Because of immature gastrointestinal tract development and the influence of postnatal disease status,extremely low birth weight infant feeding has become a majoy problem. Early positive parenteral feeding and proper enteral feeding are very helpful to the growth and development of extremely low birth weight infant.

OBJECTIVE To evaluate the value of HPV-DNA test in the screening of cerical lesions.METHODS Patients with cervical lesions were detected by HPV-DNA test Papanicolaou(Pap) smear and pathological inspection,and the pathological results were regarded as gold standard.From the 178 positive cases,we compared the sensitivity of HPV-DNA test and Pap smear,and the HPV-DNA detection rate from patients with different histocytological classification.RESULTS All of 178 cases were positive in pathological inspection,the HPV-DNA test sensitivity was 85.4%,whereas Pap smear was 50.6%,the difference between HPV-DNA test and Pap smear was significant(P

By engineering the subsite +1 of cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans, we improved its maltodextrin specificity for 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) synthesis. Specifically, we conducted site-saturation mutagenesis on Leu194, Ala230, and His233 in subsite +1 separately and gained 3 mutants L194N (leucine --> asparagine), A230D (alanine --> aspartic acid), and H233E (histidine --> glutamic acid) produced higher AA-2G yield than the wild-type and the other mutant CGTases. Therefore, the 3 mutants L194N, A230D, and H233E were further used to construct the double and triple mutations. Among the 7 obtained combinational mutants, the triple mutant L194N/A230D/H233E produced the highest AA-2G titer of 1.95 g/L, which was increased by 62.5% compared with that produced by the wild-type CGTase. Then, we modeled the reaction kinetics of all the mutants and found a substrate inhibition by high titer of L-AA for the mutants. The optimal temperature, pH, and reaction time of all the mutants were also determined. The structure modeling indicated that the enhanced maltodextrin specificity may be related with the changes of hydrogen bonding interactions between the side chain of residue at the three positions (194, 230 and 233) and the substrate sugars.
Ascorbic Acid ; analogs & derivatives ; chemistry ; Glucosyltransferases ; genetics ; metabolism ; Hydrogen Bonding ; Kinetics ; Mutagenesis, Site-Directed ; Paenibacillus ; enzymology ; Polysaccharides ; chemistry ; Protein Engineering ; Substrate Specificity ; Temperature

Objective To develop a method for determination of iridoid glycosides in Morinda officinalis How.and optimize the extraction methods for iridoid glycosides in Morinda officinalis How.Methods The iridoid glycosides, including monotropein, deacetyl asperulosidic acid,asperulosidic acid and asperuloside as standards, HPLC method was developed to determine the content of iridoid glycosides in Morinda officinalis How.The separation was performed on Venusil MP C18 (250 mm×4.6 mm, 5 μm) column.The mobile phase was acetonitrile (A)-0.2% phosphoric acid and 0.01 disodium hydrogen phosphate buffer salt (B) with gradient elution (0-12 min, 1%-2% A;12-30 min, 2%-25% A).The detection wavelength was 235 nm.The flow rate was set at 1.0 ml/min and the column temperature at 25 ℃.The injection volume was 20 μl.Single factor analysis and orthogonal test were used to optimize extraction method of iridoid glycosides in Morinda officinalis How.Results Monotropein, deacetyl asperulosidic acid, asperulosidic acid and asperuloside showed good linearity (r>0.999 5) in the ranges of 0.375-12 μg, 0.13-4.16 μg, 0.016-0.516 μg and 0.012-0.384 μg, respectively.This validated method has good repeatability, precision, recovery and stability.It was conformed to meet the requirements and regulation.The optimal extraction method included soaking the raw materials with 16 times of 10% ethanol for 9 h, and then extraction by percolation with the flow rate of 0.8 BV/h.Conclusion The HPLC method sensitively and precisely determined the content of iridoid glycosides in Morinda officinalis How.The optimized extraction method extracted these constituents effectively.

Growth plate,the developmental center of endochondral osteogenesis,can be divided morphologically and functionally into a resting zone,a proliferative zone,a prehypertrophic zone and a hypertrophic zone.Injuries to growth plate often lead to bone growth defects including limb length discrepancy and angulation deformity in children.Currently,their orthopedic corrective surgeries are invasive and limitedly effective and no effective biotherapy has been available.Previous studies on animal models of growth plate damage have investigated the related cellular and molecular events in the repair of damaged growth plates in the 4 distinct inflammatory,fibrogenic,osteogenic and remodeling phases.Related molecules involved in the regulation of the above processes,such as inflammatory cytokines tumor necrosis factor alpha,mitogenic platelet-derived growth factor and bone morphogenetic protein,are found to participate in the regulation of growth plate injury.Exploration of the mechanisms may provide new targets for biotherapy.In addition,development of cartilage tissue engineering,especially application of mesenchymal stem cells,also provides potential interventions for growth plate injury.

Objective To study the correlation between polymorphism of intron 5 gene of surfactant protein B(SP-B)and bronchopulmonary dysplasia(BPD) in neonates and to explore the relevance of the pathogenesis of BPD at the genetic level.Methods Fifty infants with BPD admitted to the NICU in the Hos-pital of Inner Mongolia Medical University from November 2016 to November 2017 were selected as the BPD group.Among them,13 were Mongolian nationality,37 were Han nationality. Fifty newborn without BPD during the same period were assigned to the control group in this study,among them,15 were Mongolian na-tionality,35 were Han nationality.PCR technique was used to detect SP-B gene intron 5 polymorphism and its genotype and allele distribution. Results Regardless of Mongolian or Han nationality,three genotypes of SP-B intron 5 genotypes were detected:wild type,insert type and deletion type.The frequencies of the three genotypes were 73.0%(27/37),10.8%(4/37) and 16.2%(6/37) respectively,and the allele frequencies were 77.0%(57/74),9.5%(7/74) and 13.5%(10/74) respectively in the Han nationality BPD group.The frequencies of the three genotypes in the Han nationality control group were 82.9%(29/35),11.4%(4/35) and 5.7%(2/35)respectively;the allele frequencies were 85.7%(60/70),8.6%(6/70) and 5.7%(4/70) respectively.In the Mongolian BPD group,the frequencies of the above three genotypes were 53.8%(7/13), 15.4%(2/13) and 30.8%(4/13) respectively,and the allele frequencies were 61.5%(16/26),15.4%(4/26) and 23.1%(6/26) respectively.The frequencies of the above three genotypes in the Mongolian con-trol group were 53.3%(8/15),26.7%(4/15) and 20.0%(3/15),the allele frequencies were 66.7%(20/30),20.0%(6/30),13.3%(4/30) respectively.There were no significant differences in the allele fre-quencies and the genotypes of SP-B intron 5 between the Han nationality BPD group and the Han control group,the Mongolian BPD group and the Mongolian control group(all P>0.05).Conclusion No poly-morphisms of SP-B intron 5 and BPD in newborns of the Han nationality and Mongolian nationnality in Inner Mongolia are found.

Objective To study the polymorphism of vascular endothelial growth factor ( VEGF) gene－460T ＞C locus and its correlation with bronchopulmonary dysplasia ( BPD ) in preterm infants. Method From October 2014 to October 2017, a prospective study was conducted on BPD preterm infants admitted to the neonatal department of the Affiliated Hospital of Inner Mongolia Medical University .During the same time, non-BPD preterm infants of the same nation and same ethnic group were selected as the control group.The ratio between the BPD group and the control group was 1∶1.Genotype and allelic distribution of VEGF－460T＞C loci in both groups were detected using polymerase chain reaction method . Result A total of 100 BPD infants were enrolled in the BPD group , including 50 cases of Mongolian nationality and 50 cases of Han nationality.Both TT and CT genotypes were detected in the VEGF －460T＞C locus in the BPD and control groups .The frequencies of the two genotypes were 38.0%and 62.0%in the BPD group, 29.0% and 71.0% in the control group.The C allele frequencies were 31.0% in the BPD group and 35.5%in the control group.The T allele frequencies were 69.0%in the BPD group and 64.5%in the control group.No significant differences existed in allele and genotype frequencies between the two groups (P＝0.178 and 0.338).The frequencies of the two genotypes in the Mongolian BPD infants and the Han BPD infants were 40.0%and 60.0%vs.36.0%and 64.0%.The C allele frequencies were 30.0%and 32.0%, and the T allele frequencies were 70.0% and 68.0%.No significant differences existed in allele and genotype frequencies between the two groups ( P ＝0.680 and 0.648 ) .Conclusion No correlation existed between the polymorphism of VEGF －460T＞C locus and the occurrence and development of BPD in premature infants in Inner Mongolia.

Objective:To investigate the correlation between serum ischemia modified albumin (IMA) and calmodulin (CaM) expression levels and neurological impairment in patients with cerebral small vessel disease.Methods:The clinical data of 140 patients with cerebral small vessel disease (CSVD) who received treatment at The Third People Hospital in Liaocheng between April 2020 and December 2021 were retrospectively analyzed. On admission, serum levels of CaM and IMA were measured using an enzyme-linked immunosorbent assay and an albumin-cobalt binding test. Patients' neurological function was evaluated using the National Institutes of Health Stroke Scale (NIHSS). Patients' transient cerebral ischemia, urinary incontinence, and gait disturbance were evaluated using the National Institute of Neurological Disorders and Stroke Scale. Patients' cognitive function was evaluated using the Montreal Cognitive Assessment scale. The influential factors of serum IMA and CaM expression levels in patients with CSVD were analyzed. The factors that affect the severity of neuological imairment in patients with CSVD and their correlation with serum IMA and CaM expression levels were analyzed.Results:The gender, age, presence or absence of gait disorders, and presence or absence of urinary incontinence of patients were not correlated with serum IMA and CaM levels (all P > 0.05). The serum levels of IMA [(38.5 ± 5.3) × 103U/L, (38.5 ± 4.7) × 103U/L, (39.0 ± 4.4) × 103U/L] and CaM [(190.4 ± 34.5) μg/L, (191.2 ± 26.7) μg/L, (199.7 ± 24.8) μg/L] in patients with cognitive impairment, dizziness and vertigo, and transient cerebral ischemia were significantly higher than those in patients with normal cognitive function, patients without dizziness and vertigo, or patients without transient cerebral ischemia [(27.3 ± 4.4) × 103U/L, (21.0 ± 3.8) × 103U/L, (20.5 ± 5.1) × 103U/L, (180.6 ± 29.6) μg/L, (179.5 ± 28.6) μg/L, (168.6 ± 32.4) μg/L, t = 14.10, 24.36, 22.50, all P < 0.05]. There were significant differences in cognitive impairment (38/16/9), dizziness and vertigo (39/16/8), transient cerebral hemorrhage (35/16/9), NIHSS score [(3.6 ± 0.8) points, (7.5 ± 0.9) points, (16.2 ± 3.2) points], CaM levels [(125.3 ± 20.5) μg/L, (185.5 ± 23.6) μg/L, (237.9 ± 54.3) μg/L], and IMA levels [(21.2 ± 3.5)] × 103 U/L, [(38.5 ± 4.3) × 103 U/L, (74.9 ± 5.4) × 103 U/L] among patients with mild, moderate, and severe neurological impairment ( t = 32.87, 11.28, 12.42, 34.59, 151.73, 147.84, all P < 0.05). The results of multivariate analysis indicated that cognitive impairment ( OR = 1.578, 95% CI: 1.043-2.386), transient cerebral ischemia ( OR = 2.396, 95% CI: 1.156-4.969), dizziness and vertigo ( OR = 1.906, 95% CI: 1.086-3.345), NIHSS score ( OR = 2.171, 95% CI: 1.162-4.056), CaM level ( OR = 2.022, 95% CI: 1.268-3.224), and increased IMA levels ( OR = 2.090, 95% CI: 1.313-3.325) were independent influential factors for worsened neurological impairment (all P < 0.05). The serum levels of IMA and CaM in patients with CSVD were significantly positively correlated with the severity of neurological impairment ( r = 5.45, 8.33, both P < 0.05). Conclusion:The elevated serum levels of IMA and CaM in patients with CSVD serve as independent risk factors for neurological impairment, and these levels are positively correlated with the severity of neurological impairment.

Objective To evaluate the effect between two different methods in hemostasis by compression after the radial artery puncture .Methods Six clinical studies in the domestic databases were selected according to the inclusion standards .1 017 patients used either the artery compressor or elastic bandage .The fixed effect model was used in the Meta analysis .Results In 6 clinical studies , the effect of radial artery compressor in reducing subcutaneous blood infiltration and hematoma were significant (OR=0.30, 95%CI 0.08 ~0.43,P<0.05).In 6 controlled clinical studies, the effect of radial artery compressor in reducing the rate of postoperative limbs swelling was significant (OR=0.14,95%CI 0.10~0.19,P<0.05). Conclusions Radial artery compressor has great effects in reducing postoperative complications , compared with traditional elastic bandage , especially in reducing subcutaneous blood infiltration , hematoma and prevention of postoperative limbs swelling .

Objective:To explore the relationship between rs2010963, rs3025039 and rs699947 gene polymorphism of vascular endothelial growth factor(VEGF) gene and bronchopulmonary dysplasia(BPD) in Mongolian premature infants.Methods:A case-control design was used to collect 50 cases of Mongolian premature infants who were hospitalized at the Affiliated Hospital of Inner Mongolia Medical University and diagnosed with BPD from January 2016 to December 2020 as the observation group, while 56 cases of non-BPD premature infants of the same nationality and time period were selected as the control group.Using PCR method to detect the genotype and allele distribution of the VEGF gene rs2010963, rs3025039 and rs699947 locus.Combining clinical data to analyze whether the above gene loci were related to the onset of premature infants with Mongolian BPD in our area.Results:Through genetic testing, it was found that CC, CA and AA genotypes can be detected at the rs699947 site of VEGF gene in premature infants in both the observation group and the control group.The frequencies of the three genotypes in the observation group were 16.0%, 24.0%, and 60.0%, respectively; the frequency of the C allele was 28.0%, the frequency of the A allele was 72.0%, and the frequency of the three genotypes in the control group was 32.1.%, 32.1% and 35.7%, respectively.The frequency of C allele was 48.2%, the frequency of G allele was 51.8%, and the allele and genotype frequencies of this locus between the observation group and the control group were significant differences from those of the control group( P<0.05). Conclusion:The polymorphism of VEGF gene rs699947 locus is associated with the occurrence and development of BPD in Mongolian premature infants, and allele A may be a susceptible factor.