Objective: To establish the determination of imperatorin and isoimperatorin in Tongketing Capsule (Radix Angelicae Dahuricae, Pheretima, etc.) by HPLC. Methods: The determination was carried out by HPLC. The mobile phase was acetonitrile water (60∶40) and UV detection wavelength was set at 245nm . The flow rate was 1.0mL?min -1 . The column temperature was 40℃ . Results: There were the good linear relationship between the absorption area value and the concentration in the range of 65?g～325?g for imperatorin and in the range of 50?g～250?g for isoimperatorin, respectively. The average recoveries were 98.3% ( RSD = 1.73% ) and 98.7% ( RSD = 1.65% ), respectively. Conclusion: The method is simple, accurate and can be used to determine imperatorin and isoimperatorin in Tongketing Capsule.

The structure, pharmaceutical functions and extraction and isolation methods of chlorgenic acid were reviewed to provide references for the research of chlorogenic acids and the production of the medicines chlorogenic acids.

OBJECTIVE:To investigate the status quo and problems of the construction of pharmacy staff team from Hubei Wuling mountainous area,and to provide reference for the formulation of construction plan of pharmacy staff team. METHODS:Using questionnaire survey and telephone interviews,the pharmacy staff of 22 hospitals at second level or above from Hubei Wul-ing mountainous area during 2011-2014 were investigated in terms of number,age,educational background,professional title,etc. RESULTS & CONCLUSIONS:The shortage of pharmacy staff is seious in 22 hospitals at second level or above from this areas (open 10 606 beds,should be staffed 956 pharmacy personnel,actual number of pharmacy personnel is 502);the age structure is unreasonable,and only 220 pharmacy staff aged under 35 year-old;highly educated pharmacy staff accounted for low proportion, and bachelor degree or above took up 29.5%;pharmacy staff with professional title took up low proportion,and pharmacy staff with high,middle and junior professional titles accounted for 1:5:4. The situation of pharmacy staff in the region can be improved through following measures,including striving for policy recommendations,continuously enriching the quantity of pharmacy staff, integrating scientifically and building reasonable talent tem gradually,introducing talents,optimizing education structure of pharma-cy staff,strengthening the training,improving operation level of pharmacy staff,etc.

Objective To explore correlation between gene polymorphism of TNF-alpha and thyroid associated ophtalmopa thy (TAO) in Zhuang ethnic population.Methods DNA and serum samples were collected in patients with TAO in Han/Zhuang population.ELISA was applied to detect level of TNF-alpha in serum and PCR was applied to detect polymorphism of TNF-alpha gene 863C_A.Results The levels of TNF-alpha in patients with TAO increased significantly compared with control group (P ＜ 0.05),and more significantly in Zhuang population compared with Han population (P ＜ 0.05) ;gene frequency of CA was significantly higher in patients with TAO in Zhuang population than that in Han population (P ＜ 0.05),while gene frequency of AA was significantly lower (x2 =0.770,2.145,all P ＜ 0.05).Conclusion Serum level of TNF-alpha increases during occurrence of TAO,and the differences of expression level between different nationalities are due to gene polymorphism of TNF-alpha.

Enterovirus 71 (EV71) is one of the primary causative agents of hand, foot and mouth disease in children and closely associated with severe neurological complications and even deaths.EV71 outbreaks have occurred throughout the Asia-Pacific region since 1990s, posing global public health threat;however, no specific therapeutic strategy exists for severe EV71 infection.Several inactivated vaccine products have entered or finished the clinical trial stage, and some novel vaccine candidates, including live attenuated, subunit, and virus-like particle, show great potential for further develop-ment.This review summarizes the present situation and progress in the development of EV71 vaccines.

Carcinoid Tumor ; pathology ; Groin ; Humans ; Laryngeal Neoplasms ; pathology ; Lymphatic Metastasis ; Male ; Middle Aged

By reviewing its experimental studies as well as clinical investigations,experiences and proven cases,this paper presented the main directions and methods in the study of Guo-Min (GM) decoction.It proved that GM decoction had identified anti-inflammatory and anti-allergic effects.Its effect may be through blocking H1 receptors,and lowering IgE in serum of type I allergic reaction to achieve.It showed that the clinical observation on multi-center,large sample randomized double-blinded controlled trials combined with compatibility laws of Chinese herbs and prescriptions by chemomics will be the future study direction of GM decoction.It played significant role in the prevention and treatment of allergic diseases.

Objective Post-operative hypoxemia occurred frequently after extubation in patients who underwent orthotopic liver transplantation (OLT) in ICU. This study is to investigate the efficiency of hyperoxia solution in improving oxygenation. Methods Ten OLT cases, who post-operatively manifested hypoxemia after extubation, were enrolled in this study. Hyperoxia Ringer's solution (500ml) prepared by Jing-Shu-Yang (an apparatus of oxygen supply, Vanform Co., Shandong) was infused at a rate of 200ml/hr. Hemodynamic parameters, blood gas, and blood lactate were determined at the beginning, end of infusion, and 1 hour after the infusion, with recording of the following data: heart rate (HR), mean arterial pressure (MAP), pulmonary arterial pressure (PAP), and pulmonary capillaries wedged pressure (PCWP). Results Compared with the levels at the beginning of infusion, SpO2 and SvO2 increased significantly at the end of the infusion of 500 ml hyperoxia Ringer's solution, and they returned to the baselines 1 hour later, although regular Ringer′s solution was coutinuously given by the same infusion rate. HR, MAP and PCWP were kept stable with the solution infusion. Notably, PAP was decreased markedly at the end of infusion, and blood lactate level remained unchanged after the intervention. Conclusion Hyperoxia Ringer's solution infusion improved oxygenation in OLT patients who suffered from acute lung injury with hypoxemia postoperatively. PAP decreased markedly as hypoxemia was corrected.

Objective To explore a way that induce human bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro.Methods Bone marrow mesenchymal stem cells were cultivated in a medium containing serum by using density gradient centrifugation method and whole marrow method and were induced to differentiation by ?mercaptoethanol.Immunohistochemistry were used to identify the type of cells.Results The number of cells was((1.73)?(0.44))?10~(7)/ml by using whole marrow method and ((7.65)?(0.52))?10~(7)/ml by using density gradient centrifugation method at the 10th day cultivation respectively.There was statistically significant difference(P

AIM:To determine terpenelactonesines content in Yinxing Tongzhi Dripping Pills(ginkgoflavone,ginkgolide).METHODS:HPIC with evaporative light scattering detector(ELSD) was established to determine terpenelactones content in Yinxing Tongzhi Dripping Pills..The analysis was carried out on Angilent Hypersil ODS(5 ?m,4 mm?250 mm) column.The mobile phase consisted of THF-MeOH-H_2O(10:20:70).The operating conditions of the ELSD were a nebulizer nitrogen,flow-rate of 2.51 L/min and an evaporator tube temperature of 103(?C).RESULTS:No interference was found in the terpenelactones peak position.The method was simple,with a good linear relationship.The relative standard deviation of reproducibility about BB、GA、GB、GC was 1.33%,(1.79%,)1.92%,1.58% respectively and the average recoveries were 97.0%,96.5%,96.3%,97.6%,respectively.CONCLUSION:The method can be used for quality control.