1.Quality analysis of the primary semen samples from 512 donors.
Meiyi LIU ; Chunjie MA ; Lixin TANG ; Renqian WEN ; Shunmei DENG ; Qiling WANG ; Yanjia JIANG ; Aiping CHEN
National Journal of Andrology 2004;10(10):734-736
OBJECTIVETo evaluate the semen quality of the donors.
METHODSThe semen parameters of the primary samples of 512 donors were examined following the World Health Organization (WHO) guide and computer-aided sperm analysis (CASA, product by Harmilton Thorne) system.
RESULTSOf the 246 (246/512, 48%) donors with potential fertility, only 146 (146/512, 28.5%) came up to the semen standard set by the Chinese Ministry of Health, while 266 (266/512, 52%) were below the WHO reference values of semen parameters.
CONCLUSIONRepeat semen analyses may increase the success rate in screening semen donors. The semen quality of the donors suggests that it is necessary to pay more attention to male reproductive health.
Adult ; Humans ; Image Processing, Computer-Assisted ; Male ; Mass Screening ; Middle Aged ; Reference Values ; Semen ; physiology ; Sperm Banks ; Sperm Count ; Sperm Motility ; Tissue Donors
2.Comparison of sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis.
Meiyi LIU ; Shunmei DENG ; Chunjie MA ; Aiping CHEN ; Yanjia JIANG ; Renqian WEN ; Qiling WANG ; Lixin TANG ; Jianchu HUANG ; Xiaotao YAO
National Journal of Andrology 2004;10(6):431-433
OBJECTIVETo observe sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis (CASA) system.
METHODSSemen analyses of 238 samples before freezing and after thawing were separately performed by Hamilton-Thorne Sperm Analyzer.
RESULTSSperm motility in post-thaw samples was significantly decreased. There was significant correlation and difference between pre-freeze and post-thaw samples in sperm motion parameters, including average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), straightness (STR) and linearity (LIN), except heat cross frequency (BCF). The percentage of sperm movement velocity parameters (VAP, VSL and VCL) and moving pattern parameters (ALH) significantly decreased, while that of LIN and STR significantly increased in post-thaw samples.
CONCLUSIONCASA system is of clinically applied value and is a useful tool for evaluating sperm motion parameters in pre-freeze and post-thaw semen samples.
Cryopreservation ; Diagnosis, Computer-Assisted ; Freezing ; Humans ; Male ; Sperm Motility
3.Total flavonoids of Pterocarya hupehensis Skan inhibit migration and in-vasion of fibroblast-like synoviocytes by regulating PI3K/AKT signaling pathway
Hao WU ; Guoqing CHEN ; Man LU ; Ying GAO ; Qiling JIANG ; Haonan LUO ; Ling YUAN ; Yang XIANG
Chinese Journal of Pathophysiology 2024;40(1):134-140
AIM:To observe how total flavonoids of Pterocarya hupehensis Skan(PHSTF)affects the migra-tion and invasion of human rheumatoid fibroblast-like synoviocyte line MH7A.METHODS:The MH7A cells were divided into control group(without any treatment),low-,medium-and high-dose(6.25,12.5 and 25 mg/L,respectively)PHSTF groups,phosphatidylinositol 3-kinase(PI3K)inhibitor 740Y-P(10 μmol/L)group,and 740Y-P(10 μmol/L)+high-dose(25 mg/L)PHSTF group.The viability of the MH7A cells was determined by CCK-8 assay,while the migration and inva-sion were assessed by scratch and Transwell assays.The protein levels of matrix metalloproteinase-2(MMP-2),MMP-9,PI3K,p-PI3K,AKT and p-AKT were detected by Western blot.RESULTS:Compared with control group,the cell via-bility in PHSTF treatment groups was reduced(P<0.05),the cell wound healing area was significantly decreased(P<0.01),migratory and invasive cells in the lower chamber were significantly reduced(P<0.01),and the protein expres-sion of MMP-2 and MMP-9 and the ratios of p-PI3K/PI3K and pAKT/AKT were decreased(P<0.01).Compared with high-dose PHSTF group,the addition of PI3K/AKT pathway agonist 740Y-P significantly increased the migration and invasion ability of MH7A cells(P<0.01)and elevated the protein expression of MMP-2 and MMP-9 and the ratios of p-PI3K/PI3K and pAKT/AKT(P<0.01)under the treatment with PHSTF.CONCLUSION:Total flavonoids of Pterocarya hupehensis Skan could inhibit the migration and invasion of MH7A cells by regulating the PI3K/AKT signaling pathway.
4.Non-invasive brain stimulation for Parkinson's disease in decade: a bibliometrics analysis
Qiling XU ; Xiaoyu JIANG ; Hongyan BI
Chinese Journal of Rehabilitation Theory and Practice 2024;30(6):665-674
ObjectiveTo analyze the current status, hotspots and trends of researches related to non-invasive brain stimulation for Parkinson's disease in the last decade. MethodsLiterature related to non-invasive brain stimulation for Parkinson's disease in the Web of Science Core Collection was searched from January, 2014, to December, 2023, and CiteSpace 6.1.R3 was used to draw a knowledge map of authors, institutions, countries and keywords. ResultsA total of 1 351 papers were included, with a general upward trend in the number of publication, with the United States, Italy and China being the high-producing countries, and the University of London being the most prolific institution, with Alfredo Berardelli being the most prolific author. Hot keywords included deep brain stimulation, motor cortex and basal ganglia, and emergent words that have appeared in the last two years included therapy, non-invasive brain stimulation, supplementary motor areas and movement disorders. ConclusionThe research hotspots related to non-invasive brain stimulation for Parkinson's disease in the last decade mainly focus on the stimulation on motor cortical areas, the combined application of non-invasive brain stimulation and deep brain stimulation, and non-invasive brain stimulation and non-invasive neuroimaging techniques, such as electroencephalography and functional imaging. Non-invasive brain stimulation of supplementary motor areas can be further explored to improve movement disorders in Parkinson's disease patients in the future.