1.Effects of hyperbaric oxygen on choline acetyltransferase in neurons after traumatic brain injury
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(05):-
Objective To observe the effect of hyperbaric oxygen on choline acetyltransferase ChAT levels in the neurons of rats with traumatic brain injury. Methods Traumatic brain injuries were modeled in rats using Feeney's method. Thirty male rats were randomly divided into 3 groups: a traumatic brain injury group (group A) , a traumatic brain injury plus hyperbaric oxygen ( HBO) group ( group B) , and a sham operation group (group C). Samples of each rat's brain were tested for ChAT using immunohistochemical staining and light microscopy. Results ChAT-positive fibers in the hippocampus almost vanished after traumatic brain injury, but positive fibers were markedly increased after HBO treatment. Conclusion HBO treatment can activate neurons in the hippocampus and inhibit regression of cholinergic neurons, which may be one of the mechanisms by which HBO therapy improves learning ability in injured rats.
2.Self-blood therap for 62 cases of senile skin pruritus.
Hui XIAO ; Jian QIN ; You-Xing ZHANG
Chinese Acupuncture & Moxibustion 2013;33(8):757-758
Acupuncture Points
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Aged
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Aged, 80 and over
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Bloodletting
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Female
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Humans
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Middle Aged
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Pruritus
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therapy
3.Clinical and coronary angiographic analysis of elderly patients with type 2 diabetes mellitus combined with coronary heart disease
Suxuan LIU ; Yongwen QIN ; Xiaohua YOU
Academic Journal of Second Military Medical University 1981;0(03):-
Objective:To analyze the association of type 2 diabetes mellitus(DM) with the clinical and coronary angiographic features of coronary heart disease(CHD) in patients aged over 70 years old.Methods: A total of 310 elderly patients with coronary angiograph-confirmed coronary diseases,who were treated in Changhai Hospital during Apr.2006 to Jul.2008,were retrospectively analyzed.The patients were further divided into 2 subgroups according to the presence of DM: DM-CHD group(n=155) and non-DM-CHD group(n=155).The age,gender,blood pressure,blood lipid,ejection fraction(EF),the angiographic outcomes,etc.were analyzed and compared between the two groups.Results: The incidence of hypertension was significantly higher in the DM-CHD group than in the non-DM-CHD group(P
6.Delayed gastrointestinal transit time and changes of ileum myenteric plexus in diabetic rats
Ya-Ning LEI ; You-Mei DING ; Xu QIN ;
Chinese Journal of Digestion 2001;0(10):-
Objective To investigate the morphologic abnormalities of myenteric plexus in diabetic rats and to explore the mechanism of its effect on gastrointestinal motility.Methods Thirty rats were randomly divided into diabetic group and control group.Gastrointestinal transit time was measured and histologic changes of cholinergic and nitriergic nerves in ileum myenteric plexus were observed with enzy- matic histochemistry.Results Four months after the establishment of the diabetic rats model,gastroin- testinal transit time was found delayed comparing with control group,the density of cholinergic neurons in the ileum myenteric plexus was decreased (P<0.01) and the densities of nitriergic ganglions and neurons were significantly increased comparing with control group (P<0.05 and P<0.01).Conclu- sion Decrease of cholinergic nerves and increase of nitriergic nerves in the myenteric plexus of intestine is one of the mechanisms of delay gastrointestinal transit time in diabetic rats.
7.Evaluation of the relationship of SMT injection and cytokines expression for temporomandibular joint osteoarthrosis
Xianan QIN ; Weichi MAI ; Manqiu YOU ; Aifang ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2008;15(z1):15-16
Objeetlve To evaluate the relationship between temporomandibular joint(TMJ)osteoarthrosis (OA)and cytokines.Methods 20 rabbits were enrolled in this study.They were divided into either experiment(n=10)or control group(n=10).The modeh of TMJ osteoarthrosis were formed by injection SMT into upper joint compartment of the TMJ.Cytokines in the aspirated synovial fluid,induding interleukin(IL-1),IL-6,tumor necrosis faetor(TNF-α),were tested before and after SMT iniection.Results The level of IL-1β,IL-6 and TNF-α in experiment group decreased significantly after treatment,there was significance(P<0.05);and the level of IL-1β,IL-6 and TNF-α in control group is lower after treatment,there was no significance(P>0.05).Conclusion The cytokines,including IL-1β,IL-6,TNF-α,decreased significantly by injection SMT into upper joint compartment of the TMJ with OA.
8.Inhibitory effect of cajanonic acid A on lipogenesis and lipolysis in 3 T3-L1 adipocytes
You QIN ; Ruiyi YANG ; Meiguo CHEN ; Xiaoling SHEN ; Yingjie HU
Chinese Pharmacological Bulletin 2016;(2):189-193
Aim To investigate the effects of cajanonic acid A (CAA) on lipid metabolism in murine 3T3-L1 adipocytes. Methods 3T3-L1 cells induced to differ-entiated into mature adipocytes were treated with CAA in different dosages for 48 h, then total lipids as well as triglyceride, free fatty acid and glycerol were meas-ured. The expression levels of genes related to lipid metabolism were quantitatively analyzed by real-time fluorescent quantitative polymearase chain reaction ( RTFQ-PCR) . Results Total lipids and triglyceride in 3T3-L1 adipocytes were markedly reduced by CAA. The release of free fatty acid and glycerol was lower than that of control. This coincided with decreased mRNA levels of the key enzymes involved in de novo lipogenesis ( acetyl CoA carboxylase and fatty acid syn-thase) , fatty acid uptake ( lipoprotein lipase) , and li-polysis ( hormone sensitive lipase and adipose triglycer-ide lipase ) . While the expression of fatty acid oxida-tive genes including acyl CoA oxidase and carnitine palmitoyl transferase1 was increased after CAA treat-ment. Conclusion CAA may inhibit lipogenesis and lipolysis,reduce circulating free fatty acid and improve the lipid metabolism in adipocytes by regulating gene expressions.
9.The approach on methods of replacing tracheostomy tube after incision of trachea in 48 hours
Yalin LI ; Weihua QIN ; Jiajun SUN ; Haiying YOU ; Lingling WANG
Chinese Journal of Postgraduates of Medicine 2009;32(22):29-31
Objective To explore the more secure and available methods in replacing traeheostomy tube.Methods Sixty patients with incision of trachea were divided into group A(29 eases)and group B (31 cases)randomly.The new method was used in group A with putting a catheter into the old tracheostomy tube before taking it out,and placing the new tracheostomy tube under the guide of catheter.While the traditional method was used in group B.The heart rate,SpO2,manipulation time,perioperative haemorrhage, and the condition of entering the false passage were recorded.Results The heart rate,SpO2 were no significant difference before and after manipulation in group A,but the heart rate increased and SpO2 decreased in group B(P<0.01 or<0.05).The manipulation time was(50.5±4.2) s in group A,and (84.9±5.3) s in group B(P<0.05).The perioperative haemorrhage >3 ml and the condition of entering the false passage in group A(2 Cases,o cage)were less than those in group B(15 cases.5 cases)(P<0.01 or<0.05).Conclusion The new method in replacing tracheostomy tube which use an input catheter is more security and more availability.
10.Clinical comparative study of postoperative early enteral nutrition and parenteral nutrition in elder patients with esophageal and cardiac cancer
Jun YOU ; Weixia QIN ; Peiren WU ; Ming HONG ; Jiangfeng QIU
Parenteral & Enteral Nutrition 2010;17(2):78-80
Objective:To compare the clinical value of early enteral nutrition(EEN) with total parenteral nutrition(TPN) in postoperative elder patients with esophageal and cardiac cancer. Methods: 102 cases of postoperative elder patients with esophageal and cardiac cancer were randomly divided into EEN group(n=51)and TPN group(n=51).The weight loss,serum albumin, prealbumin,liver function were measured before operation and on the eighth day after operation. The anal exsufflation time, infectious complication, duration of hospital stay and treatment cost were observed. Results: The weight loss in EEN group were less than those of TPN group(P<0.05). The levels of ALT, AST, BIL and GGT in EEN group on the eighth day after operation was lower than those in TPN group(P<0.05). The anal exsufflation time and duration of hospital stay in EEN group were shorter than those of TPN group(P<0.05). The treatment cost of EEN group was significantly less than that of TPN group(P<0.05). The infectious complication rate of EEN group was lower than that of TPN group(P<0.05). Conclusion: EEN in postoperative elder patients with esophageal and cardiac cancer can decrease the postoperative infectious complication and the treatment cost, shorten the duration of hospital stay, improve nutritional status and recovery of gastrointestinal function with less side effects of liver function.