For children suffering from primary nephrotic syndrome(PNS), podocyte is a crucial part of glomerular filtration barrier, whose injury usually causes proteinuria.The structural and functional integrity of podocyte cytoskeleton is the prerequisite of its normal physiological function.Recent studies demonstrated that under a certain pathological condition, B7-1, expressed in podocyte, can inhibit the activation of β1 integrin by competitively binding to the target site on the β1 integration, which may change the morphology and function of podocyte, consequently induced proteinuria.Other studies also have showed that abatacept, which selectively inhibit the cross-talk between B7-1 and β1-integrin,can reduce proteinuria in patients with B7-1-positive glomerular disease.These studies suggested that B7-1 may be a potential diagnostic biomarker and therapeutic target in proteinuria in PNS.This review summarizes the role of B7-1 expressed in podocyte in the pathogenesis of proteinuria and the possibility of clinical application in the future.

Objective:To prepare a quantified animal model of retinopathy of prematurity by economic and easy method. Methods:A total of 44 postnatal day 7 wistar rats were randomly assigned into 2 groups according to different oxygen inhalation concentration(n=22).Experimental group was exposed to 75% oxygen for 5 days,and them deprived of oxygen supply suddenly and exposured in air for 5 days.The Control group was exposed to room air in same experiment condition.The retinal vascular development and proliferation were evaluated by stretched preparation (ADPase stained retina)and eyeball cross-section,The expression of vascular endothelial growth factor(VEGF) was detected by immunohisto-chemical stain.Results: The central vessels became tortuous and constricted and the central avascular area increased in high-concentration oxygen environ-ment.But hypoxia environment made great vessels of retina broaden and abnormal curvature become obvious,neova- scularization was seen in the Experimental group.The average numbers of neovascular nuclei which breakthrough limiting membrane of amphiblestrodes in the Experimental group and the Control group were 32.800 0?0.767 8 and 2.750 0?0.716 4 respectively.Stain of VEGF was weaker in the control group than in the treated group.Conclusion:The rat modle of retinopathy of prematurity has powerful repeatability,high constancy and can be used in quantitative study.It is a suitable mode to study the pathogenesis and therapy of retinopathy of prema-turity.

Objective:To investigate the effects of hypoxia inducible factor-1?(HIF-1?)antisense oligonucleotides(ASODN)on the expression of vascular endothelial growth factor(VEGF)and retinal neovascularization in premature rat with retinopathy.Methods:A total of 80 postnatal day 7 wistar rats were randomly assigned into two group.The experimental group(n=40)were established as ROP mode group.The control group(n=40)was exposed to room air in the same experimental condition.HIF-1?ASODN was injected into rats'left cavum vitreum at 12、14、16 d in these two groups.Some of the experimental group were injected with saline at 14、16 d.So there were five groups of specimens including:normal eye(A),normal eye with HIF-1?ASODN(A1),ROP eye(B),ROP eye with HIF-1? ASODN(B1),ROP eye+saline(B2).The retinal vascular development and proliferation in five groups were evaluated by the retina stretch preparation of 15 newborn rats at 12、14、17 d respectively.Eyeball cross-section was staimed with hematoxylin and eosin(HE).The nuclei of proliferative retinal vessels of five groups were counted.The expression of VEGF and HIF-1?in retina by immunohisto-chemical stain were observed among all groups.Results:In ROP group(B)the central vessels became tortuous and constricted and the central avascular area increased at 12 d.At 14 d,neovascularization was seen,peaking at 17 d.ROP eye with HIF-1?ASODN(B1)presented the same as group A,but Neovascularization decreased obviously as compcured with group B.Retinal vessel shape of ROP eye+saline(B2)is same as group B.VEGF was mainly expressed in capillary endothelium endochylema and nucleus of ganglionic layer of retina and inner nuclear layer.The expressions of VEGF were weaker in group A, A1 than in group B,B2,with significant differences(P

OBJECTIVE To study the occurrence of patients′ respiratory system infection in ICU and its incidence influenced by nursing interference. METHODS The incidence of respiratory system infection of patients who were in ICU longer than 48 hours. were analyzed the normal nursing group with 461 cases from Jan to Dec 2006 were compared with the normal nursing with interference group with 490 cases from Jan to Dec 2007. So the results of two groups were compared by statistical analysis. RESULTS Twelve from 461 cases (the incidence of 2.6%) in normal nursing group had respiration system infection,vs 6 from 490 cases incidence of 1.2% in normal nursing with interference group. With nursing interference the incidence of patients′ respiration infection decreased obviously. There was significant difference between two groups (P

Objective In order to make clear the clinical characteristics of gallbladder carcinoma.Method 37 cases of gallbladder carcinoma treated in Xinhua hospital have been analyed in recent 5 years.Result The higher incidence was in the elderly and the ratio between femal and male is 2 1 to 1.Clinical symptoms include right upper abdominal pain,nausea,fever,jaundice and abdominal mass.With gallstones is about 81 1%.Conclusion BUS and CT are common diagnostic methods.BUS is more reliable in early case,but CT in late case.The type of operation and the result of treatment are dependent on the pathologic stage and grade.

Objective:To investigate the curative effect of Chinese medicine foot bath combined with foot massage on diabetic peripheral neuropathy (DPN).Methods:Seventy-six patients with DPN who received treatment in Lishui Hospital of Traditional Chinese Medicine from July 2018 to December 2019 were included in this study. They were randomly assigned to undergo either Chinese medicine foot bath combined with foot massage (combined treatment group, n = 38) or Chinese medicine foot bath alone (single treatment group, n = 38) for 4 successive weeks in addition to diet and exercise management, medication for lowing blood glucose level, microcirculation improvement, and neurotrophic treatment. Before and after treatment, Toronto Clinical Neuropathy Score, Michigan Diabetic Neuropathy Score, and combined foot score were compared between the combined treatment and single treatment groups. The curative effect was also compared between the two groups. Results:After 4 weeks of treatment, Toronto Clinical Neuropathy Score [(5.82 ± 0.57) points, (7.62 ± 0.89) points], Michigan Diabetic Neuropathy Score [(14.92 ± 2.16) points, (18.01 ± 2.87) points], combined foot score [(3.17 ± 0.49) points, (4.41 ± 0.52) points] in the combined treatment and single treatment groups were significantly lower than those before treatment [(9.84 ± 1.27) points, (9.65 ± 1.21) points, (23.09 ± 4.03) points, (22.81 ± 3.92) points, (5.62 ± 0.85) points, (5.76 ± 0.73) points, t = 3.137, 2.361, 2.952, 2.392, 3.341, 2.427, all P < 0.05]. The declines in the combined treatment group were significantly greater than those in the single treatment group (all P < 0.05). Total effective rate in the combined treatment group was significantly higher than that in the single treatment group (94.74% vs. 78.95%, χ2 = 4.150, P < 0.05). Conclusion:Chinese medicine foot bath combined with foot massage is effective for DPN, which can significantly improve the clinical symptoms and promote the recovery of neurological function.

Objective To study the efficacy of endoscopic retrograde cholangio-pancreatography (ERCP) and endoscopic nasobiliary drainage (ENBD) in the diagnosis and treatment of bile leakage after laparoscopic cholecystectomy (LC).Methods The clinical data of 9 patients with bile leakage after LC were analyzed retrospectively.Results The procedure of ERCP was successful in all 9 patients.Bile leakage was treated successfully by ENBD in 8 patients with no further complication,and 1 patient was treated surgically because of biliary ductal injury.Conclusions ERCP is recommended as a safe and efficacious option to detect and treat bile leakage after LC.ENBD combined with percutaneous drainage of abdominal collections can cure bile leakage after LC.

Objective: To explore the effects of Qichu Fujin Recipe (QCFJR), a compound traditional Chinese medicine, in repairing sciatic nerve injury in rats. Methods: A total of 60 male Sprague-Dawley (SD) rats were randomly divided into four groups: normal control group, untreated group, mecobalamin group and QCFJR group. Except the normal control group, sciatic nerve injury was induced by crushing of left sciatic nerve. Rats in the mecobalamin group were intragastrically administered with mecobalamin solutions 150 mug/(kg.d), and rats in the QCFJR group were intragastrically administered with QCFJR 35.2 g/(kg.d), while rats in the untreated group were intragastrically administered with normal saline (0.5 mL/d), once a day for 4 weeks respectively. Sciatic function index (SFI) was determined by walking tract analysis 1 week and 2 and 4 weeks after crushing. Five rats in each group were sacrificed for histological observation at the different time points. The remnant rate of gastrocnemius wet weight and the diameter of gastrocnemius cells were calculated. Expression of S-100 protein in the distal stump of injured nerve was observed by using immunohistochemical method. Distal injured sciatic nerves were determined with toluidine blue staining and observed under a light microscope 1 week and 2 and 4 weeks after operation. Diameter of axon and depth of myelin sheath were calculated by an image analysis system. Ultrastructure of nerve fibers was determined with uranyl acetate and lead citrate staining and observed under an electron microscope. Results: Compared with the normal control group, SFI, remnant rate of gastrocnemius wet weight, diameter of gastrocnemius cells, expression of S-100, diameter of axon and depth of myelin sheath in the mecobalamin group and the QCFJR group were significantly decreased at different time points (P<0.05), superior to those of the untreated group (P<0.05, P<0.01). Except the remnant rate of gastrocnemius wet weight and the diameter of gastrocnemius cells, there were no significant differences in other indexes between the mecobalamin group and the QCFJR group 1 week and 2 and 4 weeks after crushing (P>0.05). Conclusion: QCFJR can stimulate the regeneration and repair of nerve fiber and delay the skeletal muscle atrophy after sciatic nerve injury in rats.

Objective To establish the model of alcohol-induced hepatocyte steatosis in L-02 liver cells in vitro,investigate the relationship between cell damage,UCP2 gene expression and ethanol concentrations in L-02 cells of steatosis,and explore the protective effect of Hovenia Dulcis Thunb extracted liquid.Method Hovenia Dulcis Thunb extracted liquid at the most compatible dosage was applied to the L-02 cells of steatosis.Then UCP2 mRNA was detected by the semi-quantitative RT-PCR method.Results In steatosis model groups,UCP2 mRNA expression was downregulated ( P ＜0.05).In the steatosis groups treated with Hovenia Dulcis Thunb extracted liquid,UCP2 mRNA level was significantly increased compared to steatosis group ( its expression level increased from 0.4859 to 0.7442 after treatment of 0.6％ ethanol,P＜ 0.05).Conclusions Ethanol can down-regulate the UCP2 mRNA expression of L-02 cells in a certain period.The low expression of UCP2 mRNA is probably relevant to the occurrence of alcoholic fatty liver disease.Hovenia Dulcis Thunb can prevent from the cell toxicity of ethanol and decrease the degree of steatosis.Hovenia Dulcis Thunb extracted liquid can increase the expression level of UCP2 mRNA in the L-02 cells.

BACKGROUND:Fetal liver stem cel s have the potential to differentiate into hepatocytes and bile duct cel s, and participate in the repair and reconstruction of the liver, which is an important source of hepatocytes. But there are a little amount of fetal liver stem cel s in human body, and how to obtain a certain number of high-purity fetal liver stem cel s is currently a hot research. OBJECTIVE:To construct a siRNA carrier that can effectively inhibit the expression of connexin 43 (Cx43) in rat fetal liver stem cel s, and to investigate the effect of Cx43 inhibition on the proliferation and cel cycle of fetal liver stem cel s cultured in vitro. METHODS:Fetal liver stem cel s were cultured by the suspension culture in vitro, siRNA sequences targeting Cx43 (Cx43-siRNA) and negative control sequence (NC-siRNA) were designed and synthesized. Then, rat fetal liver stem cel s were transferred electrophoretical y and divided into three groups:blank group, NC-siRNA group, Cx43-siRNA group. Real-time PCR and western blot were used to assess the knockdown efficiency. Cel ular proliferation was determined by cel growth curve and cel counting kit-8 assay. The cel cycle was analyzed by flow cytometry. RESULTS AND CONCLUSION:After transfection, the Cx43 gene and protein expression levels were declined dramatical y in the Cx43-siRNA, NC-siRNA and blank groups, and the cel s grew faster. The number of cel s at G0/G1 phase decrease, but the number of cel s in S phase increased. There were significant differences between the groups (P<0.05). Electrophoretic transfer of Cx43-siRNA can promote the proliferation of cultured fetal liver stem cel s and optimize the cel culture.