1.Effects of intervening measures on postpartum depression
Fengsen XU ; Jianxin LIU ; Shuping ZHANG ; Juan LI ; Qin SU
Chinese Journal of Obstetrics and Gynecology 2000;0(12):-
Objective To study the related factors of postpartum depression (PPD) and the effects of intervening measures to PPD incidence. Methods 1 597 pregnant women selected from our antenatal care clinic were investigated by using the hospital anxiety and depression questionnaire (HAD) during pregnancy and the Edinburgh postpartum depression scale (EPDS) after childbirth.All the enrolled women were randomlyd divided into control group and intervening group by the proportion of 1 to 2.Six intervening measures were used in the latter group. Results (1) There were 49 women whose HAD≥11 score (anxiety-depression mood)with 28 cases (57.1%) had got postpartum depression in the control group.In the intervening group,however,there were 94 women whose HAD≥11 score with 24 cases (25.5%) had got postpartum depression.There is a significant difference between the two groups ( P
2.Establishment of a 15 loci multiplex amplification system and the genetic poly- morphism in Xinjiang Uygur population.
Juan GUI ; Hai-Bo LIU ; Qin-Xiang LIAO ; Xu XU ; Di LU ; Li YUAN
Journal of Forensic Medicine 2015;31(1):23-27
OBJECTIVE:
To develop a five fluorescence-labeled multiplex amplification system for 15 loci and study genetic polymorphism in Xinjiang Uygur population.
METHODS:
The STR loci were screened. The alleles were named according to the number of repeats by sequencing. The sensitivity, species specificity, identity and stability of the five fluorescence-labeled multiplex amplification system for the 15 loci were all tested. Then, the genetic polymorphism was analyzed in Xinjiang Uygur population and compared with other ethnic groups including Xizang Tibetan, Xiuyan Manchu, and Guangzhou Han population.
RESULTS:
The 15 loci multiplex amplification system was established. The sensitivity was 0.3 ng with good species specificity, identity and stability. The distributions of genotype for 13 STR loci in Uygur population were in accordance with Hardy-Weinberg equilibrium with no genetic linkage between these loci. Most loci showed statistically significant among different populations.
CONCLUSION
The established system has application value in forensic evidence. The 13 STR loci in Uygur population have
Alleles
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Ethnicity/genetics*
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Gene Frequency
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Genetic Linkage
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Genotype
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Humans
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Multiplex Polymerase Chain Reaction/methods*
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Polymorphism, Genetic
3.Analysis on signaling pathway network of proliferation of neural stem cells.
Qing-Shan LIU ; Shu-Juan ZHUANG ; Ke-Qin LI ; Xu LI
China Journal of Chinese Materia Medica 2014;39(3):407-411
Neural stem cells in brains have capacities of proliferation and differentiation, which is very critical to rebuild the cerebral cortex functions. Therefore, it is of great importance to find key targets and network pathways that regulate the proliferation of neural stem cells, which is also a pressing problem in the medical circle. With the Notch pathway as the core of the network, this paper summarized the advance of the bimolecular network system composed of Wnt, Shh, EGFR, cytokines and Notch signal, and analyzed such key nodes as Notch receptor, CBF1, NICD, Hesl, which may become potential targets of new-type drugs in the future. With the multi-component, multi-target, multi-lever characteristics, traditional Chinese medicines have many common grounds with the network pharmacology. The active component groups or active ingredients in traditional Chinese medicines are one of the material bases for showing their network pharmacological effect, which is worth exploring. This paper aims to provide a new strategy for the treatment of neurodegenerative disease and nerve injury with traditional Chinese medicines.
Animals
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Cell Proliferation
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Humans
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Neural Stem Cells
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cytology
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metabolism
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Signal Transduction
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Systems Biology
4.A retrospective analysis of high risk factors for nonunion of femoral neck fractures
Bin XU ; Yueju LIU ; Zhiyong LI ; Qi ZHANG ; Juan WANG ; Shiji QIN ; Zhaoyu CHEN ; Yingze ZHANG
Chinese Journal of Trauma 2012;(12):1083-1087
Objective To retrospectively study postoperative nonunion of femoral neck fractures so as to identify high risk factors for their nonunion.Methods A total of 965 patients with complete clinical data out of the 1 323 patients with femoral neck fractures treated by internal fixation with cannulated compression screws from January 2003 to January 2011 were included in the study.Logistic muhiple regression method was used to analyze five factors including patients' age,gender,injury mechanism (whether high energy injury or not),fracture type (with or without displacement) and medical comorbidities [American Society of Anesthesiologists Physical Score (ASAS) grade] and their correlation with fracture healing to investigate the high risk factors for the nonunion of femoral neck fractures.Results Quantitative assay of the factors affecting healing of femoral neck fractures was ranged as follows:medical comorbidities (ASAS grade),injury mechanism (whether high energy injury or not),fracturc type (with or without displacement),gender and age.Conclusion Age > 50 years,females,displacement fracture,high energy injury,and ASAS grade above Ⅲ are high risk factors for nonunion of femoral neck fractures.
5.High-risk human papillomaviral infection characterized by pseudocondyloma of vulvae
Juan XU ; Jing WANG ; Xuedong LU ; Qin HE ; Jiayuan CHEN ; Li XIANG ; Xiameng HUANG
Chinese Journal of Dermatology 2009;42(5):303-304
Objective To investigate the clinical features and human papillomavims (HPV) types in 12 cases of HPV infection characterized by pseudocondyloma of vulvae. Methods Twelve women, who had a history of sexual behavior with non-spouses and presented with pseudocondyloma of vulvae, were included in this study. Tissue specimens were scraped or resected from the lesions of pseudocondyloma of vulvae. PCR-based reverse hybridization assay was performed to determine the genotypes of HPV. Results All the 12 specimens were HPV positive. The reverse hybridization assay generated 13 subgenotypes of HPV, including 3 low-risk genotypes (11, 42 and 43) and 10 high-risk genotypes (16, 18, 31, 33, 35, 39, 51, 52, 58 and 59). All the patients were found to be infected by at least one high-risk genotype. Of the 12 patients, 4 were infected by single type of HPV, 6 by 2 types of HPV, and 2 by 3 or 4 genotypes of HPV. Conclusion The patients characterized by pseudocondyloma of vulvae may suffer from high-risk HPV infection.
6.Advances in Mechanism of Microbial Degradation of Phenanthrene
Jing WANG ; Hong-Ke XU ; Yuan-Qin LIU ; Su-Juan LI ;
Microbiology 1992;0(05):-
This review is outlined in terms of the advances in mechanism about microbial degradation of phenanthrene. The degradation pathways of phenanthrene by bacteria and fungus , including aerobic and anaerobic conditions, are discussed respectively. Furthermore, both the enzymes involved in the reactions and the gene clusters encoding for the enzymes are summarized. The application of gene probe is introduced briefly. Based on the preliminary results of our laboratory, it is found that some questions should be taken into more consideration.
7.1483 cases of paternity test with STR loci mutation.
Li SHUAI ; Jun WANG ; Qiang JING ; Bin XU ; Qin SU ; Yun-Juan LI ; Hui LI
Journal of Forensic Medicine 2014;30(1):44-46
OBJECTIVE:
To observe and analyze the mutation phenomenon of 17 STR loci of PowerPlex 18D Kit in paternity test of Yunnan population.
METHODS:
The DNA was extracted by Chelex-100 method. The PowerPlex 18D Kit was used to test 1,483 cases and their conclusions of paternity tests were verified.
RESULTS:
In the 1,483 cases, 1,047 were parental triplet and 436 were uniparental diad. A total of 2,530 times of meiosis was observed. One STR locus mutation was observed in 24 cases. And 11 mutation loci were found in the 17 STR loci.
CONCLUSION
STR loci mutation is a common phenomenon. We should collect the data of STR loci mutation, choose other good polymorphism, low mutation rate of genetic markers, to ensure that the results are accurate and reliable.
China
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DNA
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DNA Fingerprinting
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Female
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Gene Frequency
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Genetic Markers
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Humans
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Microsatellite Repeats
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Mutation
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Paternity
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Polymorphism, Genetic
8.Infrared charts of three groups of winter swimming, normal constitution and HIV/AIDS analysed by ATA technique
Hong-Juan LI ; Jian WANG ; Wen ZOU ; Hui-Juan GUO ; Lei HOU ; Jun-Qin XU ; Ying LIU ; Lei ZHOU
Journal of Beijing University of Traditional Chinese Medicine 2010;33(2):94-97
Objective To study the infrared charts of three groups of winter swimming, normal constitution and HIV/AIDS, explore the characteristics of the thermal difference (△t) data of automatic thermal analysis (ATA) for reviewing the conditions of human healthy qi by applying ATA technique, and analyse the conclusion combined with TCM theory.Methods The cross-section research of the three groups was designed for observing △t data of Governor Vessel (GV), Conception Vessel (CV), Shenque (CV8), kidney, life gate, triple energizer, five zang-viscera and six bowels. The data were computed mathematically by using single factor variance analysis.Results The relative △t of GV in the three groups showed a descending order, and pairwise comparison had statistical significance respectively (P<0.01). The relative △t of CV8 and life gate was higher significantly in the groups of normal constitution and swimming than that in the group of HIV/AIDS, and △t of CV was higher significantly in the group of HIV/AIDS than that in the groups of normal constitution and swimming. The relative △t of kidney in the three groups showed a descending order, and pairwise comparison had statistical significance respectively (P<0.01).Conclusion The changes of △t of GV, CV, CV8, kidney, life gate, triple energizer, five zang-viscera and six bowels express the conditions of human healthy qi in this experiment, which is in accordance with the explanation of healthy qi in TCM theory.
9.Relationship between viral genotype and specific and nonspecific CTL of patients with cirrhotic hepatitis B and its significance
Xi-Bing GU ; Xiao-Juan YANG ; Juan-Hua WANG ; Zhong HUA ; Zhong-Hua LU ; Yue-Qin XU ; Zhi-Yuan JIA
Chinese Journal of Experimental and Clinical Virology 2011;25(3):211-213
Objective To explore relationship between different HBV genotypes and peripheral blood HBV specific and nonspecific CTL of patients with cirrhotic hepatitis B and its significance. Methods HBV genotypes were tested in 91 patients with cirrhotic hepatitis B, differences of HBV specific and nonspecific CTL between patients infected with genotype B and C were compared and its significance was explored. Results In 91 cases of cirrhotic hepatitis B, 55 cases (60.44% ) belong to genotype C, 35 cases (38. 46% ) belong to genotype B, 1 case (1. 1% ) belongs to mixture genotype B and C. In genotype C,27 cases (49.09% ) had positive (HLA)-A2, HBV specific CTL was 0. 18% ±0.03%. In genotype B, 18 cases (51.43% ) had positive HLA-A2, HBV specific CTL was 0. 38% ± 0.04% , higher than that in genotype C,t =5. 01, P <0. 01. Nonspecific CTL: genotype C (11. 87% ± 1. 50% ) ; genotype B( 11. 90%± 1. 51% ), t =0. 14, P <0. 05. HBV DNA level; genotype C (6. 01 ± 0. 81) log10 copy/ml, higher than that in genotype B (5.01 ± 0.54) log10 copy/ml, t =5.01, P <0.01. ALT; genotype C (251. 13 ± 131. 11) U/L, higher than that in genotype B (121. 25 ± 63. 21) U/L, t =3. 61, P <0. 01. TBil (45. 61± 15.11) μmol/L, higher than that in genotype B (28.11 ±6.25) μmol/L, t = 3.05, P < 0.01. Conclusion Compared with patients infected with genotype B of cirrhotic hepatitis B, HBV specific CTL of patients infected with genotype C was lower, resulting in higher level of HBV DNA and more severe damage of liver function.
10.Surveillance and early diagnosis of small hepatocellular carcinoma with contrast-enhanced ultrasonography
Mei YU ; Qi MEI ; Juan NI ; Chen QIN ; Xiangyong XU ; Qingyin FU ; Hongfang SHA ; Lingyun ZHAI ; Gang WANG ; Jing ZHANG
China Oncology 2014;(3):203-207
Background and purpose:The incidence of liver cancer is high in China. Primary liver cancers usually occur in patients with liver cirrhosis, which is a challenge for the early diagnosis of liver cancer. Our purpose is to investigate the efifcacy of contrast-enhanced ultrasonography (CEUS) in the early identiifcation and diagnosis of small hepatocellular carcinoma (HCC) by regularly tracking and supervising the high risk population. Methods:A total of 320 high risk patients of HCC admitted in our hospital from February 2011 to November 2013 were enrolled in this prospective study. All patients underwent conventional ultrasound and hepatic CEUS. The differential diagnosis of malignant HCCs from benign ones was based on the enhancement patterns of hepatic lesions in different phases on CEUS. Results:Twenty patients were diagnosed as small HCC among 320 HCC high risk patients who were under regular surveillance using CEUS and all were pathologically conifrmed. Seven of the 20 HCC cases were smaller than 1.0 cm and 13 measured 1.1-2.0 cm. There were 6 (30.0%) HCCs presented as“early wash-in and slow wash-out”atypical pattern of HCC. The small size of the lesion and iso-echogenicity were the main factors of atypical pattern of HCC on CEUS.Conclusion:Ultrasonography and CEUS surveillance is a useful strategy for the early detection of small HCCs in high risk patients, which can help them to receive proper therapeutic management in time.