Objective To observe the acute toxic reaction and death of mice one day after intragastric administration of Baicalein capsule so as to appraise its safety.Methods The ICR mice were intragastrically administered with Baicalein powder at maximum concentration. There was no death of mice found and no LD50 detected. Hence the maximum dosage was measured. Results The maximum dosage of Baicalein powder is 15g/kg. Conclusions The Baicalein capsule is relatively safe.

Objective To observe the acute toxic reaction and death of mice one day after intragastric administration of Baicalin capsule so as to appraise its safety. Methods The ICR mice were intragastrically administered with Baicalin powder in capsule at maximum concentration. There was no death of mice found and no LD_ 50 detected after administration. Hence the maximum dosage was identified. Results The maximum dosage of Baicalin powder is 15g/kg. Conclusions The Baicalin capsule is relatively safe.

Objective To evaluate the value of event-related potential mismatch negativity(ERP-MMN)for diagnosis and prognosis of amnestic mild cognitive impairment(aMCI).Methods 181 people were enrolled in this study,including 102 aMCI patients and 79 normal people and all of them underwent the clinical symptom and performance of neuropsychologieal measures and cranium imaging and examination of MMN.aMCI patients were judged to progress to Alzheimer's disease(AD)or not by clinical symptom and performance of neuropsychological measures during 1 year follow-up period.Through analysis of peak latency (PL)and amplitude(Amp),the value of MMN in diagnosis of aMCI and normal subjects was assessed,judging aMCI progress to AD or not.Results In contrast to normal control subjects(NC),PL of MMN in subjects of aMCl was significantly longer and Amp was significantly shorter,PL of MMN(312.7±25.7)ms vs(285.1±28.8)ms(t=6.81,P<0.01),Amp(3.5 ±1.3)μV vs(4.7±1.1)μV(t=-6.37,P<0.01).Moreover,PL of MMN in subjects of aMCl which would progress to AD was significantly longer than of subjects of aMCI which would not progress,(332.2±24.1)ms vs(307.5 ±24.0)ms(t=2.75,P=0.007).However.significantly difference was not observed in Amp of MMN and neuropsychological measures between subjects of aMCI would progress and not.Conclusions MMN can be used in auxiliary diagnosis and judging prognosis of aMCI.It is appropriate when is required to reflect and monitor periodic cognitive state of subjects of aMCI.

Objective To observe the effect of emodin capsule on constipation model mice caused by diphenoxylate and compare it with the effect of Rhubarb & sodium bicarbonate tablet.Methods The ICR mice were divided into 6 groups as blank control group, diphenoxylate model group, emodin 25mg/kg,50mg/kg,100mg/kg group, and Rhubarb & sodium bicarbonate tablet 500mg/kg group. The blank control group and model group were given distilled water intragastrically while the rest 4 groups were given certain doses. The time of first dark stool defecation and 6-hour defecation quantity of each mouse were observed and recorded. The total weight of stool was weighed.Results The time of first dark stool defecation of the model group is markedly longer than that of control group (P

OBJECTIVE:To optimize extraction technology of flavonoids from Glechoma longituba by aqueous two-phase partitioning with ultrasonic extraction.METHODS:The extraction technology of flavonoids from G.longituba were optimized by orthogonal experiment with ultrasonic extraction time,the volume fraction of propanol,the ratio of solid to solution,amount of(NH4)2SO4 as factors and with extraction rate of flavonoids from G.longituba as index.RESULTS:The optimal conditions were as follows:extracting for 30 min with ultrasonic wave,the volume fraction of propanol 50%,the ratio of solid to solution 1:30,and the amount of(NH4)2SO4 30%.CONCLUSION:Established extraction technology which is characterized with simple operation and mild conditions shows great advantage.

To investigate the serum level changes of tumor related factors in patients with mechanical trauma and with acute inflammation, and to inquire into the possible reason and its clinical significance, TSGF level was detected on day 1, 3 and 12 after trauma or inflammation in 60 patients with mechanical trauma (39 with mild trauma and 21 with severe trauma) and 37 patients with acute inflammation, while 40 healthy persons were selected as the normal control group. TSGF serum level was significantly higher in patients with trauma and with infectious inflammation than that in normal control group on day 1 and day 3( P 0 05).The results suggest that TSGF is related with the recovery of trauma and inflammatory response.

Objective To study the influence of progesterone on the cell proliferation of hormone?dependent breast cancer and observe the co?effect of vitamin D and progesterone of different levels on the proliferation of the cell line T?47D in hormone?dependent breast cancer and the interaction be?tween vitamin D and progesterone. Methods The cultured T?47D cells were divided into the high and the low progesterone mono?treated groups to observe the effects of different levels of progesterone on the cell proliferation of hormone?dependent breast cancer cell line(T?47D). The high and the low vitamin D mono?treated groups were set to observe the effects of different levels of vitamin D on the cell proliferation. Groups treated by differ?ent levels of progesterone combined with vitamin D were set to observe the interaction between them. Normal breast cancer cells were set as the con?trol group. The two?factor two?level parallel factorial experiment was conducted to observe the co?effect of different levels of progesterone and vitamin D on the proliferation of T?47D. The growth and apoptosis of cells was observed through detection of absorbance in each group by MTT. Results The cell concentration in high and low progesterone treated groups was increased than that in the control group,and was increased in the low proges?terone treated group compared with the high progesterone treated group(P<0.05). The cell concentration in dual?low level treated group was de?creased than that in the other three groups which were two?factor treated and the control group(P<0.05). Conclusion Progesterone stimulates the cell proliferation of hormone?dependent breast cancer. The concentration?based interaction between vitamin D and progesterone indicates that the bi?directional effect of progesterone on breast cancer cells may be related to the concentration of progesterone and other factors,but the specific interac?tion and the mechanism is unclear.

Objective To investigate the feature and the safety of birth process of induced labor by Propess.Methods The 380 cases of vaginal delivery women in our hospital between Sep.2006 to Dec.2007 who, were primigravida,term delivery,singleton gestation,vertex presentations and no standing contraindication, was considered as study group. The other 380 cases of women of labor spontaneous or intravenous oxyvenous in the same condition was considered as control group.The birth process,the amount of bleeding,the number of urine retention,and the prognosis of neonate were compared between the two group.Results The active phase and the second stage was abbreviated in the study group,(P0.05).Conclusion Propess was more effective and safe in shortening the active phase, the secona stage and reducing complications.

Objective To evaluate the clinical applied value of serum vascular endothelial growth factor (VEGF) in therapeutic process of patients with malignant tumor. Methods Serum VEGF levels and positive rates were measured by ELISA in 337 untreated cancer patients, 25 postoperative and 22 recurring postoperative patients with malignant tumor, while 47 benign tumor and 61 healthy individuals as controls. Results Serum VEGF levels and positive rates were significantly higher in patients with various malignant tumors than in benign tumors and healthy controls (P 0.05). Conclusions It was helpful to get a message of pathogenetic condition of malignant tumor patients whose serum VEGF was dynamically monitored.

Objective:To study the effects of the fastigial nucleus electrostimulation on the ability of learning and memory and the Apoptosis of the neurons in the hippocampal district in ratymodel of Alzheimer's D isease induced by?-amyloid(A?).Methods:Forty rats were randomly divided into:A?1-40 micro-injection groups(AD),sham-operation roup(SC),A?1-40 micro-injection and fastigial nucleus stimulation group(FNS),a A?1-40 micro-injection and corpus dentatum stimulation group(DNS).The effect of fastigial nucleus electrostimulation on learning and memory retention disorder in the model rats was studied by the test of Morris water maze;the brain protection of fastigial nucleus electrostimulation in model rats was observed through pathomorphology changes;the Apoptosis of the neurons in the hippocampal CA1 district was detected by Terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling method.Results:The escape latency of the place navigation in the AD grou,FNS group and DNS group were singnificiantly longer than that in the SC group(P