1.Comparative Study on the Standard Decoction of Yinqiao Powder
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(04):-
Objective To compare the four kinds of Yinqiao Powder prepared by archaic decoction,Japan standard decoction,medical institution decoction and decoction combined archaic with modern technology,select the best method and provide the important theory basis for standardization of decoction technics of diaphoretic recipes.Methods The major contents of traditional Chinese medicine were determined by HPLC and used as the evaluating indicator as well as the ratio of dry extraction.Results The method of archaic decoction got the highest synthetic scores,and the validating experimental result was stable.Conclusion The preparation method of archaic decoction could be used as the reference standard for preparation technology of Yinqiao Powder,in order to further standardize the preparation of the standard decoction of diaphoretic recipes.
2.Neuron stem cell NLRP6 sustains hippocampal neurogenesis to resist stress-induced depression.
Chuanfeng TANG ; Qiaona WANG ; Jingyan SHEN ; Congying WANG ; Hong DING ; Shiyu WEN ; Fan YANG ; Ruiqing JIAO ; Xingxin WU ; Jianmei LI ; Lingdong KONG
Acta Pharmaceutica Sinica B 2023;13(5):2017-2038
Neurogenesis decline in hippocampal dentate gyrus (DG) participates in stress-induced depressive-like behaviors, but the underlying mechanism remains poorly understood. Here, we observed low-expression of NOD-like receptor family pyrin domain containing 6 (NLRP6) in hippocampus of stress-stimulated mice, being consistent with high corticosterone level. NLRP6 was found to be abundantly expressed in neural stem cells (NSCs) of DG. Both Nlrp6 knockout (Nlrp6-/-) and NSC-conditional Nlrp6 knockout (Nlrp6CKO) mice were susceptible to stress, being more likely to develop depressive-like behaviors. Interestingly, NLRP6 was required for NSC proliferation in sustaining hippocampal neurogenesis and reinforcing stress resilience during growing up. Nlrp6 deficiency promoted esophageal cancer-related gene 4 (ECRG4) expression and caused mitochondrial dysfunction. Corticosterone as a stress factor significantly down-regulated NLRP6 expression, damaged mitochondrial function and suppressed cell proliferation in NSCs, which were blocked by Nlrp6 overexpression. ECRG4 knockdown reversed corticosterone-induced NSC mitochondrial function and cell proliferation disorders. Pioglitazone, a well-known clinical drug, up-regulated NLRP6 expression to inhibit ECRG4 expression in its protection against corticosterone-induced NSC mitochondrial dysfunction and proliferation restriction. In conclusion, this study demonstrates that NLRP6 is essential to maintain mitochondrial homeostasis and proliferation in NSCs, and identifies NLRP6 as a promising therapeutic target for hippocampal neurogenesis decline linked to depression.