1.Applications of FRET technology in the study of mechanotransduction.
Journal of Biomedical Engineering 2013;30(6):1362-1367
Mechanical force has essential effects on cellular behaviors such as proliferation, migration and differentiation, and the mechanism of mechanotransduction is still one of the hot spots in mechanobiology study. Traditional methods could not provide accurate evaluation of the protein activation signal upon mechanical stress application. The development of fluorescence protein technology greatly promoted the understanding of mechanotransduction. In particular, genetically-encoded biosensors based on fluorescence resonance energy transfer (FRET) technique has achieved a real-time dynamic observation of living cell signal protein activity, which provides a powerful tool for the in-depth study of biomechanics. In this paper, we provide a summary on recent progress of FRET application in biomechanics. Firstly we introduce the FRET technology, and then we summarize three methods to integrate the mechanical stimulation with the FRET imaging system on cell experiments. After that, the important progress of biomechanical research on signal pathway made by FRET technology, such as cytoskeleton, Rho family, calcium and cellular physical stress visualization, are also discussed. Finally, we point out the bottleneck of the future development in FRET technology, and also make the prospect of the application of FRET in mechanotransduction. In summary, FRET technology provides a powerful tool for the studies of mechanotransduction, which will advance our systematic understanding on the molecular mechanisms about how cells respond to mechanical stimulation.
Biomechanical Phenomena
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Biosensing Techniques
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Fluorescence Resonance Energy Transfer
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Humans
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Luminescent Proteins
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chemistry
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Mechanotransduction, Cellular
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Microscopy, Fluorescence
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Signal Transduction
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Stress, Mechanical
2.Progress of standardized treatment in gastrointestinal surgery
Chinese Journal of Digestive Surgery 2012;11(1):19-21
With the change of diet and lifestyle,the development of medical concepts and techniques,the spectrum of disease has changed dramatically. The standardization of gastrointestinal surgery will be the key to further improvement.Endoscopic ultrasonography,computed tomography,magnetic resonance imaging, positron emission tomography-computed tomography and laparoscopy are helpful for preoperative staging of gastrointestinal carcinoma.The standards of operation,such as D2 lymph node dissection for gastric cancer and total mesenteric excision for rectal cancer,are also very important.Creating standards for multi-disciplinary treatment of gastrointestinal diseases will be important factors for improving outcomes.
3.Denture adhesive Protefix relieves the discomfort of patients with complete denture
Journal of Practical Stomatology 2015;(3):434-436
Denture adhesive(DA)Protefix was used in 31 edentulous patients with complete denture.NRS evaluation showed that applica-tion of Protefix decreased the uncomfortable score(P <0.05)at 2 h,1 d,7 d after use.Protefix improved denture retention,stability and masticatory function.
4.Analysis on main death causes and potential life lost among residents in Chongqing Wansheng Economic Development Zone during 2014
Chongqing Medicine 2016;45(18):2528-2531
Objective To understand the death level and main death causes among residents in the Wansheng Economic De‐velopment Zone to provide the basis for comprehensive prevention and control .Methods According to the ICD‐10 international classification of diseases ,the death causes were classified and coded for statistically analyzing the crude mortality rate ,standardized mortality rate and the death causes constituents in this surveyed population .Results The crude mortality rate of residents in Wan‐sheng Economic Development Zone during 2014 was 650 .57/100 000 and the standardized mortality rate was 413 .35/100 000 .The sequence of death causes was circulatory system disease ,respiratory system disease ,tumor ,injury and poisoning and digestive sys‐tem diseases .The death number for these 5 kinds of death causes accounted for 93 .21% of the total death number .The top five of potential life loss years were injury and poisoning ,tumor ,circulatory system disease ,respiratory system disease and digestive system diseases .The rates of potential years of life loss were 13 .76‰ ,10 .19‰ ,8 .35‰ ,4 .84‰ and 2 .69‰ respectively .Conclusion Chronic noncommunicable diseases ,injury and poisoning become the main causes of death in the Wansheng Economic Development Zone and disease burden .
5.Logistic regression analysis on fatal risk factors to patients with sepsis
Medical Journal of Chinese People's Liberation Army 2001;0(08):-
Objective To study the fatal risk factors to the patients with sepsis.Methods The data of 49 patients with sepsis were analyzed,who were consecutively admitted to intensive care unit(ICU)of Peking Union Medical College Hospital within six months.Patients' general states,clinical information,human leucocyte antigen-DR(HLA-DR)in blood monocytes and plasma level of diamine oxidase(DAO)were recorded.Simple logistic regression was done to analyze all the risk factors,and statistically significant variables were selected and used in multivariate and unconditioned Logistic regression analysis.The primary end point was hospital mortality.Results The subjects involved in present study were consisted of 49 patients with sepsis admitted to ICU of the Peking Union Medical College Hospital from Aug.2003 to Jan.2004,averagely aged 60.8?16.9 years;including 32 males(65.3%)and 17 females(34.7%);with APACHEⅡscores 18.6?8.0 and SOFA scores 5.9?3.8.Twenty-one out of the 49 patients(42.9%)were from non-operation department,and the other 28 patients(57.1%)were from operation department.ICU duration was 14.4?12.8 days;eighteen of the 49 septic patients died during hospitalization,with the 28-days mortality of 36.7%.Positive blood cultures(OR=5.237,95%CI 1.041~26.34,P=0.045),decreased platelet count and blood HLA-DR(OR=0.943,95%CI 0.901~0.98,P=0.01),and increased plasma levels of DAO(OR=2.235,95%CI 1.101~4.53,P=0.026)were obviously associated with mortality.The positive blood cultures,decreased blood HLA-DR and increased plasma levels of DAO were independent fatal risk factors for the patients with sepsis.Conclusions The positive blood cultures,decreased blood HLA-DR and increased plasma levels of DAO are high risk for the patients with sepsis at admission.
6.Cryopreservation of test-tube shoot tips of Siraitia grosvenorii by vitrification and plant regeneration
Chinese Traditional and Herbal Drugs 1994;0(02):-
Objective To get a new approach for conserving the germplasm of Siraitia grosvenorii Methods Shoots, 0.8—1 cm in length, excised from test-tube plantlets which were subcultured 15 d, were precultured. Then its shoot-tips about 2—3 mm in length were dissected and loaded with 60% PVS2 at 25 ℃, and dehydrated with 100% PVS2 at 0 ℃, changed for fresh 100% PVS2 prior to directly plunging into liquid nitrogen. After cryopreservation for 24 h, the shoot tips were thawed, rinsed in MS+1.2 mol/L sucrose medium, blotted with filter papers, then plated on the MS + 1.0 mg/L 6-BA+0.05 mg/L NAA + 0.1 mg/LGA3 + 0.8% agar + 45 g/L sucrose medium at 25 ℃ for 7 d in dark prior to exposure to the light. The root medium for regeneration plantlets was 1/2 MS + 0.2 mg/L NAA + 30 g/L sucrose. Results Shoots were precultured for 3 d on MS + 0.7 mol/L sucrose medium, then its shoot-tips loaded for 40 min, dehydrated for 50 min. After cryopreservation, the shoot tips were rapidly thawed in water at 40 ℃, then rinsed for 40 min. The survival rate of shoot-tips plated on the recovering medium in one week was 100%, and regeneration rate after 30 d was 78.33%, which was the highest. The regeneration plantlets inoculated on root medium were reconstructed integrating plantlets. Conclusion The method of vitrification to cryopreserve the germplasm of S. grosvenorii is a simple way with high survival rate and normal regeneration plants.
7.STUDIES ON THE EFFECTIVE COMPONENTS IN PROTECTIVE SEKA AGAINST PLASMODIUM YOELII INFECTIONS IN MICE
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(02):-
Sera were collccted from mice immunized separately with schizonts, merozoitcs or different parasite stages, or from mice which had been recovered from Plasmodium yoelii yoelii infection. The effect of protection of cach serum against P. y. infection was assayed by passivc transfer technique. It was shown that only sera collected from mice which either had been trcated with chloroquine or had been naturally recovcred from P. y. infections can transfer certain dcgree of resistance to normal mice. The cffectivc sera delayed the appearance and the peak of parasitemia, and prolonged the survival time of the challenged mice. After these sera were fractionatcd by 50%(NH4)2SO4 precipitation, the prccipitated fraction showed similar degree of protection as the non-fractionated sera. Chloroquine-treated serum was shown to be able to inhibit the invasion of new erythrocytes by marozoite in vitro. 35S-methionine-labelcd antigens were precipitated by protective or non-protective sera and the bands were compared on SDS-PAGE and autoradiogram. Since thc antigcn bands of 245, 210, 190, 156 and 130 KD can only bc precipitated by protective sera but not by non-protectivc sera, it is possible that the protection of chloroquine-treated serum is induced by the specific antibodies against the above antigens.
8.THE EFFECT OF INHIBITOR VARIATIONS ON THE GROWTH OF BACILLUS LICHENIFORMIS
Microbiology 1992;0(05):-
This paper reports reaction of Bacillus lichendermis on the inhibitor vacations. The result Shows that, inhibition of the 500?g/mL of cephalosporin 1000?g/mL of penicillin, 500?g/mL of streptimycin, on the Bacillus Licheniformis,Whe the Phis 7.0. This provides a sicentific basis for using the preparation of Bacillus licheniformis.
9.The effect of glutamine on the nutrition metabolism and intestinal mucous barrier in MODS patients
Parenteral & Enteral Nutrition 2004;0(05):-
Objectives: To evaluate the effects of glutamine on the nutritional metabolism and permeability of intestinal mucosa in MODS patients.Methods: The randomized and controlled study was designed.Twenty MODS patients were randomly divided into control group and treatment group.The concentration of serum albumin,transferrin and blood sugar,L(lactulose) and M(mannitol) ratio in urine,nitrogen balance,the related complication were compared and obserwed.Results: In treatment group,the concentration of serum albumin,prealbumin and transferrin were higher than that of control group on the same time and showed significant difference(P
10.Carotid artery stenting for carotid artery stenosis
Journal of Medical Postgraduates 2003;0(10):-
Carotid artery stenting (CAS) for treating carotid artery stenosis is a recent developed technique which is less invasive and complications. This paper reviewed the progress, procedure, and complications of carotid artery stenting.