Objective To observe the acute toxic reaction and death of mice one day after intragastric administration of Baicalein capsule so as to appraise its safety.Methods The ICR mice were intragastrically administered with Baicalein powder at maximum concentration. There was no death of mice found and no LD50 detected. Hence the maximum dosage was measured. Results The maximum dosage of Baicalein powder is 15g/kg. Conclusions The Baicalein capsule is relatively safe.

Objective To observe the acute toxic reaction and death of mice one day after intragastric administration of Baicalin capsule so as to appraise its safety. Methods The ICR mice were intragastrically administered with Baicalin powder in capsule at maximum concentration. There was no death of mice found and no LD_ 50 detected after administration. Hence the maximum dosage was identified. Results The maximum dosage of Baicalin powder is 15g/kg. Conclusions The Baicalin capsule is relatively safe.

Objective To observe the effect of emodin capsule on constipation model mice caused by diphenoxylate and compare it with the effect of Rhubarb & sodium bicarbonate tablet.Methods The ICR mice were divided into 6 groups as blank control group, diphenoxylate model group, emodin 25mg/kg,50mg/kg,100mg/kg group, and Rhubarb & sodium bicarbonate tablet 500mg/kg group. The blank control group and model group were given distilled water intragastrically while the rest 4 groups were given certain doses. The time of first dark stool defecation and 6-hour defecation quantity of each mouse were observed and recorded. The total weight of stool was weighed.Results The time of first dark stool defecation of the model group is markedly longer than that of control group (P

Objective To explore the regulatory effect of astragalus polysaccharide ( APS) on immune function in immunosuppressed mice, and to establish a set of feasible indexes of immunological regulation and evaluation system of pol-ysaccharides.Methods Both Cytoxan ( CTX)-induced immunosuppressed mice and normal mice were treated with astrag-alus polysaccharide ( APS) .Spleen index, thymus index, phagocytosis rate and phagocytic index of peritoneal macrophages were assessed, and the spleen and thymus tissues were examined by histology.The percentage of CD3 +, CD4 +, and CD8 +cells in peripheral blood were determined by flow cytometry.Results APS significantly increased the thymus and spleen indexes in both immunosuppressed mice and normal mice, promoted the histological development of spleen and thy-mus in normal mice, enhanced the recovery of histological structures in the spleen and thymus in the CTX-induced immuno-suppressed mice.APS also enhanced the phagocytosis rate and phagocytic index of peritoneal macrophages in both immuno-suppressed mice and normal mice.In addition, the percentage of CD3 +and CD4 +cells,the ratio of CD4 +/CD8 +in pe-ripheral blood of both CTX-induced immunosuppressed mice and normal mice were increased while CD8 +cells were de-creased.Conclusions The results of this study suggest that astragalus polysaccharide can improve the non-specific immu-nity and cellular immunity in mice, and indicate that we established comprehensive evaluation indexes of immunoregulatory effects of polysaccharides.

OBJECTIVE:To study the chemical constituents of the flower of Zang Epipremnum aureum. METHODS:The con-stituents of ethyl acetate extract of the flower of E. aureum were separated and purified with varied chromatographic techniques, and the structures were identified based on spectral data and chemical properties. RESULTS:Ten compounds were isolated from eth-yl acetate extract of the flower of E. aureum,they were identified as rutamontine(1),edgeworoside C(2),edgeworin(3),tiliro-side (4),helichrysoside (5),kaempferol (6),2,4-dihydroxypheny-2-hydroxy-4-metho-xybenzyl-ketone (7),ethyl caffeate (8), phthalic acid bis-(2-ethyl-hexyl) ester (9) and noreugenin (10). CONCLUSIONS:Compound 7 is firstly isolated from natural source,compounds 5,8 and 9 is firstly isolated from the family Thymelaeaceae,compound 6 is firstly isolated from Edgeworthia and compound 2 and 10 are firstly isolated from the flowers of E. aureum. The study lays certain foundation for the quality evalua-tion of E. aureum.

ObjectiveTo investigate the value of urinary BNP for diagnosis of chronic heart failure (CHF). MethodsThe levels of Urinary BNP and plasma BNP were measured by microparticle enzyme immunoassay (MEIA) in 83 patients with CHF and 30 control subjects. The heart function was classified according totheNYHAcriteria. Leftventricularejectionfractions(LVEF)weremeasuredby echocardiology. ResultsThe level of urinary BNP in patients with CHF was[90. 0(38. 3 -209. 5 )]ng/L and the level of plasma BNP was[680. 0 ( 289. 7 - 1543.5)]ng/L, both of them were much higher than those in healthy subjects,[17. 0 ( 13.0 - 33. 0)]ng/L and[84. 5 ( 56. 0 - 158.0 )]ng/L, respectively (P＜0. 01 ). The concentrations of urinary BNP increased gradually with more severe symptoms ( NYHA cl ass Ⅰ -ⅣV ). The level of urinary BNP was positively correlated with NYHA class ( r = 0. 742, P ＜ 0. 01 )and the level of plasma BNP (r =0. 842,P ＜0. 01 ) while negatively related with LVEF (r = -0. 801 ,P ＜0. 01 ). The level of urinary BNP in patients with LVEF ＜ 40％ was[143.0 ( 85. 0 - 258.0)]ng/L , which was much higher than that in patients with LVEF≥40％ ,[31.5( 17.3 -38. 8)]ng/L, (P ＜0. 01 ). At a decision threshold of 36. 5 ng/L, the urine BNP assay demonstrated a clinical sensitivity and specificity of 84％ and 80％ ,respectively. In this study,the area under the curve(AUC) was 0. 905. ConclusionUrinary BNP is a new candidate marker for the diagnosis of CHF,it provides a similar accuracy with plasma BNP.

Objective To investigate the effect of doxapram on inhibition of medullary respiratory center excitability by sevoflurane in rats.Methods Neonatal Sprague-Dawley rats of both sexes,aged 1-4 days,were used in this study.Isolated medulla oblongata-spinal cord specimens were made according to the method described by Suzue and perfused with the artificial cerebrospinal fluid saturated with 95％O2-5％CO2.The specimens were randomly divided into 3 groups ( n =9 each):control group (group C),sevoflurane group (group S) and sevoflurane + doxapram group (group S + D).Respiratory rhythmical discharge activity of the hypoglossal nerve rootlets was recorded using suction electrode.After 10 min of equilibration,the specimens were perfused with the artificial cerebrospinal fluid,5％ sevoflurane and the mixture of 5％ sevoflurane and 5 μmol/L doxapram for 10 min in groups C,S,and S + D respectively.The respiratory cycle,inspiratory time and integral amplitude of inspiratory discharge were recorded.Results Compared with group C,the respiratory cycle was significantly prolonged,the inspiratory time was significantly shortened,and the integral amplitude of inspiratory discharge was significantly decreased in group S (P ＜ 0.05),and no significant change was found in the parameters mentioned above in group S + D (P ＞ 0.05).Compared with group S,the respiratory cycle was significantly shortened,the inspiratory time was significantly prolonged,the integral amplitude of inspiratory discharge was significantly increased in group S + D ( P ＜ 0.05).Conclusion Doxapram antagonizes sevoflurane-induced inhibition of excitability of medullary respiratory center in rats.

Objective To study the effects of sulfur mustard on chromosomal aberration in Chinese hamster fibroblast cell line (CHL cells) and the micronucleus (MN) frequency in the bone marrow cells of mice. Methods CHL cells (with and without S9) treated with different concentrations of sulfur mustard for 24 h were harvested for the preparation of chromosome. Subsequently, the frequency and the type of chromosomal aberration were examined. The KM mice were sacrificed at 24 h after subcutaneous injection of sulfur mustard. The number of micronuclei-containing polychromatic erythrocytes (PCEs) was counted by means of bone marrow smear and Giemsa staining. Results Sulfur mustard might increase the chromosomal aberration frequency of CHL cell in a dose-dependent manner. S9 had no effects on the classificaiton and the frequency of chromosomal aberration. The types of chromosomal aberration included gap, break, deletion, polyploid, and so on. Compared with that in the control group, the MN ratio in every group increased significantly (P

Objective:To analyze the experience of XU Peng-lin in using medicine in treating chronic gastritis,exploring the method for summarizing the experience of famous TCM practitioner.Methods:Professor Xu's 212 typical cases of chronic gastritis were collected,using related rules and point mutual information to analyze the prescription compatibility for the chronic gastritis.Results:Utilization of 12 kinds of drugs were over 30 percents,includinging Pinellia,dried ginger,mongolian milkvetch root,Medicinal Evodia Immature Fruit,and liquorice root,etc..The common compatibility rules were warming spleen-stomach to eliminat dampness,acridness and sweetness activating yang,sour-sweet herbs nourishing yin,pungency disperses while bitterness descends,application of both cold and heat,and combination of reinforcing and reducing.The basic prescriptions for chronic gastritis used by professor XU were Pinelliae Decoction for Purging Stomach-Fire,Zuojin Pill,Huangqi Jianzhong Decoction.Conclusion:Harmonizing yin and yang,reinforcing deficiency and reducing the excess,regulating the activities of qi,harmonizing zang and fu organs were the treatment for chronic gastritis by professor XU,which provided the methodological reference toTCM in treating the chronic gastritis.

0.05).CONCLUSIONS Piperacillin/tazobactam in the treatment of urethral stricture complicated with infection is significantly effective and safely.