Objective To investigate the incidence of adverse drug reaction (ADR)in patients in a hospital,and evaluate ADR-related factors,so as to provide references for the prevention of ADR and reducing of risk factors in drug use. Methods 223 clinically reported ADR cases were collected from January 1,2011 to December 31,2011, patients'age,types and administration routes of drugs relating to ADR,and ADR-involved organs and systems were analyzed.Results Of 223 patients with ADR,211(94.62% )received intravenous drip,3 received intravenous injec-tion,2 received intramuscular inj ection,and 7 received oral administration. ADR mainly occurred within 1-30 mi-nutes after taking the medicines,39(17.49% )occurred within 1-4 minutes,45(20.18% )within 5-9 minutes,80 (35.87% )within 15-29 minutes. Of organ or system involved by ADR,skin and its appendage damages were com-mon (93 cases),followed by gastrointestinal tract (62 cases)and systemic damage (45 cases);single organ and sys-temic damage were most common (184 cases,82.51% ). 123 cases (55.16% )of ADR were caused by antimicrobial use,60 cases (26.91% )were induced by Chinese medicine injection;Of top 10 ADR-inducing medicines,7 were an-timicrobial agents and 3 were traditional Chinese medicine injections,levofloxacin lactate and sodium chloride injec-tion ranked first(23 cases,10.31% ).Conclusion The occurrence of ADR is related to multiple factors,reporting and surveillance of ADR should be strengthened,the use of antimicrobial agents and traditional Chinese medicine should be rational,thereby reduce the occurrence of ADR.

Objective To explore the protective effect and mechanism of vitamin D combined with puerarin on liver fibrosis.Methods The rats were divided into normal control group (C),tetrachloromethone group (CCl4),vitamin D group (V),puerarin group(P) and vitamin D combined with puerarin group(V+P).After 8 weeks,the rats were sacrificed and blood and liver samples were collected.The level of blood hyaluronic acid(HA) was tested.The hydroxyproline(Hyp) level in the liver was measured.The liver paraffin sections were made and examined by the sirius red staining.The mRNA levels of collagen Ⅰ and collagen Ⅲ in the liver tissue were detected by RT-PCR,and the levels of NF-κB and TNF-α in the liver were detected by Western blot.Results The CCl4 group appeared obvious liver fibrosis.The liver fibrosis degree was significantly improved in the group V,P and V+P,the blood HA level and liver Hyp level were reduced.The mRNA levels of collagen Ⅰ and collagen Ⅲ as well as the protein levels of NF-κB and TNF-α in the liver were significantly decreased.Among them.The liver fibrosis improvement degree in the V+P group was most significant.Conclusion Vitamin D combined with puerarin can protect rat liver fibrosis induced by CCl4 and its mechanism may be related with reducing the activation of hepatic stellate cells(HSC) and decreasing the collagenous fibers secretion.

OBJECTIVETo map the gene responsible for nonsyndromic hearing impairment in a consanguineous family.

METHODSFirstly, X chromosome scanning was used to exclude X chromosome. Secondly, candidate gene analyzing and genome scanning were performed by homozygosity mapping. Then, additional markers flanking the tightly linked marker were tested to confirm linkage and decide the candidate region.

RESULTSThe nonsyndromic hearing impairment of this family was autosomal recessive. Twenty-five known genes were excluded. Autosomal genome scanning indicated that D17S1293 was tightly linked with disease gene. And further study mapped the disease gene to a 5.07 cM interval bounded by D17S1850 and D17S1818.

CONCLUSIONThe disease gene of the family is mapped to a 5.07 cM interval between D17S1850 and D17S1818, which is a new locus of autosomal recessive nonsyndromic hearing impairment.

Chromosome Mapping ; methods ; Chromosomes, Human, Pair 17 ; genetics ; Chromosomes, Human, Pair 18 ; genetics ; Chromosomes, Human, X ; genetics ; Consanguinity ; Family Health ; Female ; Genetic Predisposition to Disease ; genetics ; Hearing Loss, Sensorineural ; genetics ; Humans ; Male ; Microsatellite Repeats ; Pedigree

OBJECTIVETo study the prevalence of methylenetetrahydrofolate reductase (MTHFR) C677T genotype and its association with deep vei n thrombophilia in Chinese.

METHODSPolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was conducted to examine mutation with 63 deep vein thrombophilic patients and 80 health controls in Shandong Hans. The genotype frequencies were calculated by gene counting in patients and controls, and an analysis was made on the association of MTHFR C677T mutation with deep venous thrombosis in Shandong Hans.

RESULTSIn case- controls, the frequencies of C/T heterozygote were 41.27% and 43.75%; whereas those of T/T homozygote were 52.38% and 36.25%. Significantly elevated mutation was observed in patients(Chi-square=6.372, P 0.01 OR(T/T)=4.552 95% confidence interval:1.440-14.390, Chi-square =6.742 P=0.009).

CONCLUSIONThe C677T mutation of methylenetetrahydrofolate reductase gene is a risk factor associated with deep vein thrombophilia in Shandong Hans.

China ; DNA ; genetics ; Gene Frequency ; Genotype ; Humans ; Methylenetetrahydrofolate Reductase (NADPH2) ; Odds Ratio ; Oxidoreductases Acting on CH-NH Group Donors ; genetics ; Point Mutation ; Polymorphism, Restriction Fragment Length ; Thrombophilia ; enzymology ; genetics ; Venous Thrombosis ; enzymology ; genetics