1.Expression of Toll-like receptor 4 and protein of PI3 K/AKT signal pathway in cervical lesion and their relationship with HPV 16 infection
Haiyan SHI ; Yanli ZUO ; Qirui LIN ; Qiaozhi LI ; Weiwei HU
Chinese Journal of Clinical and Experimental Pathology 2014;(11):1232-1236
Purpose To explore the expression of Toll-like receptor 4 (TLR4), PI3K, AKT and NF-κB in cervical lesions, and to in-vestigate their association with human papillomavirus ( HPV) 16 infection. Methods Immunohistochemical SP staining was performed to detect the expression of TLR4, PI3K, AKT, NF-κB in paraffin-embedded cervical tissue specimens from Uighur women with chroni-cal cervicitis, cervical intraepithelial neoplasia ( CIN) and cervical squamous cell carcinoma ( CSCC) . The HPV 16 DNA was detected by PCR. Results The positive expression rates of TLR4, PI3K, AKT, NF-κB in chronical cervicitis, CIN and cervical cancer were 32. 0%, 59. 4%, 77. 8%, 28. 0%, 56. 3%, 73. 0%, 24. 0%, 56. 3%, 79. 4%, and 8. 0%, 48. 4%, 81. 0%, respectively. The expression of them was higher in cervicitis than in CIN and cevical cancer ( P<0. 05 ) . The positive expression rates of HPV 16 in three groups were 8. 0%, 48. 4% and 81. 0% (P<0. 05). The expression of TLR4, PI3K, NF-κB and HPV 16 was related to cervi-cal cancer differentiation (P<0. 05). PI3K and AKT were significantly correlated with FIGOs’ stages (P<0. 05). NF-κB was corre-lated with lymph node metastasis. The expression of TLR4 was significantly associated with HPV 16 infection in CIN and CSCC ( r=0. 303, P=0. 015, r=0. 633, P=0. 000), and correlation with PI3K in CIN and CSCC (r=0. 254, P=0. 045, r=0. 386, P=0. 003). PI3K was associated with AKT only in CSCC (r=0. 298, P=0. 018). Conclusions The expression of TLR4 can be up-regulated by HPV 16 infection. High expression of PI3K/AKT signal pathway mediated by TLR4 may play important roles in the devel-opment and progression of CIN and CSCC, and HPV 16 infection may be a trigger factor affecting the molecular signal pathway.
2.Investigation on the Normal Reference Range of Vein Blood Cell for Adults in Shenyang District
China Modern Doctor 2009;47(18):193-194
Objective To establish normal reference range of vein blood cell for adults in shenyang district. Methods Detected the blood cell on the 4218 healthy adults using automatic hemocyte analyzer MEK6318K and analysed the results,the sexual difference.Resultts The blood cell parameters of the blood cell in healthy adults have obvious difference in gender(P<0.01). RBC: male 5.03×1012/L,female 4.60 ×1012/L HBG:male 152.45 × g/L,female 138.28 x g/L. HCT:male 0.444 ×:L/L,female 0.387 × L/L WBC:male 5.99 ×10 9/L,female 6.14 × 109/L. Conclusion The blood cell parameters difference in shenyang is distinctly.
3.Multicenter research on the compliance of clinical pathway of bronchopneumonia in pediatrics of tertiary class A hospitals
Rou LIU ; Kexin SHUAI ; Yanmin BAO ; Jing LI ; Lihua LIN ; Jizu LING ; Li QIU ; Xueyan WANG ; Zhengkun XIA ; Qiaozhi YANG ; Lei ZHANG ; Man ZHANG ; Zhou FU ; Baoping XU
Chinese Journal of Applied Clinical Pediatrics 2020;35(16):1225-1229
Objective:To evaluate the enrollment rate, mutation rate and causes of variability the clinical pathway of bronchopneumonia.Methods:The enrollment rate, completion rate, variation and reasons of the clinical pathway in Beijing Children′s Hospital, Capital Medical University from January 2012 to December 2016 were retrospectively collected.Data of patients after the clinical pathway of bronchopneumonia in other tertiary class A hospitals were gathered by questionnaires, and the enrollment rate, completion rate, variation rate and reasons were analyzed.Results:(1)At the end of 2016, 11 of the 13 hospitals included in this study had implemented the clinical pathway for 5 years, 1 hospital for 3 years, and 1 hospital for 2 years.(2) Eleven hospitals provided their enrollment rates.The enrollement rate of 2 hospitals was<50%, and that of 9 hospitals was>80%.The annual completion rate of Beijing Children′s Hospital was ≥75%, and the completion rates offered by 8 hospitals were basically >70%.(3) Since the implementation of the clinical pathway for 5 years in Beijing Children′s Hospital, a total of 427 cases were enrolled of which 93 cases were mutated (variability 21.78%). The variability of 5 hospitals was maintained at <15%.The variability of 3 hospitals decreased with the implementation years, and became qualified.The variability of 1 hospital first rebounded and then controlled; 1 hospital increased by 27.65%; 1 hospital was first controlled and rebounded; 1 hospital was always >15%.The main cause of the mutation was coexisting diseases, complications, progression of the disease, or correction of the first diagnosis, etc.Conclusions:The completion rate of tertiary class A hospitals meets the requirements of national policy.However, the enrollment rate needs to be improved, and the variation rate among different hospitals differs a lot.Further implementation of the clinical pathway should be strengthened and monitored.
4. A multicenter clinical study on 1 138 cases of invasive pneumococcal disease in children from 2012 to 2017
Liang ZHU ; Wenhui LI ; Xinhong WANG ; Kun TAN ; Qingfeng FANG ; Qingxiong ZHU ; Kangkang WU ; Qiaozhi YANG ; Aiwei LIN ; Huiling DENG ; Jing BI ; Jing LIU ; Shiyong ZHAO ; Yun LIU ; Shujun JING ; Yumin WANG ; Lianmei LI ; Qing ZHAO ; Kaihu YAO ; Xi WANG ; Li JIA ; Fang WANG ; Jikui DENG ; Jing SUN ; Chunhui ZHU ; Kai ZHOU ; Jun LIANG ; Xiuzhen NIE ; Sancheng CAO ; Dongmeng WANG ; Shuangjie LI ; Xuexia CHEN ; Juan LI ; Yi WANG ; Lan YE ; Yanhong ZHANG ; Fang DONG ; Zhi LI ; Yonghong YANG ; Gang LIU
Chinese Journal of Pediatrics 2018;56(12):915-922
Objective:
To explore the clinical features, the serotype distribution and drug resistance of the isolates in patient with invasive pneumococcal disease (IPD).
Methods:
By retrieving the laboratory information system in 18 children′s hospitals from 2012 to 2017, the children with IPD were enrolled.