1.The diagnosis and surgical treatment of malignant gastrointestinal tumors in children
Huijuan WANG ; Huanmin WANG ; Hong QIN
Chinese Journal of Digestion 2009;29(10):666-669
Objective To investigate the essential points of the diagnosis and the surgical treatment of malignant gastrointestinal tumors in children.Methods Clinical data involved the sites and pathological types of the tumors,clinical manifestation,effective treatment and prognosis were retrospectively analyzed in 29 consecutive patients reffered to Beijing Children's Hospital between January 1996 and May 2008.Results Of 29 patients,26 were males and 3 were females(aged from 1 year and 10 month to 14 years,mean age of 10 years).Gastric tumor was found in 5 cases (interstitialoma in 1,lymphoma in 2,endodermal sinus tumor in 1 and P-J syndrome canceration in 1),whereas intestinal tumor was found in 24 cases(malignant Iymphoma in 17,colon carcinoma in 4 and carcinoid of appendix in 3).The main clinical manifestations were abdominal pain,emesia,fever,alimentary tract hemorrhage and emaciation.Surgical complication involved perforation,internal fistula,obstruction,invaginate and hemorrhage.Twenty-four patients underwent(gaster)intestine resection and anastomosis.Biopsy was performed in 5 patients.Two patients with colonic carcinoma relapsed 6 months to 1 year after operation.Of 29 patients,17 patients survived,6 patients missed the follow-up and 6 patients died.Conclusions Pediatric malignant gastrointestinal tumor is rare.The unspecific presentation makes the diagnosis difficult.In diagnosis of acute abdomen and gastrointestinal tract diseases,the possibility of malignant tumor should be reminded.The imaging and endoscopic examination are very important to diagnosis.The surgical resection or combined with chemotherapy are mainly used in treatment of tumors.The prognosis is related to the pathological nature of the tumor.
2.27-O-(E)-p-coumaric acyl ursolic acid via JNK/SAPK signal pathway regulates apoptosis of human breast cancer MDA-MB-231 cell line.
China Journal of Chinese Materia Medica 2015;40(4):722-726
27-O-(E)-p-coumaric acyl ursolic acid( DY-17) from Ilex latifolia is a compound of the monomer. To investigate the DY-17 inducing apoptosis in the human breast cancer cell line, the MDA-MB-231 cells were used as research object in this experiment. The proliferation activity of the MDA-MB-231 cells stimulated with the different concentrations of DY-17 (20, 40 µmol · L(-1)) was detected at different time( 12, 24, 36, 48, 60,72 h) . We surveyed the DY-17 inducing apoptosis of the MDA-MB-231 cells with the fluorescent staining technology. The rate of MDA-MB-231 cells apoptosis and necrosis was determined by flow cell cytometry (FCC). Moreover, expression of JNK, phosphorylated JNK, Bax, PARP shear and caspase-3 shear related to JNK/SAPK pathways were investigated in every group ( control group, EGF group, EGF + DY-17 40 µmol · L(1) group and EGF + SP600125 group) with Western blot. The MTT results showed that, in the presence of DY-17, the proliferation activity of MDA-MB-231 cells decreased in a dose-dependent and time-dependent manner. The apoptosis and necrosis rates of MDA-MB-231 cells with DY-17(20, 40 µmol · L(-1)) groups was respectively 31.86%, 49.91% by flow cytometry and significantly increased compared with control group under Fluores- cence microscopy. Up-regulation of the JNK phosphorylation protein expression was observed in EGF group compared with control group. In addition, markedly decreased the expression of JNK phosphorylation protein were also surveyed in EGF + DY-17 40 µmol · L(-1) group compared with EGF group. The expression of Bax, shear PARP and shear caspase-3 protein in EGF + DY-17 40 µmol · L(-1) group were significantly increased in comparison with EGF group. The results showed DY-17 induced apoptosis of human breast cancer MDA-MB-231 cell line related to down-regulating JNK/SAPK signal pathways.
Apoptosis
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drug effects
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Breast Neoplasms
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drug therapy
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enzymology
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genetics
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Cell Line, Tumor
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Cell Proliferation
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drug effects
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Drugs, Chinese Herbal
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pharmacology
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Female
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Humans
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MAP Kinase Kinase 4
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genetics
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metabolism
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Mitogen-Activated Protein Kinase 8
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genetics
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metabolism
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Signal Transduction
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drug effects
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Triterpenes
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chemistry
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pharmacology
3.Research on ECG Denoising during Spaceflight
Zhonggang LIANG ; Qin WANG ; Hong YAN
Space Medicine & Medical Engineering 2006;0(05):-
Objective To find a method for denoising of ECG signals during spaceflight.Method According to noise character of ECG during spaceflight,a new denoising method that combines wavelet transform and shape filter was put forward.Result By practical application and simulation test,it was proved that this method could eliminate efficiently noise from ECG during spaceflight.Conclusion The method that combines wavelet transform and shape filter can be used for ECG denoising during spaceflight.
4.Nucleotide sequence analysis of HCV NS5 region in Shandong province
Qin WANG ; Wenqing ZHANG ; Hong YU
Chinese Journal of Infectious Diseases 2001;0(06):-
Objective To study the genotypes and the variations of HCV isolates in some areas of Shandong province.Methods The kit of AKZO UBI EIA 4.0 assay for antibody to hepatitis C virus(HCV)was used to screen 64 clinical anti HCV Ab positive serum samples, and 54 samples of which gave positive results. With RT nested PCR that utilized highly efficient primers,a product of 319bp in the NS5 region was amplified from 12 of 28 samples.These 12 PCR products were directly cloned using TA cloning kit, and 10 independent clones were isolated and sequenced by dideoxynucleotide chain termination reaction. Ressults Every sequencing analysis followed by comparison with reported sequences in the GenBank revealed that 10 sequences were all classified as HCV type 1b. All nucleotides changes in NS5 region were due to substitution without insertion and deletion,and most of the substitution (74%)occurred in the third base of a codon; The motif of G D D and all cysteines were conserved. Conclusion The result has demonstrated that HCV 1b is predominant in some areas of Shandong province.
5.The application of PBL in evidence-based medicine teaching among international students
Qin LIU ; Hong WANG ; Zhaohui ZHONG
Chinese Journal of Medical Education Research 2011;10(9):1133-1135
PBL ( Problem-based learning) is a problem-based and student-centered learning pattern.PBL teaching method has been applied to evidence-based medicine ( EBM ) courses for international students.By this method,the students' learning enthusiasm was stimulated,the ability of ananlyzing and resolving problem was improved,and good teaching effect was achieved,which provided knowledge to improve further EBM teaching and evidence-based clinical practice.
6.Design and Implementation of Comprehensive Outpatient Cashier Management System Based on RFID
Jing SUN ; Hong WANG ; Xiangpeng QIN
Chinese Medical Equipment Journal 2003;0(12):-
Objective To enhance the quality and efficiency of the outpatient cashier service.Methods A comprehensive outpatient cashier management system based on RFID was developed and put into use.Results The system could be used in outpatient cashier department for attendance checking,schedule management,bonus distribution,personnel management,data statistics and report printing.Conclusion The comprehensive outpatient cashier management system can improve the efficiency of the outpatient department.
7.Surgical Treatment of Primary Retroperitoneal Tumor
Qian QIN ; Hong LI ; Libin WANG
Journal of Chinese Physician 2001;0(05):-
Objective To investigate diagnosis and surgical treatment of primary retroperitoneal tumor. Methods The clinical data of 26 cases with primary retroperitoneal tumor was retrospectively analyzed.Results 13 cases with benign tumor and 10 cases with malignant tumor underwent total resection, only 3 cases with malignant tumor underwent local resection. One case with malignant tumor recurred within 2 weeks and death after operation about 28 days.Conclusions IVP,B-ultrasonography, CT and MR diagnosis of primary retroperitoneal tumor is very important for preparation of preoperation and estimation of target organ. Repeatedly operation can improve cure rate.
8.Clinical Observation on the Efficacy of Three Different Reconstructive Methods of Alimentary Canal for Total Gastrectomy
Qian QIN ; Hong LI ; Libing WANG
Journal of Chinese Physician 2001;0(05):-
Objective To compare the clinical effects of different reconstructive modes of alimentary canal for total gastrectomy. Methods 67 cases of the patients with gastric malignant tumor received total gastrectomy and alimentary canal restruction of SS loop /Roux_Y, P loop/Roux_Y or Roux_Y. The diety complaint, digestive tract symptom and the nutritional status of the patients were investigated 6 months after operation. Results Compared with Roux_Y or Lahey+Broun mode,the complication frequency of SS loop /Roux_Y and P loop/Roux_Y modes was significantly lower(P
9.Early Diagnosis aed Treatment of Duodenal Trauma: A Report of 36 Cases
Qian QIN ; Hong LI ; Libing WANG
Journal of Chinese Physician 2001;0(08):-
Objective To explore the early diagnosis and operative treatment of duodenal injury. Methods The clinic data of 36 patients with duodenal trauma were retrospectively analyzed. Results Among 36 cases of duodenal injury, 4 cases (4/36,10.3%) were located in the first section of duodenum, 26 cases (26/36,72.2%) were between the second and third sections of duodenum, 6 cases (6/36,16.7%) were located in the fourth section of duodenum, and 14 cases were accompanied by other organ injury. The frequency of the postoperative complications was 13.9%, the curative rate was 94.4%, and the mortality was 2.8%. Conclusion Familiarizing with charateristics of duodenum injury, early diagnosing, mastering surgical exploration indication, selecting suitable operative approach, effective duodenal decompression, sufficient drainage and anti-infection treatment can improve the curative rate of duodenal injury.