1.Chronic invasive fungal rhinosinusitis complicated with infratemporal fossa fungal infection: 2 cases report.
Wen LI ; Xiaoming QIAO ; Liu YANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(4):276-279
Two cases of chronic invasive fungal rhinosinusitis were reported. One patient healed while another died with suspicious residual fungal infection. The clinical symptoms of infratemporal fungal infection include maxillofacial pain, with or without fever. Acute or chronic inflammation of soft tissue could be revealed pathologically. Proof of pathogen may not be found in histopathological slice while serological detection may reveal positive evidence. Surgical resection, good drainage and postoperative antifungal therapy could cure or promptly relieve the symptoms of the disease.
Aged
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Chronic Disease
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Female
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Fungi
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Humans
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Male
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Middle Aged
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Mycoses
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Sinusitis
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microbiology
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Skull Base
2.Application of Neural Network in Forecasting Emergencies Caused by Meteorological Factors
Chinese Journal of Medical Physics 2010;27(2):1806-1809,1820
Objective: By analyzing the relationship between the meteorological factors and the outbreak of emergencies, an artificial neutral network model was established in order to make the medical-meteorological forecast and to reduce and prevent the emergencies. Methods: The data of emergency cases and meteorological factors within the same time in 2007 and 2008 in Ji'nan, China were collected and analyzed by using SAS9.0. The back-propagation (BP) artificial neutral network model was built by using Matlab7.0. Results: A close relationship exists between the meteorological factors as well as their changes and emergencies. The results of forecast show high accuracy rate of each emergency (76% ~ 89%) except CO poisoning (46%). Conclusions: This emergencies medical-meteorological forecasting model based on BP neutral network has good prediction effect and the value of further research.
3.Intermedin ameliorates renal injury by inhibition of tubular epithelial cell apoptosis in a renal ischemia/reperfusion rat model
Xinyan LIU ; Xinghua LIU ; Xi QIAO ; Hong LI ; Rongshan LI
Chinese Journal of Nephrology 2011;27(1):29-33
Objective To investigate the effect of intermedin (IMD) on tubular cells apoptosis induced by renal ischemia/reperfusion (I/R) injury and its associated mechanism.Methods A total of twenty-four male Wistar rats were randomly divided into four groups (control group, I/R group, empty plasmid group and IMD group). One week after the removal of right kidney, ultrasound plasmid was used to transfect empty or IMD plasmid into the left kidney. Renal I/R model was made by clasping the left renal artery for 45 minutes. Tubular cell apoptosis was determined by TUNEL. Expression of Bax, Bcl-2, Fas was detected by semi-quantitative RT-PCR.Activity of caspase-8 and caspase-9 was evaluated with commercially available kits respectively.Protein level of caspase-3 was measured by Western blotting analysis. Results Compared with control group, apoptosis of tubular epithelial cells, expression of Bax and Fas, activities of caspase-8 and caspase-9, as well as protein level of caspase-3 were all significantly increased in I/R group (all P<0.05). IMD pre-treatment significantly inhibited all these effects (all P<0.05). There were no differences of above parameters between empty plasmid group and I/R group. Conclusion IMD pre-treatment protects against renal I/R injury by inhibion of tubular epithelial cell apoptosis.
4.Clinical characteristics and prognosis of community-acquired and hospital-acquired acute kidney injury
Mingming QIAO ; Shun WANG ; Caichang LI ; Jian LIU ; Suhua LI
Chinese Journal of Nephrology 2016;32(1):16-23
Objective To compare the clinical characteristics of community-acquired acute kidney injury (CA-AKI) and hospital-acquired acute kidney injury (HA-AKI) patients.Methods Hospital network system was employed to screen the clinical data of adult patients in the First Affiliated Hospital in Xinjiang Medical University in January to July 2013.A total of 19 528 patients were screened,and 544 AKI patients were identified based on KIDGO (Kidney Disease:Improving Global Outcomes) AKI guidelines.Three hundred and thirty patients were included in HA-AKI group and 214 patients in CA-AKI group.Clinical variables including mortality were analyzed retrospectively.Results The incidence of AKI in hospitalized patients was 2.8% (544/19 528):1.7% in CA-AKI group and 1.1% in HA-AKI group.The mean age in CA-AKI group was significantly older than that in HA-AKI group [(62.9 ± 16.8) years vs (56.6± 15.9) years].Medical patients in CA-AKI group accounted for 62.4%,and surgical patients in HA-AKI group accounted for 64.1%.The co-morbid diseases were cardiac disease,hypertension,diabetes and chronic liver disease.Majority of AKI was caused by pre-renal etiologies.The length of hospitalization was significantly shorter in CA-AKI group compared to that in HA-AKI group [12(8,20) days vs 19 (12,27) days,P < 0.01].Compared to that in HA-AKI group,all-cause mortality was significantly lower in CA-AKI group (11.5% vs 20.1%,P=0.005).Results by multivariate logistic regression analysis demonstrated that the common independent risk factors of AKI in both groups were ICU hospitalization and shock.The independent risk factor of AKI in CA-AKI group was diabetes (OR=3.019).In contrary,the independent risk factors of AKI in HA-AKI group were elderly (≥65 years) (OR=3.303),oliguria (24 h urine volume < 400 ml) (OR=6.906),use of antiinflammatory drugs (OR=13.079) and multiple organ dysfunction syndrome (OR=17.778).Conclusions The incidence of AKI in hospitalized patients is not rare,among which both communityacquired and hospital-acquired AKI are mainly caused by pre-renal etiologies.All-cause mortality is lower in community-acquired AKI compared to that in hospital-acquired AKI and the independent risk factors are different between CA-AKI and HA-AKI.
5.Effect of allo-human bone marrow mesenchymal stem cells on T and B cells from patients with rheumatoid arthritis in vitro
Lihui MA ; Xiaofeng LI ; Zhenhua QIAO ; Zhizhen LIU ; Fang LI
Chinese Journal of Rheumatology 2009;13(6):386-389
Objective To study the effect of allo-human bone marrow mesenchymal stem cells (BMSCs) on T and B cells from patients with Rheumatoid arthritis (RA) in vitro. Methods BMSCs were isolated from bone marrow samples of healthy volunteers and purified by density gradient centrifugation and cultured in vitro. Peripheral lymphocytes were isolated from patients with RA.Then, BMSCs and lymphpcutes were co-cultured. The modulatory effect of BMSCs on proliferation, activation and maturation of T and B lymphocytes of RA patients stimulated by PHA and SAC respectively was observed. The cell generation cycle and the degree of apoptosis were assessed by flow cytometry with PI/ Annexin V. After co-cultured with or without BMSCs for 72 hours, T cells were harvested, then they were labeled with anti-CD3, anti-CD4, anti-CD8, anti-CD25 antibodies and analyzed by flow cytometry. The density of IgG in the co-culture system was detected by ELISA. Results T and B cells proliferation was significantly suppressed when co-cuhured with bMSCs but did not induce T cell apoptosis. There was a significant decrease in the ratio of CD4+ CD3+ T cells in the co-cuhure group (34±6), as compared with the control group (44±7) (P<0.05). There was a decrease in CD25+ T cells and increase of CD4+ CD25+ cells in BMSCs co-cultured group (P<0.05). IgG was in creased in the cocuhure system. Conclusion Human BMSCs inhibit T and B cell activation and proliferation in patients with RA in vitro. And these immunomodulatory effects are not MHC restricted. The results of this study have provided evidence for the fact that BMSCs has the potential to be an effective treatment for RA.
7.The application of developmental assessment in the core specialty courses of emergency and inten-sive care orientation in nursing profession
Yongyun TANG ; Chuanxia ZHANG ; Linlin LI ; Changxiu QIAO ; Chengxia LIU
Chinese Journal of Medical Education Research 2016;15(2):149-153
To construct “developmental assessment” in the course of emergency nursing and inten-sive care nursing by applying the combination of formative assessment and summative evaluation, the com-bination of qualitative assessment and quantitative assessment and the incorporation of self-assessment and other evaluation. Specifically, this project contains investigation activity (accounting for 10%), operating activity (10%), checking on work attendance and homework evaluation (15%), reflection activity (15%), comprehensive design experiment evaluation (20%) and theoretical evaluation (30%). Process evaluation and self-evaluation are highlighted, especially feedback and improvement after evaluation. Through multi stage and multi form evaluation, the goal of emergency and critical care professional education has been achieved, which has promoted the development of student nurses' quality and overall development.
8.Study on the Relationship Between Genotypes and Phenotypes of Trichophyton rubrum
Guoling YANG ; Qiao LI ; Xiaohong YU ; Weida LIU ; Xiran LIN
Chinese Journal of Dermatology 2003;0(12):-
Objective To study the relationship between genotypes and phenotypes,geographical distribution,and the sites of infection of Trichophyton rubrum(T.rubrum).Methods The genotypes were determined by Southern blotting with a probe amplified from the small-subunit rDNA and adjacent internal transcribed spacer(ITS)regions.The phenotypes of T.rubrum were determined by conventional method.Results Twenty genotypes(DNA type A to T)and5phenotypes(villous,furrowed,granular,powdery,and woolly)were recognized among49strains of T.rubrum.Genotype A prevailed in all phenotypes except granu-lar type.Type B represented the most common genotype among the strains of villous type and furrowed type.Type A took the first place in powdery type and woolly type.All of the type A strains were from Dalian.Seven of9type B strains were from Nanjing.Six type C strains were all from Nanjing.The majority strains of21strains isolated from tinea unguium were type C,most of the16strains isolated from tinea cruris and tinea corporis were type A,8strains from tinea pedis were type B,and4strains from tinea capitis were type C.Conclusion There are certain possible relationships between genotypes and phenotypes,geographical distri-bution and sites of infection of T.rubrum.
9.Agrobacterium tumefaciens-mediated transformation of uracil auxotroph Aspergillus fumigatus is an efficient method for target gene knockout
Jianjun QIAO ; Wei LIU ; Yan MA ; Zhe WAN ; Ruoyu LI
Journal of Peking University(Health Sciences) 2004;0(03):-
Objective:To investigate the efficiency of Agrobacterium tumefaciensmediated transfor-mation of Aspergillus fumigatus by using pyrG as a recessive selectable marker.Methods: FAP1 and SHO1 genes target sequences,composed of a selectable marker pyrG and the flanking sequences of the FAP1 and the SHO1 genes,were cloned into a binary plasmid pDHt/sk,respectively.The produced plasmids were transformed into A.tumefaciens.The A.tumefaciens and uracil auxotroph A.fumigatus were cocultured in induction medium without uricil and uridine at 24 ℃ for 48 h.To inhibit growth of A.tumefaciens and to select transformants,the cultures were transferred to 37 ℃ and incubated for another 48 h.Results: In this study,A.tumefaciens-medidated transformation of A.fumigatus produced high homologous recombination rates,which was 44%(7 of 16) for FAP1 and 35%(7 of 20) for SHO1.Conclusion: Our study showed that A.tumefaciens-medidated transformation by using pyrG as a recessive selectable marker is an efficient tool for target gene deletion of A.fumigatus.
10.Detection of human parvovirus B19 nonstrutural protein DNA by nested-polymerase chain reaction in gravida serum and pregnant tissues.
Ting, SHEN ; Yongmei, HUANG ; Fuyuan, QIAO ; Zengqing, LI ; Haiyi, LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2006;26(1):123-6
A new nested-polymerase chain reaction (nested-PCR) assay was developed to detect human parvovirus B19 DNA corresponding to the nonstructural protein in clinical specimens in a routine diagnostic laboratory. The sensitivity of this highly specific assay was up to 0.005 fg of B19 DNA. Parvovirus B19 was identified in sera of 20 pregnant women with abnormal pregnant outcome. Among these 20 cases, intrauterine parvovirus infection did exist in 7 pregnant women because parvovirus B19 DNA was detected in the pregnant tissues of them such as placenta tissues, chorionic villi, amniotic fluid, fetal spleen, liver and abdominal fluids.
DNA, Viral/*analysis
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Parvoviridae Infections/*virology
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Parvovirus B19, Human/genetics
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Parvovirus B19, Human/*isolation & purification
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Placenta/virology
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Pregnancy Complications, Infectious/diagnosis
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Pregnancy Complications, Infectious/*virology
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Viral Nonstructural Proteins/*analysis