1.Extraction of Texture Feature Parameter of Transverse Section in Chinese Herbal Medicine by Gray-Level Co-occurrence Matrix
Ou TAO ; Yanling ZHANG ; Qian CHEN ; Yun WANG ; Yanjiang QIAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(12):2531-2537
Digitalization is an important method for the objectification and quantification of quality control on Chi-nese herbal medicine. To solve the problem of texture online visualization of Chinese herbal medicine and the estab-lishment of automatic identification method based on the texture, 12 Chinese herbal medicines were selected to cap-ture the images of their transverse sections. A total of 11 features were extracted from images using Gray-level Co-occurrence Matrix (GLCM) method. Through analyzing the influence of distances and angles between pixels on identi-fication results, it was concluded that when the distance was d = 3 and the angle was ? = 0o, the features extracted were suitable for expressing the texture of the transverse sections. The results indicated the feasibility of establishing the automatic identification method on Chinese herbal medicine based on image of transverse section. It will provide new technologies for the objectification and quantification of identification on Chinese herbal medicine.
2.Isolation, Identification and 16S rDNA Sequences Analysis of a Bacterial Resistant to Copper and Cadmium
Yuan-Yuan PAN ; Wen-Li CHEN ; Qiao-Yun HUANG ;
Microbiology 1992;0(03):-
A strain of resistance to copper and cadmium with high concentration, named NTG-01, was isolated from soils of DaYe county mineral area in HuBei province. It can resist copper of 4.5mmol/L and cadmium of 2mmol/L , so we can say that it is a important strain used to study the resistance mechanism of copper and cadmium. A series of morphological and biochemical characteristics and sequences analysis of 16S rDNA reveal that it belongs to the bacteria and is gram negative, short rod, flagella around, the size of bacteria is about 0.8?m?2.0?m , V-P result shows positive, methyl-red result displays negative, and glucose can be utilized to produce acid and gas; In addition to, we find that it has the percent 99 homologous to Enterobacteraerogenes by 16S rDNA sequences BLAST analysis, plus the results of morphological and biochemical parameters, it belongs to Enterobacteraerogenes. We can conclude that NTG-01 has higher resistance to many different heavy metals by measuring MICs values of nine heavy metals at last.
3.Value of DWI for early assessment of response to concurrent chemoradiotherapy for esophageal cancer:a preliminary study
Wei CHEN ; Shengli ZHOU ; Chongchang MIAO ; Xiaodong JIANG ; Yun QIAO
Chinese Journal of Radiation Oncology 2014;23(4):312-316
Objective To investigate the feasibility and value of diffusion-weighted imaging (DWI) in the prediction and early assessment of response to concurrent chemoradiotherapy (CCRT) for esophageal cancer.Methods A total of 40 patients with pathologically confirmed esophageal cancer who received CCRT were included in the study.Routine 3.0 T MRI and DWI were performed at different time points of treatment.The RECIST standard was adopted to evaluate short-term outcomes and divide the patients into remission group (complete remission and partial remission) and non-remission group (stable disease and progressive disease).Group t-test was used for between-group comparison.The receiver operating characteristic (ROC) curve was used to analyze the change rates of apparent diffusion coefficient (ADC) value at different time points of treatment.Results There were 30 patients in the remission group and 10 patients in the non-remission group.The remission group had a significantly higher increase in ADC value than the non-remission group by the end of the first week of treatment (P =0.000).The maximum diameters of tumors for the emission group and non-remission group at the end of the first week of treatment were not significantly different from those before treatment (66.10 mm vs.62.63 mm,P =0.407 ; 70.90 mm vs.68.30 m,P =0.552).The ADC value before treatment had a negative correlation with the reduction rate of the maximum diameter of tumor (r =-0.680,P =0.000).The area under the ROC curve was the largest at the end of the first week of treatment (Az =0.783).If using 15.5 % increase in ADC value by the end of the first week as the threshold value for evaluating tumor response,the sensitivity,specificity,positive predictive value,and negative predictive value were 86.7%,70.0%,89.7%,and 63.6%,respectively.Conclusions DWI can be used as a new imaging method for the prediction and early assessment of the response to CCRT for esophageal cancer.The change rate of ADC value by the end of the first week of treatment is sensitive in assessing treatment response,so ADC value can be monitored at this time point.
4. Protective effects of aucubin on keratinocyte damaged by Ultraviolet B
Chinese Pharmaceutical Journal 2014;49(7):554-558
OBJECTIVE: To investigate the protection of aucubin on keratinocyte damaged by Ultraviolet B and explore its possible mechanism. METHODS: The photo damage model of keratinocyte was established by irradiating of UVB (64 mJ · cm-1). The different concentration aucubin were used for damaged cell. The cell viability was detected by MTT, the SOD activity, GSH-Px activity, CAT activity, MDA content were assayed by kit respectively. The mRNA levels of P38, TNF-α and IL-6 were determined by RT-PCR. The secretion levels of TNF-α and IL-6 of cultured keratinocyte were detected by ELISA. RESULTS: After the UVB irradiation, the cell viability, the activities of SOD, GSH-Px and CAT were decreased, the content of MDA, TNF-α and IL-6, the mRNA levels of P38, TNF-α and IL-6 were increased (P < 0.01). The above changes were inhibited by 1 × 10-1 and 1 × 10-1 mol · L-1 aucubin (P < 0.05 or P < 0.01). CONCLUSION: The aucubin could inhibit the injury by UVB for keratinocyte. And its possible mechanism may have relationship with the inhibition of oxidative damage, regulatory P38 signal pathway, adjusting the expression of TNF-α and IL-6.
5.Analysis of 7 cases of simple transumbilical single-tunnel laparoscopic renal cyst decortication
Yonghui LI ; Bagenbatu CHEN ; Jiankun QIAO ; La TA ; Jie LIU ; Zaohui TAN ; Zhizhong YUN
Chinese Journal of Primary Medicine and Pharmacy 2013;20(21):3204-3205,后插2
Objective To investigate the clinical applicated value of simple transumbilical single-tunnel laparoscopic renal cyst decortication.Methods 7 patients with renal cysts were treated with simple single-tunnel laparoscopic renal cyst decortication.Results The operations of 7 patients were succeed.The mean operative time was 37 (28 ~ 90) min,and the mean time of pulling the drain tube out was 2 (1 ~ 4) d,and the mean int aoperative blood loss was 30 (25 ~ 80) ml,and the mean bed stay was 1.5 (1 ~ 3) d,and the mean hospital stay was 4 (3 ~ 8) d.In 7 patients,no recurrence of the cyst occurred during a follow-up of 1 ~ 6 months by color-ultrasonography,CT after operation.Conclusion The simple transumbilical single-tunnel laparoscopic renal cyst decortication has advantages of economic,minimal trauma,hairdressing,rapid recovery and better effect,therefore it maybe an ideal treatment choice for renal cysts.
6.Application of Drupal in Chinese Medicine Information Fusion
Zhao CHEN ; Li MA ; Baixia ZHANG ; Bin YUAN ; Shuaibing HE ; Yun WANG ; Yanjiang QIAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(12):2563-2567
This paper was aimed to discuss the application of content management system (CMS) Drupal in Chinese medicine information fusion. The modular functionality, scalability and strong management capabilities of Drupal can effectively manage and reasonably present information resources of Chinese medicine, which extended the application of Chinese medicine information fusion. Regarding CMS Drupal as the carrier of Chinese medicine information fusion, the Chinese medicine information fusion method can be used in the collection, organization and demonstration of Chinese medicine information in the platform. As one of network tools for Chinese medicine information fusion, Dru-pal will play its powerful function in content management and information publishing with the virtual technology. It will also enrich Chinese medicine information fusion, and promote the spread of Chinese medicine knowledge.
7.Drug Resistance of Pathogens in Blood Culture Specimens in Shaanxi Antimicrobial Resistant Investigation Net in 2 0 1 5
Qiaodi GUI ; Jiankang REN ; Yanyan GONG ; Jinrong CANG ; Yun FAN ; Miao CHEN ; Jun QIAO
Journal of Modern Laboratory Medicine 2016;31(5):59-61,65
Objective To explore the bacterial drug resistance in blood culture in 2015 from the members of Antimicrobial Re-sistant Investigation Net of Shaanxi province,and to guide the clinicians touse antimicrobial drugs rationally.Methods All the objective bacterial isolates were collected and identifiedsusceptibility date by software WHONET 5.6.Results 6 871 bacterial isolates and their antibacterial susceptibilitydata were collected which included 3 199 (46.56%)Gram-negative bac-terial isolates and 3 672 (53.44%)Gram-positivebacterial isolates.The top five populationsof Gram-positive bacterial iso-lates were Staphylococcus epidermidis (30.94%),Staphylococcus hominis (17.84%),Staphylococcus haemolyticus (11.74%),Staphylococcusaureus (9.69%)and Enterococcus feacium (6.29%).The top five populationsof Gram-negative bacterial isolates were E.coli (43.67%),Stenotrophomonas maltophilia (14.63%),K.pneumoniae (13.47%), P.aeruginosa (4.13%)and Acinetobacter baumannii (3.63%).Theisolating rates of methicillin-resistant Staphylococcus aureus (MRSA)and methicillin-resistant coagulase negative Staphylococcus (MRCNS)were 31.2% and 76.1%,respec-tively.No vancomycin resistant Staphylococcusisolates were found.There were 0.9% E.faecium vancomycin-resistant iso-lates.The isolates of Enterobacteriaceae were still highly susceptible to carbapenem,whosetotal resistance rate was below 4.0%.The resistance rates of A.baumannii to most surveillance drugs in cludingimipenem were above 50.0% and the iso-lates of P.aeruginosa were still highly susceptible to most surveillancedrugs.Conclusion It is severe that the situation of bacterial drug resistance in blood culture in Shaanxi province.Should fullyuse bacterial drug resistance surveillance results for supervision and administration,and take effective measures forcontrolling the spread of resistant isolates.
8.Renal Hemodynamics in Patients with End Stage Renal Disease
Yun-Feng XIA ; Hua GAN ; Zheng-Rong LI ; En-Jing CHEN ; Lin QIAO ; Xiao-Ling HUANG ;
Chinese Journal of Hypertension 2007;0(03):-
Objective To investigate the relationship between refractory hypertension and renal hemodynamics in end stage renal diseases (ESRD) patients.Methods ESRD patients were classified into:patients with refractory hypertension (group A) and patients with normal blood pressure(group B).Renal hemodynamic indices were ex- amined by duplex ultrasonography.Fasting serum lipid (TC,TG,HDL-C,LDL-C,Lp(a),ox-LDL) and serum parathyroid hormane (PTH) were determined in all patients.Results Significant differences were found in renal hemodynamic indices such as peak systolic velocity (PSV),mean flow velocity (MV),pulsatility index (PI),renal- aortic ratio (RAR) and in clinical index such as Lp(a) and ox-LDL between the two group.Refractory hyperten- sion patients had lower renal hemodynamic indices and higher Lp(a) and ox-LDL levels than in patients with con- trolled BP.Logistic regression analysis revealed that refractory hypertension was related with PSV,EDV,Pl and RAR,but not relevant with sex,age,dialysis time,hematocrit,BUN,creatinine,TC,TG,HDL-C,LDL-C, PTH,MV and RI.Conclusion Atherosclerotic renal artery stenosis and severe disorder in renal hemodynamics is likely the cause for refractory hypertention in ESRD patients.The rise of serum Lp(a) and ox-LDL might acceler- ate renal artery atherosclerosis.
9.Effect of light quality on growth and quality of Pinellia ternata.
Yun CHEN ; Guo-Sheng ZHU ; Qiao-Sheng GUO ; Chang-Lin WANG ; Zuo-Yi LIU
China Journal of Chinese Materia Medica 2013;38(24):4257-4262
Measuring the content of soluble reducing sugar, total sugar, soluble protein, guanosine, alkaloids, and succinic acid of Pinellia ternata tuber were measured by anthrone-sulfuric acid colorimetric method, Coomassie brilliant blue method, RP-HPLC, reverse potentiometric titration, acid dye colorimetry, respectively. The result showed that yellow light could promote the growth and development of P. ternata and increase the content of soluble reducing sugar, total sugar, alkaloids, and succinic acid. Under blue light could promote the content of soluble protein and guanosine. Red and yellow light increased the content of chlorophyll a and chlorophyll b, contrastively blue light reduced the content of chlorophyll a and chlorophyll b. White film through the most uniform spectrum was most conducive to the synthesis of chlorophyll a. As single film, blue film, yellow film were more conducive to the synthesis of chlorophyll a, green film and red film had been relatively beneficial to the synthesis of chlorophyll b. Bulbil formed the largest number and the biggest propagation coefficient of P. ternata under red light showed that it could increase the production of P. ternata under red light.
Carbohydrate Metabolism
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radiation effects
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Chlorophyll
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metabolism
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Light
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Pinellia
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growth & development
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metabolism
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radiation effects
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Plant Leaves
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growth & development
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radiation effects
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Plant Proteins
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chemistry
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metabolism
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Quality Control
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Solubility
10.Expression changes of intermedin and calcitonin receptor-like receptor in the kidney of rats after renal ischemia reperfusion injury
Guihua YU ; Rongshan LI ; Xi QIAO ; Yun ZHOU ; Min KOU ; Chen WANG ; Bo BAI ; Shan SHAO
Chinese Journal of Nephrology 2009;25(5):387-391
Objective To investigate the expressions of intermedin /adrenomeduliin 2 (IMD/AM2) and its receptor calcitonin receptor-like receptor (CRLR) in the kidney of rats after renal ischemia reperfusion injury(IRI). Methods Male Wistar rats were divided randomly into two groups: sham group and operation group. Renal IRI model was induced by clamping both renal arteries. Blood and kidney were harversted at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h after reperfusion, respectively. Renal histological changes were semi-quantitated. Expressions of IMD and CRLR in the kidney were detected by Western blot, and the content of IMD in serum was measured by radioimmunity at 12 h, 48 h, 72 h after repeffusion. Results Kidneys of renal IRI model rats displayed significant pathologic changes, and the changes were much severer at 48 h after reperfusion. The expressions of IMD and CRLR in kidney were significantly up-regnlated at 12 h, 48 h, 72 h after renal IRI (P<0.01). The level of IMD in serum increased at 12 h, 48 h, 72 h after renal IRI (P<0.05). Conclusion The expressions of IMD and its receptor are up-regulated in the kidney after renal IRI, which may participate in the pathophysiological changes induced by renal IRI.