In this paper, the pharmacokinetics of ferulic acid loaded liposome-in-chitosan-microspheres was investigated. Eighteen Sprague-Dawley rats were divided into 3 groups randomly. Each group was administered orally of ferulic acid, ferulic acid loaded chitosan microspheres and ferulic acid loaded liposome-in-chitosan-microspheres, respectively. Then blood samples were obtained from fossa orbitalis at different time points. The concentration of ferulic acid in blood was analyzed by a HPLC method using coumarin as internal standard. The data were analyzed by DAS program. The t(max), MRT and t(1/2beta) of liposome-in-chitosan-microspheres were 2.500, 7.487 and 7.818 h, respectively, which were much longer than crude drug and chitosan microspheres. This results demonstrated that liposome-in-chitosan-microspheres had better sus-tained-releasing property. The AUC of liposome-in-chitosan-microspheres was 6.08 times higher than crude drug and 1.21 times higher than chitosan microspheres, which verified that liposome-in-chitosan-microspheres could enhance oral absorption.
Absorption ; Administration, Oral ; Animals ; Anticoagulants ; administration & dosage ; blood ; pharmacokinetics ; Biocompatible Materials ; Chitosan ; Coumaric Acids ; administration & dosage ; blood ; pharmacokinetics ; Delayed-Action Preparations ; Liposomes ; Male ; Microspheres ; Orbit ; blood supply ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Time Factors