Objective:To investigate the clinical value and age stratification significance of anti-mullerian hormone (AMH) combined with sex hormone in the diagnosis of patients with polycystic ovary syndrome (PCOS).Methods:The clinical data of 164 patients with PCOS(PCOS group) and 120 healthy women(control group) were retrospectively chosen from January 2015 to December 2018.The age, sex hormone index levels and AMH levels of the two groups were compared.The clinical efficacy of single index and combined index detection in the diagnosis of PCOS were analyzed.The correlation between AMH level and age, sex hormone index was evaluated and the influence of age stratification on the diagnosis of PCOS was observed.Results:The levels of AMH, T, LH and LH/FSH ratio in the PCOS group were (62.90±5.19)pmol/L, (1.36±0.55)nmol/L, (12.86±2.4)IU/L, (2.27±0.86), respectively, which were significantly higher than those in the control group [(27.22±3.72)pmol/L, (0.87±0.30)nmol/L, (6.41±1.18)IU/L, (1.15±0.53)] ( t=7.38, 3.76, 5.15, 3.89, all P<0.05). The sensitivity of AMH in the diagnosis of PCOS was significantly higher than LH and LH/FSH(χ 2=5.72, 14.10, all P<0.05). The sensitivity of AMH+ T, AMH+ LH and AMH+ T+ LH were 89.02%, 87.80%, 95.12%, respectively, which were significantly higher than AMH alone (80.49%)(χ 2=5.67, 4.33, 10.94, all P<0.05). There was no significant ststistically difference among AMH, T, LH and LH/FSH in the diagnosis of PCOS(χ 2=0.38, P>0.05). There was no significant ststistically difference in the specificity of AMH alone, AMH+ T, AMH+ LH, AMH+ LH/FSH and combination of AMH+ T+ LH in the diagnosis of PCOS(χ 2=0.45, P>0.05). The AUC of AMH in diagnosis of PCOS was 0.86, which was significantly higher than T, LH and LH/FSH(0.714, 0.765, 0.783)( Z=2.09, 2.43, 2.20, all P<0.05). Spearman correlation analysis showed that there was positive correlation between AMH level and T, LH, LH/FSH in PCOS group ( r=0.27, 0.25, 0.33, all P<0.05). There was negative correlation between AMH level and age in healthy group( P>0.05). The critical value of AMH in diagnosis of PCOS was decreased with the increase of age.The sensitivity, specificity and ROC-AUC of AMH in 25-29 years old group and 30-37 years old group were higher than whole population( r=-0.33, P<0.05). Conclusion:The combination of AMH+ T, AMH+ LH or AMH+ T+ LH can improve the diagnosis sensitivity of PCOS, but the specificity is similar to AMH alone; then age stratification for the AMH critical value can increase the diagnosis accuracy of PCOS.