OBJECTIVE:To observe the effects as well as adverse durgs reactions of chemotherapy involving oxaliplatin in treatment of advanced nasopharyngeal carcinoma(NPC).METHODS:28patients with advanced NPC underwent chemotherapy with5-Fu,tetrahydrofolic acid calcium,and oxaliplatin for21days as one cycle,the therapeutic effects were evaluated after two cycles.Meanwhile the retrospective comparative study was made between the chemotherapy and the regular chemotherapy with5-Fu,tetrahydrofolic acid calcium,and platinum.RESULTS:Similar effects were observed in both regimens.How?ever,there were great differences in incidence ofⅢandⅣgrade nausea and vomiting,as well as renal function indices(P

To meet the need of cultivating high-quality personnnel,enhance the medicalstudents' ability to analyze and solve problems,directing at the problems existed in pharmacology experiment,we have adjusted and reformed the content of the experiment teaching in pharmacology and conducted the survey and research among the students so as to lay a solid fundation to its reasonable implement.

OBJECTIVETo ascertain whether a coding mutation (Ile93Met) in ubiquitin carboxy-terminal hydrolase (UCH-L1) gene plays a role in idiopathic Parkinson's disease (IPD).

METHODSPolymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP) was used to distinguish the wild-type (two DNA fragments of 34 and 126 bp) from the variant allele (three fragments of 34, 60 and 66 bp) because the mutation created a new site for restriction endonuclease BsmF1. DNA was isolated from various blood samples using a phenolchloroform extraction.

RESULTSIle93Met substitution was found neither in PD patients nor in controls.

CONCLUSIONSOur study suggested that Ile93Met of UCH-L1 gene did not influence risk of IPD.

Aged ; Amino Acid Substitution ; Female ; Humans ; Male ; Middle Aged ; Mutation ; Parkinson Disease ; genetics ; Thiolester Hydrolases ; genetics ; physiology ; Ubiquitin Thiolesterase

Objective To improve the recognition and management of primary breast lymphoma(PBL) by discussing the clinical pathological characteristics and the prognosis of PBL. Methods The clinical and treatment records of PBL pa-tients admitted in Boai hospital of zhongshan from January 2001 to December 2012 were analyzed. Results All cases were B-cell origin(25/25),61.5%(16/25)were diffuse large B-cell lymphoma origin,The 5-year overall survival was 44%. There were no significant difference in the 5-year overall survival rates, 5-year local control rates and 5-year distant control rates in patient treatment with any surgery compared with no-Surgery(P>0.05).There were no significant difference in the 5-year overall survival rates and 5-year distant control rates in patient treatment with any RT com-pared with no-RT(P>0.05),but the 5-year local control rates was significantly different(P<0.05);There were no sig-nificant difference in the 5-year overall survival rates and 5-year local control rates in patient treatment with any chemotherapy compared with no-chemotherapy (P>0.05). but the 5-year distant control rates was significant different(P<0.05). Conclusion The prognosis of PBL is related with the clinical stage and pathological type. Radiotherapy and chemotherapy respectively promote the local control and distant control. The optimal treatment is surgery combined with chemotherapy and/or radiotherapy.

Exposure to ionizing radiation intervenes in genomic stability and gene expression,resulting in the disruption of normal metabolic processes in cells and organs by causing complex biolog-ical responses.Altered genomic variations,gene expression and metabolite concentrations in blood or tissue samples reflect systemic radiation damage.With the application of new techniques and exten-sive study on the mechanisms for ionizing radiation damage,related indicators such as chromosomal variation,gene expression,lipid and metabolism are being recognized and promise to be the markers for early diagnosis and prognosis of radiation exposure.Therefore,this article reviews recent progress in and potential applications of biomarkers related to ionizing radiation injury.

Objective To investigate occupational stress in migrant workers in an electronics manufacturing service enterprise and the association between occupational stress and early health effects,such as job burnout,depressive tendency,and insomnia.Methods In August 2015,stratified random cluster sampling was used to select 1 097 migrant workers in an electronics manufacturing service enterprise.The Job DemandAutonomy Questionnaire and effort-reward imbalance questionnaire were used to investigate occupational stress with the types of high workload and effort-reward imbalance,and Burnout Inventory,depression scale,and selfmanagement sleep questionnaire were used to investigate the early health effects of occupational stress.Results In these migrant workers,the detection rates of occupational stress with the types of high workload and effortreward imbalance were 69.8％ (766/1 097) and 11.9％(131/1 097).The multivariate logistic regression analysis showed that the workers who had occupational stress with the types of high workload and effort-reward imbalance had significantly higher risks of job burnout and depressive tendencies than those who did not have these two types of occupational stress (P＜0.01).The workers who had occupational stress with the type of effort-reward imbalance had a significantly higher ability to predict the risks of job burnout and depressive tendencies than those who had occupational stress with the type of high workload(P＜0.01),as well as a significantly higher risk of insomnia than those who did not have this type of occupational stress (P＜0.01).Conclusion Occupational stress is the major influencing factor for early health effects,such as job burnout,depressive tendency,and insomnia,in the migrant workers in this electronics manufacturing service enterprise.The workers who have occupational stress with the type of effort-reward imbalance have higher risks of job burnout and depressive tendencies than those who have occupational stress with the type of high workload.

Objective To investigate occupational stress in migrant workers in an electronics manufacturing service enterprise and the association between occupational stress and early health effects,such as job burnout,depressive tendency,and insomnia.Methods In August 2015,stratified random cluster sampling was used to select 1 097 migrant workers in an electronics manufacturing service enterprise.The Job DemandAutonomy Questionnaire and effort-reward imbalance questionnaire were used to investigate occupational stress with the types of high workload and effort-reward imbalance,and Burnout Inventory,depression scale,and selfmanagement sleep questionnaire were used to investigate the early health effects of occupational stress.Results In these migrant workers,the detection rates of occupational stress with the types of high workload and effortreward imbalance were 69.8％ (766/1 097) and 11.9％(131/1 097).The multivariate logistic regression analysis showed that the workers who had occupational stress with the types of high workload and effort-reward imbalance had significantly higher risks of job burnout and depressive tendencies than those who did not have these two types of occupational stress (P＜0.01).The workers who had occupational stress with the type of effort-reward imbalance had a significantly higher ability to predict the risks of job burnout and depressive tendencies than those who had occupational stress with the type of high workload(P＜0.01),as well as a significantly higher risk of insomnia than those who did not have this type of occupational stress (P＜0.01).Conclusion Occupational stress is the major influencing factor for early health effects,such as job burnout,depressive tendency,and insomnia,in the migrant workers in this electronics manufacturing service enterprise.The workers who have occupational stress with the type of effort-reward imbalance have higher risks of job burnout and depressive tendencies than those who have occupational stress with the type of high workload.

Objective:To analyze the mediating effect of psychological capital (PsyCap) between occupational stress and suicidal ideation.Methods:In September 2017, stratified-cluster sampling method was adopted to select employees of an electronic manufacturing service industry as respondents for a questionnaire survey in Jiangsu province. The demographic characteristics of employees were collected. A total of 2 465 employees were investigated, and 2 216 valid questionnaires were collected. The level of occupational stress and PsyCap was assessed by the Effort-Reward Imbalance Scale(ERI)and PsyCap Questionnaire.Results:The age of 2 216 employees was (28.70±5.26) years old, including 1 230 males (55.5%). The scores of occupational stress on effort, reward and over-commitment were (3.08±0.63), (3.73±0.60) and (2.51±0.68), respectively. The average ERI index (ratio of effort to return) was (0.88±0.39). The total score of PsyCap was (86.62±15.23). The detection rate of suicidal ideation within two weeks was 13.9% (309/2 216). The average score of effort and over-commitment and the ERI index were positively correlated with the number of suicidal ideation detected within two weeks, with correlation coefficients about 0.081, 0.141 and 0.164, respectively. The average score of reward and total score of PsyCap were negatively correlated with the number of suicidal ideation detected within two weeks, with correlation coefficients about -0.206 and -0.228, respectively. The mediating effect results showed that PsyCap had mediating effect on the effort, reward, over-commitment and suicidal ideation of occupational stress respectively, with mediating effect values of -0.087 (95% CI: -0.018,-0.019), -0.299 (95% CI: -0.428,-0.200) and 0.096 (95% CI: 0.0394, -0.170). The mediating effect percentages were 9.43%, 17.92%, and 9.54%, respectively. Conclusion:PsyCap has a mediating effect between effort, reward, over-commitment of occupational stress and suicidal ideation.

Objective:To analyze the mediating effect of psychological capital (PsyCap) between occupational stress and suicidal ideation.Methods:In September 2017, stratified-cluster sampling method was adopted to select employees of an electronic manufacturing service industry as respondents for a questionnaire survey in Jiangsu province. The demographic characteristics of employees were collected. A total of 2 465 employees were investigated, and 2 216 valid questionnaires were collected. The level of occupational stress and PsyCap was assessed by the Effort-Reward Imbalance Scale(ERI)and PsyCap Questionnaire.Results:The age of 2 216 employees was (28.70±5.26) years old, including 1 230 males (55.5%). The scores of occupational stress on effort, reward and over-commitment were (3.08±0.63), (3.73±0.60) and (2.51±0.68), respectively. The average ERI index (ratio of effort to return) was (0.88±0.39). The total score of PsyCap was (86.62±15.23). The detection rate of suicidal ideation within two weeks was 13.9% (309/2 216). The average score of effort and over-commitment and the ERI index were positively correlated with the number of suicidal ideation detected within two weeks, with correlation coefficients about 0.081, 0.141 and 0.164, respectively. The average score of reward and total score of PsyCap were negatively correlated with the number of suicidal ideation detected within two weeks, with correlation coefficients about -0.206 and -0.228, respectively. The mediating effect results showed that PsyCap had mediating effect on the effort, reward, over-commitment and suicidal ideation of occupational stress respectively, with mediating effect values of -0.087 (95% CI: -0.018,-0.019), -0.299 (95% CI: -0.428,-0.200) and 0.096 (95% CI: 0.0394, -0.170). The mediating effect percentages were 9.43%, 17.92%, and 9.54%, respectively. Conclusion:PsyCap has a mediating effect between effort, reward, over-commitment of occupational stress and suicidal ideation.

G protein-coupled receptors (GPCRs) are involved in all human physiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect downstream cascades to impact both health and disease states. Yet, despite their importance as therapeutic targets, detailed molecular structures of only 30 GPCRs have been determined to date. A key challenge to their structure determination is adequate protein expression. Here we report the quantification of protein expression in an insect cell expression system for all 826 human GPCRs using two different fusion constructs. Expression characteristics are analyzed in aggregate and among each of the five distinct subfamilies. These data can be used to identify trends related to GPCR expression between different fusion constructs and between different GPCR families, and to prioritize lead candidates for future structure determination feasibility.
Animals ; Computational Biology ; Crystallography, X-Ray ; Gene Expression ; Humans ; Plasmids ; genetics ; metabolism ; Protein Domains ; Receptors, Adrenergic, beta-1 ; Receptors, G-Protein-Coupled ; classification ; genetics ; metabolism ; Receptors, Odorant ; metabolism ; Receptors, Purinergic P1 ; genetics ; metabolism ; Sf9 Cells ; Spodoptera