OBJECTIVE:To investigate the variation degrees of the active components in the same batch No.GuanxinⅡdecoctions under the same decoction technics.METHODS:Crude drug of the same batch No.of the same formula was decocted,every decoction detail was tried to be kept under control,the contents of the active components in 5 batches of GuanxinⅡdecoctions were determined and the mean value of which was calculated as well.RESULTS:In terms of the contents of crude drugs,those of tanshinol,protocatechualdehyde,peoniflorin and ferulic acid were respectively(0.773?0.0 656)mg/g,(36.591?3.0 590)?g/g,(2.655?0.2 454)mg/g,(85.052?7.5 469)?g/g.CONCLUSION:Referenced by the criterion that the content variation coefficient among different batches of decoctions should be less than 10%,the contents of active components in each decoction were all up to the standards.Referenced by the criterion that there should be no statistical difference among different batches of decoctions,more influential factors on the active components of decoctions should be brought into consideration in the quality control.

OBJECTIVE:To study the presence of the chemical compositions of Guan XinⅡ(GXEH)oral decoction in human serum.METHODS:The analysis of finger prints of the medicated serum sample obtained from the volunteers treated with GXEH decoction was conducted with3different pretreatment procedures and2different HPLC methods,and which were compared with decoction.RESULTS:The numbers of the discrepant chromatographic peaks detected from the decoction and the medicated serum were respectively less than30and10,danshensu,protocatechuic aldehyde,ligustrazine,paeoniflorin were failed to be traced in the latter.CONCLUSION:The numbers of chemical compositions of the compound preparation that can be traced in serum were limited and the active components that entered into body may be relatively limited in numbers.After oral administration of compound preparation,a part of chemical compositions of which may not presented as its original form in serum.

OBJECTIVE: To investigate the concentration- time curve of ferulic acid(FA) in serum of healthy male volunteers after oral administration of Guanxin II (GXEH) and to study its pharmacokinetic parameters.METHODS: Serum FA level was determined by HPLC in healthy male volunteers after single oral administration of GXEH(4.5g?kg-1) decoction. RESULTS: No double - peak occurred in the concentration - time curve of FA.6 -fold interindividual variation was noted in maximum blood drug level.The pharmacokinetic parameters were stated as follows: Ka: (0.26?0.350) min-1; Ke: (0.013?0.004 17)min-1;t1/2Ke: (63.45?32.288)min;tmax:(24.117?14.631) min, AUC:(13 263.51?6 478.275)ng?min-1?mL-1; CL/F(s):(0.000 44?0.000 268)L?g-1?min-1.CONCLUSION:There is a significant individual FA level difference in healthy male volunteers after oral oral administration of GXEH decoction,and both the absorption and the elemination of FA are fast.

Objective To investigate the inhibitory effect on enhancement of contraction and relaxation functions of cardiomyocytes induced by of MN9202 TEA, Forskolin, and MB. Methods The twitch amplitude was measured with a video edge tracker method. The following indexes, including ph (peak height), peak height/baseline percent (ph/bl, %), maximal velocity of contraction (+dL/dt) and maximal velocity of diastolization (-dL/dt), were recorded by a computer. Results TEA, Forskolin and MB increased electrically-induced contraction as shown by the following indexes: ph, ph/bl%, +dL/dt and -dL/dt. Forskolin and MB markedly augmented function of cardiac myocyte contraction and diastolization. Comparing with the control group, Forskolin (10-8mol/L) increased ph from 0.12?0.03(um)to 0.24?0.06 (um), ph/bl % from 12?3% to 27?6%, +dL/dt from 1.8?0.5 (um/s) to 3.8?0.9 (um/s), -dL/dt from 1.8?0.22 (um/s) to 3.5?0.7 (um/s). These indexes increased by 91%, 123%, 110% and 89%, respectively. MB also increased significantly; ph, ph/bl %, +dL/dt and -dL/dt were 0.14?0.04um, 11?4%, 2.2?0.3um/s and 2.2?0.6um/s, respectively. MN9202 (3?10~ -6mol/L) decreased the indexes (+dL/dt and -dL/dt ) which were increased by Forskolin significantly. Conclusion TEA, Forskolin and MB increased function of cardiac myocyte contraction and diastolization. These drugs might elevate intracellular calcium content indirectly and directly. The effects of TEA, Forskolin and MB were attenuated by MN9202. MN9202 might not only block Dihydropyridine Receptor, but might also inhibit calcium influx. Further study is needed to elucidate exact mechanism of MN9202.

AIM: To investigate the immunoloregulation effect of Angelica polysaccharide extracted from Angelica Sinensis (Oliv.) Diels. METHODS: Index of immune organs were weighed and calculated. Phagocytosis of mononuclear macrophage (M) were determined with carbon particle clearance test. Spectrophotography was used to estimate levels of serum hemolysis IgG, IgM. The cell proliferation was measured by MTT assay. RESULTS: In certain doses (10-100 mg/kg), Angelica polysaccharide significantly increased phagocytic function and spleen index in immunosuppression mice caused by hydrocortisone, while showed no obviouse influnence on thymus index. It also remarkble decreased levels of serum hemolysis IgG, IgM. In vitro experiments, Angelica polysaccharide could increase the proliferation of mouse spleen cell and macrophage. CONCLUSION: These results suggest that Angelica polysaccharide has potent enhancement on non specific immunity, however, it could suppress humoral immunity.

AIM To get an insight into intracellular signaling steps, a very early step in the signaling cascade, the biphasic Ca 2+ elicited by 5 HT in rat stomach fundus smooth muscle cells was investigated. METHODS Cells were cultured and loaded with Fluo 3 AM. [Ca 2+ ] i was measured by fluorescent intensity (FI) in each cell with confocal microscopy. RESULTS The resting FI level of SFSMC was 264?15. Stimulation of SFSMCs by 5 HT produced an elevation of [Ca 2+ ] i; Depletion of external Ca 2+ by addition of EGTA led to a significant attenuation of [Ca 2+ ] i change induced by 5 HT; Pre treatment of SFSMCs with ryanodine (10 ?mol?L -1 , 5 min) in D Hanks, the effect of 5 HT was completely inhibited; The stimulation of SFSMCs by 5 HT was partly attenuated by miaserin(10 ?mol?L -1 ), however, L type Ca 2+ channel antagonist lacidipine and G protein inhibitor NEM completely abolished the increase of [Ca 2+ ] i mediated by 5 HT; 5 HT mediated Ca 2+ release was reduced by phospholipase C specific inhibitor compound 48/80(1 2 ?g?ml -1 ); When protein kinase C was activated by phorbol 12 myristate 13 acetate (PMA 0 1 ?mol?L -1 , 5 min) the effect of 5 HT was inhibited, and the inhibitory effect of PMA was reversed by D sphingosine, a PKC inhibitor. CONCLUSION Our data suggest that G protein coupled 5 HT 2B receptor in the rat stomach fundus modulates 5 HT stimulated Ca 2+ increase, and it is coupled to calcium influx through L type calcium channels, and also intracellular calcium release by the opening of ryanodine receptor. The 5 HT 2B receptor mediated signal of 5 HT is transduced by PLC and PKC.

AIM To study whether nitric oxide preconditioning induce early protection of neonatal rat cardiomyocytes. METHODS Cultured neonatal rat cardiomyocytes were divided into 6 groups. ①Normal group;②NO group: SNAP(500 ?mol?L -1 ) was added to cell medium 1 h before lethal hypoxia/reoxygenation (HR) injury (6 h hypoxia and 3 h reoxygenation); ③HP group: Cells were cultured for 60min hypoxia followed by 30 min reoxygenation to form hypoxia preconditioning before lethal HR injury;④ L NAME+HP group: Nitric oxide synthase antagonist L NAME was added during HP stage;⑤ L Arg group: L arginine was added to cell medium 1h before lethal HR injury;⑥ L NAME+ L Arg group: Both L NAME and L Arg were added to cell medium 1 h before lethal HR injury;⑦H/R group: Cells underwent lethal HR injury without any treatment. Cardiomyocytes injury was detected by lactate dehydrogenase(LDH) activity and cell viability. RESULTS Nitric oxide preconditioning can protect cardiomyocytes by reducing LDH activity and improving cell viability ( P

AIM To investigate the immunolore gulation effect of Angelica polysaccharide purified from Angelica sinensis(Oliv.)Diels. METHODS Index of immune organs was weighed and caculated. Phagocytosis of mononuclear macrophage (M?) was determined with carbon particle clearance test. Spectrophotography was used to estimate levels of serum hemolysis IgG, IgM. Mixed lymphocyte reaction was measured by MTT assay. RESULTS In certain concentrations (10～100 mg?kg -1 ), Angelica polysaccharide significantly in creased phagocytic function and spleen index inboth normal and immunosuppression mice,caused by sc cyclophosphamide, but showed no obviouse influnence on thymus index. It also remarkbly decreased levels of serum hemolysis IgG, IgM. CONCLUSION These results suggest that Angelica polysaccharide has potent enhancement on non specific immunity. However, it could suppress humoral immunity.

This paper summarised related investigations, literature and theoretical and experimental achievements in the field, describing key technology of the methodology of F P of TCMs. The scientific, progressive, practical and feasible significance is discussed.