Objective To investigate the possible biochemical mechanism of cognitive impairment in patients with Obstructive sleep apnea syndrome(OSAS).Methods Polysomnography was administered to eighteen patients with OSAS and fourteen education and BMI-matched controls.All the cases were measured serum nitric oxide(NO)level by spectrophotometry and underwent neuropsychologicol test.Results The scores of visual recognition and digital symbol in the patients with OSAS were respectively 5.33?1.57;6.89?1.23,significantly lower than that in control group(8.57?1.91;9.07?1.00,P

OBJECTIVE:To investigate inhibitory effects of matrine on the proliferation of human bladder cancer BIU-87 cells and its mechanism. METHODS:The cell viability was detected by MTT assay and inhibitory rate of cell proliferation was calculat-ed after human bladder cancer BIU-87 cells were treated with 0(negative control),0.5,1.0,2.0 and 4.0 mg/ml matrine for 24,48 and 72 h,respectively. After treated with 0 (negative control),0.5,1.0,2.0 and 4.0 mg/ml matrine for 48 h,the cell cycle and apoptotic rate were detected by flow cytometry;the expression of Survivin,Caspase-3 and Caspase-7 protein were detected by Western blot assay. RESULTS:Compared with negative control,the proliferation of BIU-87 cells were significantly inhibited after incubated with 1.0-4.0 mg/ml matrine for 24,48 and 72 h(P<0.05 or P<0.01),and inhibitory rate of cell proliferation increased in concentration and time-dependant manner;after treated for 48 h,the percentage of G0/G1 phase cells and apoptotic rate in-creased,while the percentage of cells at S phase and G2/M phase were decreased (P<0.05 or P<0.01);the expression of Cas-pase-3 and Caspase-7 protein increased,while the expression of survivin protein decreased after incubated with 0.5-4.0 mg/ml ma-trine for 48 h. CONCLUSIONS:Matrine can inhibit the proliferation of human bladder cancer BIU-87 cells,block the cell cycle and induce apoptosis;its mechanism may be related to the expression regulation of Survivin,Caspase-3 and Caspase-7.

Objective To investigate the method and influencing factors of laser treatment in the removal of complicated tattoos.Methods 1280 patients with tattoos were treated by Versapulse four wavelength laser system.According to different colors,we selected different energy parameters and wavelength.Results All the 1280 patients got satisfied results after the therapy. The response was mainly related with the composition of their tattoos' color, as well as the proper choosing of laser wavelength and parameter.Conclusions According to feature of different tattoos,selecting reasonable wavelength and therapeutic parameters to treat the complicated tattoos can obtain better efficacy.

Objective:To optimize the water extraction process of Lidan Palshi capsules. Methods:The extract yield, emodin con-tent and chrysophanol content were used as the indices, the extraction process of Lidan Palshi capsules was optimized by orthogonal test of L9(34). Results:The optimized water extraction conditions were as follows:adding 14-fold water, and boiling extraction four times with 2 hours for each time. Conclusion:The water extraction process is practicable, reasonable and convenient.

Objective:To study the quality standard of Ganyankang capsules. Methods:Rhizoma Polygoni Cuspidati,Radix As-tragali,Radix Pseudostellariae,Herba Scutellariae Barbatae,Oldenlandia diffusa willd,Radix Salviae Miltiorrhizae and Radix Bupleuri in the preapartion were indentified by TLC,and emodin was determined by HPLC. Results:Rhizoma Polygoni Cuspidati,Radix Astra-gali,Radix Pseudostellariae,Herba Scutellariae Barbatae,Radix Salviae Miltiorrhizae and Radix Bupleuri could be obviously identified by TLC. The content of emodin in the capsules was 2. 05 mg·g-1 . The linear relationship was good within the range of 2. 34-74. 88μg·ml-1(r=0. 999 4),and the average recovery was 100. 5% with RSD of 1. 52%(n=9). Conclusion:The quality standard is accurate and reliable,which can be used to control the quality of Ganyankang capsules.

Objective:To optimize the ultrasonic extraction process of Danggui Baogan capsules. Methods:The ultrasonic extrac-tion process of Danggui Baogan capsules was optimized by L9 (34 ) orthogonal test with the contents of ferulic acid and angelica sinensis polysaccharide as the indices and the amount of 30% ethanol, ultrasonic duration and extraction times as the influencing factors. Re-sults:The best ultrasonic extraction conditions were as follows: adding 12-fold amount of 30% ethanol and extracted four times with 1. 5 hours for each time. Conclusion:The ultrasonic extraction process is simple and reproducible, and suitable for the industrial pro-duction.

Objective To investigate effects of Kanglaite injection on proliferation, cycle and apoptosis of human breast cancer MCF-7 cells;To discuss its relevant mechanism. Methods Logarithmic growth phase cells were divided into control group and Kanglaite-treatment group (10, 20, 40μL/mL). Cells were cultured in RPMI-1640 for 24 h before drug treatment. The inhibition rate of Kanglaite injection on proliferation of human breast cancer MCF-7 cells was detected by MTT assay. Apoptosis and cell cycle of MCF-7 cells were detected by flow cytometry. Changes in cell nucleus were determined by Hochest staining assay. Protein expressions of Bcl-2 and Bax were detected by ELISA and Western blot. Results Kanglaite injection for 12 h, 24 h or 48 h resulted in a significant inhibition of MCF-7 cells proliferation (P<0.05, P<0.01);Compared with the control group, Kanglaite injection-treated cells showed increased percentage in G2/M and G0/G1 phases (P<0.001, P<0.01), but showed decreased percentage in S phase (P<0.01), and apoptosis rate increased (P<0.05, P<0.001). Kanglaite injection significantly decreased protein expression of Bcl-2, and enhanced protein expression of Bax of MCF-7 cells (P<0.01, P<0.001). Conclusion Kanglaite injection can inhibit the proliferation of human breast cancer MCF-7 cells, decrease cell cycle and induce apoptosis, the mechanism is related with decreasing protein expression of Bcl-2 and enhance the protein expression of Bax.

A 2-month-old baby girl developed universal keratotic plaques soon after birth. Physical examination revealed well-defined, dark erythematous, keratotic plaques with thick scales and mild infiltration at the periorbital, perioral, perianal and vulvar regions, as well as deep fissures of both hands and feet covered with thick yellowish crusts. Another case was a 24-year-old female, the mother of the baby, who presented with hyperkeratotic plaques at perioral and perianal regions, congenital alopecia universalis, mutilation of fingers and toes with massive thick keratotic yellow crusts and scales. Histopathology of skin lesions from the gluteal region of the baby showed psoriasiform hyperplasia of the epidermis, slight inflammatory infiltration of dermal papillae and superficial dermal perivascular regions. Immunohistochemistry demonstrated the positive staining for acidic keratin (AE1) in the prickle cell layer and granular layer and for CK10 in the upper prickle cell layer and granular layer. Electron microscopy showed increased cell space and decreased tonofilament. Both the baby girl and her mother were diagnosed with Olmsted syndrome.

Vaccine immunization represents the most effective strategy against viral infections .Antiviral vaccine candi-dates currently include live attenuated vaccine , inactivated vaccine , DNA vaccine and genetic engineering vaccine .Live at-tenuated vaccines , eliciting efficient humoral and cellular immune response by simulating natural virus infection , are clini-cally widely used.Traditionally, live attenuated vaccine strains are obtained by serial passaging in vitro or in vivo, a costly procedure accumulating random mutations .microRNA( miRNA)-targeting host gene regulation exhibits high species and tis-sue specificity .Introducing certain miRNA target sequence into viral genome by reverse genetic technique can effectively attenuate virus strains, which has great potential in vaccine design and development .In this article,attenuation strategies and progress in its application in vaccine research are disscussed .

Objective To observe the effects of Jieyu-Chufan capsule(JCC) on the behavior and the monoamine neurotransmitter content of cerebral in depression mouse model and explore its mechanism. Methods The ICR male mouse were randomly divided into seven groups according to their weights(the model group, the low, middle and high doses group, Baiyoujie group, the amitriptyline group, the normal control group). The low, middle and high doses group were given JCC with 1.250, 2.500, 5.000 g/kg separately by intragastric administration. Baiyoujie group was given 0.014 g/kg Baiyoujie by gavage. The amitriptyline group was given 0.050 g/kg amitriptyline by gavage. The model group and the normal control group received the same volume of distilled water intragastrically. The drugs were administrated once a day. The brepharoptosis and movement conditions of mouse were observed after 22 days. The monoamine neurotransmitter content of each group was measured after 25 days. Results Compared to the model group, the number of mouse with brepharoptosis decreased in the high dose group after 1, 2, 6 hour with reserpine injection (P<0.05 or P<0.01);the number of mouse came out from the circle increased after 4 hours with reserpine injection (P<0.05 or P<0.01);the content of NA (880.45 ± 428.81 ng/g, 875.98 ± 449.33 ng/g vs. 299.92 ± 267.08 ng/g) and DA (2 305.99 ± 530.37 ng/g, 2 169.99 ± 278.19 ng/g vs. 1 439.34 ± 357.33 ng/g) in the middle and high dose group increased (P<0.01);the content of 5-HT (781.43 ± 135.10 ng/g vs. 492.01 ± 192.80 ng/g) in the middle group increased (P<0.01). Conclusions JCC showed an antidepression influence in depression mouse model induced by reserpine. The mechanism may be related to regulating the level of monoamine neurotransmitter in the nervous centralis .