In this study, the ultrastructural changes of skeletal muscle of hindlimbs in rats were observed. Skeletal muscle of 5 h ischemia showed diminished glycogen granules, swelling of mitochondria, clearing of mitochondrial matrix, breakage of mitochondrial crista and dilatation of sarcoplasmic reticulum. Skeletal muscle of 5 h ischemia and 1/2 h or 12 h reperfusion showed high amplitude swelling of mitochondria with rupture of mitochondrial membrane, dense granules in mitochondrial matrix and complete loss of normal organization of myofibrils. The results demonstrate that reperfusion after prolonged ischemia exaggerates tissue injury.

Objective To observe the clinical effect of treating chronic renal failure with herbal retention-enema.Methods 23 cases with chronic renal failure were randomly recruited into a control group and a treatment group. The control group was treated with routine western medicine (low salt, low fat, and low protein diet, and correcting disturbance of water-electrolyte and acid-base balance), and the treatment group was treated with herbal retention-enema (Rhubarb, Concha ostreae, Dandelion, and Processing aconite). The clinical effect was observed in both groups. Results The clinical effect in the treatment group was significantly higher than that of the control group (χ2=3.456, P＜0.05). Conclusion The treatment of chronic renal failure with herb retention-enema is better than routine western medicine.

Objective To approach effect of glucosidorum tripterygll totorum on chronic glomerulonephritis.Methods A total of 182 cases of chronic glomerulonephritis were randomly recruited into a treatment group (103 cases) and a control group (79 cases). The control group was treated with valsartan capsule, 80mg per time, once daily, and prednisone for those cases of intractable proteinuria, 1 mg/kg, once daily. The treatment group was treated with glucosidorum tripterygll totorum the basis of the control group. Therapeutic effects in both groups were evaluated. Results The total effective rate was 83.5% in treatment group and 65.8% in control group, showing significant difference (t=2.0134, P=0.0785) Conclusion Glucosidorum tripterygll totorum has sound therapeutic effects on chronic glomerular nephritis.

[Objective] To ensure the safety of preoperative autologous blood donation in late pregnancy through a series of nursing measures.[Methods]From January 2010 to November 2011, 52 cases of pregnant women in late pregnancy took storage autotransfusion. After prenatal guardianship, psychological care, health education, blood were col ected before and after care, custody and from the blood transfusion care; record blood col ection process pregnant women with maternal-fetal reaction, the vital signs of pregnant women, birth outcomes, since the amount of al ogeneic blood transfusion, and achieve satisfactory re-sults.[Results] 52 cases of pregnant women with the blood col ection process were good, normal, with stable vital signs, abnormal fetal heart rate monitoring, one case of fetal moved too much, given the oxygen, improved with the left lateral decubitus position. In the process of self-blood transfusion, there's no ma-ternal discomfort, but with safe motherhood. [Conclusion] Effective care measures and storage of a pregnant woman autologous transfusion are relatively safe, economical and effective in the transfusion way, achieve good results, and help to improve the quality of obstetric care.

Dipeptidyl Peptidase-4 inhibitor (DPP-4 inhibitor),a new kind of oral anti-diabetic medicine,has been widely used for the treatment of type 2 diabetes (T2DM) during the last decades.Recent evidence showed that DPP-4 inhibitor may have renal protective effects beyond glycemic control.Some clinical studies revealed that the reduction of HbA1c by DPP-4 inhibitor was paralleled by an improvement of albuminuria and renal function.Experimental studies indicated that the renal protection mechanism of DPP-4 inhibitor may be involved in its multiple effects including anti-inflammatory,anti-oxidation and improvement of endothelial function.However,the evidence of decreasing albuminuria for DPP-4 inhibitor mainly comes from animal models and some clinical trials with small sample size.More large randomized controlled trials (RCT) will be needed to certify the renal protection effect of DPP-4 inhibitor.In this review,the role of DPP-4 inhibitor on albuminuria will be considered and discussed from both experimental and clinical perspectives.

Objective:To construct and identify the recombinant eukaryotic plasmid PcDNA3.1-MxA encoding MxA with the eukaryotic plasmid PcDNA3.1.Methods:Recombinant plasmid PAdTrack-MxA constructed before and PcDNA3.1 were amplified in Escherchia coli JM109.Plasmids were double-digested with restrictive endonucleases NotⅠ and XbaⅠ after extracted,and then ligated to construct recombinant eukaryotic plasmid PcDNA3.1-MxA.The recombinant plasmid was selected for Ampicillin resistance and then confirmed by digestion with NotⅠ and XbaⅠ,PCR and sequencing.The sequence was homology-analysed compared with the sequence of MxA gene in PAdTrack-MxA and the sequence of MxA gene published in Genebank(NM_002462) with sofeware DNAssist 2.0.Results:The restrictive endonuclease and PCR analysis confirmed that the recombinant eukaryotic plasmid PcDNA3.1-MxA was constructed successfully.Sequencing analysis revealed that the cloned segment was 2012bp including MxA gene sequence,and the nucleotide sequence of MxA gene was same to that in PAdTrack-MxA exactly.There were five variations in nucleotide sequence compared with published sequence in Genebank,which caused only one amino acid residue replaced from isoleucine to valine.This replaced amino acid residue was located in the non functional area of MxA protein.Conclusions:The recombinant eukaryotic plasmid PcDNA3.1-MxA is constructed successfully,which lays foundation for the further study on anti-HBV effects of MxA.

The incidence of carotid stenosis disease increases with age,and it is one of the major causes of ischemic stroke.In recent years,with the rapid development of endovascular treatment technology,the therapeutic means,including carotid angioplasty and stenting,has been widely carried out both at home and abroad.This article reviews the advances in endovascular treatment of carotid stenosis.

OBJECTIVE:To identify Salvia miltiorrhiza,Rehmannia,Chuangxion rhizome,rhubarb and to determine the content of tanshinone Ⅱ A,as an efficacious composition,in Shendi Tangmaining capsules.METHODS:TLC and SPE-UV were used.The SPE-UV method was performed with silica gel column(1.1cm).The mobile phase consisted of dichloromethane-me_thanol(8∶2).The detected wavelength was 280nm.RESULTS:In the SPE-UV method,the standard curve for tanshinone Ⅱ A was linear in the range of 1.6～8?g/ml(r=0.9 999).The average recovery was 96.13% with RSD=0.80%.CONCLUSION:The method is simple,rapid and reliable for quality control of the capsules.

A totel of 120 patients with CT or MRI proved as acute cerebral infarction group of anterior circulation and 90 non-infarction patients as the control group were studied. The diameter,inter median thickness( IMT) ,peak systolic velocity ( PSV ) , end diastolic velocity ( EDV ) , early shrinkage acceleration time ( AT ) , resistance index ( RI) ,and pulsatility index( PI) of carotid artery,and whether there was arteriosclerotic plaque or stenosis,and the properties of plaque were examined with color Doppler ultrasound. In acute cerebral infarction group of anterior cir-culation,the IMT was thicker that in the control group(P<0. 01). The incidence of the film thickness,moderate-se-vere stenosis and instable carotid artery plaque in infaiction group of anterior circulation were obviousely higher than those in the control group(P<0. 01). Arterial complete or incomplete occlusion was observed in severe cases. The blood flow parameters showed that the PSV and EDV of common carotid and internal carotid in acute cerebral in-farction group of anterior circulation were lowerer than those in the control group. RI and PI of internal carotid in a-cute cerebral infarction group of anterior circulation were higher than those in the control group(P<0. 05),while AT was lower than those in the control group.

Objective To compare the two kinds of the single nostril transsphenoidal approach of minimally invasive surgery of pituitary adenoma and analyze the clinical data ,and then provide references for clinical diagnosis and treatment .Methods The prospective study methods were used in the study .A total of 54 cases of pituitary tumor patients were collected , they were randomly divided into microscope group ( 31 cases ) and neural endoscopic group (23 cases).The operation time,intraoperative blood loss,hospital stay,the resection rate,postoperative complications ( mainly observed transient postoperative insipidus and transient electrolyte disorder ) , the hormone levels , and the comparison the results of patients ,follow-up within 1 year were observed .Results The operation time of microscope group(121.3+19.4)min was longer than that of the neuroendoscopic group (78.5 ±7.8)ml,but the intraoperative blood loss in the microscope group was less than that of the neuroendoscopic group [(50.3 ±3.2) mL vs (78.5 ± 7.8)mL,t=0.993,1.032,all P>0.05].The postoperative hospital stay [(8.7 +1.1) d vs (9.1 +2.3) d,t=2.897,P>0.05],the resection rate(93.5% vs 91.3%,χ2 =3.191,P>0.05) had no significant differences be-tween the two groups .The postoperative complications ( including transient insipidus and transient electrolyte disorder ) also had no obvious differences .The analysis of the endocrine hormone levels of different tumors found that there were no significant differences between the microscope group and neuroendoscopic group .The levels of these pre operative and postoperative hormones were as follows:PRL[(387.3 ±100.8)μg/L vs (145.3 ±27.4)μg/L and (390.2 ± 133.7)μg/L vs (148.4 ±57.2)μg/L],GH[(63.4 ±5.7)μg/L vs (10.6 ±2.4)μg/L and (65.6 ±6.2)μg/L vs (12.4 ±1.6)μg/L],ACTH[(202.9 ±73.7)ng/L vs (38.5 ±4.6)ng/L and (206.8 ±78.6)ng/L vs (35.6 ± 2.5)ng/L],they all had significant differences (t =1.456,1.301,0.973,1.034,0.774,0,732,all P <0.05). Conclusion The curative effects of the two surgical treatment of pituitary adenoma have no obvious differences ,they have advantages of minimally invasive , safe and effective, but the microscopic surgery has less bleeding , while neuroendoscopic surgery can shorten the operation time .