Objective To evaluate the effects of N-acetycysteine on the expression of caveolin-3 (Cav-3) during myocardial ischemia-reperfusion (I/R) in diabetic rats.Methods Twenty-four pathogenfree healthy adult male Sprague-Dawley rats,weighing 230-270 g,were divided into 3 groups (n =8 each) using a random number table:myocardial I/R group (group I/R),diabetes mellitus plus myocardial I/R group (group D) and N-acetycysteine group (group NAC).Diabetes mellitus was induced by injection of streptozotocin 60 mg/kg via the tail vein and confirmed by blood glucose ≥ 16.7 mmol/L 3 days later.At 1 week after successful establishment of the model,N-acetycysteine 1.5 g · kg-1 · d-1 was injected through a gastric tube into stomach for 4 consecutive weeks in group NAC,and the equal volume of normal saline was given for 4 consecutive weeks in I/R and D groups.Myocardial I/R was then induced by 30 min ligation of the left anterior descending branch of the coronary artery followed by 2 h of reperfusion.At the end of reperfusion,the myocardial infarct size was determined by triphenyl tetrazolium chloride staining,the levels of serum creatine kinase-MB (CK-MB) and 15-F2t-Isoprostane were measured by enzyme-linked immunosorbent assay,and the expression of myocardial Cav-3,Akt,phosphor-Akt (p-Akt),endothelial nitric oxide synthase (eNOS) and phosphor-eNOS (p-eNOS) was detected by Western blot.Results Compared with group I/R,the myocardial infarct size and levels of serum CK-MB and 15-F2t-Isoprostane were significantly increased,and the myocardial Cav-3,p-Akt and p-eNOS expression and NO level were decreased in group D (P＜0.05).Compared with group D,the myocardial infarct size and levels of serum CK-MB and 15-F2t-Isoprostane were significantly decreased,and the myocardial Car-3,p-Akt and p-eNOS expression and NO level were increased in group NAC (P＜0.05).Conclusion N-acetycystein can activate Akt/eNOS/NO signaling pathway through up-regulating myocardial Cav-3 expression,thus reducing myocardial I/R injury in diabetic rats.

Objective: To evaluate the long term efficacy of treating the primary dysmenorrhea of cold-damp stagnation type by Acupuncture of Liji therapy. Methods: In this study, a total of 76 cases of primary dysmenorrhea of cold-damp coagulation type were randomly divided into the acupuncture of Liji therapy group, and the body acupuncture group, with38 cases in each group. Both groups were continuously treated 3 menstrual cycles, and followed up in the third and six menstrual cycles after the end of the treatment. Visual analogue scale for abdominal pain and Dysmenorrhea Symptoms scale were used as therapeutic indexes. Remove shedding cases, the long-term effects and scores of the 2 groups were compared. Besides, untoward and side effects needed to be recorded. Results: There were 2 cases lost in the acupuncture ofLiji therapy group and 1 cases in the body acupuncture group. After treatment, The clinical comprehensive efficacy of acupuncture ofLiji therapy group was better than that of body acupuncture group (P ＜ 0. 05) . Both groups of VAS scores and dysmenorrhea symptom scores were decreased to different degrees during treatment and follow-up period (P ＜ 0.05) .The follow-up data of the body acupuncture group after six menstrual cycles were higher than that of the third menstrual cycles after treatment. Compared with the two groups, the acupuncture of Liji therapy group was superior to the body acupuncture group during the third menstrual cycles follow-up (P ＜ 0.05) and six menstrual cycles follow-up (P ＜ 0.01) .The treatment satisfaction of acupuncture of Liji therapy and body acupuncture was 91.67％ and 72.97％. There were no adverse reactions in the two groups during the study period. Conclusion: Acupuncture of Liji therapy can effectively relieve dysmenorrhea symptoms and the general discomfort caused by dysmenorrhea, the long-term effect is stable and durable. primary dysmenorrhea of cold-damp stagnation type with acupuncture of Liji therapy has definitely long term curative effect. In addition, the treatment of patients with acupuncture of Liji therapy is more satisfactory, it is worthy of cilnlcal application.

To summarize the causes of relocation stress in family members of patients transferred from ICU.Based on muhidisciplinary collaboration,stress prevention before transfer and stress intervention after transfer were implemented.For thirty family members of patients in our group,their level of relocation stress 7d after transfer was low.

Objective To explore the status of cognition on hospice care of community health care providers in Changchun City so as to provide an effective reference for developing hospice care in community and relevant departments.Methods From January to March 2017,we extracted three districts(Chaoyang District,Luyuan District,Nanguan District of Changchun City)by convenience sampling and selected 260 eligible community health care providers from 10 community health service centers by chester sampling as respondents.The status of cognition on hospice care of community health care providers was investigated with the self-designed questionnaire.The SPSS 19.0 was used to data analysis.Results The score of knowledge,attitude and behavior on hospice care of community health care providers was(13.26±2.94),(138.29±13.16)and(20.89±3.42)respectively.Conclusions Community health care providers of Changchun City are with a positive attitude toward hospice care.At the same time,they do well in hospice care provided for the dying patients and their family members.However,their grasping state on knowledge of hospice care is weak due to lack systematic professional knowledge training in hospice care.We should strengthen knowledge education as well as training in hospice care for community health care providers and improve the whole cognition on hospice care of community health care providers.

Objective:To explore the status quo of self-management ability in young and middle-aged patients with gout and analyze its influencing factors.Methods:Totally 225 patients with gout admitted in the Department of Rheumatology and the Department of Endocrinology at a ClassⅢ Grade A hospital in Jilin Province from September 2020 to March 2021 were selected by convenient sampling and investigated with the general information questionnaire, Gout Patient Self-Management Assessment Scale, and Medical Coping Modes Questionnaire.Results:The total score of self-management ability in young and middle-aged patients with gout was (127.29±22.42) , including (41.15±7.38) in disease treatment management, (37.41±7.20) in diet management, (27.42±5.62) in lifestyle management, and (21.31±5.06) in psychosocial management. The results of multiple linear regression analysis showed that the education level, course of illness, smoking, facing and avoiding coping styles were the influencing factors of the self-management ability of gout patients ( P<0.05) . Conclusions:The self-management ability of young and middle-aged gout patients is the best in disease treatment management and the worst in psychosocial management in all dimensions, but the overall level is at a medium level and needs to be improved. Clinical medical staff should take targeted health education strategies based on its influencing factors to improve the self-management level of disease in young and middle-aged gout patients.

Humans ; Brain-Computer Interfaces ; Consciousness Disorders ; Consciousness ; Electroencephalography ; Brain

Objective:To explore the effects of intensive nursing intervention on patients with dilated cardiomyopathy and ventricular arrhythmia treated with amiodarone.Methods:Totally 100 patients with dilated cardiomyopathy and ventricular arrhythmia treated with amiodarone who were hospitalized in China-Japan Union Hospital of JiLin University from January 2017 to March 2019 were selected by convenient sampling. Patients hospitalized from January 2017 to February 2018 were divided into the control group, while patients hospitalized from March 2018 to March 2019 were divided into the experimental group. Patients in the control group received routine nursing care, while patients in the experimental group received intensive nursing intervention. The Profile of Mood States-Short Form (POMS-SF) and the World Health Organization Quality of Life (WHOQOL-BREF) were used to evaluate the intervention effects.Results:The POMS-SF scores of the two groups after intervention were lower than those before intervention, and the POMS-SF score of the experimental group was lower than that of the control group, and the differences were statistically significant ( P<0.05) . The WHOQOL-BREF scores of the two groups after intervention were higher than those before intervention, and the WHOQOL-BREF score of the experimental group was higher than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Intensive nursing intervention can mitigate the negative emotion of patients with dilated cardiomyopathy and ventricular arrhythmia treated with amiodarone, and improve their quality of life.