When an extremity is being lengthened, the principle of the elasticity limitation of living tissues is always considered to be a guide fo avoid injuries to the nerves, blood vessels and periostium. It is generally believed that the lengthening should not exceed 10~15% of the original length of the bone. In order to attain a safe and large scale lengthening of a limb, the authors, under the condition of secure fixation of bone ends and early functional excercise of the diseased limb by using a specially des-gned external fixator, adopted a gradual lengthening with a slow speed of 1~1.25mm/d.Eighty cases of epiphysial distraction and 63 cases of metaphyseal osteotomy to lengthen extremities were studied. It was found that the expected lengthening in all the cases was achieved with a lengthening rate of 9~84.3% of the original length. It was 30~84.3% in 16 cases. The absolute lengthening was 6~16 cm in 69 cases. In one case, the femur and tibia were elongated at the same time, and a large shortening of 22 cm of the right lower limb was corrected in a one-stage operation (131 days).Experiments were performed on 15 goats. The lengthening rate of the tibia was 28.6~64.8% of the original length, with an average of 50.2%. In both the clinical and experimental studies, no nerve or blood vessel injury or non-union of the bone was found.The authors proposed a new concept different from the principle of elasticity limitation of livingissues. It is believed that nerves, blood vessels and periostium possess good adaptation capacity to slowand gradual lengthening. Low tension from traction can stimulate the tissues of the extremity to grow synchronously with the slow gradual lengthening and no injury will be inflicted to the structures and functions of the tissues concerned.

Objective To evaluate the effect of limb lengthening on the perineurial barrier and vascular permeability of peripheral nerve. Methods Thirty-two rabbits with limb lengthening of 10％ , 20％ , 30％ and 40％ were used in this study; 8 animals were allocated in each group. Sixteen were employed for the electron microscopic(EM) observation and the others for the investigation of perineurial barrier and vascular permeability of the tibial nerves. Results Even if the elongation reached 40％ of the original length of the tibia, both the perineurium and inner membrane of capillary was still intact, the perineurial barrier and vascular permeability maintained normal; however, EM examination revealed increase of a large number of vacuoles and the increase of the number of mitochondria in the cell of the perineurial membrane. Conclusion It is the gradual limb lengthing of

Sixty cases of different types of patellar fractures were treated from December 1986 to October 1990 by a self-designed miniature external fixator. The patients were followed-up for 6 months to 4 years, and there was not a single case of infection, prepatellar bursitis or early degeneration of the knee joint. The structure of the fixator is simple and the operation is easy. It is a versatile tool, because it can be used either for extension or for compression.since the pins are inserted without operation, it gives rise to little pain. Under the present treatment bone healing is rapid and the function of the knee joint is well preserved. The rate of excellent result reaches 98.2 per cent

Objective:To study the effects of unilateral posterior crossbite on masticatory muscle functions pattern by using electromyography(EMG).Methods:20 subjects(11 males and 9 females)with unilateral posterior crossbite were selected.Subjects were instructed to stay mandibular postural position and to make maximum bite in intercuspal position(ICP)and bilateral chewing.EMG data of the left and right masseter and anterior temporalis muscles were recorded.Asymmetry index of masseter muscles(ASMM) and asymmetry index of temporalis muscles(ASTA)were calculated and compared.20 subjects with individual normal occlusion were used as control.Results:Compared to control group,masseter and temporal muscle activities in unilateral posterior crossbite group were lower in maximum bite and bilateral chewing;The activities of two muscles at crossbite side were lower than that at noncrossbite side.Under maximum bite and bilateral chewing,ASMM and ASTA in unilateral posterior crossbite group were significantly higher than those in control group;ASMM and ASTA in Subjects with one posterior tooth crossbite is lower than subjects with several teeth crossbite.Conclusion:It can be suggested that unilateral posterior crossbite may damage the function of mastication muscle.

Aim To test the strength of Kraekow bilateral locking stitchfor tendon or ligament free end fixation and evaluate the stitch which wasused in reconstruction of cruciate ligament or secure fixation of soft tissue tobone. Methods Twenty-four specimens of patellar tendon free end, whichwere divided into 3 groups according 2 or 3 stitches and 6 subgroups ac-cording No1 Ethilon or 0.4 mm diameter stainless steel wire different suturematerials, fixed with Krackow locking stitch, were undertaken the tensiletest respectively, so as to find out the least stitches and efficient suture pat-tern. Results 2 Krackow locking stitches had more strength than that of0.4 mm diameter stainless steel wire, the fixation strength of 2 stitcheswhich used No1 Ethilon was more than 80 N, superior to the failure strengthof material itself, it still maintained the same strength if the first stitch wasacross the tendon tissuetransversely. There was no significant differencebetween 2 and 3 Krackow locking stitches in suture strength statistically, thefixation strength was superior to the suture material, it could reduce the'suture exposure when the first stitch is across the tendon tissue while main-tains a comparable strength to other suture. Conclusion If attempt to makea higher strength of suture, we should focus on selecting a stronger materialsor adding the suture materials in number rather than the stitch number.

To study the expression of bone morphogetic protein(BMP) and transforming growth factor β１（TGF-β１) in regenerative gap during lengthening period. Method: The right tibia of 14 mature rabbits were lengthened using metaphyseal osteotomy. Distraction was started on the 4th day postoperatively, 1mm per day and conducted twice each day. The expression of endogenesis BMP and TGF-β１ in regeneraive gap were observed during ten weeks after distraction by histology and immunohistochemisty. Results: The result showed that bone morphogetic protein(BMP) and transfoming growth factor β１(TGF-β１) were expressed in chondrocytes, osteoblasts, and fibroblasts. The expression of BMP was at a high level in initial two weeks after distraction while the expression of TGF-β１ was increased step by step. Then both of them decreased alonged with lengthening proceeding. Two weeks after distracting stopped, the expression of BMP could not being observed. But the expression of TGF-β１ could being observed all stage after distraction stopped. Conclusion: BMP and TGF-β１ were expressed persistently in regenerative gap during bone lengthening.

0.05).[Conclusion]Composite of heterogeneous deproteinized bone+autogeneous MSCs+rhBMP2 has the same ability of autogeneous bone graft to repair big experimental segmental tibia defect,thus it can be used as a scaffold in tissue engineering and for further study of immunogenicity and ossification ability.

Objective To establish the measurement for evaluating the efficiency of isolating and harvesting marrow mesenchymal stem cells (MSCs) and observe the main biological characteristic of MSCs in vitro during the passage cultivation. Methods ①The marrow nucleated cells in young rabbit marrow were isolated by the gradient centrifugation and cultivated to isolate and harvest MSCs. The efficiency of isolating and harvesting MSCs was measured by the number of the first passage MSCs from every 10~(6) of the marrow nucleated cells. ②The changes of MSCs morphology, growth and proliferation, synthesis of collagen typeⅠand activity of alkaline phosphatase (ALP) were observed during the passage cultivation. Results ① After cultivated at 2.5?10~(5)/cm~(2) marrow nucleated cells for 9-10 d, the first passage MSCs were harvested. The number of the first passage MSCs had a remarkable statistical positive correlation with the number of the marrow nucleated cells from one rabbit(r=0.932, P

Objective To compare the effects of point contact-dynamic compression plates (PC-DCP) and dynamic compression plates (DCP) on bone structure and mechanical properties of the plated bone in the intact goat tibia model. Methods Ten matured goats were used. The bilateral intact tibia of goats were fixed with DCP and PC-DCP respectively. The goats were sacrificed at 12 and 24 weeks after operation, and the tibiae were harvested. The structure of bone under plate was studied both by CT scan and histomorphology. The mechanical properties of the plated bone were examined by torsional test. Results At 12 weeks after internal fixation, there was obvious osteoporosis in the cortical bone underneath the plate in the DCP group compared with that of the PC-DCP group. Both of the cortical bone thickness underneath the plate and the torsion strength of the plated tibiae were significantly higher in the PC-DCP group than that in DCP group (P0.05). Conclusion The underlying bone resorption after rigid plate fixation is induced by both the disturbance of the bone blood supply and stress shielding. However, the former is the main cause in the early stage of fixation. PC-DCP is able to prevent bone resorption in the early stage of fixation.

Objective To evaluate clinical results of arthroplasty with the UNIVERS 3-D prosthesis in unstable shoulder arthritis. Methods 16 cases, which were 10 males and 6 females aging from 35 to 79 years (mean, 56.3 years), underwent shoulder arthroplasty with the UNIVERS 3-D prosthesis because of unstable arthritis. The patients were evaluated before surgery, at 12 months and the most recent follow-up with an average follow-up of 30.8 months(mean, 12-50 months) by Constant and normalised Constant score, and reviewed by the radiological analysis on preoperative and postoperative X-rays including AP, scapular and axillary views. Constant and normalised Constant score improvements at 12 months and the most recent follow-up evaluation were compared with the preoperative scores using paired-samples t test. Results 1) 8 out of 11(72.7%) cases with mild inferior humeral or glenoid exostosis. 2) The Constant score improved from 43.8?13(normalized Constant score, 51.36%?15.40% ) to 70.6?17.69(normalized Constant score, 86.44%?22.12%). The pain, activity, mobility and strength scores had been improved obviously. 3)Only one case had the radiolucent line around the uncemented humeral component with radiolucent index of less than 1. No any periglenoid component radiolucency, no shift or subsidence in humeral component, no migration and tilt in glenoid component, no change and loosening in the junction bolt between humeral stem and head. 4) Two cases were experienced postoperative complications including impingement of prosthesis with osteophytes, and PE-inlay loosening received revision surgery. Conclusion Excellent clinical results can be obtained by the shoulder arthroplasty using the UNIVERS 3-D prosthesis with low incidence of radiolucency and postoperative complication.