Objective:To prepare a quantified animal model of retinopathy of prematurity by economic and easy method. Methods:A total of 44 postnatal day 7 wistar rats were randomly assigned into 2 groups according to different oxygen inhalation concentration(n=22).Experimental group was exposed to 75% oxygen for 5 days,and them deprived of oxygen supply suddenly and exposured in air for 5 days.The Control group was exposed to room air in same experiment condition.The retinal vascular development and proliferation were evaluated by stretched preparation (ADPase stained retina)and eyeball cross-section,The expression of vascular endothelial growth factor(VEGF) was detected by immunohisto-chemical stain.Results: The central vessels became tortuous and constricted and the central avascular area increased in high-concentration oxygen environ-ment.But hypoxia environment made great vessels of retina broaden and abnormal curvature become obvious,neova- scularization was seen in the Experimental group.The average numbers of neovascular nuclei which breakthrough limiting membrane of amphiblestrodes in the Experimental group and the Control group were 32.800 0?0.767 8 and 2.750 0?0.716 4 respectively.Stain of VEGF was weaker in the control group than in the treated group.Conclusion:The rat modle of retinopathy of prematurity has powerful repeatability,high constancy and can be used in quantitative study.It is a suitable mode to study the pathogenesis and therapy of retinopathy of prema-turity.

Objective:To investigate the effects of hypoxia inducible factor-1?(HIF-1?)antisense oligonucleotides(ASODN)on the expression of vascular endothelial growth factor(VEGF)and retinal neovascularization in premature rat with retinopathy.Methods:A total of 80 postnatal day 7 wistar rats were randomly assigned into two group.The experimental group(n=40)were established as ROP mode group.The control group(n=40)was exposed to room air in the same experimental condition.HIF-1?ASODN was injected into rats'left cavum vitreum at 12、14、16 d in these two groups.Some of the experimental group were injected with saline at 14、16 d.So there were five groups of specimens including:normal eye(A),normal eye with HIF-1?ASODN(A1),ROP eye(B),ROP eye with HIF-1? ASODN(B1),ROP eye+saline(B2).The retinal vascular development and proliferation in five groups were evaluated by the retina stretch preparation of 15 newborn rats at 12、14、17 d respectively.Eyeball cross-section was staimed with hematoxylin and eosin(HE).The nuclei of proliferative retinal vessels of five groups were counted.The expression of VEGF and HIF-1?in retina by immunohisto-chemical stain were observed among all groups.Results:In ROP group(B)the central vessels became tortuous and constricted and the central avascular area increased at 12 d.At 14 d,neovascularization was seen,peaking at 17 d.ROP eye with HIF-1?ASODN(B1)presented the same as group A,but Neovascularization decreased obviously as compcured with group B.Retinal vessel shape of ROP eye+saline(B2)is same as group B.VEGF was mainly expressed in capillary endothelium endochylema and nucleus of ganglionic layer of retina and inner nuclear layer.The expressions of VEGF were weaker in group A, A1 than in group B,B2,with significant differences(P

OBJECTIVE To study the occurrence of patients′ respiratory system infection in ICU and its incidence influenced by nursing interference. METHODS The incidence of respiratory system infection of patients who were in ICU longer than 48 hours. were analyzed the normal nursing group with 461 cases from Jan to Dec 2006 were compared with the normal nursing with interference group with 490 cases from Jan to Dec 2007. So the results of two groups were compared by statistical analysis. RESULTS Twelve from 461 cases (the incidence of 2.6%) in normal nursing group had respiration system infection,vs 6 from 490 cases incidence of 1.2% in normal nursing with interference group. With nursing interference the incidence of patients′ respiration infection decreased obviously. There was significant difference between two groups (P

For children suffering from primary nephrotic syndrome(PNS), podocyte is a crucial part of glomerular filtration barrier, whose injury usually causes proteinuria.The structural and functional integrity of podocyte cytoskeleton is the prerequisite of its normal physiological function.Recent studies demonstrated that under a certain pathological condition, B7-1, expressed in podocyte, can inhibit the activation of β1 integrin by competitively binding to the target site on the β1 integration, which may change the morphology and function of podocyte, consequently induced proteinuria.Other studies also have showed that abatacept, which selectively inhibit the cross-talk between B7-1 and β1-integrin,can reduce proteinuria in patients with B7-1-positive glomerular disease.These studies suggested that B7-1 may be a potential diagnostic biomarker and therapeutic target in proteinuria in PNS.This review summarizes the role of B7-1 expressed in podocyte in the pathogenesis of proteinuria and the possibility of clinical application in the future.

Objective In order to make clear the clinical characteristics of gallbladder carcinoma.Method 37 cases of gallbladder carcinoma treated in Xinhua hospital have been analyed in recent 5 years.Result The higher incidence was in the elderly and the ratio between femal and male is 2 1 to 1.Clinical symptoms include right upper abdominal pain,nausea,fever,jaundice and abdominal mass.With gallstones is about 81 1%.Conclusion BUS and CT are common diagnostic methods.BUS is more reliable in early case,but CT in late case.The type of operation and the result of treatment are dependent on the pathologic stage and grade.

Objective:To investigate the curative effect of Chinese medicine foot bath combined with foot massage on diabetic peripheral neuropathy (DPN).Methods:Seventy-six patients with DPN who received treatment in Lishui Hospital of Traditional Chinese Medicine from July 2018 to December 2019 were included in this study. They were randomly assigned to undergo either Chinese medicine foot bath combined with foot massage (combined treatment group, n = 38) or Chinese medicine foot bath alone (single treatment group, n = 38) for 4 successive weeks in addition to diet and exercise management, medication for lowing blood glucose level, microcirculation improvement, and neurotrophic treatment. Before and after treatment, Toronto Clinical Neuropathy Score, Michigan Diabetic Neuropathy Score, and combined foot score were compared between the combined treatment and single treatment groups. The curative effect was also compared between the two groups. Results:After 4 weeks of treatment, Toronto Clinical Neuropathy Score [(5.82 ± 0.57) points, (7.62 ± 0.89) points], Michigan Diabetic Neuropathy Score [(14.92 ± 2.16) points, (18.01 ± 2.87) points], combined foot score [(3.17 ± 0.49) points, (4.41 ± 0.52) points] in the combined treatment and single treatment groups were significantly lower than those before treatment [(9.84 ± 1.27) points, (9.65 ± 1.21) points, (23.09 ± 4.03) points, (22.81 ± 3.92) points, (5.62 ± 0.85) points, (5.76 ± 0.73) points, t = 3.137, 2.361, 2.952, 2.392, 3.341, 2.427, all P < 0.05]. The declines in the combined treatment group were significantly greater than those in the single treatment group (all P < 0.05). Total effective rate in the combined treatment group was significantly higher than that in the single treatment group (94.74% vs. 78.95%, χ2 = 4.150, P < 0.05). Conclusion:Chinese medicine foot bath combined with foot massage is effective for DPN, which can significantly improve the clinical symptoms and promote the recovery of neurological function.

Objective To study the expression of toll-like receptor 4 (TLR4), nuclear factor κB (NF-кB) and Caspase-3 and the change of neuron apoptosis of the hippocampus in the status convulsion rat , and to explore the effect of resveratrol on them.Methods A total of 106 male Sprague-Dawley(SD)rats were randomly divided into the control (A), convulsion (B) and resveratrol treatment (C) groups.Group B was further randomly divided into four subset groups (B1-B4) which were executed at 4h, 24h, 48h, 72h after convulsion discontinued .Continuous epilepticus was induced by injecting lithium chloride and pilocarpine , and group C was daily injected with 30mg/kg resveratrol 30minutes after convulsion stopped for 3 days.TLR4 and NF-κB/p65 proteins were detected by immunohistochemistry ( IHC ); the expressions of TLR4 and Caspase-3 mRNA were detected by RT-PCR.The neuron apoptosis was observed by TUNEL . Results The IHC staining of TLR4 protein in group B was significantly higher than in group A (P<0.05), and that in group C was much lower than in B 4 group ( P<0.01 ) .The expression of NF-кB/p65 showed that hippocampal neurons had positive expression in cell nuclei in group B compared with group A (P<0.05), and the expression of NF-кB/p65 protein in group C was much lower than that in group B 4 (P<0.01).The mRNA expressions of TLR4 and Caspase-3 in the rat hippocampus of group B were significantly elevated than that in group A ( P <0.05 ) , and the tendency was increased gradually , reaching the peak at 72 hours after seizure , and that in group C was much lower than that in B 4 group (P<0.01).The TUNEL positive cells in hippocampus CAl of B group were more than that of group A after the SC 24hours (P<0.01),reached the highest level at the 72nd hour, and that in group C the TUNEL positive cells were lower than that in B4 group (P<0.01).Conclusion The expression of TLR4, NF-кB and Caspase-3 increased after SC.Resveratrol can down regulate the expression of TLR4, NF-кB and Caspase-3 in the hippocampus with pilocarpine-induced seizures, reduced the number of neuronal apoptosis .These results suggest that resveratrol may have a protective effect against the hippocampus damage caused by status convulsion .

Objective To investigate the effects of bisphosphonate on the proliferator-activated receptor γ(PRARγ)expression of glucocorticoid-induced osteoporosis. Methods ① Twenty-two cases of systemic lupus erythematosus(SLE)patients with glucocorticoids(＞0.5 mg·kg-1 ·d-1)were divided into 2 groups: 9 cases in the treatment group in which patients received alendronate 70 mg qw., 13 cases in the control group without anti-osteoporosis treatment. All of the patients had bone marrow puncture after 24 weeks, bone tissues were embedded, sliced, and the value of average optical density of PPARγwas tested with immunohistochemistry. ② Human bone mesenchymal stem cells were cultured in the same conditions and stimulated to adipocytes. During the adipogenic process, the cells were divided into four groups, three of which were experimental groups treated with 10-5 mol/L(high-dose group), 10-7 mol/L(medium-dose group)and 10-9 mol/L(low-dose group)bisphosphonate respectively, while the other was the control group without bisphosphonate in the medium. The adipocytes were identified by oil Red O stain seven days later. The adipocyte rate was measured by light absorption at 515 nm. The expression of PPARγ was measured by quantitative PCR. Comparisons between groups were tested by One-Way ANOVA analysis and t test. Results The average optical density of PPAR-gamma immunohistochemistry in the treatment group was less than the control group(P＜0.05). Seven days later, the absorbance between adipogenic induction group and low-dose group was not significant(P=0.167). but the adipogenic absorbance between the high-dose group, middle dose group and the control groups(P values were 0, 0.041). There was significant difference in the quantitative PCR results between the high-dose group, middle dose group and the expression of PPARγ adipogenic induction group(P value was 0, 0.01), but there was no significant differences between the low-dose group and adipogenic induction group(P＞0.05). Conclusion Effective concentrations of bisphosphonate can inhibit the expression of PPARγ which in turn lead to the suppression of hMSCs to the adipocytes. The glucocorticoid-induced osteoporosis is also depressed.

OBJECTIVE: To systematically understand natural philosophical medical mode that is the important component part of theoretical medicine studies, to further explain its theoretical values and application significance, so as to promote the constant improvement of medical mode.DATA SOURCES: Based on Social Medicine---Evolution of Medical Mode---Empirical Medical Mode, synthesized the features of TCM, Chinese thought history, Chinese cultural history and the research method features, puts forward to the systematic understanding of natural philosophical medical mode.STUDY SELECTION: Data was selected from social medical data, TCM data, Chinese cultural data and thought history data. The selected data can embody outlook of nature, abstract thinking features and mode features.The data was analyzed and grouped to reveal the application of the thoughts and methods of natural philosophy in the understanding of health and diseases.DATA EXTRACTION: Among 23 papers, the data was mainly selected from 6 papers including Shanggu Tianzhen Lun, Shengqi Tongtian Lun and Zangqi Fa Shi Lun. The thoughts, theory and understanding from these materials are used to expound the natural and social phenomena of the human body, and get the conclusion that the qualitative analysis is sufficient,while the quantitative concepts are scanty; subjective experiences and clinical disproof theory are plentiful while concrete proof analysis and morphological injury principles are scarce.DATA SYNTHESIS: Based on the influences of holism and methodology of natural philosophy, the natural philosophical medical mode can only grasp the life phenomena organically. It attaches importance to the generalization of the relationships and phenomena above the level of the human body, but pays less attention to the concrete structure analysis below the level of human body. Therefore it emphasizes the interactive roles of the four-dimension structure of the heaven, earth, people and ethics in human health and diseases, invented many difficult material concepts to specify the physiological and pathological concepts. The life principles are invariable, the content of qi mechanism remains constant. All diseases can be understood, syndrome-differentiated and presc ribed. However the medical concepts are mostly cited from ancient philosophical concepts and the general level of medical theory remains in this level of thoughts.CONCLUSION: Natural philosophical medical mode centers upon social science, social phenomena and social psychology as its main tool. It attaches more importance to the essential attribute of man, namely the social attribute. By contrast the biological medical mode attaches importance to the natural attribute of man. namely the biological attribute. The two factors constitute the two sides of one thing. Compared with the present medical mode of biology---psychology---society, the natural philosophical medical mode not only has its systematic nature and holism, but also clinical therapeutic theory and more operational nature. It may enlighten people's wisdom and is an art of understanding health and diseases. Thus it possesses very important research significance and clinical application values.

The present research aims to point out the long-existing defect of analyzing the spectrum diagram only from the perspective of haemodynamics instead of haemorheology. In the light of the theories of haemodynamics and haemorheology, the causes of spectrum diagram formation of carotid artery blood at the rapid and slow flow can be clarified completely and accurately. Four conclusions have been drawn in the end. As long as the velocity gradient is large enough, obvious red blood cells concentrate to the shaft even in the big or bigger blood vessels; the spectrum diagram is the powerful proof of the two phase flow model of blood; the spectrum diagram can be completely and accurately analyzed only by combining haemodynamics with haemorheology; and only when the red blood cells concentrate to the shaft, the big or bigger blood vessels can be regarded as haemogeneous fluid.
Blood Flow Velocity ; Carotid Arteries ; diagnostic imaging ; Hemodynamics ; Hemorheology ; Humans ; Ultrasonography, Doppler