Objective To investigate the dynamic change of procalcitonin (PCT ) and hypersensitive C‐reactive protein (hs‐CRP) in neonatal infection and its clinical significance .Methods 176 neonates with infection in our hospital from August 2013 to July 2014 were selected and treated by the routine therapy of anti‐infection ,oxygen inhalation ,etc .,and divided into the improvement group(89 cases) ,non‐change group(48 cases) and deterioration group(39 cases) according to the curative effect after 20 d therapy . Serum PCT and hs‐CRP levels were detected before treatment(T0) ,on 5 d(T1) ,10 d(T2) ,15 d(T3) and 20 d(T4) of treatment by adopting the enzyme linked immunosorbent assay(ELISA) .Results The PCT ,hs‐CRP levels and WBC count before treatment had no significant difference among 3 groups(P>0 .05);the PCT and hs‐CRP levels after treatment in the improvement group showed the significantly declining trend ,which reached the lowest at T4 (P<0 .05) ,while the PCT and hs‐CRP levels in the deterioration group showed the obviously rising trend ,which reached the highest at T4 (P<0 .05) ,but which in the non‐change group had no significant change (P>0 .05) .In terms of WBC count ,the WBC count in all the cases showed a rising first and then decreasing trend ,the WBC count at T4 in the improvement group was lower than before treatment(P<0 .05) ,while which in the deterioration group was higher than before treatment (P<0 .05) ,but which in the non‐change group had no significant change between before and after treatment(P>0 .05);the Spearman analysis results after treatment showed that the serum PCT ,hs‐CRP levels and WBC count were negatively correlated with efficacy (r1 = -0 .764 ,P1 <0 .05;r2 = -0 .724 ,P2 <0 .05 ;r3 = -0 .573 ,P3 <0 .05) .Conclu‐sion PCT and hs‐CRP can effectively reflect the therapeutic effect of neonatal infection ,and is closely correlated with the patient′s own immune status ,which can be taken as one of reference indicators for formulating the treatment scheme and evaluating progno‐sis ,and is worth for further clinical promotion .

Objective:To discuss the clinical significance of procalcitonin(PCT) and hypersensitive c-reactive protein(hs-CRP) test in early newborn infection. Methods:Sixty cases of our hospital treatment confirmed infection early newborn children were selected from June 2007 to July 2007,according to the condition was divided into severe infection group and local infection group, at the same time, 30 cases of our new pediatric infection in early neonatal were chosen as control group, to collect the moderate amount of peripheral blood of three groups before and after the treatment, detected and analyzed the levels of PCT and hs-CRP in the serum, Used Pearson correlation analysis to analysis the correlation of PCT and hs-CRP in the serum. Results: Severe infection group serum PCT, hs CRP levels and positive rate higher than that of local infection group, local infection group of PCT, hs CRP levels and positive rate is higher than the control group, the difference was statistically significant; Infection group before treated with serum PCT and hs-CRP levels and positive rate were higher than after treated(x2=7.248, x2=6.934; P<0.05); Pearson correlation analysis show that, according to the results of PCT and hs-CRP were positively correlated (r=0.533, P<0.533). Conclusion:Procalcitonin and hypersensitive c-reactive protein plays an important role in the infections of early neonatal children, PCT and hs-CRP were positively correlated, it can be used as an important index of early diagnosis of early neonatal infectious diseases.

Objective To analyze the differentially expressed genes in peripheral blood of human immunodeficiency(HIV)/tubercle bacilli co-infected patients and explore the biological regulatory mechanism and network of key proteins,so as to provide new evidence for early diagnosis and clinical treatment of HIV/TB co-infected patients. Methods Microarray gene chip data of HIV/TB co-infected patients were downloaded from public databases GEO and imported into the analysis software GEO,STRING,PANTHER,and GenClip. The gene expression profiles,protein interaction networks,processes of molecular biology,and gene functions were analyzed. Results The expression profiles of 15 529 genes between the two groups of patients were similar,and gene expression profiles from 44 subjects were highly correlated. The 251 differentially expressed genes had good diagnostic capabilities in the differential diagnosis of HIV/TB infection. RPLP1 might be a key gene in the diagnosis of HIV/TB infection. The differentially expressed genes and positive regulators showed certain functions such as external stimuli,signal transduction pathways in cells,migration of neutrophils,and immunological and other relevant functionalities. Meanwhile,they may also be involved in free radical-related apoptosis,inflammation,and activation pathways. Conclusions A total of 251 differentially expressed genes are found to be able to distinguish simple HIV infection from HIV/TB infection. Protein-protein interaction network of top 40 differential expression genes includes RPLP1 gene,which is possibly associated with HIV/TB co-infection and may be involved in and the positive regulation of external stimuli,signal transduction pathways in cells,migration of neutrophils,and immunological functions. These findings may provide certain evidence for the diagnosis and treatment of HIV/TB infection.

AIM: The purpose of this study was to investigate the role of interleukin-4 (IL-4) in the development of periodontitis in mice by low dosage of Trichinella spiralis (T. spiralis) infection.METHODS: Twenty-seven male Kunming mice in specific pathogen free grade were randomly divided into three groups: (1) the normal control group;(2) the experimental periodontitis group, which was produced by ligature of braided silk around the first maxillary molar, and was inoculated with putative periodontopathic bacteria;(3) the periodontitis with T. spiralis infection group. The mice were sacrificed at the end of 1, 3 and 5 weeks. The probing depth (PD) was measured before the mice were euthanized. The histological change of periodontal tissues was observed under the microscope after the samples were stained with hematoxylin and eosin. Furthermore, the serum level of IL-4 was determined by ELISA. RESULTS: (1) The serum level of IL-4 in T. spiralis-infected group was significantly higher than that in experimental periodontitis group (P<0.01). (2) The PD in T. spiralis-infected group was significantly lower than that in experimental periodontitis group (P<0.01). (3) Only a mild inflammatory response was observed in T. spiralis-infected animals. CONCLUSION: T. spiralis infection upregulates IL-4 expression and attenuates periodontitis in mice.

The aim of the study was to investigate the sesquiterpene constituents from the rhizomes of Curcuma wenyujin Y．H．Chen et C．Ling．The isolation and purification of the constituents from the 50％ EtOH extracts of the rhizomes were performed with repeated column chromatography over sillica gel and macroporous resin．Eight sesquiterpenes were obtained and identified as wenyujinlactone A(1),neolitamone A(2),zedoarondiol(3),isozedoarondiol(4),aerugidiol(5),curcumol (6),curdione(7) and (1R,10R)-epoxy-(-)-1,10-dihydrocurdine (8) by means of spectral analysis．Among them,compound 1 was found to be a new eudesmane sesquiterpene lactone,whilst compounds 2-5 were obtained from this plant for the first time．

Objective To evaluate the multi-slice spiral CT (MSCT) features of crush pulmonary parenchymal injuries in the massive Wenchuan earthquake. Methods One hundred and seventeen patients with crush pulmonary parenchymal injuries in the massive Wenchuan earthquake were reviewed, the location, extent and MSCT appearances of the injuries and associated thoracic injuries were evaluated. The MSCT appearances of the injuries on 1 d, 2--5 d and 6--14 d were compared using Chi-square test. Results One hundred and thirteen patients had pulmonary contusion and bilaterally in 70 cases, 7 had pulmonary laceration. A total of two hundreds and eighty-three lung lobes were involved and 3 or more lobes involved in 47 patients. Ill-defined patchy opacity was detected in 75 patients, associated ground-glass opacity (GGO) in 24 cases. Lung laceration presented as pneumatocele in 4 cases and demarcated hematoma in 3 cases. Coexisted rib fractures were detected in 98 patients and flail chests were in 29 patients. Forty-three patients had coexisted fractures of scapula, clavicle and (or) sternum. Ninety-nine patients had coexisted pleural injuries. Conclusions Crush pulmonary parenchymal injuries in the Wenchuan earthquake are severe. The bilateral, multifocal and multiple lobes involved injuries with a high incidence of coexisted other severe thoracic injuries are the noticeable features.

Purpose To observe the mutation of K-ras gene and expression of Fascin-1 protein in CRC tissues and their relationship with clinical pathological features, and then to analyze the correlation between mutation of K-ras and expression of Fascin-1. Methods In 86 cases of CRC tissues, K-ras mutation was detected by DNA sequencing analysis, and Fascin-1 expression was detected by im-munohistochemical method. Results In CRC tissues the mutation rate of K-ras was 34. 88%, the expression rate of Fascin-1 was 60. 47%. The mutation rate of K-ras in lymph node metastasis group was higher than that of without lymph node metastasis group, and that in distant metastasis group was higher than that of without distant metastasis group(P<0. 05). The expression rate of Fascin-1 in serosa invasion group was higher than that of without serosa invasion group, and that in lymph node metastasis group was higher than that of without lymph node metastasis group, and that in distant metastasis group was higher than that of without distant metastasis group (P<0. 01). There was a correlation between the mutation of K-ras gene and the expression of Fascin-1 in CRC tissues (rp =0. 236, P<0. 05). Conclusions The CRC tissues with mutation of K-ras are more likely to metastasize and the CRC tissues with expression of Fascin-1 are more likely to invade serosa and metastasize. The CRC tissues with mutation of K-ras are more likely to express Fascin-1.

Objective To evaluate the effectiveness and safety of tanreqing injection to children bronchial pneumonia.Methods PubMed,Ovid,CNKI,and data retrieval system of all places were searched to identify relevant studies from 2003 to November of 2014.The final documents were examined with inclusive and exclusive criteria.Results were pooled with meta-analysis on RevMan software to assess the efficacy of tanreqing injection to children bronchial pneumonia.Results The database was derived from 13 qualified studies that included a total of 1479 patients.Meta analysis showed that,compared to the control group,tanreqing injection treatment group resulted in higher effective rate [OR =2.96,95％ CI (2.22,3.96),P ＜0.01],shorter time of recovering the normal temperature [MD----0.53,95％ CI (-0.77,-0.29),P ＜ 0.01],shorter time of disappearance of cough [MD =-1.25,95％ CI (-2.14,-0.37),P ＜0.01],shorter time of lungrale [MD=-1.00,95％ CI(-1.4,-0.6),P ＜0.01],and shorter time of hospitalization time [MD =-1.35,95％ CI (-1.83,-0.86),P ＜ 0.01].Significant difference was observed between control and treatment groups.Conclusions This study showed that tanreqing injection appeared to be more effective for children bronchial pneumonia.However,methodology showed low quality.The efficacy needs high-quality clinical trials to verify.

Abstract Survival analysis has been widely used in the field of medical research. The Cox proportional hazard model is commonly used, but its practical application is limited. Machine learning method can compensate for the shortcomings of the Cox proportional hazard model in terms of nonlinear data processing and prediction accuracy. This article reviewed the advance of machine learning methods represented by neural networks, within the field of survival analysis, and highlighted the principles and benefits of three machine learning methods that DeepSurv, Deep-Hit and random survival forest, providing methodological insights for the analysis of complex survival data.

Objective: To investigate the effect of polarizing the immune response toward the Th2 type, using intestinal nematode (Trichinella spiralis) infection, on subsequent experimental periodontitis. Methods:Thirty six SPF KM mice were randomly divided into three groups. Periodontitis group: experimental periodontitis was induced by wrapping a 5/0 silk ligature inoculated with putative periodontopathic bacteria around the first maxillary molar. Th2-polaried group: After the mice were infected with low dosage Trichinella spiralis, the experimental periodontitis model was established at that time as above. Mice were sacrificed at the end of 1, 4, 8, 12 weeks. The histological analysis of periodontal tissues was observed by microscope after the samples were stained with hematoxylin and eosin (H&E). The concentrations of IFN--y and IL-4 in mouse serum were determined by ELISA. Results; The results showed that compared with control group Th2-polaried mice had high levels of IL-4 prior nematode infection reduced the severity of periodontitis, and subsequent infection of the periodontium with oral pathogens developed minimal lesions. Few or no osteoclasts were detected in lesions of Th2-polaried group. Conclusion;These results indicate a protective role of nematode infection in Thl cell-driven periodontal damage and prompt consideration of a novel therapeutic strategy in periodontitis based on im-munological distraction.