1.Diabetes mellitus and hemoglobin A3.
Chinese Medical Journal 1979;92(9):639-646
Adult
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Blood Glucose
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analysis
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Diabetes Mellitus
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blood
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Electrophoresis, Agar Gel
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Electrophoresis, Paper
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Female
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Hemoglobin A
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analysis
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Humans
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Male
2.The therapeutic effects of portal aterialization and complete shunt in portal hypertension dogs
Gang CAO ; Wenbin YANG ; Yiming LI ; Zhaoyin QIN
Journal of Xi'an Jiaotong University(Medical Sciences) 2004;0(05):-
Objective In order to investigate the therapeutic effects of portal arterialization for portal hypertension,portal arterialization and complete shunt(PACS) was applied in canine model of portal hypertension,which was made by thread embolization within the portal vein.Methods A splenectomy,splenic artery and upper portal vein anastomosis,and a complete portal-caval shunt were performed on portal hypertension dogs.The blood pressure and flow of the portal vein including that towards the liver and towards the vena cava were observed.Results The postoperative hepatic inflow,PVF,increased to 180% of the former while PVP increased to 196%;the caval-inflow PVF increased to 130% of the former while PVP decreased to 45.5%.Significant difference existed(P
3.Splenic Portal Arterialization in Treatment of Portal Hypertension
Gang CAO ; Wenbin YANG ; Yiming LI ; Zhaoyin QIN
Chinese Journal of Bases and Clinics in General Surgery 2003;0(06):-
Objective To study the effect on liver hemodynamics of portal arterialization and complete shunt(PACS),splenorenal shut(SRS) and peripheral cardia divided vessel(PCDV).Methods The preparation of canine model was made.Group PCDV accepted a splenectomy and peripheral cardia divided vessel,while the group SRS accepted a spleen-renal vein shunt.Group PACS accepted a splenectomy,splenic artery and upper portal vein anastomosis,and complete portal-caval shunt.The blood pressure and flow of the portal system were observed.The hepatic function was also measured before and 2 weeks after the three kinds of operation.Results In the PCDV group,the postoperative PVF decreased in 17% while PVP decreased in 5%.In the SRS group,the postoperative PVF decreased in 51% while PVP decreased in 51%.In the PACS group,the postoperative hepatic inflow PVF increased to 180% of the former while PVP increased to 196%;the caval-inflow PVF increased to 130% of the former while PVP decreased to 46%.The results of PACS group had a magnificent statistic difference comparing with those two traditional operations(P
4.Low caveolin-1 predicts the poor outcome in patients with acute ischemic stroke
Kejin YIN ; Liqin LUAN ; Wenbin WANG ; Qin YIN
International Journal of Cerebrovascular Diseases 2016;24(8):699-703
Objective To investigate the correlation between the baseline serum caveolin-1 (Cav-1) levels and the clinical outcome in patients with acute ischemic stroke at day 90 after onset. Methods Consecutive patients with acute ischemic stroke treated in hospital were enrolled. The Cav-1 levels were measured by the enzyme-linked immunosorbent assay. Functional outcomes were assessed at day 90 after onset using the modified Rankin Scale (mRS), and mRS 0-2 was defined as good outcome. The demographic and clinical characteristics of the good outcome group and the poor outcome group were compared. Multivariate logistic regression analysis was used to identify the correlation between the low serum Cav-1 levels and the poor outcome at day 90 after onset. Results A total of 169 patients with acute ischemic stroke were included in the analysis, including 116 males (68. 5% ). Their age ranged from 50 to 83 years (mean, 63. 4 ± 9. 1). The mean serum Cav-1 level was 5. 483 ± 2. 617 ng/ml. Eighty-three-patients (49. 1% ) were in the good outcome group and 86 (50. 9% ) were in the poor outcome group. Univariate analysis showed that the proportions of patients in subcortical infarct (60. 5% vs. 43. 4% ; χ2 = 4. 944, P = 0. 026) and diffusion-weighted imaging-Alberta Stroke Program Early CT Score, (DWI-ASPECT) ≤7 (58. 1% vs. 40. 9% ; χ2 = 4. 984, P = 0. 026), as well as age (65. 5 ± 8. 9 years vs. 61. 2 ± 10. 7 years; t = 2. 793, P =0. 006), baseline systolic blood pressure level (140. 6 ± 17. 2 mmHg vs. 134. 9 ± 13. 4 mmHg; t = 2. 368, P = 0. 019; 1 mmHg = 0. 133 kPa), and National Institutes of Health Stroke Scale (NIHSS) score (4. 8 ± 3. 4 vs. 3. 9 ± 2. 2; t = 2. 211, P = 0. 036) were significantly higher than those of the good outcome group, and the serum Cav-1 level was significantly lower than that of the good outcome group (4. 9 ± 2. 3 ng/ml vs. 6. 1 ± 2. 1 ng/ml; t = 2. 977, P = 0. 003). After adjusting for the related confounding factors, multivariate logistic regression analysis showed that the low Cav-1 level was an independent predictor of poor outcome at day 90 in patients with acute ischemic stroke (odd ratio, 1. 157, 95% confidence interval 1. 016-1. 319;P = 0. 028). Conclusion The low serum Cav-1 level is an independent predictor of poor outcome in patients with acute ischemic stroke.
5.Study on relationship between polymorphism of TNF-?and IgA nephropathy of Han nationality Chinese in inner Mongolia Autonomous Region
Cheng WANG ; Caili WANG ; Lijun GAO ; Tianlin JU ; Wenbin QIN
Chinese Journal of Clinical Laboratory Science 2006;0(03):-
Objective To study the relationship of tumor necrosis factor beta (TNF-?) polymorphism with human IgA nephropathy of Chinese Han nationality in Inner Mongolia Autonomous Region. Methods The A→G single base mutation polymorphism in TNF-?1096 locus were analyzed among 80 normal controls and 79 IgA nephropathy patients by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The genotype frequencies of TNF?* 1/1 and TNF?* 2/2 in IgA nephropathy patients were significantly higher than that in normal controls (x1/12=5.58,P
6.Effects of grain-sized moxibustion from 7 am to 9 am on circadian rhythm of inflammatory factor IL-6 in rats with rheumatoid arthritis.
Wenbin MA ; Xuguang LIU ; Yong QIN ; Haiyan ZHOU ; Xin YANG
Chinese Acupuncture & Moxibustion 2016;36(4):396-401
OBJECTIVETo explore the rhythm regulatory mechanism of interleukin-6 (IL-6) in the process of moxibustion for rheumatoid arthritis (RA).
METHODSA total of 144 Sprague-Dawley (SD) rats were randomly divided into a blank group, a model group, a moxibustion group, a sham operation group, an operation group, an operation+moxibustion group, 24 rats in each one. Each group was divided into 4 time points (0:00 am, 6:00' am, 12:00 am, 6:00 pm), 6 rats in each time point. The Light-Dark 12 : 12 was given in all rats for light-dark cycle. Except the blank group, rats in the remaining groups were treated with intracutaneous injection of freund's complete adjuvant at right-side foot to establish the model of RA. After the model establishment, bilateral adrenal, glands were removed in the operation group and operation + moxibustion group, while those in the sham operation group were not removed with identical operation procedure. Rats in the moxibustion group and operation + moxibustion group were treated with grain-sized moxibustion from 7:00 am to 9:00 am at "Shenshu" (BL 23) and "Zusanli" (ST 36) once everyday, 6 times were taken as one session and 3 sessions were required tatclly, while rats in the remaining groups received identical fixation without moxibustion. The general health state and foot volume of rats were measured before model establishment, after establishment and after treatment. After treatment, rats were sacrificed at each time point to collect the blood sample and measure the content of IL-6 by using enzymne-immunoassay method.
RESULTSCompared with the blank group, the foot swelling in the model group was obviously increased (P<0. 05); the IL-6 maintained circadian rhythm (P<0. 05), but the peak phase had a backward trend, famplitude had an increased trend and the median was significantly lifted (P<0. 05). Compared with model group, !the foot swelling in the moxibustion group was obviously decreased (P<0. 05); the IL-6 maintained circadian. rhythm (P<0. 05), and the peak phase had a forward trend, amplitude had a decreased trend and the median was significantly reduced (P<0. 05). Compared with the moxibustion group, the foot swelling in the operation--moxibustion group was obviously increased (P < 0.05); the IL-6 maintained circadian rhythm (P < 0.5), but the peak phase moved forwrd, and the median was significantly elevated (P < 0.05).
CONCLUSIONThe IL-6 in plasma maintains significant pathological circadian rhythm in RA rats; with the complete hypothalamic-pituitary-adrenal axis, moxibustion is likely to regulate the circadian rhythm of IL-6 to play an important role of anti-inflammatory effect in RA rats.
Acupuncture Points ; Animals ; Arthritis, Rheumatoid ; metabolism ; physiopathology ; therapy ; Circadian Rhythm ; Disease Models, Animal ; Female ; Humans ; Hypothalamus ; metabolism ; Interleukin-6 ; metabolism ; Male ; Moxibustion ; Pituitary-Adrenal System ; metabolism ; Rats ; Rats, Sprague-Dawley ; Time Factors
7.A retrospective analysis of azathioprine in the treatment of 24 patients with refractory ulcerative colitis
Wenbin RAN ; Qin OUYANG ; Liefeng DONG ; Linyun XUE
Chinese Journal of Internal Medicine 2012;51(8):613-617
Objective To evaluate the efficacy and safety of azathioprine (AZA) in the treatment of refractory ulcerative colitis (UC).Methods Retrospective analysis of the clinical improvement,endoscopic improvement and mucosal healing rate,inflammation marker improvement after AZA administration and its safety in 24 refractory UC patients were performed,who were recruited between January 2007 and December 2011 in West China Hospital,Sichuan University,China.Results Twenty-four patients were enrolled,with a median age of 36 years old and a median course of 4 years.Among them,14 cases were moderate UC and 10 cases were severe UC.The patients were treated with AZA in a dose of (1.23 ±0.34) mg· kg-1 · d-1 from 7 weeks to 42 months.Efficacy was judged by Mayo disease activity index.At 3 months,6 months and 1 year after treatment,the effective rates were 73.9% ( 17/23),81.8%(18/22) and 14/16 respectively,and the remission rates were 17.4% (4/23),54.5% (12/22) and 12/16respectively.Both ESR and C reactive protein level after treatment for 6 months and 1 year were significantly lower than those before treatment [ (9.3 ±8.9) mrn/1h,(10.9 ±7.3) mm/1h vs (22.3 ± 10.7) mm/1h;2.5(1.0-22.3) mg/L,2.3(1.0-28.0) mg/L vs 18.4(3.6-137.0) mg/L; all P <0.05].Corticosteroid withdrawal rates at 3 months and 1 year after AZA treatment were 16/18 and 15/16,respectively.At 6 months and 1 year after AZA treatment,the endoscopic improvement rates were 85.7% ( 18/21 ) and 13/15 respectively; the cndoscopic remission rates were 61.9% ( 13/21 ) and 11/15 respectively; and the mucosal healing rates were 61.9% ( 13/21 ) and 11/15 respectively.Adverse effects were occurred in 8 patients.Leukopenia was the most common adverse effect,followed by liver function injury,alopecia and epigastric discomfort.Conclusions AZA is effective in the treatment of refractory UC patients with a low dose of ( 1.23 ± 0.34) mg· kg - 1 · d - 1,especially in the steroid withdrawing,maintaining remission and mucosal healing without severe adverse effects.
8.Evaluation criteria for admittance of medical techniques class Ⅰ and management in primary hospitals
Jun ZHANG ; Mei MEI ; Xueen YIN ; Wenbin ZHANG ; Dongmei SU ; Xianfeng ZHAN ; Qin WEI
Chinese Journal of Medical Science Research Management 2010;23(5):295-297
To enforce the application of medical techniques in the primary hospital so as to improve clinical quality and ensure clinical safety, we established the evaluation criteria for admittance of medical techniques class Ⅰ and the incentive system for new technique application. When both systems were applied, favorable results were obtained.
9.Study of synergistic effect of hyperlipoproteinemiaand Aβ in processing of Alzheimer′s disease
Jiangqi HOU ; Xin ZHANG ; Qin LONG ; Shifeng CHU ; Lei GUO ; Wenbin HE ; Junlong ZHANG ; Naihong CHEN
Chinese Pharmacological Bulletin 2017;33(4):498-506
Aim To research the synergistic effect of hyperlipoproteinemia and Aβ in the processing of Alzheimer′s disease.Methods Seventy SD rats were randomly divided into seven groups, and dealt with D-gal(hypodermic injection), hyperlipemia diet, microinjection into both side of CA1 section in hippocampus, independently.Morris water maze(MWM) test was used to evaluate the spatial memory impairments.Tau and tau(pThr181) pathology in the hippocampus were detected using Western blot and immunohistochemistry.Nissl′s staining was used to detect cell apoptosis.Results Aβ25-35-treated rats showed significant impairments of spatial memory in MWM test, especially in the group of D-gal+Aβ25-35+HLD(P<0.01).Furthermore, these rats treated with Aβ25-35, D-gal, and hyperlipemia diet, exhibited significantly increased phosphorylation of tau, particularly in the Thr181 site.Conclusion Hyperlipoproteinemia is the risk factor for older person, which could strengthen the toxic effect of Aβ, and promote phosphorylation of tau.
10.Correlation between polymorphisms of transforming growth factor-β1, tumor necrosis factor-related apoptosis-inducing ligand genes and nodular thyroid disease
Feng WEI ; Yonghong ZHANG ; Junfeng LI ; Xiaojing HUO ; Yueli YU ; Cuifeng WANG ; Wenbin QIN
Chinese Journal of Endemiology 2015;34(1):33-36
Objective To study the correlation between individual gene polymorphisms of transforming growth factor (TGF)-β1 + 869 T/C,tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) + 1525 G/A genes and nodular thyroid disease.Methods From September 2007 to December 2009,a total of 544 patients with nodular thyroid disease diagnosed in the Department of Endocrinology,The First Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology were selected,including 136 cases of nodular goiter patients (node group),132 cases of thyroid tumor (adenoma group),146 cases of Graves patients (GD group),and 130 cases of Hashimoto's thyroiditis (HT group).One hundred and thirty-five healthy subjects were enrolled as control group.Two milliliters of fasting venous blood of all subjects were collected.Polymorphisms of the TGF-β1 + 869 T/C and the TRAIL 1525 A/G genes were identified by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and the restriction fragment length polymorphism (PCR-RFLP) methods.Results TGF-β1 + 869 T/C:The CC genotypes and C allele frequencies of nodular goiter group [47.0%(64/136),63.2%(172/ 272)] were significantly higher than those of normal control group [18.0%(22/135),45.2% (122/270); x2 =30.76,17.79,all P < 0.05].The genotypes and allele frequencies of adenoma group[42.4% (56/132),59.1% (156/264)] were significantly higher than those of the normal control group (x2 =24.40,10.34,all P < 0.05).The risk of population carrying the C allele suffering from nodular goiter was 2.086 times of those carrying the T allele (OR =2.086; 95% CI:1.480-2.943).The risk of population carrying the C allele suffering from adenoma was 1.752 times of those carrying the T allele (OR =1.752,95% CI:1.244-2.469).TRAIL + 1525 G/A:the genotypes and allele frequencies of nodular goiter group [40.4% (55/136),62.9% (171/272)] were significantly higher than those of normal control group [12.0% (16/135),48.5% (131/270); x2 =9.176,11.307,all P < 0.05].The genotypes and allele frequencies of adenoma group[53.3% (70/132),73.1% (193/264)] were significantly higher than those of the normal control group (x2 =9.806,33.82,all P < 0.05).The risk of population carrying the G allele suffering from nodular goiter was 1.796 times of those carrying the A allele (OR =1.796,95% CI:1.275-2.531).The risk of population carrying the G allele suffering from adenoma was 2.884 times of those carrying the A allele (OR =2.884,95% CI:2.009-4.142).Conclusions TGF-β1 + 869 T/C and TRAIL + 1525 G/A gene polymorphisms may be related to the incidence of nodular thyroid diseases; G allele of TRAIL and C allele of TGF-β1 may be predisposing genes of patients with nodular goiter.