Because of immature gastrointestinal tract development and the influence of postnatal disease status,extremely low birth weight infant feeding has become a majoy problem. Early positive parenteral feeding and proper enteral feeding are very helpful to the growth and development of extremely low birth weight infant.

OBJECTIVE To evaluate the value of HPV-DNA test in the screening of cerical lesions.METHODS Patients with cervical lesions were detected by HPV-DNA test Papanicolaou(Pap) smear and pathological inspection,and the pathological results were regarded as gold standard.From the 178 positive cases,we compared the sensitivity of HPV-DNA test and Pap smear,and the HPV-DNA detection rate from patients with different histocytological classification.RESULTS All of 178 cases were positive in pathological inspection,the HPV-DNA test sensitivity was 85.4%,whereas Pap smear was 50.6%,the difference between HPV-DNA test and Pap smear was significant(P

By engineering the subsite +1 of cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans, we improved its maltodextrin specificity for 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) synthesis. Specifically, we conducted site-saturation mutagenesis on Leu194, Ala230, and His233 in subsite +1 separately and gained 3 mutants L194N (leucine --> asparagine), A230D (alanine --> aspartic acid), and H233E (histidine --> glutamic acid) produced higher AA-2G yield than the wild-type and the other mutant CGTases. Therefore, the 3 mutants L194N, A230D, and H233E were further used to construct the double and triple mutations. Among the 7 obtained combinational mutants, the triple mutant L194N/A230D/H233E produced the highest AA-2G titer of 1.95 g/L, which was increased by 62.5% compared with that produced by the wild-type CGTase. Then, we modeled the reaction kinetics of all the mutants and found a substrate inhibition by high titer of L-AA for the mutants. The optimal temperature, pH, and reaction time of all the mutants were also determined. The structure modeling indicated that the enhanced maltodextrin specificity may be related with the changes of hydrogen bonding interactions between the side chain of residue at the three positions (194, 230 and 233) and the substrate sugars.
Ascorbic Acid ; analogs & derivatives ; chemistry ; Glucosyltransferases ; genetics ; metabolism ; Hydrogen Bonding ; Kinetics ; Mutagenesis, Site-Directed ; Paenibacillus ; enzymology ; Polysaccharides ; chemistry ; Protein Engineering ; Substrate Specificity ; Temperature

Objective To develop a method for determination of iridoid glycosides in Morinda officinalis How.and optimize the extraction methods for iridoid glycosides in Morinda officinalis How.Methods The iridoid glycosides, including monotropein, deacetyl asperulosidic acid,asperulosidic acid and asperuloside as standards, HPLC method was developed to determine the content of iridoid glycosides in Morinda officinalis How.The separation was performed on Venusil MP C18 (250 mm×4.6 mm, 5 μm) column.The mobile phase was acetonitrile (A)-0.2% phosphoric acid and 0.01 disodium hydrogen phosphate buffer salt (B) with gradient elution (0-12 min, 1%-2% A;12-30 min, 2%-25% A).The detection wavelength was 235 nm.The flow rate was set at 1.0 ml/min and the column temperature at 25 ℃.The injection volume was 20 μl.Single factor analysis and orthogonal test were used to optimize extraction method of iridoid glycosides in Morinda officinalis How.Results Monotropein, deacetyl asperulosidic acid, asperulosidic acid and asperuloside showed good linearity (r>0.999 5) in the ranges of 0.375-12 μg, 0.13-4.16 μg, 0.016-0.516 μg and 0.012-0.384 μg, respectively.This validated method has good repeatability, precision, recovery and stability.It was conformed to meet the requirements and regulation.The optimal extraction method included soaking the raw materials with 16 times of 10% ethanol for 9 h, and then extraction by percolation with the flow rate of 0.8 BV/h.Conclusion The HPLC method sensitively and precisely determined the content of iridoid glycosides in Morinda officinalis How.The optimized extraction method extracted these constituents effectively.

Objective To study the correlation between polymorphism of intron 5 gene of surfactant protein B(SP-B)and bronchopulmonary dysplasia(BPD) in neonates and to explore the relevance of the pathogenesis of BPD at the genetic level.Methods Fifty infants with BPD admitted to the NICU in the Hos-pital of Inner Mongolia Medical University from November 2016 to November 2017 were selected as the BPD group.Among them,13 were Mongolian nationality,37 were Han nationality. Fifty newborn without BPD during the same period were assigned to the control group in this study,among them,15 were Mongolian na-tionality,35 were Han nationality.PCR technique was used to detect SP-B gene intron 5 polymorphism and its genotype and allele distribution. Results Regardless of Mongolian or Han nationality,three genotypes of SP-B intron 5 genotypes were detected:wild type,insert type and deletion type.The frequencies of the three genotypes were 73.0%(27/37),10.8%(4/37) and 16.2%(6/37) respectively,and the allele frequencies were 77.0%(57/74),9.5%(7/74) and 13.5%(10/74) respectively in the Han nationality BPD group.The frequencies of the three genotypes in the Han nationality control group were 82.9%(29/35),11.4%(4/35) and 5.7%(2/35)respectively;the allele frequencies were 85.7%(60/70),8.6%(6/70) and 5.7%(4/70) respectively.In the Mongolian BPD group,the frequencies of the above three genotypes were 53.8%(7/13), 15.4%(2/13) and 30.8%(4/13) respectively,and the allele frequencies were 61.5%(16/26),15.4%(4/26) and 23.1%(6/26) respectively.The frequencies of the above three genotypes in the Mongolian con-trol group were 53.3%(8/15),26.7%(4/15) and 20.0%(3/15),the allele frequencies were 66.7%(20/30),20.0%(6/30),13.3%(4/30) respectively.There were no significant differences in the allele fre-quencies and the genotypes of SP-B intron 5 between the Han nationality BPD group and the Han control group,the Mongolian BPD group and the Mongolian control group(all P>0.05).Conclusion No poly-morphisms of SP-B intron 5 and BPD in newborns of the Han nationality and Mongolian nationnality in Inner Mongolia are found.

Objective To study the polymorphism of vascular endothelial growth factor ( VEGF) gene－460T ＞C locus and its correlation with bronchopulmonary dysplasia ( BPD ) in preterm infants. Method From October 2014 to October 2017, a prospective study was conducted on BPD preterm infants admitted to the neonatal department of the Affiliated Hospital of Inner Mongolia Medical University .During the same time, non-BPD preterm infants of the same nation and same ethnic group were selected as the control group.The ratio between the BPD group and the control group was 1∶1.Genotype and allelic distribution of VEGF－460T＞C loci in both groups were detected using polymerase chain reaction method . Result A total of 100 BPD infants were enrolled in the BPD group , including 50 cases of Mongolian nationality and 50 cases of Han nationality.Both TT and CT genotypes were detected in the VEGF －460T＞C locus in the BPD and control groups .The frequencies of the two genotypes were 38.0%and 62.0%in the BPD group, 29.0% and 71.0% in the control group.The C allele frequencies were 31.0% in the BPD group and 35.5%in the control group.The T allele frequencies were 69.0%in the BPD group and 64.5%in the control group.No significant differences existed in allele and genotype frequencies between the two groups (P＝0.178 and 0.338).The frequencies of the two genotypes in the Mongolian BPD infants and the Han BPD infants were 40.0%and 60.0%vs.36.0%and 64.0%.The C allele frequencies were 30.0%and 32.0%, and the T allele frequencies were 70.0% and 68.0%.No significant differences existed in allele and genotype frequencies between the two groups ( P ＝0.680 and 0.648 ) .Conclusion No correlation existed between the polymorphism of VEGF －460T＞C locus and the occurrence and development of BPD in premature infants in Inner Mongolia.

Growth plate,the developmental center of endochondral osteogenesis,can be divided morphologically and functionally into a resting zone,a proliferative zone,a prehypertrophic zone and a hypertrophic zone.Injuries to growth plate often lead to bone growth defects including limb length discrepancy and angulation deformity in children.Currently,their orthopedic corrective surgeries are invasive and limitedly effective and no effective biotherapy has been available.Previous studies on animal models of growth plate damage have investigated the related cellular and molecular events in the repair of damaged growth plates in the 4 distinct inflammatory,fibrogenic,osteogenic and remodeling phases.Related molecules involved in the regulation of the above processes,such as inflammatory cytokines tumor necrosis factor alpha,mitogenic platelet-derived growth factor and bone morphogenetic protein,are found to participate in the regulation of growth plate injury.Exploration of the mechanisms may provide new targets for biotherapy.In addition,development of cartilage tissue engineering,especially application of mesenchymal stem cells,also provides potential interventions for growth plate injury.

Objective:To investigate the correlation between serum ischemia modified albumin (IMA) and calmodulin (CaM) expression levels and neurological impairment in patients with cerebral small vessel disease.Methods:The clinical data of 140 patients with cerebral small vessel disease (CSVD) who received treatment at The Third People Hospital in Liaocheng between April 2020 and December 2021 were retrospectively analyzed. On admission, serum levels of CaM and IMA were measured using an enzyme-linked immunosorbent assay and an albumin-cobalt binding test. Patients' neurological function was evaluated using the National Institutes of Health Stroke Scale (NIHSS). Patients' transient cerebral ischemia, urinary incontinence, and gait disturbance were evaluated using the National Institute of Neurological Disorders and Stroke Scale. Patients' cognitive function was evaluated using the Montreal Cognitive Assessment scale. The influential factors of serum IMA and CaM expression levels in patients with CSVD were analyzed. The factors that affect the severity of neuological imairment in patients with CSVD and their correlation with serum IMA and CaM expression levels were analyzed.Results:The gender, age, presence or absence of gait disorders, and presence or absence of urinary incontinence of patients were not correlated with serum IMA and CaM levels (all P > 0.05). The serum levels of IMA [(38.5 ± 5.3) × 103U/L, (38.5 ± 4.7) × 103U/L, (39.0 ± 4.4) × 103U/L] and CaM [(190.4 ± 34.5) μg/L, (191.2 ± 26.7) μg/L, (199.7 ± 24.8) μg/L] in patients with cognitive impairment, dizziness and vertigo, and transient cerebral ischemia were significantly higher than those in patients with normal cognitive function, patients without dizziness and vertigo, or patients without transient cerebral ischemia [(27.3 ± 4.4) × 103U/L, (21.0 ± 3.8) × 103U/L, (20.5 ± 5.1) × 103U/L, (180.6 ± 29.6) μg/L, (179.5 ± 28.6) μg/L, (168.6 ± 32.4) μg/L, t = 14.10, 24.36, 22.50, all P < 0.05]. There were significant differences in cognitive impairment (38/16/9), dizziness and vertigo (39/16/8), transient cerebral hemorrhage (35/16/9), NIHSS score [(3.6 ± 0.8) points, (7.5 ± 0.9) points, (16.2 ± 3.2) points], CaM levels [(125.3 ± 20.5) μg/L, (185.5 ± 23.6) μg/L, (237.9 ± 54.3) μg/L], and IMA levels [(21.2 ± 3.5)] × 103 U/L, [(38.5 ± 4.3) × 103 U/L, (74.9 ± 5.4) × 103 U/L] among patients with mild, moderate, and severe neurological impairment ( t = 32.87, 11.28, 12.42, 34.59, 151.73, 147.84, all P < 0.05). The results of multivariate analysis indicated that cognitive impairment ( OR = 1.578, 95% CI: 1.043-2.386), transient cerebral ischemia ( OR = 2.396, 95% CI: 1.156-4.969), dizziness and vertigo ( OR = 1.906, 95% CI: 1.086-3.345), NIHSS score ( OR = 2.171, 95% CI: 1.162-4.056), CaM level ( OR = 2.022, 95% CI: 1.268-3.224), and increased IMA levels ( OR = 2.090, 95% CI: 1.313-3.325) were independent influential factors for worsened neurological impairment (all P < 0.05). The serum levels of IMA and CaM in patients with CSVD were significantly positively correlated with the severity of neurological impairment ( r = 5.45, 8.33, both P < 0.05). Conclusion:The elevated serum levels of IMA and CaM in patients with CSVD serve as independent risk factors for neurological impairment, and these levels are positively correlated with the severity of neurological impairment.

Objective To study the relationship between exon10 region mutation of adenosine triphosphate-binding cassette transporters A3 (ABCA3) gene and neonatal respiratory distress syndrome (RDS).Method From September 2014 to January 2018,neonates admitted to the neonatal intensive care unit of our hospital were studied.Mongolian and Han Chinese newborns with RDS were assigned into Mongolian RDS group and Han RDS group.Mongolian and Han Chinese newborns without RDS were assigned into Mongolian control group and Han control group.The genotype frequencies and allele frequencies of ABCA3 exon10 in each group were compared.Result A total of 320 cases were studied,including 60 cases in Mongolian RDS group,100 cases in Han RDS group,60 cases in Mongolian control group,and 100 cases in Han control group.A single-base point mutation C ＞ T in the ABCA3 rs13332514 (F353F) locus was founded in both Mongolian and Han Chinese newborns.The mutation rates in Mongolian RDS group and Mongolian control group were 26.7％ and 18.3％,and the difference was statistically significant (x2 =6.316,P =0.043);the mutation rates in Han RDS group and Han control group were 21.0％ and 13.0％,the difference was also statistically significant (x2 =7.426,P =0.009).No significant differences existed between Mongolian RDS group and Han RDS group,and between Mongolian control group and Han control group (P ＞ 0.05).The genotypes of CC,CT and TT were detected in both Mongolian and Han RDS groups,and the CC and CT genotypes were also detected in the control group.The T allele frequency was 18.3％ in Mongolian RDS group,and 9.2％ in Mongolian control group.The difference was statistically significant (x2 =4.251,P =0.039).The T allele frequency in Han RDS group was 15.0％,and 6.5％ in Han control group.The difference was also statistically significant (x2 =7.530,P =0.006).Conclusion A single-base point mutation C ＞T in the rs13332514 (F353F) locus of ABCA3 exonl0 may be related to the occurrence of RDS in Mongolian and Han newborns in Inner Mongolia.Allele T may be one of the susceptibility genes of RDS.

Objective:To explore the carrier status of group B streptococci (GBS) in pregnant women of Mongolian and Han nationality and the neonatal GBS infection in order to identify the high risk factors of GBS infection in Mongolian and Han newborns in this area.Methods:Totally 7289 pregnant women and their newborns born alive were tested for GBS in the Affiliated Hospital of Inner Mongolia Medical University from June 2017 to June 2020, and their newborns were cultured for GBS, and the venous blood of newborns delivered by GBS positive women were detected for anti-GBS capsular polysaccharide antibody level, in order to determine the high risk factors of neonatal GBS infection.Results:Among the 7289 pregnant women, 3136 were Mongolian pregnant women (2599 full-term delivery and 537 premature delivery) and 4153 were Han pregnant women (3541 full-term delivery and 612 premature delivery). The results of GBS test showed that the GBS carrier rate was 8.19% in the Mongolian preterm delivery group, 4.35% in the Mongolian term group, 11.93% in the Han preterm group, and 5.76% in the Han term group, indicating that the carrier rate of GBS in the preterm group was significantly higher than that in the term group, regardless of Mongolian and Han nationality ( P < 0.05). Further comparing the GBS carrier rate of Mongolian and Han pregnant women, the GBS carrier rate of Mongolian pregnant women was significantly lower than that of Han pregnant women regardless of the premature delivery group and term group ( P < 0.05). (2) A total of 434 newborns were born by GBS positive parturients. The positive rates of GBS in Mongolian premature infants, Mongolian full-term infants, Han premature infants and Han full-term infants were 29.55%, 14.16%, 31.51% and 17.65%, respectively, suggesting that the positive rate of GBS in premature infants was significantly higher than that in full-term infants, regardless of Mongolian and Han nationality ( P<0.05). Further comparing the positive rate of GBS in Mongolian and Han newborns, there was no significant difference in the positive rate of GBS between Mongolian newborns and Han newborns, no matter the premature delivery group and the full-term group. (3) This study compared the incidence of early-onset GBS septicemia in Mongolian and Han newborns. The results showed that the incidence of early-onset GBS septicemia in Mongolian premature infants was 23.08%, and none in full-term infants. The incidence of early-onset GBS septicemia in Han premature infants was 26.09%. The incidence of early-onset GBS septicemia in term infants was 5.56%. The incidence of neonatal GBS septicemia in the preterm group was significantly higher than that in the term group, regardless of Mongolian and Han nationality. By further comparing the incidence of GBS septicemia in Mongolian and Han newborns, there was no significant difference in the positive rate of GBS between Mongolian newborns and Han newborns regardless of the premature delivery group and the term group. (4) In both Mongolian and Han nationality, the level of anti-GBS capsular polysaccharide antibody in premature infants was significantly lower than that in term infants ( P < 0.05). (5) Regardless of the Mongolian and Han nationality, compared with GBS negative group, GBS positive rate was higher in pregnant women aged≥35 years old, with history of menstruation, miscarriage, vaginitis, floating population, and those who had not undergone pre-pregnancy examination,,which were the high risk factors for GBS-positive pregnant women during pregnancy. (6) In both Mongolian and Han nationality, the incidence of chorioamnionitis, puerperal infection, premature delivery and premature rupture of membranes in the GBS positive group was higher than that in the GBS negative group, and the incidence of fetal distress and neonatal asphyxia in the GBS positive group was also higher than that in the GBS negative group. Conclusions:The carrier rate of GBS in Mongolian pregnant women is lower than that in Han pregnant women, and positive GBS during pregnancy will increase the incidence of adverse maternal and fetal outcomes such as chorioamnionitis, puerperal infection, premature delivery, premature rupture of membranes, fetal distress, neonatal asphyxia and neonatal early-onset GBS septicemia. The high risk factors are maternal age ≥ 35 years old, history of menstruation, abortion, vaginitis, floating population, and infection without pre-pregnancy examination. We should attach great importance to the perinatal high risk factors and formulate corresponding intervention measures accordingly, and make rational use of antibiotics for prenatal prevention, so as to further reduce the incidence of early-onset GBS septicemia in newborns.