Objective To study the respiratory tract virus infection in dengue fever patients in Guangzhou. Methods Totally 280 patients with dengue fever in 2014 and 32 patients from May to December 2015 were select-ed as group A and group B respectively. The distribution of clinical symptoms and signs ,age and onset time of the 2 groups were compared. Simultaneously ,other respiratory viruses in all patients were detected. Results In group B,the incidence of fever,bone pain,myalgia/body weight fatigue,anorexia,diarrhea,rash diagnosed and hemorrhage was higher than that in group A. The incidence difference of headache,bone pain,myalgia/body weight fatigue,anorexia and rash in 2 groups indicated no statistical significance(P > 0.05)but the incidence of anorexia was significantly different(P<0.05). In group A,there were a larger proportion of patients aged from 21to 30(28.57%)and from 31 to 40(25.36%)while in group B,there were a larger proportion of patients aged from 41 to 50 (19.69%) and from 51 to 65 (25.00%). There was no significant difference in the distribution of other age groups(P > 0.05)except that of 51 ~65 years in 2 groups(P < 0.05). In 2014,the peak inci-dence of dengue fever was in October (64.29%),followed by November (28.21%) and in 2015 ,October was the peak incidence of dengue fever (84.38%),followed by November (12.49%). The incidence in 2 groups in October was significantly different (P < 0.05). In 280 cases of group A ,positive rate of 9 respiratory virus was 30.36%(85/280) and there were 38 cases with MP (44.71%) and 24 with LP1 (27.24%);in 32 cases of group B ,positive rate of 9 respiratory virus was 32.50%(11/32) and there were 4 with MP (36.36%) and 3 LP1(27.27%). The detection rate of other viruses indicated no significant difference(P > 0.05). Conclusions The incidence of dengue fever is different in Guangzhou area at different time. The patients present different clinical symptoms and signs. Analysis of different pathogenic characteristics provides important basis for clinical treatment of dengue fever.

Antineoplastic Agents ; therapeutic use ; Colorectal Neoplasms ; drug therapy ; genetics ; metabolism ; Genes, ras ; genetics ; Humans ; Lung Neoplasms ; drug therapy ; genetics ; metabolism ; Molecular Targeted Therapy ; methods ; Mutation ; Neoplasms ; drug therapy ; genetics ; metabolism ; Pancreatic Neoplasms ; drug therapy ; genetics ; metabolism ; Proto-Oncogene Proteins ; genetics ; metabolism ; Proto-Oncogene Proteins p21(ras) ; Receptor, Epidermal Growth Factor ; drug effects ; metabolism ; Signal Transduction ; ras Proteins ; genetics ; metabolism

To construct recombinant reporter plasmids containing different Alpha gene segments and Alpha1-TFEB fusion gene and to evaluate the promoter activity of the Alpha gene. Methods:Promoter regions of the Alpha gene were predicted using a software Primer 0.5. Five Alpha gene segments with different lengths and a normal TFEB gene promoter (pTFEB) were amplified via polymerase chain reaction, and recombinant reporter plasmids containing different Alpha gene segments and a normal TFEB gene pro-moter were constructed. Liposome transfection was used to transfect these vectors into the human embryo kidney 293T cells. The pro-moter activity of the Alpha gene was evaluated via luciferase assay. Meanwhile, the recombinant Alpha1-TFEB plasmid was construct-ed and transfected into the 293T cells. The TFEB expression of the recombinant Alpha1-TFEB plasmid was then detected via Western blot. Results: Recombinant reporter plasmids containing different Alpha gene segments and pTFEB were constructed successfully. Compared with the luciferase activity of pGL3-Basic, that of the groups with Alpha1, Alpha2, Alpha3, Alpha4 and Alpha5 significantly increased (P<0.01). The luciferase activity also increased significantly in the groups with Alpha1, Alpha2 and Alpha5 compared with that of the pTFEB group (P<0.01). The TFEB expression of the pGL3-Enhancer-Alpha1-TFEB was significantly higher compared with that of the pGL3-Enhancer group. Conclusion:In t(6;11) translocation RCC, the Alpha gene has a strong promoter activity and it en-hances TFEB expression. The strongest promoter activity region is in Alpha5 with a sequence from 643 bp to 693 bp.

Objective To let students grasp comprehensive applied knowledge and the new development.Methods A special website was set up based on the traditional teaching.Results The special website could provide students with a quick,convenient and modern technology resource library.Conclusion The website can effectively help students consolidate and deepen the study of basic theory and technology of medical imaging equipment,and broaden their knowledge.

Objective To design a set of long bone and bone-mode auto-matching system which is based on computer image processing technology.Methods Through the analysis on clinical pictures and image data,we got the exact data.Based on these data,the system searched out the bone-mode from the bone-mode database which matched with the skeletal.Results It can find a suitable bone-mode which can match with the bone in a very short time.Conclusion The system improves the efficiency of matching,as well as the success rate.It reduces the surgery time and the suffering of patents.

Objective To research the effect of the baicalin to the expression of interferon(IFN)-α and IFN-β in the BALB/c mouse infected by respiratory syncytial virus(RSV),and discussed the antiviral of mechanism.Methods To establish the BALB/c mouse model of RSV,the effects of IFN-α and IFN-β in the BALB/c mouse infected by RSV were detected by ELISA.Results The IFN-α and IFN-β in the BALB/c mouse infected by RSV could be evaluated significantly by Baicalin.Conclusion The Baicalin regulates the level of IFN in the BALB/c mouse infected by RSV.

Objective To establish a sensitive, reliable and simple method for determination of selenium(Se) in salt. Methods The salt samples were dissolved in water and added hydrochloric acid, thiourea, placed for 30 minutes under the room temperature, then used HG-AFS to determine Se. Results The linear range was 0.5-20 ?g/L, the detection limit was 0.06 ?g/L, RSD

Objective The present paper makes attempt to study the enrichment features of mercury in freshwater fishes and to evaluate the mercury contamination in environment with the fishes.Methods The fish samples of silver carp,common carp,johnny carp,shorthead catfish and catfish were collected from Songhua River and Heilongjiang River.The content of mercury in freshwater fishes,water and sediment was determined,the biological characteristic of mercury contamination in aquatic animal and the applicability of mercury contamination in aquatic amimal and its estimation were analyzed in Sep.,2007.Results The levels of mercury in river water and sediment were 0.053 ?g/L and 0.27 mg/kg for samples collected from Jiamusi section of Songhua River,and 0.034 ?g/L and 0.16 mg/kg for samples collected from Fuyuan section of Heilongjiang River.The concentrations of total mercury in the muscles of Songhua River’s silver carp,common carp,johnny carp,shorthead catfish and catfish was(0.070 ? 0.036),(0.120?0.071),(0.113?0.067),(0.152?0.107) and(0.196?0.095) mg/kg respectively.The concentrations of total mercury in the muscles of Heilongjiang River ’s silver carp,common carp,johnny carp,shorthead catfish and catfish was(0.042 ? 0.034),(0.103?0.077),(0.095?0.072),(0.157?0.118),(0.174?0.101) mg/kg respectively.The mercury content in fish in the same water environment was significantly positive correlated with its weight(P

AIM: To investigate the efficacy of 23G minimally vitrectomy without irrigation in cataract phacoemulsification and trabeculectomy of malignant glaucoma, and to analyze such compound operative procedures for phakic malignant glaucoma.
●METHODS:A total of 21 phakic malignant glaucoma patients (21 eyes) underwent anterior vitrectomy without irrigation by using 23G vitrectomy. During surgical course phacoemulsification with anterior and posterior continuous circular capsulorhexis, trabeculectomy combined with iridectomy would be completed. lntraocular pressure, anterior chamber depth changes and postoperative complications were observed after the operation.
●RESULTS:ln the three-month follow-up, intraocular pressures were reduced from ( 57. 18 ± 6. 18 ) mmHg to (16. 15 ± 2. 43 ) mmHg, there was statistical difference compared with pre - operation ( P < 0. 001 ). The preoperative anterior chamber depth (ACD) was (0. 88± 0. 25) mm, the postoperative ACD was (2. 44±0. 37) mm 3mo later, there were significant difference (P<0. 001). The best corrected visual acuity improved significantly, no serious postoperative complication appeared.
● CONCLUSION: The compound surgical method of anterior vitrectomy combined with phacoemulsifier and trabeculectomy can effectively treat phakic malignant glaucoma. Early diagnosis and early compound surgery may effectively reduce the intraocular pressure of malignant glaucoma.

Transcuataneous electrical nerve stimulation (TENS) analgesia as a non-drug method has received people's more and more attention recently. Considering problems of existing products, such as unstable performance and unsatisfied effectiveness, we developed a new analgesia therapy system for delivery based on bio-feedback TENS in our laboratory. We proposed a new idea for stimulation signal design, that is, we modulated a middle frequency signal by a traditional low frequency TENS wave in the new system. We designed different prescription waves for pain relief during a uterine contraction or massage between contractions. In the end, a bio-feedback TENS method was proposed, in which the waveforms of stimulation signals were selected and their parameters were modified automatically based on feedback from uterine pressure, etc. It was proved through quality tests and clinical trials that the system had good performance and satisfied analgesia effectiveness.
Analgesia ; methods ; Biofeedback, Psychology ; methods ; Female ; Humans ; Pain Management ; Transcutaneous Electric Nerve Stimulation ; methods ; Uterine Contraction