Objective To explore the regulatory effect of miRNA on B-cell activating factor (BAFF) in chronic eosinophilic rhinosinusitis with nasal polyp (Eso CRSwNP).Methods Ten nasal mucosal samples were collected from Eso CRSwNP patients who were admitted and treated in our hospital between January 2012 and February 2013.Normal nasal mucosal tissues (n =10) served as control.The miRNA-targeting BAFF was predicted by bioinformatics tools.Immunohistochemistry and real time polymerase chain reaction (RT-PCR) were used to assess the protein expression of BAFF and the predicted miR-NA.The correlation between the predicted miRNA and BAFF was analyzed.Results Down-regulated expression of let-a was confirmed in Eos CRSwNP,while the BAFF protein expression was increased.Let-a was positively correlated with BAFF in nasal epithelia.Conclusions Let-a might contribute to mucosal eosinophilia in eosinophilic CRSwNP via targeting BAFF.

To observe the changes of tongue and pulse parameters in lung cancer patients after combined treatment with integrated traditional Chinese and Western medicine, and to probe into the application of the tongue and pulse parameters in evaluation of integrated traditional Chinese and Western medicine therapy on lung cancer patients.

Objective To compare the applied signification of optical and ultrasonic biometry in observing the axial length of eyes with vitreous hemorrhage and retinal detachment. Methods This is a prospective clinical study. We randomly selected 57 cases (57 eyes) with vitreous hemorrhage and (or) retinal detachment from May 1, 2016 to May 31. Patients had not been performed eye surgery, also denied the history of ocular trauma and excluded other eye diseases. 25 eyes with vitreous hemorrhage, 24 eyes with retinal detachment, 8 eyes complicated with vitreous and retinal detachment hemorrhage. At 24 hours before surgery, we measured axial length using the Lenstar LS 900, immersion B-ultrasound (IB) and immersion A-ultrasound (IA). The differences between three methods were assessed with paired sample t-test. Results IB and IA data was collected from all 57 eyes, there was no statistical significance between measurements of IB and IA (P > 0.05). In the eyes with serious refractive medium opacity (36 eyes), the results showed no statistical significance difference between measurements of IB and IA (P > 0.05). The measurements of three groups can be obtained from the rest eyes (21 eyes): significant statistical difference of the measurements between IB and Lenstar was detected[(24.057±1.441)mm vs(23.470±1.775)mm],(t=3.099, P < 0.05) same results between IA and Lenstar was obtained [(23.962±1.443) mm vs (23.470±1.775) mm], difference was statistically significant (t=2.643, P < 0.05). But the measurements showed no statistical significance difference between the measurements of IB and IA (P > 0.05). Conclusions In the eyes with vitreous hemorrhage and (or) retinal detachment, the measurements of axial length could not be achieved or existed deviations on account of refractive medium opacity when using optical biometry. Under such circumstances, we should choose IA or IB as the optimization method to obtain measurements, in order to get much more accurate results.

Objective: To analyse the clinical characteristics of patients of anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV) combined with chronic rhinosinusitis (CRS) and its diagnostic strategy. Methods: A retrospective analysis of 228 patients with AAV treated in Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology from August 2008 to June 2018 was performed. Among them, 17 (7.5%, 17/228) patients(10 males and 7 females, with the range of age from 17 to 67 years old) had CRS and their clinical manifestations, laboratory examinations, treatment and prognosis were summarized and studied. Results: In addition to nasal obstruction and increased nasal secretion, 17 AAV patients with CRS were accompanied by cough, wheezing and other pulmonary symptoms (5/17), fever (7/17) and anemia (7/17). Laboratoy tests showed positive ANCA (14/17), increased C-reactive protein (9/17), haematuria (8/17) and proteinuria (6/17). Except for 2 patients who went to other hospital, most patients (14/15) were relieved by glucocorticoid and immunosuppressive therapy. Conclusions AAV patients with CRS may be accompanied by systemic and pulmonary symptoms (cough, asthma, fever, anemia, etc) or some positive laboratory tests (elevated C-reactive protein, proteinuria and/or hematuria, etc.). To achieve the goal of early diagnosis of AAV, ANCA test and biopsy are recommended for those patients with AAV and CRS concurrently.