OBJECTIVE:To explore the application situation of antibacterials in surgical departments of a hospital so as to provide epidemiological evidence for further standardized administration of antibiotics. METHODS:900 medical records during 2005～2007 were randomly collected from surgical departments for meta-analysis with regard to the rate of patients receiving antibacterials,rationality and the cause of irrational use of antibiotics,etc. RESULTS:97.4% of the patients received antibiotics,and the rate of preventive medication which accounted for 82.8% showed an upward trend year by year. The irrationality was more serious in preventive medication than in treatment medication,with the rate of irrational use being 98.9%. CONCLUSION:Irrational drug use in surgical departments of this hospital is widespread and serious,and there are many problems needed to be solved urgently.

OBJECTIVETo study the genetic diversity of Changium smyrnioides and give a reference for utilization of the germplasm.

METHODTen different populations of Ch. smyrnioides were analyzed by the approach of sequence-related amplified polymorphism (SRAP). Genetic similarity coefficient was calculated, and systematic relationships were constructed based on the UPGMA method.

RESULTSeventeen primer pairs were selected from 160. A total of 363 bands were scored, 314 bands of them were polymorphic and the average was 18.47 polymorphic bands per primer pair, which were up to 86.50% polymorphic ratio. The results indicated that there was abundant genetic diversity among the tested materials. Genetic similarity coefficient was ranged from 0.4959 to 0.8182. Cluster analysis showed that ten different populations of Ch. smyrnioides could be distinguished into two groups.

CONCLUSIONHigh level genetic diversity was in different populations of Ch. smyrnioides, and genetic relationship was correlative to geographic position.

Apiaceae ; classification ; genetics ; Genetic Variation ; genetics ; Nucleic Acid Amplification Techniques ; methods ; Phylogeny ; Plants, Medicinal ; classification ; genetics ; Polymerase Chain Reaction

OBJECTIVETo observe the pollen morphological differences among different populations of Changium smyrnioides.

METHODThe pollen morphology of 10 populations were examined through LM and SEM observations.

RESULTPollens in different populations were distinguished from each other in the size, the largest average size was the pollen of the population cultivated in Hongshan, and the smallest was that of the population cultivated in Jiuhuashan. Pollens were oval-shaped in all of the populations, and P/E values were around 1.5. Typical feature of surface ornamentation was stripe-like structure, different populations were distinguished from each other in the texture depth and the gap. With different length and width in different populations, typical feature of germinal aperture was nearly square and 3 germinal furrows. Variation with 4 germinal apertures were found in the pollen of population cultivated in Hongshan.

CONCLUSIONDiversity of pollen morphology was high, and differentiation was strong in Ch. smyrnioides.

Apiaceae ; chemistry ; Cevanes ; administration & dosage ; metabolism ; Glycoproteins ; Plant Proteins ; Plant Roots ; chemistry ; Pollen ; growth & development

OBJECTIVETo analyze the residuals of organochlorine pesticides and heavy metals in soil, water and Radix Liriopes from planting bases in Quanzhou city, and evaluate the quality of the herb.

METHODGC method was applied to determine residuals of organochlorine pesticides, the contents of Pb, Cd, Cu, Cr, and As were determined by IPC.

RESULTThe contents of residuals of organochlorine pesticides and heavy metals varied among the samples of soil, water and Radix liriopes, but all of the residuals met requirement of the national standard.

CONCLUSIONResiduals of organochlorine pesticides and heavy metals in soil, water and Radix liriopes from planting bases in Quanzhou city were conformed to GAP.

China ; Hydrocarbons, Chlorinated ; analysis ; Liriope Plant ; chemistry ; Metals, Heavy ; analysis ; Pesticide Residues ; analysis ; blood ; Soil Pollutants ; analysis ; Water Pollutants, Chemical ; analysis

OBJECTIVETo investigate the appearance and moisture, ash content, extract, volatile oil of Panax ginseng and its processed products from different producing areas of northeast China, and thus provide the useful reference data for its quality standard establishment and standardized cultivation.

METHODBase on the Chinese Pharmacopoeia and literatures relating to P. ginseng, the content of moisture, ash, extract, volatile oil in P. ginseng and its processed products from northeast 10 different producing areas were measured, and data analysis of these various indicators was carried out by DTOPSIS.

RESULTThe samples from Changbai, Jian Kangmei and Fusong reached the standard of Chinese pharmacopoeia and national standard, and in addition comprehensive evaluation indicators of them were higher than others samples.

CONCLUSIONThe quality of ginseng from different producing areas appears very different. Some samples failed to reach the standard of Chinese pharmacopoeia and national standard. Ginseng samples from those 3 producing areas of GAP planting bases showed good quality, which reflects the importance of GAP for ginseng cultivation.

China ; Drugs, Chinese Herbal ; chemistry ; Oils, Volatile ; chemistry ; Panax ; chemistry ; growth & development ; Plant Roots ; chemistry ; growth & development

OBJECTIVETo study the effects of salt stress on the growth and resistant-oxidation enzyme activities of Pogostemon cablin.

METHODCutting-rooted seedlings were cultivated in the plastic flowerpot irrigated with various concentrations of NaCl (Hoagland solution) for different duration, and the height, fresh/dry weight, root vitality, SOD,POD and CAT activity, membrane permeability and MDA contents were determined.

RESULTThe growth and development of P. cablin was restrained after salt stress. The differences of salt stress of lower concentration and for a short time (less than 20 mmol x L(-1) and the 9th day) were not obvious. The fresh/ dry weight, growth rate and increasing rate of dry matter were obviously decreased with the increasing of NaCl concentration and prolonging of the time, but membrane permeability and contents of MDA were increased all along, and lipid peroxidation enhanced, while the root vitality and activities of SOD, POD and CAT were firstly increasing at lower concentration and then decreasing at higher concentration.

CONCLUSIONThe growth of P. cablin depends on NaCl concentration and the treated duration.

Antioxidants ; metabolism ; Biomass ; Cell Membrane Permeability ; Lamiaceae ; chemistry ; growth & development ; metabolism ; Lipid Peroxidation ; Seedlings ; chemistry ; growth & development ; metabolism ; Sodium Chloride ; metabolism

OBJECTIVEPrunalla vulgaris was used as the experimental material to study the effects of water stress on the related quality charaters of spadix in P. vulgaris.

METHODBy weighting method to experiment the relative characteristics in vegetative period and reproductive period respectively were studied under timing and quantitative water stress conditions everyday with the method of statistics to compared, including biological characteristics and content of ursolic acid and oleanolic acid.

RESULT AND CONCLUSIONIt is true that the better water range of growth in vegetative period of P. vulgaris is 80% -85%; The 65%-70% field leakage coefficient maximum was the best and the proper water range which would promote the growth, but the best range of active component is 80% -85%.

Oleanolic Acid ; analysis ; Prunella ; chemistry ; growth & development ; metabolism ; Triterpenes ; analysis ; Water ; metabolism

OBJECTIVETo investigate the dynamic changes of main physiological indexes, active constituent of Chrysanthemum morifolium and their correlation during the whole growing period and provided a reference basis for the field production.

METHODTake Ch. morifolium cv. Xiaobaiju, Ch. morifolium cv. Hongxinju and Ch. morifolium cv. Changbanju were taken as the research objects, the main physiological and biochemical indexes were determined, the correlation among them was analyzed.

RESULTThe trend of each main physiological index and active component were different during the whole growing period. Agronomic characters, physiological and biochemical indexes and economic characters had correlation in different degree.

CONCLUSIONVarieties with higher plant height, more branch and cephaloid numbers may be used for breeding for high yield, and varieties with higher chlorophyll content and POD activity, lower GSH content and SOD activity may be used for breeding for high content of the active components.

Breeding ; Chlorophyll ; analysis ; Chrysanthemum ; anatomy & histology ; chemistry ; genetics ; physiology ; Medicine, Chinese Traditional ; Seasons

OBJECTIVECurrent study was conducted to investigate the seed physiological characteristics of Tulipa edulis and improve germination rate.

METHODAnatomical characteristics was observed. Seed water absorption curve was tested by soaking method. Dynamic of embryo development and germination rate as well as germination index under different conditions were recorded. And the biological test of cabbage seed was used for detecting the germination inhibitors.

RESULTThe embryo rate of newly matured seeds was about 10%, and there was no obstacle of water absorption on testa of T. edulis. The optimum method for embryo development was exposure to 300 mg x L(-1) gibberellin solution for 24 hours, and stratification at 25 degrees C for 70 days followed by stratification at 5 degrees C for 40 days. The germintion rate and germination index of dormancy-broken seeds under the dark environment at 10 degrees C and 15 degrees C were significantly higher than those under other conditions. Additionally, there were some germination inhibitory substances in dry seeds.

CONCLUSIONThe seed of T. edulis can be classified as having complex morphophysiological dormancy, and the morphological embryo dormancy played a leading role. Warm and cold stratification resulted in a fast dormancy breaking effect, and a high germination rate more than 90% could be obtained under the optimum conditions.

Germination ; physiology ; Seeds ; physiology ; Tulipa ; physiology

OBJECTIVEPresent study was conducted to explore the growth and photosynthetic characteristics of Tulipa edulis under different light conditions (23%, 45%, 63%, 78%, 100% of full sunlight) and to determine the optimum light intensity for growth of T. edulis.

METHODThe leaf area and biomass indicators as well as reproductive characteristics were measured. The photosynthetic basic parameters and light response curve were determined by a LI-6400XT portable photosynthesis system, and the light response curve characteristic parameters was determined. Additionally, chlorophyll fluorescence parameters were determined by assorted fluorescence leaf chamber of LI-6400XT.

RESULTThe lowest biomass yield was observed in the 23% and 100% of full sunlight treatments while the highest value was found under the 78% of full sunlight conditions. With the reduction of light availability, the success rate of sexual reproduction, light saturation point (LSP) and light compensation point (LCP) reduced, while apparent quantum yield (AQY) increased. 23% and 45% of full sunlight treatments led to lower photosynthesis rate (Pn) and higher apparent quantum yield (AQY) in comparison with other treatents. The highest photosynthesis rate was observed in the 78% and 100% of full sunlight treatments. In addition, 78% of full sunlight treatments led to highest Fv/Fm, Fv'/Fm', PhiPS II, ETR, and qP.

CONCLUSIONT. edulis was able to adapt in a wide range of light intensity, and 78% of full sunlinght was the most suitable light condition for growth of T. edulis.

Biomass ; Dose-Response Relationship, Radiation ; Photosynthesis ; radiation effects ; Sunlight ; Tulipa ; growth & development ; metabolism ; radiation effects