Objective To examine the expression of metastasis-associated in colon cancer 1 (MACC1) gene in ovarian cancer cell lines and investigate its effect on biological behaviors of ovarian cancer cells.Methods The expression of MACC1 was examined by qRT-PCR and Western blot analysis in four ovarian cancer cell lines inculding OVCAR3,ES-2,SKOV3 and HO-8910.When the MACC1 was transfected to OVCAR3 cells,fluorogenic quantitative PCR was used to filter and identify MACC1 gene after the efficient silencing.Changes of adhesion in the cells were analyzed by an adhesion assay.Transwell migration and invasion assay and in vitro vascular mimicry assay were used to detect migration,invasion and angiogenesis of OVCAR3 cells in vitro.Results The expression of MACC1 gene was higher in OVCAR3 compared to other cell lines.qRT-PCR confirmed that the expression of MACC1 was silenced successfully after transient transfected MACC1-siRNA into OVCAR3 cells.After successful silencing the MACC1 expression,the adhesion ability was inhibited to some degree.In transwell migration assay,the numbers of cells in upper chamber passing through the membrane in transfected group were less than control groups (245.5 ±12.8,500.3±16.5 and 496.3±13.1 respectively),while in transwell invasion assay,the numbers of cells in upper chamber passing through the membrane in transfected group were less than the negative group and control group (185.3±14.1,405.7±9.1 and 416.3±11.5 respectively),both with markedly differences among the three groups.In tube formation assay,the distrubition of HUVECs was diffused with less junctions,and the average number of complete tubular structure was decreased in transfected group compared to the corresponding controls.Conclusion RNA interference inhibits the expression of MACC1 and effectively inhibits the metastasis and invasion abilities of ovarian cancer cells in vitro,and MACC1 is expected to become the target gene of ovarian cancer treatment.

Objective To study the clinical application effect of the comprehensive nursing intervention measures in the elderly femoral neck fracture patients with artificial hip arthroplasty (THA), to provide a reference value for clinical nursing intervention. Methods All 46 patients with femoral neck fracture were randomly divided into control group(n=23)and observation group (n=23), the control group was given conventional nursing model, observation group was given comprehensive nursing intervention measures. Comparison of postoperative 8 h, 24 h, 48 hVAS score, preoperative and after postoperative 4 months,12 months, Harris score and postoperative complications were observed. Results The difference of VAS score,Harris score was not statistically significant between the two groups at preoperative(P>0.05);VAS scores in observation group at 8 h, 24 h and 48 h after operation was significantly lower than those in control group,the difference between the two groups was statistically significant (P<0.05),and Harris score in the observation group at 4 months and 12 months postoperative was significantly higher than those of control group, the difference be tween the two groups was statistically significant(P<0.05); postoperative complication rate was 13.0% in the observa tion group,significantly better than the 39.1%in the control group,the difference was statistically significant (P<0.05). Conclusion Comprehensive nursing intervention can significantly decrease the incidence of postoperative complications and improve the postoperative hip joint function and quality of nursing, has important clinical significance, worthy of further dissemination of research.