A pair of specific primers was designed based on the reported Bm86 gene of Boophilus microplus,the Bm86 gene was cloned by PCR using the plasmid pMD18-T-Bm86 as templates,and subcloned into the prokaryotic plasmid pGEX-4T-1.The recombined plasmid was transformed into E.coli BL21(DE3) and followed by expression of the protein induced by different concentration of IPTG for different time.SDS-PAGE showed that the recombinant plasmid pGEX-4T-1/Bm86 expressed a fusion protein Bm86-GST(Mr 94 000) after being induced with IPTG.High level expre-ssion of Bm86-GST was found at 1 mmol/L IPTG condition after incubation for 8 h at 37 ℃,and the expression level of the recom-binant Bm86-GST reached up to 29% of total E.coli proteins.Western-blotting analysis showed that the recombinant Bm86-GST was recognized by the rabbit anti-B.microplus positive serum.

Objective:To investigate the value of chain management of perioperative pressure injury in patients undergoing supine craniocerebral injury, so as to provide reference for the prevention of pressure injury and the application of chain management.Methods:In this retrospective study, a total of 116 patients with craniocerebral injury who underwent supine surgery in Shanxi Cardiovascular Hospital from December 2019 to December 2020 were included in the control group, using traditional perioperative pressure injury management protocol. And 124 patients admitted from January 2021 to December 2021 were included in the observation group, and were managed by the pressure injury chain management scheme. The risk assessment/judgment rate of pressure ulcers, the risk assessment/judgment accuracy rate of pressure ulcers, the implementation rate of intervention measures, the qualified rate of intervention records and the reported rate of high-risk patients with pressure ulcers in the two groups were analyzed. The occurrence of stress injury and medical expenses of the two groups of patients were investigated.Results:The risk assessment/judgment rate of pressure ulcers, the risk assessment/judgment accuracy rate of pressure ulcers, the implementation rate of intervention measures, the qualified rate of intervention records and the reported rate of high-risk patients with pressure ulcers were 100.00%(124/124), 96.77%(120/124), 92.74%(115/124), 93.55%(116/124), 100.00%(29/29) in the observation group, which were 77.59%(90/116), 72.41%(84/116), 68.97%(80/116), 68.97%(80/116), 83.33%(30/36) in the control group, the difference was statistically significant ( χ2 values were 22.24-31.17, all P<0.05). The overall incidence of stress injury in the observation group and the control group was 15.52%(18/116) and 3.23%(4/124), respectively, and the difference between the two groups was statistically significant ( χ2=10.88, P<0.05). There was no significant difference in the total hospitalization cost between the two groups ( P>0.05). The cost of stress-related consumables was (144.94 ± 47.28) yuan in the observation group and (194.21 ± 55.76) yuan in the control group, and the difference was statistically significant ( t=7.40, P<0.05). Conclusions:Perioperative stress injury chain management can reduce the incidence of pressure injury in patients with craniocerebral injury, improve service quality, and reduce medical costs, with good clinical application value.

In order to determine the effect of various hosts on feeding performance of Rhipicephalus (Boophilus) microplus, we used 3 mammalian species as hosts, cattle (Qinchuan), sheep (T an), and rabbits (Japanese white rabbit) for infest-ing ticks. Five hundreds of R. microplus larvae were exposed to each animal (3 animals/host species). Tick recoveries were 11.0%, 0.47%, and 5.5% from cattle, sheep, and rabbits, respectively. The averages of tick feeding periods were not significantly different on cattle, sheep, and rabbits, 28.8, 25.3, and 26.7 days, respectively. The average weights of individual engorged female from cattle, sheep, and rabbits were 312.5, 219.1, and 130.2 mg, respectively and those of egg mass weights each to 85.0, 96.6, and 17.8 mg. The highest egg hatching rate was in the ticks from cattle (96.0%), fol-lowed by those from rabbits (83.0%) and sheep (19.2%). These data suggest that rabbits could be as an alternative host to cultivate R. microplus for evaluating vaccines and chemical and biological medicines against the tick in the laboratory, although the biological parameters of ticks were less than those from cattle.
Animals ; Cattle* ; Female ; Humans ; Larva ; Ovum ; Rabbits* ; Rhipicephalus* ; Sheep* ; Ticks ; Vaccines ; Weights and Measures

Species identification using DNA sequences is the basis for DNA taxonomy. In this study, we sequenced the ribosomal large-subunit RNA gene sequences (3,037-3,061 bp) in length of 13 Chinese Theileria stocks that were infective to cattle and sheep. The complete 28S rRNA gene is relatively difficult to amplify and its conserved region is not important for phylogenetic study. Therefore, we selected the D2-D3 region from the complete 28S rRNA sequences for phylogenetic analysis. Our analyses of 28S rRNA gene sequences showed that the 28S rRNA was useful as a phylogenetic marker for analyzing the relationships among Theileria spp. in ruminants. In addition, the D2-D3 region was a short segment that could be used instead of the whole 28S rRNA sequence during the phylogenetic analysis of Theileria, and it may be an ideal DNA barcode.
Animals ; Base Sequence ; China ; DNA, Ribosomal/chemistry/genetics ; Molecular Sequence Data ; Phylogeny ; RNA, Ribosomal, 28S/genetics ; Ruminants ; Sequence Alignment ; Sequence Analysis, DNA/veterinary ; Theileria/*classification/genetics/isolation & purification ; Theileriasis/*parasitology

ObjectiveTo explore the psychological and behavioral characteristics of understanding Chinese garden path sentences in children with high-functioning autism (HFA). MethodsFrom April to August, 2019, 35 children with HFA and 35 age-matched typically developing (TD) children were recruited by social recruitment method in the Key Laboratory of Speech and Hearing of East China Normal University in Shanghai. The Garden Path Sentence Comprehension Test was designed based on the psycholinguistic method, and the mental process and the ability of understanding garden path sentences were tested by the agent task experiment paradigm. Finally, the processing strategies and error types of Chinese spoken garden path sentences for HFA children were explored. ResultsThe score in understanding garden path sentences was lower (t = -2.941, P < 0.01), and the reaction time in processing garden path sentences was significantly longer (t = 6.132, P < 0.001) in HFA children than in TD children, however, there was no significant difference in the number of childern mastering garden path sentences between two groups (χ² = 2.954, P > 0.05). In terms of the error types of "animate noun + verb + animate noun + 's + noun" (AVA) and "animate noun + verb + inanimate noun + 's + noun" (AVI) garden path sentences, both groups showed more errors in "pursuit of correct word order" than in "pursuit of semantically correct" (t > 2.503, P < 0.05). In "inanimate noun + verb + animate noun + 's + noun" (IVA) sentences, HFA children showed less erros in "pursuit of correct word order" than in "pursuit of semantically correct" (t = -6.523, P < 0.001), however, no difference was found between them in TD children (t = 2.024, P > 0.05). ConclusionThe processing and comprehension ability of Chinese spoken garden path sentences in HFA children aged five to seven yeas are poor. HFA children are more sensitive to semantics, and garden path sentences with semantic violations are more likely to lead to HFA children's understanding errors. In the garden path sentence patterns of AVA and AVI, HFA children appear similar sentence understanding and processing strategies as TD children. In the garden path of IVA, HFA children use a sentence processing strategy combining word order and semantics, but semantics play a more prominent role, while TD children use a more stable word order processing strategy.

BackgroundAcne treatment cycle lasts long and will cause facial appearance damage. Many patients are prone to psychological problems and severe patients may even experience suicidal ideation.However， the influencing factors of psychological health problems in acne patients are still unclear. ObjectiveTo investigate the mental health status of patients with moderate-to-severe acne and to analyze the influencing factors of their psychological health problems， so as to provide references for improving their mental health. MethodsA total of 120 patients with moderate-to-severe acne of grades II-IV were selected as the research subjects， who were treated in the dermatology outpatient department of the Third Hospital of Mianyang from June 2021 to June 2023. All subjects were evaluated by using the Acne-Specific Quality of Life Questionnaire（Acne-QoL）， Self-rating Depression Scale （SDS）， Self-rating Anxiety Scale （SAS）， Rosenberg Self-Esteem Scale （RSES） and Social Phobia Inventory （SPIN）. According to the evaluation results of SDS， SAS， RSES， SPIN， subjects were divided into a group with mental health problems （n=21） and a group without mental health problems （n=99）. Binary Logistic regression anaylsis was adopted to explore the influencing factors of psychological health problems in acne patients. ResultsA total of 21 patients （17.50%） were detected to have symptoms of depression， anxiety， inferiority or social dysfunction. Statistically significant difference was observed in comparison between two groups in gender， profession， acne classification， dietary habit， age， illness course as well as the factor scores of self perception， emotional function， social function and acne symptom in Acne-QoL （χ²=7.013， 23.123， 9.028， 11.327， t=9.913， 13.022， 4.081， 5.383， 5.361， 10.203，P<0.05）. The results of binary Logistic regression analysis showed that the followings were risk factors for the occurrence of psychological health problems in acne patients： female （OR=2.243， 95% CI： 1.136~4.429）， acne of grade III （OR=3.615， 95% CI： 1.269~10.295） or IV （OR=1.872， 95% CI： 1.073~3.266）， course of disease≥ 1.6 years （OR=2.499， 95% CI：1.068~5.851）， a spicy or greasy diet （OR=3.811， 95% CI： 1.169~12.427）， Acne-QoL self perception score ≤18 （OR=1.802， 95% CI：1.227~2.646）， Acne-QoL emotional function score ≤18 （OR=2.252， 95% CI： 1.016~4.992）， Acne-QoL social function score ≤14 （OR=3.515， 95% CI： 1.534~8.053） and Acne-QoL acne symptom score ≤18 （OR=3.586， 95% CI： 1.098~11.715）. Protective factors for psychological health problems in acne patients included age over 30 years old （OR=0.429， 95% CI： 0.283~0.648）， occupation as professional cadre or enterprise employee （OR=0.483， 95% CI： 0.249~0.939） and other occupations （OR=0.276， 95% CI： 0.090~0.850）. ConclusionA part of patients with moderate-to-severe acne may experience psychological health problems. Patients with female gender， higher acne grades， longer disease duration， preference for greasy or spicy foods and lower scores in all Acne-QoL factors have a higher risk of experiencing psychological health problems.