1.Investigation on Construction Situation of Pharmacy Staff Team in 22 Hospitals from Hubei Wuling Moun-tainous Area
China Pharmacy 2015;26(31):4462-4464
OBJECTIVE:To investigate the status quo and problems of the construction of pharmacy staff team from Hubei Wuling mountainous area,and to provide reference for the formulation of construction plan of pharmacy staff team. METHODS:Using questionnaire survey and telephone interviews,the pharmacy staff of 22 hospitals at second level or above from Hubei Wul-ing mountainous area during 2011-2014 were investigated in terms of number,age,educational background,professional title,etc. RESULTS & CONCLUSIONS:The shortage of pharmacy staff is seious in 22 hospitals at second level or above from this areas (open 10 606 beds,should be staffed 956 pharmacy personnel,actual number of pharmacy personnel is 502);the age structure is unreasonable,and only 220 pharmacy staff aged under 35 year-old;highly educated pharmacy staff accounted for low proportion, and bachelor degree or above took up 29.5%;pharmacy staff with professional title took up low proportion,and pharmacy staff with high,middle and junior professional titles accounted for 1:5:4. The situation of pharmacy staff in the region can be improved through following measures,including striving for policy recommendations,continuously enriching the quantity of pharmacy staff, integrating scientifically and building reasonable talent tem gradually,introducing talents,optimizing education structure of pharma-cy staff,strengthening the training,improving operation level of pharmacy staff,etc.
2.Correlation research between Gene Polymorphism of TNF-alpha Gene 863C_A and Thyroid Associated Ophtalmopathy in Zhuang Ethnic Population
Chinese Journal of Primary Medicine and Pharmacy 2013;20(1):18-20
Objective To explore correlation between gene polymorphism of TNF-alpha and thyroid associated ophtalmopa thy (TAO) in Zhuang ethnic population.Methods DNA and serum samples were collected in patients with TAO in Han/Zhuang population.ELISA was applied to detect level of TNF-alpha in serum and PCR was applied to detect polymorphism of TNF-alpha gene 863C_A.Results The levels of TNF-alpha in patients with TAO increased significantly compared with control group (P < 0.05),and more significantly in Zhuang population compared with Han population (P < 0.05) ;gene frequency of CA was significantly higher in patients with TAO in Zhuang population than that in Han population (P < 0.05),while gene frequency of AA was significantly lower (x2 =0.770,2.145,all P < 0.05).Conclusion Serum level of TNF-alpha increases during occurrence of TAO,and the differences of expression level between different nationalities are due to gene polymorphism of TNF-alpha.
3.Advances in the Research of Chlorogenic Acid and Its Extraction and Isolation Methods
Chinese Traditional Patent Medicine 1992;0(02):-
The structure, pharmaceutical functions and extraction and isolation methods of chlorgenic acid were reviewed to provide references for the research of chlorogenic acids and the production of the medicines chlorogenic acids.
4.Determination of imperatorin and isoimperatorin in Tongketing Capsules by HPLC
Kai MA ; Wenjie QIN ;
Chinese Traditional Patent Medicine 1992;0(10):-
Objective: To establish the determination of imperatorin and isoimperatorin in Tongketing Capsule (Radix Angelicae Dahuricae, Pheretima, etc.) by HPLC. Methods: The determination was carried out by HPLC. The mobile phase was acetonitrile water (60∶40) and UV detection wavelength was set at 245nm . The flow rate was 1.0mL?min -1 . The column temperature was 40℃ . Results: There were the good linear relationship between the absorption area value and the concentration in the range of 65?g~325?g for imperatorin and in the range of 50?g~250?g for isoimperatorin, respectively. The average recoveries were 98.3% ( RSD = 1.73% ) and 98.7% ( RSD = 1.65% ), respectively. Conclusion: The method is simple, accurate and can be used to determine imperatorin and isoimperatorin in Tongketing Capsule.
5.Advances in research on enterovirus 71 vaccines
Jie MA ; Yongqiang DENG ; Chengfeng QIN ; Ede QIN
Military Medical Sciences 2015;(7):553-556
Enterovirus 71 (EV71) is one of the primary causative agents of hand, foot and mouth disease in children and closely associated with severe neurological complications and even deaths.EV71 outbreaks have occurred throughout the Asia-Pacific region since 1990s, posing global public health threat;however, no specific therapeutic strategy exists for severe EV71 infection.Several inactivated vaccine products have entered or finished the clinical trial stage, and some novel vaccine candidates, including live attenuated, subunit, and virus-like particle, show great potential for further develop-ment.This review summarizes the present situation and progress in the development of EV71 vaccines.
6.Hemagglutinin A of Porphyromonas gingivalis function as adhesion and invasion in human gingival epithelial cells
Hong SONG ; Qin MA ; Yongjin CHEN
Journal of Practical Stomatology 2000;0(05):-
Objective: To determine if the hemagglutinin A (HagA) of Porphyromonas gingivalis could be involved in the adhesion and invasion in human gingival epithelial cells (HGEC). Methods:P. gingivalis 381 hagA mutant was constructed by conjugation method. The whole length of hagA gene was cloned into pYA292 in Salmonella typhimurium x4072 (S. typhimurium-hagA). The strains were used to test their ability of adhesion and invasion into HGEC using a standard antibiotic protection assay. S. typhimurium x4072 strains containing empty vectors were used as negative control. HagA expression in S. typhimurium-hagA was confirmed by Western blot. Results:Although there were no significant differences between P. gingivalis 381 hagA mutant and wild type in adhesion and invasion into HGEC, the adhesion values of S. typhimurium-hagA to HGEC were increased by 3 times compared to their respective controls, while the invasion ability of S. typhimurium-hagA was 4 times greater than that of the negative controls. Conclusion: These results suggest that HagA may participate in P. gingivalis adhesion and invasion into HGEC.
7.Effect of scenario simulation on communication skills
Li MA ; Zhixian LUO ; Qin ZHOU
Modern Clinical Nursing 2015;(6):65-68
Objective To evaluate the effect of scene simulation on improving nurses′communication ability in the emergency department. Method Twenty nurses from the emergency department were trained with the scenario simulation. A comparison was done between the pre- and post-training abilities in terms of their ability in communication and the satisfaction degree of patients. Results The total score scores in every dementions on nurses′communication ability after training was significantly higher than those before training(all P<0.05). The score on satisfaction degree with nurses after training was significantly increased compared with that of pre-training and satisfaction degree of patients (all P < 0.05). Conclusion Scenario simulation training is helpful for the improvement of nurses′communication ability.
8.Modern Research Progress on Guo-Min Decoction
Lilan QIN ; Yuying LAN ; Shuran MA
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(11):2394-2397
By reviewing its experimental studies as well as clinical investigations,experiences and proven cases,this paper presented the main directions and methods in the study of Guo-Min (GM) decoction.It proved that GM decoction had identified anti-inflammatory and anti-allergic effects.Its effect may be through blocking H1 receptors,and lowering IgE in serum of type I allergic reaction to achieve.It showed that the clinical observation on multi-center,large sample randomized double-blinded controlled trials combined with compatibility laws of Chinese herbs and prescriptions by chemomics will be the future study direction of GM decoction.It played significant role in the prevention and treatment of allergic diseases.
9.Research progress of induced pluripotent stem cells and its bottleneck
Chinese Journal of Tissue Engineering Research 2014;(45):7358-7363
BACKGROUND:With the development of the research, induced pluripotent stem cel s are applied to the build of disease model, drug screening, regenerative medicine, and many other research fields, and have made significant achievements, especial y in the study of nervous system diseases.
OBJECTIVE:To summarize the recent development of induced pluripotent stem cel s and to raise problems and prospects based on the latest research in this field.
METHODS:The first author searched the PubMed database for articles about the induced pluripotent stem cel s, including reviews, clinical research and basic research, published from January 2006 to September 2014. The keywords were“iPS, induced pluripotent stem cel”, and final y 60 articles were included in result analysis.
RESULTS AND CONCLUSION:Induced pluripotent stem cel research continues to make breakthrough from its discovery by Yamanaka’s team in 2006 to winning Nobel Prize in 2012. Induced pluripotent stem cel research has broad prospects in the disease model construction, drug screening and regenerative medicine. Currently, problems such as reprogramming methods, cel stability, and clinical transformation stil need to be solved, and further researches are necessary.
10.STUDY ON THE ASSOCIATION BETWEEN A NOVEL CORONAVIRUS AND THE SARS PATHOGEN
Qingyu ZHU ; Ede QIN ; Ma YU
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
Objective To determine the causative association between a newly isolated coronavirus and the current epidemic of severe acute respiratory syndrome (SARS) Methods Coronavirus was isolated from the samples of patients with SARS by cell culture Immunofluorescence assay and neutralization test were used to detect the antibodies in serum of SARS patients against newly isolated coronavirus, in order to analyze and determine the association between coronavirus and SARS pathogen Results The antibodies against the novel coronavirus could be detected in 99 of 113 sera from clinically diagnosed SARS patients The results of 10 pairs of double serum detection showed that, antibody titers in the late phase were significantly higher than those in the acute phase, and a highest increase by 128 folds was being found. The neutralization test showed that the antibodies from SARS patients' sera could neutralize the novel coronavirus Conclusion The newly isolated coronavirus was closely associated with and possibly the key pathogen of SARS