Objective To observe the migration and inhibition mechanism of MicroRNA218-Robo1 pathway for breast cancer.Methods A total of 40 BALB/c-nu/nu female mice were randomly divided into four groups.Each group was transfected over-expression MicroRNA218 MDA-MB-231 breast cancer cells, co-over-expression MicroRNA218 and Robo1 MDA-MB-231 breast cancer cells, knock-down Robo1 MDA-MB-231 breast cancer cells and the control MDA-MB-231 breast cancer cells.The tumor volume was examined every two weeks.Results Tumor volume of MicroRNA218 group was obviously less than control group, tumor volume of Robo1 knock out group was obviously less than common MicroRNA218 high expression and Robo1 group, the difference was statistically significant;MicroRNA218 and Robol knockout group than the control group, the increase in breast cancer cells apoptosis, cell proliferation and angiogenesis is restrained.Conclusions MicroRNA218 inhibited the migration of breast cancer by down-regulating the expression of Robo1.

Objective To analyze the application effect of low-frequency impulse feedback electrical stimulation combined with Buzhong Yiqi decoction and rehabilitation training in promoting postpartum pelvic floor recovery.Methods A total of 90 women with vaginal delivery in our hospital were selected and were given routine pelvic floor rehabilitation training.According to the intervention method,they were divided into the Buzhong Yiqi decoction group,physical therapy group and combined group,with 30 cases in each group.The muscle strength of pelvic floor,muscle contractile function of pelvic floor and complications were compared among the three groups.Results After treatment,the proportion of muscle strength of pelvic floor with grade 4 and grade 5 in the combined group were higher than that in the Buzhong Yiqi decoction group and the physical therapy group (P ＜0.05).After treatment,the duration of pelvic floor muscle contraction and pelvic floor muscle contraction force of the combined group were significantly longer or larger than treatment before and the other two groups (P ＜ 0.05).After 3 days of delivery,the maternal radial artery blood gas of the combined group was higher than that of the physical therapy group and the Buzhong Yiqi decoction group (P ＜ 0.05).There was no significant difference in the incidence of pelvic organs prolapse and urinary incontinence among the three groups (P ＞ 0.05).Conclusion The use of Buzhong Yiqi decoction and low-frequency impulse feedback electrical stimulation combined with rehabilitation training can improve the pelvic floor muscle strength,enhance pelvic floor function and reduce complications.

Objective To analyze the application effect of low-frequency impulse feedback electrical stimulation combined with Buzhong Yiqi decoction and rehabilitation training in promoting postpartum pelvic floor recovery.Methods A total of 90 women with vaginal delivery in our hospital were selected and were given routine pelvic floor rehabilitation training.According to the intervention method,they were divided into the Buzhong Yiqi decoction group,physical therapy group and combined group,with 30 cases in each group.The muscle strength of pelvic floor,muscle contractile function of pelvic floor and complications were compared among the three groups.Results After treatment,the proportion of muscle strength of pelvic floor with grade 4 and grade 5 in the combined group were higher than that in the Buzhong Yiqi decoction group and the physical therapy group (P ＜0.05).After treatment,the duration of pelvic floor muscle contraction and pelvic floor muscle contraction force of the combined group were significantly longer or larger than treatment before and the other two groups (P ＜ 0.05).After 3 days of delivery,the maternal radial artery blood gas of the combined group was higher than that of the physical therapy group and the Buzhong Yiqi decoction group (P ＜ 0.05).There was no significant difference in the incidence of pelvic organs prolapse and urinary incontinence among the three groups (P ＞ 0.05).Conclusion The use of Buzhong Yiqi decoction and low-frequency impulse feedback electrical stimulation combined with rehabilitation training can improve the pelvic floor muscle strength,enhance pelvic floor function and reduce complications.