Aim In order to detect Mycoplasma fermentans (Mr)and Mycoplasma penetrans (Mpe) at the same time dual nested-PCR(DN-PCR) technique was established Method Mpe the same time PCR Primers were designed according to published 16s rRNA Sequences of Mf and Mpe, the outer primers were univevsal to both Mf and Mpe and the inner was Mf、Mpe speciespecific respectively. Two-step amplification procedures were perfomed. Result Chromo some DNAS of Mf and Mpe gave characteristic DN-PCR prafiles which were differeht from other my coplasmas or microorganism tested.Conclusion The DN-PCR method was established for the detection of Mf and Mpe at the same time which was sensitive specific and rapid.

Objective To study the influence of different dosages of atorvastatin in acute coronary syndrome female patients on vascular endothelial growth factor(VEGF) and brain natriuretic peptide(BNP).Methods 60 female cases of ACS patients were divided into A and B group.30 patients in the A group were given atorvastatin 20mg per night,while the other 30 patients in the B group were taken 40mg per night.The patients were followed four weeks by evaluating serum VEGF and BNP concentration.Results After treatment for four weeks,the patients in the two groups showed significant reduction in VEGF and BNP,however,the therapeutic group showed significant difference between the two groups.Additionally,the B group further showed significant reduction in VEGF and BNP.Conclusion Using high-dose atorvastatin lowers VEGF and BNP significantly,which could reduce female cardiovascμlar events.

BACKGROUND:Traditional Chinese medicine glossoscopy observes tongue image by vision,and to diagnose diseases by physicians'clinical experiences,which lacks of objective diagnostic methods and criteria;finally resulting in affecting the effectiveness of this diagnostic method.Therefore,it is necessary to realize quantitive and objective glossoscopy using techniques such as computer vision.Tongue body segmentation is the premise of the tongue image recognition diagnostic system.The quality of segmentation directly affects the sequent work.At present,there are many methods of tongue body segmentation,but obtained results cannot ensure the accuracy,and the anti-interference ability is poor.OBJECTIVE:To design a new method of tongue image segmentation,and effectively isolate tongue body.METHODS:According to tongue-images gray and color's features,a segmentation method of combining gray projection and threshold-adaptive way is presented in this paper.By this method,the intensity image of a colorful tongue-image is firstly projected at horizontal and vertical direction to locate the tongue-body area,and then threshold-adaptive way Otsu is used to segment the located area.RESULTS AND CONCLUSION:Tongue-body segmentation is the basis of automatic tongue-images analysis.The following 281 tongue-images segmentation experiments show the efficiency of this method.

The myelodysplastic syndrome(MDS) comprise a heterogeneous group of clonal disorders.A unique aspect of MDS is the notion that both bone marrow failure and the propensity toward development of acute leukemia coexist. Origin of abnormal clone with MDS occurs at the level of multipotent progenitors or above it. Recently some kinds of MDS were observed the abnormal clone has been potentially originated from normal CD+34 CD-38 Thy-1 + hematopoietic stem cells. The abnormal clones with different chromosomal abnormality have different distribution. With the propotion of abnormal clonality increasing MDS has aggravated. Coherent result will provide new insight into the clinical diagnosis, treatment and prognosis of MDS by strengthening the research of MDS clonality.

With the advancement in computer and imaging technologies, more and more surgical procedures can be performed with the help of the computer. This is especially true with the orthopaedic trauma surgery where the surgeons are working indirectly on the images, either acquired preoperatively or intraoperatively, to carry out fracture fixation procedures. The images can come from CT scan, intraoperative real-time fluoroscopy or endoscopy. The combination of computer and other information technologies in clinical practice is sure to be one of the major developments in the coming years. In this issue of the Chinese Journal of Orthopaedic Trauma, we will update you with the clinical applications of the real-time fluoro-navigation.

OBJECTIVE To investigate mechanisms of drug resistance in multidrug-resistant strains of Acinetobacter baumannii(MDR-ABA).METHODS The resistant genes related to 5 kinds of antibiotics were detected by using polymerase chain reaction and verified by DNA sequencing.RESULTS In 30 strains of MDR-ABA,the positive rates of TEM,ADC,armA,aac(3)-Ⅰ,ant(3″)-Ⅰ,dfrA12 and sul1 were 63.3%,70.0%,30.0%,90.0%,90.0%,53.3% and 80.0%,respectively.CONCLUSIONS There are high positive percentages of TEM,ADC,armA,aac(3)-Ⅰ,ant(3″)-Ⅰ,dfrA12 and sul1 genes in MDR-ABA.

OBJECTIVE To investigate mechanisms of aminoglycoside resistance in pan-drug resistant Pseudomonas aeruginosa(PDRPA).METHODS The 11 genes of 16S rRNA methylase and aminoglycoside-modifying enzymes were detected by polymerase chain reaction(PCR) and verified by DNA sequencing.RESULTS In 33 strains of PDRPA,the positive rates of rmtB,aac(3)-Ⅱ,aac(6′)-Ⅰb,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ were 42.4%,51.5%,42.4%,57.6%,48.5% and 63.6%.A total of 32 strains identified aminoglycoside modified genes,one strain aminoglycoside-modifying enzyme gene did not discovered,but rmtB positive.CONCLUSIONS The aminoglycoside resistance mechanisms of the PDRPA are the production of 16S rRNA methylase and aminoglycoside-modifying enzymes related.

To investigate the change and clinical significance of interleukin(IL)-23 and IL-17 levels preliminarily in peripheral blood of patients with obstructive sleep apnea-hypopnea syndrome(OSAHS).The levels of IL-23 and IL-17 in serum and culture supernatants of peripheral blood mononuclear cells(PBMCs)were determined by ELISA in patients with OSAHS and normal volunteers.To observe the changes of IL-23 and IL-17 levels before and after the treatment of Han-uvulopalatopharyngoplasty(H-UPPP) in patients with OSAHS.The levels of IL-23 and IL-17 in serum and culture supernatants of PBMCs in patients with OSAHS were significantly higher than those in normal control group(P<0.05),and the levels of serum IL-23 and IL-17 were increased with the disease severity.The levels of IL-23 presented positively correlated with those of IL-17 in both serum and culture supernatants of PBMCs(rs=0.644,P<0.01;rs=0.251,P<0.05).After two months later of H-UPPP surgery, the levels of serum IL-23 and IL-17 were significantly lower than that before the treatment(P<0.05)as well as the apnea-hypopnea index.The sleep quality and low oxygen was improved obviously, and the lowest oxyhemoglobin saturation elevated significantly(P<0.05).IL-23 and IL-17 may play an important role in pathogenesis and prognosisof OSAHS.

Objective To study the effect of Danshen injection on brain peptide type B (NT‐proBNP) in patients with hyperten‐sion and left ventricular failure ,provide an effective basis for clinical treatment .Methods Totally 80 patients of hypertension with left heart failure from February 2015 to February 2016 were randomly divided into two groups :40 patients were treated with con‐ventional therapy (control group ) ,40 patients in the conventional treatment combined with Danshen injection (experimental group) .We compared NT‐proBNP ,inflammatory factors and clinical efficacy between two groups .Results Before treatment ,the expression of NT‐proBNP and hs‐CRP ,NT‐proBNP level was basically the same in two groups (P> 0 .05) .After treatment ,the ex‐perimental group of patients with NT‐proBNP was significantly lower than the control group and the LVEF ,left ventricular mass index and left ventricular volume index were significantly higher compared with the control group (P< 0 .05) .The total effective rate of patients in Danshen injection groups was significantly higher (P< 0 .05) .Conclusion The injection of Danshen in patients with hypertension and heart failure patients can significantly improving NT‐proBNP levels and improve the cardiac function .

Attention deficit hyperactivity disorder (ADHD) is the medical treatment, while it does not bring high compliance.Therefore, non-medical therapy is a research focus in this field.Non-invasive neuromodulation can regulate the functional state of central nervous system by electric, magnetic, or chemical approach through invasive equipment that is proved to be highly safe.In the clinical industry, non-invasive neuromodulation is a widely used physiotherapy in various types of neuropsychiatric disorders, and has received satisfying effects.On the contrary, the utilization of this technique on ADHD in clinical settings is still under the research stage.This review aims at elaborating the clinical progress of several non-invasive neuromodulation approaches on ADHD, so as to provide reference to future researches in this field.