1.Construction and expression of hTNF-alpha fusion protein mediated by MMP1.
Qiaojiajie ZHAO ; Gan HOU ; Dinan HUANG ; Shuyong CHEN
Journal of Biomedical Engineering 2011;28(3):534-537
This paper is aimed to present a research on fusion protein of human tumor necrosis factor-alpha (hTNF-alpha), matrix metalloproteinase 1 (MMP1), and foldon sequence using the methord of gene engineering. We transformed the recombinant plasmid, which contains the DNA sequences of hTNF-alpha, MMP1, and foldon sequence, into Rosetta2, and successfully induced the fusion protein to express under given conditions by isopropyl beta-D-1-Thiogalactopyranoside (IPTG). Then we purified the expression product through a glutathione S-transferase (GST) resin and collected the interested protein. This research may lay the groundwork for scientific research and clinical application.
Base Sequence
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Escherichia coli
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genetics
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metabolism
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Humans
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Matrix Metalloproteinase 1
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biosynthesis
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genetics
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Molecular Sequence Data
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Recombinant Fusion Proteins
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biosynthesis
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genetics
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Tumor Necrosis Factor-alpha
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biosynthesis
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genetics