1.Advances in the Research of Chlorogenic Acid and Its Extraction and Isolation Methods
Chinese Traditional Patent Medicine 1992;0(02):-
The structure, pharmaceutical functions and extraction and isolation methods of chlorgenic acid were reviewed to provide references for the research of chlorogenic acids and the production of the medicines chlorogenic acids.
2.Determination of imperatorin and isoimperatorin in Tongketing Capsules by HPLC
Kai MA ; Wenjie QIN ;
Chinese Traditional Patent Medicine 1992;0(10):-
Objective: To establish the determination of imperatorin and isoimperatorin in Tongketing Capsule (Radix Angelicae Dahuricae, Pheretima, etc.) by HPLC. Methods: The determination was carried out by HPLC. The mobile phase was acetonitrile water (60∶40) and UV detection wavelength was set at 245nm . The flow rate was 1.0mL?min -1 . The column temperature was 40℃ . Results: There were the good linear relationship between the absorption area value and the concentration in the range of 65?g~325?g for imperatorin and in the range of 50?g~250?g for isoimperatorin, respectively. The average recoveries were 98.3% ( RSD = 1.73% ) and 98.7% ( RSD = 1.65% ), respectively. Conclusion: The method is simple, accurate and can be used to determine imperatorin and isoimperatorin in Tongketing Capsule.
3.Investigation on Construction Situation of Pharmacy Staff Team in 22 Hospitals from Hubei Wuling Moun-tainous Area
China Pharmacy 2015;26(31):4462-4464
OBJECTIVE:To investigate the status quo and problems of the construction of pharmacy staff team from Hubei Wuling mountainous area,and to provide reference for the formulation of construction plan of pharmacy staff team. METHODS:Using questionnaire survey and telephone interviews,the pharmacy staff of 22 hospitals at second level or above from Hubei Wul-ing mountainous area during 2011-2014 were investigated in terms of number,age,educational background,professional title,etc. RESULTS & CONCLUSIONS:The shortage of pharmacy staff is seious in 22 hospitals at second level or above from this areas (open 10 606 beds,should be staffed 956 pharmacy personnel,actual number of pharmacy personnel is 502);the age structure is unreasonable,and only 220 pharmacy staff aged under 35 year-old;highly educated pharmacy staff accounted for low proportion, and bachelor degree or above took up 29.5%;pharmacy staff with professional title took up low proportion,and pharmacy staff with high,middle and junior professional titles accounted for 1:5:4. The situation of pharmacy staff in the region can be improved through following measures,including striving for policy recommendations,continuously enriching the quantity of pharmacy staff, integrating scientifically and building reasonable talent tem gradually,introducing talents,optimizing education structure of pharma-cy staff,strengthening the training,improving operation level of pharmacy staff,etc.
4.Correlation research between Gene Polymorphism of TNF-alpha Gene 863C_A and Thyroid Associated Ophtalmopathy in Zhuang Ethnic Population
Chinese Journal of Primary Medicine and Pharmacy 2013;20(1):18-20
Objective To explore correlation between gene polymorphism of TNF-alpha and thyroid associated ophtalmopa thy (TAO) in Zhuang ethnic population.Methods DNA and serum samples were collected in patients with TAO in Han/Zhuang population.ELISA was applied to detect level of TNF-alpha in serum and PCR was applied to detect polymorphism of TNF-alpha gene 863C_A.Results The levels of TNF-alpha in patients with TAO increased significantly compared with control group (P < 0.05),and more significantly in Zhuang population compared with Han population (P < 0.05) ;gene frequency of CA was significantly higher in patients with TAO in Zhuang population than that in Han population (P < 0.05),while gene frequency of AA was significantly lower (x2 =0.770,2.145,all P < 0.05).Conclusion Serum level of TNF-alpha increases during occurrence of TAO,and the differences of expression level between different nationalities are due to gene polymorphism of TNF-alpha.
5.Advances in research on enterovirus 71 vaccines
Jie MA ; Yongqiang DENG ; Chengfeng QIN ; Ede QIN
Military Medical Sciences 2015;(7):553-556
Enterovirus 71 (EV71) is one of the primary causative agents of hand, foot and mouth disease in children and closely associated with severe neurological complications and even deaths.EV71 outbreaks have occurred throughout the Asia-Pacific region since 1990s, posing global public health threat;however, no specific therapeutic strategy exists for severe EV71 infection.Several inactivated vaccine products have entered or finished the clinical trial stage, and some novel vaccine candidates, including live attenuated, subunit, and virus-like particle, show great potential for further develop-ment.This review summarizes the present situation and progress in the development of EV71 vaccines.
6.Roles of TGF-?_1 and singal protein SMADs in rat myocardial hypertrophy
Jun HUANG ; Guohui QIN ; Yexin MA
Chinese Journal of Pathophysiology 1999;0(09):-
AIM: To investigate the role of SMADs singal pathway in rat myocardial hypertrophy. METHODS: The rat model of myocardial hypertrophy was produced by constriction of the abdominal aorta. The wet left vertricular/body weight ratio (LVMI) was measured. The expression of TGF-beta l and Smad 2, 3, 7 mRNA were assessed by RT-PCR. RESULTS: The LVMI and the expression of TGF-beta l and Smad 2, 3, 7 mRNA in cardiomyothy were increased in 3 day after the operation and continued at last 4 weeks. The peak expression of TGF-beta l and Smad 2, 3, 7 mRNA was in 2 weeks after operation. The expression of Smad 7 was increased in 3 days after operation, but the peak was in 1 week after operation, then decreased. CONCLUSION: The signal protein Smad 2, 3, 7 are involved in the progress of rat myocardial hypertrophy produced by constriction of abdominal aorta. [
7.A case report of laryngeal atypical carcinoid with multiple metastasis.
Hongfeng MA ; Yong QIN ; Wanmin CHENG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(8):575-576
The case of a 49-years-old man complained of pharyngalgia for one year and shortness of breath after activities for one week. Endoscopic laryngeal examination and computed tomography revealed a supraglottic mass. Direct laryngoscopy was performed and biopsy of the mass was carried out. Results of the histopathologic examination and immunohistochemical analysis were consistent with atypical carcinoid tumor of the larynx.
Carcinoid Tumor
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pathology
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Humans
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Laryngeal Neoplasms
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pathology
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Male
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Middle Aged
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Neoplasm Metastasis
8.On Improving Effects on Military Hygiene Teaching in Nursing Specialty
Wenling MA ; Zhengyin LI ; Haihong QIN
Chinese Journal of Medical Education Research 2006;0(09):-
Several problems were existed in present military hygiene teaching.In recent years,integrated reforms were done about the improving undergraduate and teacher's activities,strictly choosing teaching contents,doing well extra curriculum,and serious examination in nursing teaching.Furthermore,the new presumptions were also discussed.
9.Establishing a Characteristic High-level Course Standard of Nursing Specialty in Military Hygiene
Wenling MA ; Zhengyin LI ; Haihong QIN
Chinese Journal of Medical Education Research 2002;0(01):-
The new course standard of nursing specialty in military hygiene was established against the main problems in military hygiene teaching and considering the peacetime and wartime need of nursing post.In this paper,guiding ideology,content choosing and the request of operational skill of this standard were discussed in detail.
10.Research progress of induced pluripotent stem cells and its bottleneck
Chinese Journal of Tissue Engineering Research 2014;(45):7358-7363
BACKGROUND:With the development of the research, induced pluripotent stem cel s are applied to the build of disease model, drug screening, regenerative medicine, and many other research fields, and have made significant achievements, especial y in the study of nervous system diseases.
OBJECTIVE:To summarize the recent development of induced pluripotent stem cel s and to raise problems and prospects based on the latest research in this field.
METHODS:The first author searched the PubMed database for articles about the induced pluripotent stem cel s, including reviews, clinical research and basic research, published from January 2006 to September 2014. The keywords were“iPS, induced pluripotent stem cel”, and final y 60 articles were included in result analysis.
RESULTS AND CONCLUSION:Induced pluripotent stem cel research continues to make breakthrough from its discovery by Yamanaka’s team in 2006 to winning Nobel Prize in 2012. Induced pluripotent stem cel research has broad prospects in the disease model construction, drug screening and regenerative medicine. Currently, problems such as reprogramming methods, cel stability, and clinical transformation stil need to be solved, and further researches are necessary.