-PCR.The neuron apoptosis was observed by TUNEL . Results The IHC staining of TLR4 protein in group B was significantly higher than in group A (P<0.05), and that in group C was much lower than in B 4 group ( P<0.01 ) .The expression of NF-кB/p65 showed that hippocampal neurons had positive expression in cell nuclei in group B compared with group A (P<0.05), and the expression of NF-кB/p65 protein in group C was much lower than that in group B 4 (P<0.01).The mRNA expressions of TLR4 and Caspase-3 in the rat hippocampus of group B were significantly elevated than that in group A ( P <0.05 ) , and the tendency was increased gradually , reaching the peak at 72 hours after seizure , and that in group C was much lower than that in B 4 group (P<0.01).The TUNEL positive cells in hippocampus CAl of B group were more than that of group A after the SC 24hours (P<0.01),reached the highest level at the 72nd hour, and that in group C the TUNEL positive cells were lower than that in B4 group (P<0.01).Conclusion The expression of TLR4, NF-кB and Caspase-3 increased after SC.Resveratrol can down regulate the expression of TLR4, NF-кB and Caspase-3 in the hippocampus with pilocarpine-induced seizures, reduced the number of neuronal apoptosis .These results suggest that resveratrol may have a protective effect against the hippocampus damage caused by status convulsion .

Objective To establish the model of alcohol-induced hepatocyte steatosis in L-02 liver cells in vitro,investigate the relationship between cell damage,UCP2 gene expression and ethanol concentrations in L-02 cells of steatosis,and explore the protective effect of Hovenia Dulcis Thunb extracted liquid.Method Hovenia Dulcis Thunb extracted liquid at the most compatible dosage was applied to the L-02 cells of steatosis.Then UCP2 mRNA was detected by the semi-quantitative RT-PCR method.Results In steatosis model groups,UCP2 mRNA expression was downregulated ( P ＜0.05).In the steatosis groups treated with Hovenia Dulcis Thunb extracted liquid,UCP2 mRNA level was significantly increased compared to steatosis group ( its expression level increased from 0.4859 to 0.7442 after treatment of 0.6％ ethanol,P＜ 0.05).Conclusions Ethanol can down-regulate the UCP2 mRNA expression of L-02 cells in a certain period.The low expression of UCP2 mRNA is probably relevant to the occurrence of alcoholic fatty liver disease.Hovenia Dulcis Thunb can prevent from the cell toxicity of ethanol and decrease the degree of steatosis.Hovenia Dulcis Thunb extracted liquid can increase the expression level of UCP2 mRNA in the L-02 cells.

Objective To investigate the correlation between benign prostate hyperplasia(BPH) and atherosclerotie related indicators.Methods Totally 125 patients were divided into two groups based on prostate volume(PV):50 cases without BPH (PV≤30 ml) and 75 cases with BPH (PV＞30 ml).Age,body mass index (BMI),triglycerides (TG),total cholesterol,low-density lipoprotein cholesterol,high-density lipoprotein cholesterol,glycosylated hemoglobin,prostate specific antigen (PSA),HOMA-IR,bilateral ankle brachial index (ABI) and cardio-ankle vascular index (CAVI) were measured and calculated.All these indicators were compared between two groups.The correlations of these indicators with PV were analyzed.Results BMI,TG,PSA,HOMA-IR and right-CAVI in BPH group were higher than in group without BPH (t=2.177,2.033,3.060,2.859,3.085,all P＜0.05),and right ABI was lower in the BPH group than in group without BPH (t=2.557,P＜0.05).The incidences of diabetes and coronary diseases in BPH group were increased compared with those without BPH (52％ and 36％ vs.30％ and 10％,x2 =5.917,10.648,P =0.015,0.001,respectively).PV was positively correlated with BMI,TG,PSA,right CAVI,left CAVI(r=0.336,0.216,0.556,0.295,0.389,all P＜0.05)by Pearson analysis.Conclusions BPH is closely correlated with insulin resistance and atherosclerosis.Atherosclerosis might cause prostate chronic ischemia and hypoxia,which may play a role in the pathogenesis of BPH.

Objective To evaluate and compare the therapeutic effects among intravenous rt-PA thrombolysis,thrombolysis plus delayed intracoronary stenting for acute myocardial infaction(AMI)using 99Tcm-MIBI myocar dial SPECT imaging.Methods 60 cases of AMI were divided into two groups Which were the thrombolysis group(n=30),thrombolysis plus delayed stenting group(n=30).99Tcm-MIBI myocardial SPECT imaging was performed 3 weeks after thrombolysis therapy.Results The scores of the two groups were(29.5±7.8),(18.4±6.2)(t=7.2,P＜0.01)respectively.Conclusion Thrombolysis plus delayed stenting seems to be more effective than thrombolysis.