Objective:To investigate the physical and mental health of trainees receiving standardized residency training of anesthesiology in four large teaching hospitals in Wuhan, China, as well as related risk factors.Methods:The trainees who received standardized residency training of anesthesiology in four large general teaching hospitals in Wuhan were selected as subjects, and Symptom Checklist-90 (SCL-90) and Eysenck Personality Questionnaire were used to investigate mental health and personality state. SPSS 22.0 was used to perform the independent samples t-test, a one-way analysis of variance, and the chi-square test, and Logistic regression was used to analyze the influencing factors for SCL-90. Results:A total of 328 questionnaires were distributed and 326 valid questionnaires were collected, with a recovery rate of 99.39%. Among these trainees, 68 (20.86%) were found to have abnormal mental status (SCL-90 score >160), with a relatively high proportion of the trainees with obsessive-compulsive symptoms or depression. The trainees with abnormal mental status showed the personality characteristics of tendency neuroticism and tendency psychoticism, and compared with the trainees with normal mental status, the trainees with abnormal mental status showed a higher proportion of trainees with abnormal personality characteristics. The univariate analysis showed that the grade of residency training, scientific research pressure, monthly on-duty frequency, and personality characteristics were associated with abnormal mental status, and the multivariate analysis showed that a monthly on-duty frequency of >5 times ( B=0.997, odds ratio [ OR]=2.709, 95%CI: 1.051-6.986, P=0.039), great pressure of scientific research ( B=3.235, OR=25.412, 95%CI: 3.239-199.363, P=0.002), emotional instability ( B=2.015, OR=7.504, 95%CI: 3.529-15.954, P<0.001), and psychosomatic abnormalities ( B=1.640, OR=5.154, 95%CI: 2.510-10.581, P<0.001) were risk factors for psychological abnormality in trainees, while extroversion personality ( B=-2.758, OR=0.063, 95%CI: 0.019-0.215, P<0.001) was a protective factor against abnormal mental status. Conclusions:The positive rate of psychological abnormality is 20.86% among trainees receiving standardized residency training of anesthesiology in four large teaching hospitals in Wuhan, and possible influencing factors include monthly on-duty frequency, scientific research pressure, work pressure, and different personality characteristics.

Objective To investigate how suicide microblog users in China "act" and "speak" differently from other microblog users without suicide idea.Methods The suicide group consisted of 31 Chinese microblog users identified as suicide via online information provided by a Sina microblog user,and the control group consisted of 30 active microblog users without suicide idea screened by using suicide-related psychological scales.The differences in 10 microblog use behavioral characteristics and 88 linguistic characteristics between the suicide group and the control group were compared with normality test and rank sum test respectively.Results In the behavioral characteristics,the suicide group used hyperlinks and "@" less frequently than the control group [0.04 (0.04) vs.0.06 (0.04),P=0.029;0.60 (0.27) vs.0.69 (0.18),P=0.028],and was more self-focused [0.47 (0.25) vs.0.30 (0.10),P=0.010].In the linguistic characteristics,the suicide group showed less frequency in using measure word,work related word and apostrophe than the control group (P＜0.05),and showed more frequency in using pronoun,personal pronoun,third person singular,non-specific pronoun,word expressing social experience,word expressing anxiety,word expressing exclusion,sexual word,religious word,second person singular,human being related word,negative emotion related word,anger related word,sadness or death related word (P＜0.05).Conclusion Suicides seemed to interact less with others,showed more self-concern and more negative expressions,use more cognitively exclusive,death-related,religion-related words,and use less work-related words.The results of this study might be helpful for the research on suicide among netizen.

Objective:To investigate the effects of hydroxysafflor yellow A (HSYA) on depressive-like behavior and expression of type A γ-aminobutyric acid receptor(GABAAR)in hippocampus of chronic restraint stress model mice.Methods:The SPF grade male C57BL/6C mice were divided into Control group, HSYA group, Model group, Model + HSYA group and Model + fluoxetine group according to random number table method, with 12 mice in each group.Mice model of depression was established by chronic restraint stress.Mice in HSYA group and Model+ HSYA group were intraperitoneally injected with HSYA(20 mg/kg), mice in Model+ fluoxetine group were injected intraperitoneally with fluoxetine (10 mg/kg), and mice in Control group and Model group administered with 0.9% sodium chloride solution intraperitoneally once a day for 14 days.Then, the forced swimming test (FST) and tail suspension test (TST) were performed to evaluate the depressive-like behavior of mice, and the protein expression levels of different subtypes of GABAAR in the hippocampus of mice were determined by Western blot.SPSS 19.0 and GraphPad Prism 8.0 software were used for data statistical analysis and mapping.One-way ANOVA was used for comparison among groups, and Tukey-HSD test was used for further pairwise comparison.Results:(1) In the behavioral tests, there were significant differences in swimming immobility time of FST and tail suspension immobility time of TST among the five groups ( F=21.59, 20.81, both P<0.05). The swimming immobility time ((143.91±9.97) s) and tail suspension immobility time (( 107.00±6.54) s) in Model group were higher than those in Control group ((52.92±6.70) s, ( 43.50±5.96) s, both P<0.05). There were no significant difference in swimming immobility time and tail suspension immobility time between Model+ HSYA group ((26.17±7.69)s, ( 20.17±7.89)s) and Model+ fluoxetine group ((61.60±16.22)s, (34.14±10.74)s)(both P>0.05), but the swimming immobility time and tail suspension immobility time in these two groups were lower than those in Model group (both P<0.05). (2) The Western blot results showed that there were significant differences in the expression of GABAARβ1 and GABAARβ2 protein in hippocampus among the four groups ( F=12.21, 11.40, both P<0.05). The expression levels of GABAARβ1(45.60±10.76) and GABAARβ2 (46.27±4.82) protein in hippocampus of Model group were lower than those in Control group ((100.00±3.44), (100.00±3.26), both P<0.05). Compared to Model group, the expression of GABAARβ1 (79.91±5.00) and GABAARβ2 (79.08±5.53) protein in hippocampus of Model+ HSYA group were higher (both P<0.05). In addition, the expression of GABAARα1 and GABAARγ1 proteins in hippocampus were not significantly different among the four groups( F=0.23, 0.10, both P>0.05). Conclusion:HSYA can effectively alleviate depressive-like behavior in depression model mice, which may be related with the upregulation of GABAARβ1 and GABAARβ2 of hippocampus tissue.