1.Association Between Different ER Expression and the Sensitivity of TC Chemotherapy in Locally Advanced Breast Cancer Patients
Suhong SUN ; Qin HUANG ; Feng ZENG
China Pharmacy 2005;0(20):-
OBJECTIVE:To observe the association between the ER expression and the sensitivity of TC chemotherapy in locally advanced breast cancer patients.METHODS:The expression of ER in 57 locally advanced breast cancer patients was detected by EnVision method,and the sensitivity to TC chemotherapy in ER negative(-)vs.ER positive(+)patients were evaluated.RESULTS:There were significant differences between the ER negative(-)patients and the ER positive(+)patients in the sensitivity to TC chemotherapy(86.36%(19/22)vs.60.00%(21/35))(P
2.Anti-migratory and anti-invasive effect of somatostatin receptor type2 gene in human pancreatic carcinoma cell
Yanping FENG ; Jun GAO ; Tao HUANG ; Qin CHANG ; Renyi QIN
Chinese Journal of General Surgery 2000;0(11):-
Objective To investigate the anti-migratory and anti-invasive effect of somatostatin receptor type 2(SSTR2) gene transfection mediated by adenovirus in human pancreatic carcinoma cell and the mechanisms involved in this effect.Methods The full length human SSTR2 cDNA was introduced into pancreatic cancer cell line BXPC-3 by adenovirus-mediated transfection,and stable expression of RNA and protein of SSTR2 were detected by RT-PCR and Westen-blot.The Matrigel coated Transwell was used to detect the migratory and invasive ability of SSTR2expressing cells,Adv-GFP control cells and mock control cells.Furthermore,the expressions of matrix metalloproteinase-2(MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2) were detected by RT-PCR method in these cells.Results The stable expression of SSTR2 was detected in BXPC-3 cells transfected by Adv-GFP-SSTR2.A dramatic decrease of BXPC-3 expressing SSTR2 cell(migrated) through a Matrigel-coated filter was observed,as compared with Adv-GFP control cells and mock control cells(P
3.Metabolic response of different load of nutrition and recombinant human growthhormone after major abdominal surgery
Feng ZHU ; Xihu QIN ; Bohua HUANG ; Jian HUANG
Parenteral & Enteral Nutrition 1997;0(01):-
Objective:To investigate the metabolic response of different load of nutrition and recombinant human growth hormone(rhGH)after major abdominal surgery. Methods:32 patients were randomly divided into three groups as fellows:Group A : low calorie load,83.68 kJ/(kg?d);Group B: low calorie load combined with rhGH,83.68 kJ/(kg?d) and rhGH 4 U/d from d1-7 after operation;Group C :high calorie load combined with rhGH,125.52 kJ/(kg?d) and rhGH 4 U/d from d1-7 after operation.The levels of serum albumin and prealbumin and the excretion of urea nitrogen in urine were measured.after operation. Results:On day 8 after operation,the level of prealbumin in group B and C were higher than in group A(P
4.Thirty cases of knee osteoarthritis treated by acupuncture and warming needle moxibustion.
Guo-ping XIONG ; Qin HUANG ; Yuan-feng DONG
Chinese Acupuncture & Moxibustion 2011;31(6):551-552
Acupuncture Therapy
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Adult
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Aged
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Female
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Humans
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Male
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Middle Aged
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Moxibustion
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Osteoarthritis, Knee
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therapy
5.D-Penicillamine induced nephrotic syndrome and myasthenia gravis:two cases report and review of literature
Hui-Qin HAO ; Feng HUANG ; Wei ZHAO ; Qian-Xun ZHAI ;
Chinese Journal of Rheumatology 2003;0(11):-
Objective To improve clinicians'understanding of nephrotic syndrome and myasthenia gravis as adverse events of penicillamine(D-PA).Methods Two patients were reported to develope adverse reaction after taking D-PA.The related literature were reviewed.Result The first case was a systemic sclero- sis(SSc)patient treated with D-PA who developed nephrotic syndrome and blepharoptosis.The second case was a rheumatoid arthritis patient treated with D-PA who developed dysphagia and drinking bucking.The clinical symptoms were improved after disconting D-PA.Their conditions were stable up to now.Conclusion D-PA is used widely for various of autoimmune diseases.Clinicians should pay attention to these special and rare adverse effects.
6.Comparison of different doses of ephedrine combined with hydroxyethyl starch as a volume preload for preventing hypotension in caesarean section before combined spinal and epidural anesthesia
Weibo HUANG ; Feng WANG ; Jialing MO ; Chenjuan BAO ; Zhiren QIN
Chinese Journal of Primary Medicine and Pharmacy 2013;20(14):2089-2091
Objective To evaluate the effects of different doses of ephedrine combined with hydroxyethyl starch as a volume preload for preventing hypotension in caesarean section before combined spinal and epidural anesthesia.Methods 250 pregnant wemen undergoing cesarean section were randomly allocated into the control group (n =50) and the observation group(n =200)(group was divided into A,B,C,D group,each group 50 cases).Control group preloaded Lactated Ringer's solution 250ml before CSEA.A group preloaded hydroxyethyl starch combined with 5mg ephedrine; B group preloaded hydroxyethyl starch combined with 10mg ephedrine.C group preloaded hydroxyethyl starch combined with 15mg ephedrine; D group preloaded hydroxyethyl starch combined with 15mg ephedrine.The parturient SBP,HR and untoward reaction were monitored in five experimental groups.Results Compared with T0 A group parturient SBP was lower at T1,T2,T5 [(111.8 ± 17.18)mm Hg,(114.58 ± 19.80)mm Hg,(115.06 ± 10.39) mum Hg vs (120.88 ± 13.24) mm Hg,all P < 0.05)].Compared with B,C,D group,statistical differences were found at T1,T4,T6[(111.8 ± 17.18)mm Hg vs (120.78 ± 14.47)mm Hg,(118.56 ± 14.25)mm Hg,(118.42 ± 18.71)mm Hg.(125.58 ± 14.45) mm Hg vs (120.02 ±21.15)mm Hg,(115.92 ± 17.56)mm Hg,(119.00 ±12.49)mm Hg.(118.08 ±9.09)mm Hg vs (121.52 ± 10.92) mm Hg,(116.04 ± 11.61)mm Hg,(124.98 ± 9.16) mm Hg,all P < 0.05].Compared with T0 parturient HR was undulation at T1,T5 (P < 0.05).Compared with C,D group,statistical differences were found at T1,T3,T4,T6 [(82.92 ± 19.55) times/min vs (98.86 ±17.82)times/min,(96.72 ± 17.91) times/min.(89.04 ± 16.68) times/min vs (92.10 ± 16.55) times/min,(98.46 ± 19.49) times/min.(87.56 ± 17.13) times/min vs (98.86 ± 16.76) times/min,(88.58 ± 19.22) times/min.(93.20 ± 14.07) times/min vs (98.80 ± 11.69) times/min,(90.98 ± 10.93) times/min.all P < 0.05].In B and C group,parturient SBP and HR were steady and the untoward reaction was very few.Although parturient SBP of D group was steady,but HR obviously fast during operation (P < 0.05).Compared with B,C group,statistical differences were found at T4,T6 (P < 0.01).Conclusion The optimum dose is 10 ~ 15 mg of ephedrine.It combined with hydroxyethyl starch as a volume preload can keep the stable of blood circulation.
7.Application of Ligasure vessel sealing system for resection of retroperitoneal tumor
Junying YU ; Shunrong HUANG ; Zerong FENG ; Wei MAI ; Qianzi QIN
Clinical Medicine of China 2009;25(3):304-306
Objective To explore the value of Ligasure vessel sealing system(LVSS)during resection of retroperitoneal tumor.Methods Group 1 including 32 cases were performed resection of retroperitoneal tumor with LVSS and electrosurgical seapel from Jun.2004 to Oct.2008.group 2 including 26 cases were done with electrosurgieal seapel from Jan.2001 to Jun.2004.Operating blood loss,operating time,iatrogenic injury,postoperative blood loss and hospital stay were compared between the two groups.Results There was no significant difference in hospital stay[(11.7±0.7)d vs.(12.3±1.4)d)]and iatrogenic injury(9.38%vs.16.00%)between two groups statistically(P>0.05),but the intraoperative blood lose[(403.1±37.1)ml vs.(704.0±129.0)m1)s,postoperative blood loss[(131.5±18.4)ml vs.(214.8±29.2)ml)]and operating time[(166.5±8.9)min vs.(186.8±15.4)min]were less in group 1 than that in group 2(P<0.05).Conclusion Ligasure vessel sealing system has advantages of safe coagulation,shortening operation time in resection of retroperitoneal tumor.
8.Effect of gene transfer of antisense hypoxia inducible factor-1? on chemosensitivity of human pancreatic cancer cell line BxPC-3
Qing CHANG ; Renyi QIN ; Jun GAO ; Yanping FENG ; Tao HUANG
Chinese Journal of General Surgery 1997;0(06):-
Objective To observe the effect of antisense hypoxia inducible factor-1?(HIF-1?) on (chemosensitivity) of human pancreatic cancer cell line BxPC-3 under hypoxia. Methods BxPC-3 cells were divided into 3 groups:(1)BxPC-3 cells were non-transfected with antisense HIF-1? plasmid and exposed to 0.5% O_2 for 4hr(hypoxia control);(2)normoxic BxPC-3 cells were non-transfected with antisense(HIF-1?) plasmid(normoxia control);(3)BxPC-3 cells were transfected with antisense HIF-1? plasmid and exposed to 0.5% O_2 for 4hr(experimental group).Expression of HIF-1? and survivin was detected by RT-PCR and Western Blot.Growth inhibition rates and apoptosis rates of BxPC-3 cells under different(dosages) of chemotherapeutic agents(5-fluorouracil,doxorubicin and gemcitabine) were measured by MTT(colorimetric) assay and flow cytometry (FCM).Results Expression of HIF-1? was obviously down-regulated and at the same time susvivin expression was markedly down-regulated in experimental group(P
9.Low temperature controlled plasma technology by nasal endoscopy in treatment of nasal vestibular cyst
Jin LIU ; Yun FENG ; Jixin QIN ; Huanlai HUANG
China Journal of Endoscopy 2017;23(7):6-10
Objective To compare the efficacy of low temperature controlled plasma technology by nasal endoscopy and cystectomy via labiogingival groove approach in treatment of nasal vestibular cyst. Methods 38 patients with nasal vestibular cyst from Jan 2013 to Nov 2016 were selected and randomly divided into control group (n = 20) and observation group (n = 18). The control group received cystectomy via labiogingival groove approach and the observation group received low temperature controlled plasma technology by nasal endoscopy. The time of surgery, bleeding volume in surgery, hospitalization, postoperative complications and relapse were collected and compared between the two groups. Results We found that the observation group have less surgery time than that in control group [(65.45 ± 27.51) vs (34.72 ± 17.61) min, P = 0.000], less bleeding volume in surgery [(22.35 ± 18.41) vs (3.17 ± 1.69) ml, P = 0.000), less postoperative complications [(6.35 ± 0.75) vs (3.61 ± 1.19) d, P = 0.000] and less postoperative complications (16 vs 1, P = 0.000), but relapse of the two groups have no statistical difference (1 vs 0, P = 0.783). Conclusions Low temperature controlled plasma technology by nasal endoscopy in treatment of nasal vestibular cyst have many advantages than cystectomy via labiogingival groove approach such as shorter time of surgery, less bleeding volume in surgery, shorter hospitalization and less postoperative complications. Therefore it's worth of applying clinically.
10.Mechanism of SIRT1 acting on tumor formation
Anning FENG ; Xiangshan FAN ; Qin HUANG ; Lihua ZHANG
Journal of Medical Postgraduates 2003;0(07):-
SIRT1 is an NAD+-dependent histone deacetylase,involved in many physiological processes,such as cell aging,apoptosis and differentiation.Recent studies have shown that SIRT1 plays a significant role in tumor formation and progression.It deacetylates a wide range of histone and non-histone substrates and regulates gene expression and protein activity that are associated with neoplastic apoptosis and proliferation.