OBJECTIVE:To investigate the variation degrees of the active components in the same batch No.GuanxinⅡdecoctions under the same decoction technics.METHODS:Crude drug of the same batch No.of the same formula was decocted,every decoction detail was tried to be kept under control,the contents of the active components in 5 batches of GuanxinⅡdecoctions were determined and the mean value of which was calculated as well.RESULTS:In terms of the contents of crude drugs,those of tanshinol,protocatechualdehyde,peoniflorin and ferulic acid were respectively(0.773?0.0 656)mg/g,(36.591?3.0 590)?g/g,(2.655?0.2 454)mg/g,(85.052?7.5 469)?g/g.CONCLUSION:Referenced by the criterion that the content variation coefficient among different batches of decoctions should be less than 10%,the contents of active components in each decoction were all up to the standards.Referenced by the criterion that there should be no statistical difference among different batches of decoctions,more influential factors on the active components of decoctions should be brought into consideration in the quality control.

OBJECTIVE:To study the presence of the chemical compositions of Guan XinⅡ(GXEH)oral decoction in human serum.METHODS:The analysis of finger prints of the medicated serum sample obtained from the volunteers treated with GXEH decoction was conducted with3different pretreatment procedures and2different HPLC methods,and which were compared with decoction.RESULTS:The numbers of the discrepant chromatographic peaks detected from the decoction and the medicated serum were respectively less than30and10,danshensu,protocatechuic aldehyde,ligustrazine,paeoniflorin were failed to be traced in the latter.CONCLUSION:The numbers of chemical compositions of the compound preparation that can be traced in serum were limited and the active components that entered into body may be relatively limited in numbers.After oral administration of compound preparation,a part of chemical compositions of which may not presented as its original form in serum.

OBJECTIVE: To investigate the concentration- time curve of ferulic acid(FA) in serum of healthy male volunteers after oral administration of Guanxin II (GXEH) and to study its pharmacokinetic parameters.METHODS: Serum FA level was determined by HPLC in healthy male volunteers after single oral administration of GXEH(4.5g?kg-1) decoction. RESULTS: No double - peak occurred in the concentration - time curve of FA.6 -fold interindividual variation was noted in maximum blood drug level.The pharmacokinetic parameters were stated as follows: Ka: (0.26?0.350) min-1; Ke: (0.013?0.004 17)min-1;t1/2Ke: (63.45?32.288)min;tmax:(24.117?14.631) min, AUC:(13 263.51?6 478.275)ng?min-1?mL-1; CL/F(s):(0.000 44?0.000 268)L?g-1?min-1.CONCLUSION:There is a significant individual FA level difference in healthy male volunteers after oral oral administration of GXEH decoction,and both the absorption and the elemination of FA are fast.

Objective To investigate the inhibitory effect on enhancement of contraction and relaxation functions of cardiomyocytes induced by of MN9202 TEA, Forskolin, and MB. Methods The twitch amplitude was measured with a video edge tracker method. The following indexes, including ph (peak height), peak height/baseline percent (ph/bl, %), maximal velocity of contraction (+dL/dt) and maximal velocity of diastolization (-dL/dt), were recorded by a computer. Results TEA, Forskolin and MB increased electrically-induced contraction as shown by the following indexes: ph, ph/bl%, +dL/dt and -dL/dt. Forskolin and MB markedly augmented function of cardiac myocyte contraction and diastolization. Comparing with the control group, Forskolin (10-8mol/L) increased ph from 0.12?0.03(um)to 0.24?0.06 (um), ph/bl % from 12?3% to 27?6%, +dL/dt from 1.8?0.5 (um/s) to 3.8?0.9 (um/s), -dL/dt from 1.8?0.22 (um/s) to 3.5?0.7 (um/s). These indexes increased by 91%, 123%, 110% and 89%, respectively. MB also increased significantly; ph, ph/bl %, +dL/dt and -dL/dt were 0.14?0.04um, 11?4%, 2.2?0.3um/s and 2.2?0.6um/s, respectively. MN9202 (3?10~ -6mol/L) decreased the indexes (+dL/dt and -dL/dt ) which were increased by Forskolin significantly. Conclusion TEA, Forskolin and MB increased function of cardiac myocyte contraction and diastolization. These drugs might elevate intracellular calcium content indirectly and directly. The effects of TEA, Forskolin and MB were attenuated by MN9202. MN9202 might not only block Dihydropyridine Receptor, but might also inhibit calcium influx. Further study is needed to elucidate exact mechanism of MN9202.

AIM: To investigate the immunoloregulation effect of Angelica polysaccharide extracted from Angelica Sinensis (Oliv.) Diels. METHODS: Index of immune organs were weighed and calculated. Phagocytosis of mononuclear macrophage (M) were determined with carbon particle clearance test. Spectrophotography was used to estimate levels of serum hemolysis IgG, IgM. The cell proliferation was measured by MTT assay. RESULTS: In certain doses (10-100 mg/kg), Angelica polysaccharide significantly increased phagocytic function and spleen index in immunosuppression mice caused by hydrocortisone, while showed no obviouse influnence on thymus index. It also remarkble decreased levels of serum hemolysis IgG, IgM. In vitro experiments, Angelica polysaccharide could increase the proliferation of mouse spleen cell and macrophage. CONCLUSION: These results suggest that Angelica polysaccharide has potent enhancement on non specific immunity, however, it could suppress humoral immunity.

To observe the expression of cyclooxygenase (COX)-1 and COX-2 in brain after spared nerve injury (SNI) and compare the analgesic effects of COX inhibitors with different selectivity. Radioimmunoassay, RT-PCR and Western blotting techniques were used to evaluate the change of brain COX expression at different time points( before SNI, 1 h, 12 h, 1 d, 3 d, 7 d, 14 d, 30 d and 60 d after SNI); By exploring hot plate test, we observed the reacting time of animals after injection of saline, NS-398, SC-560 and indomethacin at different time points. The results showed that: ( 1 ) The expression of brain COX-1 didn't increase significantly until 14 d after SNI, while that of COX-2 increased significantly and rapidly after SNI and reached peak at the time point of 1 d ( all P ＜0.05 ); (2) NS-398 showed significant analgesic effect on neuropathic pain after SNI at the early phase ( P ＜ 0.05 ), but didn't persist for over 30 d; ( 3 ) Indomethacin and SC-560 didn't show significant analgesic effects until 14 d. These results suggest that brain COX-1 is involved in the late phase of neuropathic pain and may play a role in the persistence of pain, while brain COX-2 is involved in the early phase of neuropathic pain and may play a role in the pain origination.

BACKGROUND: How to choose traditional Chinese remedy to treat cerebrovascular disease, not only improving the brain blood supply but also not affecting the blood pressure and heart rate, has been a promising research.OBJECTIVE: To study the role of yangyintongnao granule on average blood pressure and heart rate in anaesthetized dog.DESIGN: Complete grouping design and randomized controlled study based on hybrid dog.SETTING: Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University of Chinese PLA and Department of Pharmacology, Faculty of Preclinical Medicine, Fourth Military Medical University of Chinese PLA MATERIALS: The experiment had finished by Cardiovascular Laboratory of Physiology Department in the Fourth Military Medical University of Chinese PLA from March to June 2003. Totally 23 healthy hybrid dogs in either sex were selected. These dogs were divided randomly into 4 groups: high dose group of yangyintongnao( n = 8), moderate dose group of yangyintongnao ( n = 6), low dose group of yangyintongnao( n = 5) and saline group( n = 4).METHODS: Anesthetic dogs in high, moderate and low dose group of yangyintongnao granule were given different doses of yangyintongnao granule: 2 g/kg, 1 g/kg and 0. 5 g/kg respectively. All doses of drug were calculated based on the body mass of dogs, and the drugs were dissolved in 100 mL saline and given through the gastric canal. Dogs in saline group were perfused with equal saline. Aortic average blood pressure was measured by femoral artery intubation via a piezometric transducer. Heart rate was obtained from R-R intervals of a standard Ⅱ lead of electrocardiograph(ECG). The mean blood pressure and heart rate were recorded 0. 5, 1, 1.5, 2.0,3.0, 4. 0, 5.0, 6. 0 hours after treatment.MEAN OUTCOME MEASURES: The changes of blood pressure and heart rate at different time before and after medication.RESULTS: Totally 29 dogs were brought into the final analysis. Blood pressure: The mean blood pressure was reduced -5.4% to -6. 2% respectively in high dose group and moderate dose group after treatment. But in low dose group the average blood pressure sometimes increased sometimes decreased, mainly decreased. It increased by 6.6% ( P ＞ 0. 05) and decreased by -4. 1% ( P ＞ 0.05) at maximum. The average blood pressure in saline group changed by - 9.6% ( P ＞ 0.05). Heart rate: The heart rate in high and medium dose group gradually reduced as time went on. It reduced by - 4. 4%, - 12.2% and - 9.5% respectively in high, moderate and low doses group. The change in each group was not statistically significant as the same in saline group( P ＞ 0.05).CONCLUSION: Yangyintongnao granule has no significant influence on average blood pressure and heart rate.

AIM To get an insight into intracellular signaling steps, a very early step in the signaling cascade, the biphasic Ca 2+ elicited by 5 HT in rat stomach fundus smooth muscle cells was investigated. METHODS Cells were cultured and loaded with Fluo 3 AM. [Ca 2+ ] i was measured by fluorescent intensity (FI) in each cell with confocal microscopy. RESULTS The resting FI level of SFSMC was 264?15. Stimulation of SFSMCs by 5 HT produced an elevation of [Ca 2+ ] i; Depletion of external Ca 2+ by addition of EGTA led to a significant attenuation of [Ca 2+ ] i change induced by 5 HT; Pre treatment of SFSMCs with ryanodine (10 ?mol?L -1 , 5 min) in D Hanks, the effect of 5 HT was completely inhibited; The stimulation of SFSMCs by 5 HT was partly attenuated by miaserin(10 ?mol?L -1 ), however, L type Ca 2+ channel antagonist lacidipine and G protein inhibitor NEM completely abolished the increase of [Ca 2+ ] i mediated by 5 HT; 5 HT mediated Ca 2+ release was reduced by phospholipase C specific inhibitor compound 48/80(1 2 ?g?ml -1 ); When protein kinase C was activated by phorbol 12 myristate 13 acetate (PMA 0 1 ?mol?L -1 , 5 min) the effect of 5 HT was inhibited, and the inhibitory effect of PMA was reversed by D sphingosine, a PKC inhibitor. CONCLUSION Our data suggest that G protein coupled 5 HT 2B receptor in the rat stomach fundus modulates 5 HT stimulated Ca 2+ increase, and it is coupled to calcium influx through L type calcium channels, and also intracellular calcium release by the opening of ryanodine receptor. The 5 HT 2B receptor mediated signal of 5 HT is transduced by PLC and PKC.