1.Mechanisms of peripheral basophils activation in patients with rheumatoid ar-thritis
Qiaofen LAN ; Huanjin LIAO ; Qiuhua CHEN ; Jun CAI ; Lifang ZHANG ; Yanxia PENG ; Yiming HE ; Ping WU ; Tong XIE ; Qingjun PAN
Chinese Journal of Immunology 2017;33(1):85-89
Objective:To investigate the activation of peripheral basophils from patients with rheumatoid arthritis ( RA) and its mechanisms. Methods:The activation markers including CD203c and the proportion of IL-4 positive rate of peripheral basophils from RA patients and healthy controls were detected by flow cytometry. Serum levels of IgE in RA patients and healthy controls were detected by electrochemilu minescence immunoassay. Basophils were negatively isolated and co-cultured with or without purified IgE,anti-IgE as positive control,then,the expression of CD203c and propotion of IL-4 positive basophils were detected by flow cytometry. Results:The expression of CD203c and IL-4 positive rate of basophils from RA patients were higher than that of healthy controls (P<0. 05). Serum levels of IgE in RA were higher than that of healthy controls (P<0. 05). After co-cultured with isolated IgE from RA patients,basophils negatively isolated from healthy controls were activated,and higher expression of CD203c and proportion of IL-4 (P<0.05). Conclusion:Basophil activation is related with development of RA,and its activation is mainly mediated by IgE. Targeting basophil and its activation pathway would be expected to provide new strategies for the treatment of RA.
2.Identification and repositioning of catheter malposition during PICC insertion in 113 cancer patients
Lewen SHAO ; Xiaorong HU ; Aiyun JIN ; Qiaofen LIAO ; Weiming LU
Chinese Journal of Nursing 2018;53(4):454-456
This paper summarized identification and reposition strategies of catheter malposition in 113 cancer patients with peripherally inserted central catheters(PICCs) during catheterization.In the process of PICC catheterization,catheter malposition was identified by ultrasound and ECG in a real-time manner.A series of timely and effective reposition procedures were performed by internal jugular vein blocking,breathing combined with anterior segment wire withdrawal Overall,111 cases were successfully repositioned,the success rate was 98.23%,and 2 cases failed because of other reasons such as diseases.