Objective:To investigate dopaminergic neuron injury and ?-synuclein expression of mouse exposed chronically to low dose of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP). Methods:The C57BLmice received bake hypodermic injections of 25 mg/kg MPTP twice a week for 5 consecutive weeks. Abnormal behavior was observed during the treatment by using herbicide. The expression of throsine hydroxylase(TH)and ?-synuclein was determined by immunohistochemistry. The dopaminergic neurons apoptosis was determined by TdT-mediatd dUTP-biotin nick end labeling (TUNEL). Results:As compared with the controls of mice,those treated with MPTP displayed markedly hypoactive behavior. Immunohistochemical staining showed that the numbers of TH stain cells was decreased significantly after treatment with MPTP,and the density of ?-synuclein stain was increased significantly after treatment with MPTP. Apoptosis displayed after treatment with MPTP. Conclusion:Chronical injection with MPTP might induce abnormal behavior. apoptosis and continual aggregation of ?-synuclein in dopaminergic neurons of substantial nigra.

Objective To investigate the relationship between PrfA-dependent promoters and PrfA regulation. Methods LacZ reporter gene fusions used to investigate the inhibitory elements for PrfA-dependent transcription were carried on two promoters of Listeria monocytogenes: a PrfA-dependent promoter of the phospholipase gene pica (PplcA) and a putative promoter of the aroA gene (ParoA2) which contains a similar PrfA-binding site (PrfA-box) and a similar-10 box as PplcA but does not function as PrfA-dependent promoter. A series of hybrid plcA-aroA promoters by exchanging corresponding sequence elements of these two "promoters" were constructed and incorporated into upstream of a promoterless lacZ gene. The variant promoter-lacZ transcriptional fusions were then electroporated into L. monocytogenes wild-type strain P14, prfA mutant P14a and prfA deletion mutant A42, respectively. The expression level of PrfA is the highest in the P14a and the lowest in A42. The corresponding transcription activities of hybrid promoters were measured by the β-galactosidase assay. Results The two critical elements of PrfA-dependent promoters, the PrfA-box and the-10 box, can be functionally exchanged as long as the distance in between is maintained 22 or 23 bp. However, the interspace sequence and the sequence downstream of the -10 box of ParoA2 were strongly inhibitory for PrfA-dependent transcription. Conclusion Downstream sequence together -10 box of ParoA2 might fold into a hairpin structure when present in a single stranded DNA and possibly block the formation of the transcriptional initiation open complex, hence, inhibit the PrfA-dependent transcription from ParoA2.

Henoch Sch?nlein purpura( HSP) is a vasculitis of primary systemic vascular lesions of aller-gic diseases in children. Henoch Schonlein purpura nephritis( HSPN) is the most common allergic purpura sec-ondary renal disease,hematuria and(or)proteinuria of allergic in purpura the course(the most six months) can make diagnosis. The clinical manifestations varyies in severity,and the incidence has increased year by year,the incidence may be related to infections, allergies, immune abnormalities, immune damage and so on. So raising awareness of children nephritis,early diagnosis,early treatment has a great impact on the prognosis of children.

ObjectiveTo investigate the teaching effect of problem-based learning(PBL) in clinical probation of gynecology and obstetrics.Methods48 students who majored in obstetrics and took internship in Xiamen Maternity and Child Care Hospital were selected as study subjects and were randomly assigned to control group and observation group.Traditional teaching pattern and PBL were performed in the above-mentioned groups respectively.The score of final test on maternity nursing and self-evaluation on capacity advancement were compared between two groups.ResultsThe score of observation group were significantly higher than control group (P＜0.05); Similarly,the self-evaluation of observation group after internship was also elevated with significance (P＜0.05) when being compared with control group.ConclusionEvidence showed that PBL was capable of improving the knowledge of maternity nursing,and it was also been proved effective in fostering the ability of self-learning,communication,and solving problem.Therefore,the PBL was appropriate to be promoted in the internship of nursing education.

Insulin receptor substrate(IRS)-1 and IRS-2 expression levels of liver tissues and skeletal muscle in intrauterine growth retarded(IUGR)rats were investigated by RT-PCR and immunohistochemistry.An IUGR animal model was established by maternal nutrition restriction during pregnancy.IRS-2 expression level of liver tissue and IRS-1 expression level of skeletal muscle in IUGR rats at 0 and 3 weeks old were significantly lower than those in normal rats at the same age respectively,and insulin resistance was induced in IUGR,and these findings might be the molecular mechanisms susceptible to metabolic syndrome in IUGR rats.

Objective To explore the application of scene type trace method in physiology teach-ing reform. Methods 2009 grade clinical medicine students in Zunyi Medical College were randomly di-vided into two groups:control group (n=111) and experiment group (n=108). Students in control group were taught by traditional teaching method while those in experimented group by scene type trace method. According to the questionnaire survey and the final exam statistical analysis,the effects of teach-ing were evaluated. SPSS 15.0 software was used to do statistical analysis. Qualitative data were analyzed by chi-square test of completely randomized design and two sets of frequency distribution while quantita-tive data by independent samples t test. P<0.05 means that difference is statistically significant. Results There were statistically differences in satisfaction degree of two teaching methods. More students were sat-isfied with scene type trace method than traditional teaching method. Final exam achievement in scene type trace method group(79.90±14.25,90.74%)were higher than that in the traditional teaching method group(72.35±17.09,83.78%). Conclusions Physiology teaching scene type trace method is helpful to improve the students' learning initiative and significantly improve students' learning achieve-ment.

Objective To investigate the clinical value of endoscopic ultrasonography (EUS)on minute gas-tric stromal tumor (MGST).Methods 90 patients diagnosed with MGST were randomly divided into two groups. 45 patients in A group were given conventional endoscopic diagnosis,45 cases in B group were treated with endoscopic ultrasound diagnosis.After the two groups of patients with endoscopic treatment,the coincidence rate of preoperative diagnosis and pathological inspection test results was recorded,clinical features in MGST under EUS diagnosis were analyzed.Results All patients were diagnosed in the case of endoscopic surgery,endoscopic submucosal dissection (ESD)accounted for 94.44%,which was significantly higher than the use of endoscopic full -thickness resection (EFR)percentage of 5.56%,and the difference was statistically significant (χ2 =14.22,P <0.05);The two groups of patients after endoscopic treatment,were diagnosed as pathological inspection MGST.Preoperative diagnosis showed that EUS MGST suspected cases of 40 patients,conventional endoscopy MGST suspected cases of 31 cases.The diag-nosis coincidence rate of B group (88.89%)was significantly higher than that in A group (68.88%),the difference was statistically significant (χ2 =5.40,P <0.05);MGST of EUS images could be clearly observed in patients with tumor morphology,size,location,growth pattern,internal parenchyma,echo pattern,strengthening methods,the compo-sition ratio in line with the results of endoscopic treatment,diagnosis results in line with the pathological diagnosis. Conclusion EUS has significant value for the diagnosis of MGST,it has advantages in the clinical guidance and treatment.

Objective　To study the causes of failure to IOL implantation during cataract surgery.Methods　We reviewed 540 eyes in which 23 eyes were failed to implant IOL during cataract surgery.Results　Detachment of suspended zonular ligament, rupture of posterior capsule and vitreous prolapse are the main factors influencing IOL implantation during cataract surgery.Conclusion　Improvement of surgical technique and reduction of complications are the key points for the IOL implantation successfully during cataract surgery.

Objective To investigate the expression of human telomerase reverse transcriptase(hTERT),Ki-67 and p27~(kip1) in pheochromocytoma and paraganglioma,and to evaluate whether the expression of hTERT,Ki-67 and p27~(kip1) could serve as diagnostic markers for predicting the biological behaviour of these tumours.Methods Expression of hTERT,Ki-67 and p27~(kip1) was determined by immunohistochemistry in 45 pheochromocytomas/paragangliomas(31 benign,7 suspected malignant and 7 malignant)and 9 normal adrenal medulla samples.Results The hTERT was expressed in 5/7 malignant tumors,5/7 suspected malignant tumours and 3/31 benign tumours.Malignant and suspected malignant tumors expressed more hTERT(P

OBJECTIVE:To simultaneously determine the contents of CIPRO and MNZ in compound ciprofloxacin ear dro_ ps by nodical polyploid UV-spectrophotometry.METHODS:CIPRO and MNZ were nodical absorption at wavelength of350.0nm and this was just the sum of isoabsorptive point of two components while DXM did not absorb UV ray at this point.Therefore350.0nm was adopted as the detecting wavelength for these two components.RESULTS:The average recovery and relative standard deviation of CIPRO were99.7%and1.4%respectively.The average recovery and relative standard de?viation of MNZ were100.7%and1.6%respectively.CONCLUSION:The method is accurate,rapid,simple and sensitive.This method can be used to control the quality of this preparation.