ObjectiveTo explore the current occurrence of upper gastrointestinal bleeding in elderly patients in our hospital and its possible risk factors and treatment program.MethodsAn observational prospective analysis of medical records of 758 consecutive patients was performed.Clinical presentation,etiology and treatment were evaluated.The follow-up ended on the dates of a first occurrence of UGIB,stopping co-therapy,death or 6 months after admitted in the group.Results426 male and 332 female were admitted in the group,UGIB occurred in 48 patients.PPI,H2RA,gastric mucosa protectant plus PPI,gastric mucosa protectant plus H2RA and control were prescribed in 108,240,70,102,238 patients respectively.There were statistics difference in each group compared with control in bleeding rate.ConclusionThe occurrence of UGIB associated with A + C co-therapy in elderly was 6.3％.Co-prescription with PPI and gastric mucosa protectant was associated with a lower risk.

Age-related macular degeneration (AMD) is one of the most important diseases that cause vision loss among older patients in developed countries.As the progress of population aging in China,the incidence of AMD is increasing.The activation of the complement system plays an important role in AMD pathogenesis.Complement factor Ⅰ (CFI) is an important complement regulator.In recent years,there have been numerous studies about CFI single-nucleotide polymorphisms (SNPs) and AMD.The mechanism of CFI and the association between CFI polymorphisms and AMD were reviewed in the article.

Objective To monitor and analyze the use of non-national immunization program(nNIP) vaccines in Fujian Province from 2014 to 2020,and provide a reference for improving the management of nNIP vaccination service.Methods The nNIP vaccination data from 2014 to 2020 were extracted from the National Immunization Program Information System(NIPIS),and analyzed by descriptive epidemiological method.Results From 2014 to 2020,a total of 28 835 834 doses of nNIP vaccines were administered in Fujian Province,with an average of 1 015 doses per 10 000 population,and the varieties used increased from 22 to 28.The annual administered doses of nNIP vaccine,the proportion in total doses of all vaccines and the number of vaccinations per capita basically increased year by year.The most widely used varieties mainly include varicella attenuated live vaccine(VarV),hepatitis B(HepB) vaccine,rabies vaccine(RabV) for human use,haemophilus influenza type b(Hib) polysaccharide conjugate vaccine,influenza vaccine(InfV) and enterovirus 71(EV71)inactivated vaccine.The three vaccines with the highest replacement rates were hepatitis A vaccine,group A meningococcal vaccine and group A and C meningococcal vaccine,with the replacement rates of 22.42%,10.32% and 9.89%respectively.Conclusion There are more and more types and doses of nNIP vaccines used in Fujian Province,so it is necessary to further strengthen monitoring and management and improve vaccination service.

Objective To study treatment has no risk factors associated with response to treatment with intravenous gamma globulin (IVIG)protein in children with Kawasaki disease,so as to provide basis for clinical early treatment scheme.Methods The clinical data of 187 cases of Kawasaki disease in children were retrospective ana-lyzed.Patients were divided into sensitive group (160 cases)and non sensitive group (27 cases)according to the sensitivity of IVIg treatment,differences in baseline data were compared between the two groups of children admitted to hospital.Related factors were screened by clinical manifestations and logistic regression analysis.Results In chil-dren of non sensitive group,white blood cell count (WBC),neutrophil cell count (N),hemoglobin (HB),C -reac-tive protein (CRP),erythrocyte sedimentation rate (ESR),albumin (ALB),total bilirubin (TB)and immunoglo-bulin (IgG)values were determined respectively of (18.4 ±5.4)×109 /L,(0.72 ±0.16)×109 /L,(99.2 ±11.3)g/L, (108.4 ±43.2)mg/L,(75.5 ±21.8)mm/h,(29.8 ±3.1)g/L,(21.6 ±9.3)μmol/L and (11.8 ±3.4)g/L.The data of sensitive group were (15.1 ±4.3)×109 /L,(0.62 ±0.13)×109 /L,(109.5 ±10.4)g/L,(73.5 ± 41.5)mg/L,(55.3 ±19.4)mm/h,(33.8 ±3.5)g/L,(12.9 ±8.8)μmol/L and (7.6 ±3.5)g/L.The difference between the two groups was statistically significant (t =3.548,3.570,4.701,4.018,4.915,5.578,4.713,5.791,all P <0.05).Logistic regression analysis results showed that ESR (OR =2.083),TB (OR =1.632),CRP (OR =1.592)were three main risk factors of IVIG in the treatment of children with Kawasaki disease,and high Hb (OR =0.743),Alb (OR =0.447)could reduce the risk of IVIG treatment.Conclusion In the first visit,higher WBC,N, CRP,ESRTBand IgG levels may increase the reactive risk of IVIG treatment in children with Kawasaki disease.

Objective To improve the understanding of X-ray expression of chest in uremia.Methods X- ray expression of chest in 40 cases with uremia were analysed retrospectively.Results 18 eases were interstitial ede- ma of lung,5 cases alveolar edema,17 cases thoracic effusion,32 cases heart image enlargement.Conclusion Chest x-ray film is the convenient and effective method in diagnosis of the chest expression abnormity of uremia and it can guide the clinical treatment of cases with uremia.

Through systematical isolation and purification of cellulase from Aspergillus niger using the technique of (NH_4)_2SO_4 fraction ation, gel filtration and ion exchange chromatography, we obtained seven components of endo-?-glucanase (Ⅰ-2a,Ⅰ-2b,Ⅰ-2c, Ⅰ-2d, Ⅱa, Ⅱb, Ⅲa) All of them showed homogeneity in the dise gel electrophresis.

Objective To assess the effects of Baclofen plus comprehensive rehabilitation therapy in treating patients with hemiplegic muscle spasticity after cerebral trauma and stroke. Methods Thirty-four patients were divided randomly into two groups. Seventeen patients of therapy group were treated by combining Baclofen and comprehensive rehabilitation therapy, and 17 patients of control group were treated only with comprehensive rehabi-litation therapy. The muscular tension and self-care ability between pretreatment and post-treatment in two groups were assessed by Modified Ashworth Scale and Modified Barthel Index. Results The muscular tension was signi-ficant decreased in patients after treatment ~P

OBJECTIVE:To study the effect of surfactants on the pharmaceutical index of sucralfate capsule and to opti?mize its dosage used in the formula.METHODS:The sucralfate capsule was prepared with different surfactants respectively.The disintegration,dissolution and dispersibility of the preparations were determined and their stripping curves were fitted with Weibull distribution model.Different surfactants and the effects of different dosages of which on the dissolution of su?cralfates were compared.RESULT:The sodium lauryl sulphate had the biggest promotion effect on the dissolution of sucral?fate.Optimal indexes of the capsule were achieved when the addition of sodium lauryl sulphate was2%.CONCLUSION:It is advisable to adopt2%sodium lauryl sulphate as the surfactants for the sucralfate capsule.

Objective To investigate the effect of pioglitazone on oxazolone-induced colitis and to clarify the mechanism of apoptosis by Fas/Fas ligand(FasL). Methods Eighteen BALb/c mice of ulcerative colitis induced by oxazolone enema were equally allocated into 3 groups. The mice in group 1 were sacrificed 3 days after enema,lamina propria mononuclear cells(LPMC) were isolated from freshly obtained colonic specimens and treated in vitro with pioglitazone (40 ?mol/L),the annexin-V-FITC was used for detection of apoptosis,and Fas/FasL expression was assayed by flow cytometry. Meanwhile,untreated mice were served as normal controls. Mice in group 2 (control group) and 3 (experiment group) were treated with methylcellulose or pioglitazone(20 mg?kg -1 ?d -1 ) 3 days after enema and were administrated for consecutive 7 days. The colonic inflammation including disease activity index (DAI),macroscopic and histological changes,myeloperoxidase(MPO) activity and levels of interleukin (IL)-4,IL-5 were evaluated. Results Apoptosis of LPMC,expression of Fas and FasL in normal and colitis mucosa were 12.89?1.23,70.63?6.24,8.59?5.47 and 4.25?0.84,62.60?5.85,23.75?10.23,respectively. After treated with pioglitazone,both apoptosis of LPMC and expression of Fas increased,while FasL expression decreased (40.58?10.32,83.98?11.38 and 10.04?5.21 respectively). In group 2 and group 3,the macroscopic and microscopic score,MPO activity,IL-4 and IL-5 level were 2.50?0.55,8.83?0.75, 3.81?0.17,216.46?34.32,102.28?25.74 and 0.33 ?0.52,4.00?0.63,1.25?0.16,179.36?18.15,61.65?17.45,respectively. Conclusion The results indicate that pioglitazone treatment can significantly attenuate colonic inflammation,and it might be related to the apoptosis of LPMC through Fas and FasL.

Objective: To construct and characterize GFP-mfgl 2 fusion protein expression plasmid (pEGFP-mfgl 2) and provide a direct and simplified methodology for primary assessment of the effect of mfgl 2 siRNA on the mfgl 2 gene expression. Methods: mfgl 2 cDNA was amplified from the mfgl 2 cDNA library pBluescript-m166 (pm166) of mouse genomic P1 plasmid and recloned into pEGFP-N2 upstream of GFP gene. The pEGFP-mfgl 2 was analyzed by restriction endonucleases BamH I and Hind III to ensure the orientation and the sequence. This fusion plasmid was then transfected into CHO cells and the fusion protein expression was observed by fluorescent microscope. Rusults: A 1.3 kb long cDNA was obtained. Restriction endonucleases and sequencing assays showed the correct orientation and sequence. After 24-48 hours transfection in CHO cells, the expression of pEGFP-mfgl 2 can be visualized through fluorescent microscope. Conclusion: pEGFP mfgl 2 has been constructed successfully. The recombinant vector can express GFP-mfgl 2 fusion protein. It provides a direct and simplified methodology for primary assessment of the effect of mfgl 2 siRNA on the mfgl 2 gene expression.