Magnetic resonance imaging (MRI),a commonly used imaging technique,has been extensively investigated in lung cancer diagnosis and staging.Many studies have demonstrated that MRI can be used for the differential diagnosis of benign and malignant pulmonary nodules and for the screening of lung cancer; it also has important value in TNM staging of non-small cell lung cancer (NSCLC),with equivalent sensitivity and specificity to 18F-fluorodeoxyglucose (18F-FDG) PET-CT,which suggests that MRI can be used as an alternative imaging modality in noninvasive diagnosis and staging of NSCLC.

Objective:To evaluate the etiology,elinic characteristics,diagnosis and treatments of spontaneous perforation of the bile duct in children. Methods:The clinical data of eight children with spontaneous perforation of the bile duct between 1998 -2006 were analyzed retrospectively. Results:5 of 8 cases were diagnosed bile duct perforativn before operation,all the patients recovered favorably after T-tube and pelvic drainage. 3 children complicated with choledochal cyst were cured afterⅡstage radical operation. Conclusions:the etiology of spontaneous perforation of the bile duct in children is unclear, panereaticobiliary confluence malfunction (PBCM) maybe the main reason. Peritoneocentesis is very important to the diagnosis, which is difficult in child preoperatively. The effective surgical treatments are T-tube and pelvic drainage. The children complicated with PBCM needⅡstage radical operation,and long term follow up are necessary for the children present with spontaneous perforation of the bile duct without PBCM.

Objective:To investigate the effect of ultrasound on the ultrastructure and expression of P-gp of Neuroblastoma cell in order to discuss the mechanisms of ultrasound affecting the chemotherapeutic sensitivity of SK-N-SH cell. Methods:The SK-N-SH cells were divided into the experimental group and the control group. In the experimental group,the cell suspensions were exposed to ultrasound irradiation. In the control group,the cell suspensions were exposed to sham irradiation . The changes of ultrastructure of tumor cells were observed by scanning electron microscope and the expression of P-gp in two groups were detected. Results:(1) In the pictures took by scanning electron microscope,we found that: the configuration of tumor cell changed and there were some holes of different diameters on the cell membrane and the numbers of microvillus reduced or disappeared after ultrasound irradiation.(2) The result of immunocytochemisty showed the expression rate of P-gp in SK-N-SH cells in the control group was 56.23%?9.86% vs 34.86%?6.19% in the experimental group (P

Objective To study the ultrasonic characteristics of fetal cutaneous hemangioma and the association with perinatal outcomes.Methods Five fetuses with cutaneous hemangioma were detected by gray-scale and color Doppler ultrasound,compared with the result of pathology and hematology examinations after birth.Results Of the 5 cases diagnosed by ultrasound,3 had arteriovenous fistulas.In these 3 cases two fetuses developed high-output cardiac failure,and one developed cardiac insufficiency and thrombocytopenia.Finally one fetus was induced,one fetus died in the uterus and one neonate survived.The other two fetuses who had small tumors diagnosed by ultrasound did not develop any perinatal complications, and the neonates had favourable prognosis.Conclusions Massive hemangiomas are frequently associated with life-threatening complications such as high-output heart failure,thrombocytopenia and disseminated intravascular coagulopathy(Kasabach-Merritt syndrome).

Objective To summarize the perioperative nursing experience in nursing children with formaral angulation deformity treated with sreminobicular crib external fixator. Method The clinical data of 15 children with formaral angulation deformity treated with sreminobicular crib external fixator were reviewed. Results All 15 children got recovered, with the femora extended by 4~9 cm. After operation, all of them were affected by knee flexion dysfunction and foot drop to varied extents, which were improved satisfactorily after systematic training. Conclusions The systematic and progressive training can prevent knee flexion dysfunction and foot drop. Careful observation and effective nursing play an important role in the treatment of formaral angulation deformity.

Meckel′s diverticulum is the most common congenital anomaly of the gastrointestinal tract. Most ca-ses are asymptomatic,while it can be symptomatic when complications happened,its complications often expressed as hematochezia,abdominal pain,vomit,fever,and so on. When signs or symptoms arise from a Meckel′s diverticulum, morbidity and mortality will increase. So make a definite diagnosis of the diverticulum play an important role in the treatment of Meckel′s diverticulum. In this essay,now illustrate the meaning of imaging examination about the diagnosis of Meckel′s diverticulum,wish to make a diagnosis as soon as possible.

BACKGROUND: Osteoporosis has been one of the main reasons for old people' s in decline living standard and shortening of lifespan. The onset of osteoporosis is related to the loss of functional coupling in osteoblast and osteoclast. So observation of the regulative effect of medicines on osteoblast is a good method to evaluate their preventive and therapeutical efficacy in osteoporosis.OBJECTIVE: To investigate the effects of genistein on osteoblast culture in vitro.DESIGN: A randomined, controlled and single-blind study based on rat osteoblasts.SETTING: Department of laboratory in amunicipal hospital.MATERIALS: The experiment was completed in the Room of Isotope, Department of Nuclear Medicine, China-Japan Friendship Hospital of Beijing from February to December 2001. Ten SD rats of 24 hours old were provided from Beijing Weitong Lihua Experimental Animal Ltd. Co. (license:SCXK11-00-0008 ). The laboratory was SPF grade.METHODS: Skull osteoblasts of rats were selected to culture in vitro. Microculture tetrozolium, p-nitrophenyl phosphate, atomic absorptiometry and alizarin red were used to observe the effect of genistein on proliferation,activity of alkaline phosphatase(ALP), contents of matrix calcium and the number of mineral nodes of osteoblasts cultured in vitro.MAIN OUTCOME MEASURES: Morphological observation, determination of proliferation ratio, ALP stain, determination of matrix calcium accumulation and number of mineralized nodes with alizarin red stain.RESULTS: It was found that genistein stimulated the proliferation of osteoblasts, improved the ALP activity, and increased the contents of matrix calcium and the number of mineral nodes in cultured osteoblasts.CONCLUSION: Genistein has the effects on stimulating the proliferation,differentiation, maturation and mineralization of osteoblasts cultured in vitro.

Objective To investigate a superior therapeutic method of chloasma with traditional Chinese medicine (TCM) in women of reproductive age. Methods Two stage cross trial were employed in this study. Valuable labels were included pharmaceutical, periodical and layover effect, clinical symptoms, chloasma area, and correlation of them. Therapeutic course had 4 weeks in the first and the second stage, respectively. Clinic final effect was valued by intentional methodology. Results 106 recipients who fulfilled with inclusion criteria were divided into two groups, therapeutic group and controlling group, respectively. Effect rate of treating group arrived at 60.4% after and before the cross treatment. Therapeutic effect in the treating group was significantly superior to that in the controlling group in the end of clinic final. The pharmaceutical and the periodical effect also manifest markedly difference in this study. But there was not cross effect between the pharmaceutical and periodical effect in each group. The symptom effect has no relation with chloasma area effect, both of them also manifests line relation. Conclusion On the basis of pathological factors such as heat-damp, blood stasis and phlegm, harmonizing liver and reinforcing kidney is good way to improve therapeutic effect.

Objective To observe the effect of puerarin injection (PI) on insulin resistance in gestational diabetic mellitus(GDM) rats. Methods Rats with gestation for 5 days received onedose injection of STZ 35 mg/kg into subaortic caudal vein. After injection for 72 hours, rats with blood sugar over 13.5 mmol/L indicated the modeling being successful. Then the model rats were divided into treatment group (TG, using Puerarin), westernmedicine control group(WCG, using rosiglitazone) and model group (MG, using 0.9 % NaCl), 10 rats in each group .Ten normal gestational rats were used as normal control group (NCG, using 0.9 % NaCl).Two weeks later, the changes of blood sugar, Creactive protein (CRP) and tumor necrosis factor- ? (TNF- ? ) were observed. Results Compared with MG, the above indexes were improved in TG and WCG after treatment( P

Objective To investigate the proliferation rate of HepG2 cell after multiple thermotherapy and the possible reasons related to it. Methods After HepG2 cell were treaded by ten repeated cycles of heat exposure at 43 ℃ for 80 minutes twice a day, the doubling time of cell was analyzed, and the cell cycle, bcl-2 mRNA and bax mRNA were detected. Results The proliferation rate of HepG2 cell which treated with heat speeded up, the percentage of G2 and S in cell cycle increased, the expression of bcl-2 mRNA strengthened and the rate of bcl-2/bax increased. Conclusion The speeded proliferation of HepG2 cell after multiple thermotherapy is related to its high percentage of DNA duplicated and dividing cell, strengthened expression of bcl-2 mRNA and increased rate of bcl-2/bax.