Object To assess the genetic diversity among ten natural populations in Ophiopogon japonicus (L f ) Ker Gawl and O. bodinieri Levl (Liliaceae) in Sichuan Province Methods Forty random decamer primers were screened for Random Amplified Polymophic DNA (RAPD) fragments Results Based on cluster analysis of 515 DNA bands amplified by 11 primers, a DNA molecular dendrogram was established, and the relationship of the populations and seeds between O japonicus and O bodinieri was set up Conclusion 1 Distinct genetic differences and extensive genetic diversity are presented among the samples The genetic differences are related to morphological differences, but not to geographic regions; 2 Quite genetic differences are presented between O japonicus and O bodinieri, it suggests that they have farther relationships

Adult ; Enzyme-Linked Immunosorbent Assay ; Female ; HSP70 Heat-Shock Proteins ; blood ; Humans ; Lead ; toxicity ; Male ; Occupational Exposure

Objective To investigate the mechanism of therapeutic action of dexamethasone on asthmatic mice by detecting the levels of IL-25 and IFN-γ in bronchoalveolar lavage fluid (BALF). Methods Balb/c mice with SPF grade were randomly divided into normal control group, asthma group and dexamethasone group. Asthma group and dexamethasone group were sensitized and challenged with ovalbumin ( OVA) . Dexamethasone group was intraperitoneally injected with dexamethasone one hour before challenging. The mice were executed 24 hours after the last challenge, and the HE stained pathological sections of the right lung were made. Pathological sections of lung were observed. BALF in the left lung was also collected. The total white blood cell count and absolute eosinophile ( EOS) count were observed, and the percentage of EOS was calculated. The levels of IL-25 and IFN-γwere measured with ELISA, and correlation analyses were made. Results The counts of total white blood cell and EOS, and the percentage of EOS were significantly higher in the asthma group than in the normal control group and dexamethasone group (P<0. 05). No differences were found between the normal control group and dexamethasone group. The IL-25 level was higher in the asthma group than in the normal control group and dexamethasone group (P<0. 05), and its level in the dexamethasone group was also higher than that in the normal control group. The IFN-γlevel was lower in the asthma group than in the normal control group and dexamethasone group (P<0. 05), while there was no significant difference between the normal control group and dexamethasone group. IL-25 was negatively correlated with IFN-γin each group. Conclusion Part of the mechanisms of dexamethasone acting on asthma are related to its inhibition on the pulmonary inflammation and promotion on the expression of IFN-γ, and possible inhibition of IL-25 expression.

Humans ; Lithiasis ; surgery ; Liver Diseases ; surgery ; Liver Transplantation ; Male ; Middle Aged ; Situs Inversus ; complications ; surgery

OBJECTIVETo measure the thickness of facial bone wall of maxillary anterior teeth and premolars based on cone beam computed tomography (CBCT) images.

METHODSCBCT images from 118 patients were collected from the Affiliated Stomatology Hospital of Zhejiang University. The thickness perpendicular to the long axis of facial bone wall was measured at two locations: 4 mm apical to the cementoenamel junction (point 1) and the middle of the root (point 2).

RESULTSThe thickness of the facial bone walls of central incisors, lateral incisors and canines ranged from 0.5 to 1.5 mm. A thin facial bone wall (<1.0 mm) was quite frequent in central incisors (44.1% at point 1, 56.8% at point 2), lateral incisors (65.2% at point 1, 89.8% at point 2) and canines (45.8% at point 1, 61.0% at point 2). In contrast, the majority of examined first premolars (77.1% at point 1, 68.7% at point 2) and second premolars (94.1% at point 1, 94.1% at point 2) exhibited a thick facial bone wall (>1.0 mm).

CONCLUSIONA thin facial bone wall of teeth in the anterior maxilla is common. Radiographic analysis of facial bone wall using CBCT is recommended for selection of appropriate treatment approach.

Adult ; Bicuspid ; diagnostic imaging ; Cone-Beam Computed Tomography ; Female ; Humans ; Incisor ; diagnostic imaging ; Male ; Maxilla ; diagnostic imaging ; Young Adult

Coronary heart disease (CHD) related genes and targets, as well as drug targets for preventing and treating CHD were taken as the study objects to build a CHD disease network and a drug action network preventing and treating CHD. Such topological characteristic parameters of the networks as degree distribution, characteristic path length, connectivity and heterogeneity were analyzed to verify the reliability of the networks. On that basis, the intersection calculation was conducted for both networks to analyze the drug action mechanism of their sub-networks. The disease network are composed of 15,221 nodes and 31,177 sides, while the drug action network preventing and treating CHD has 15,073 nodes and 32,376 sides. Both of their topological characteristic parameters showed scale-free small world structural characteristics. Two reaction pathways in the sub-networks-calcitonin gene-related peptide and IL-6 activated JAK/STAT were taken as examples to discuss the indirect action mechanism for preventing and treating CHD. The results showed that the biological network analysis method combining the disease network and the drug action network is helpful to further studies on the action mechanism of the drugs, and significant to the prevention and treatment of diseases.
Computational Biology ; Coronary Artery Disease ; drug therapy ; genetics ; pathology ; prevention & control ; Databases, Genetic ; Humans ; Molecular Targeted Therapy ; Signal Transduction ; drug effects

To analyze the intersections among the western medicine action network for preventing and treating coronary heart disease (CHD), as well as traditional Chinese medicine (TCM) action network for activating blood and dissolving stasis. In this article, 11 characteristic parameter values of network nodes, including connectivity, bottleneck, betweenness, were calculated. The target identification model was established based on key node characteristic parameters in the CHD-western medicine intersection network with support vector machine. Its C and y parameters were 5.14 and--1.11, respectively, with the predicted accuracies for positive and negative samples of 81.6% and 79.2%. The predicted sensitivity, specificity and accuracy of the test set samples were 81.5%, 78.3% and 79.6%, respectively. Besides, the model was applied to predict potential action targets of the CHD-activating blood and dissolving stasis TCM intersection network, and 180 positive nodes and 42 negative nodes were obtained. In this article, 9 positive nodes, including calnexin, interleukin-1, tumor necrosis factor, were taken as examples to analyze the action mechanism of TCM for preventing and treating CHD. The results showed that the network potential key target analysis method was helpful to explore the potential action targets of activating blood and dissolving stasis TCMs for preventing and treating CHD, methodologically supportive to reveal the action mechanism of TCMs at molecular and systematic levels, and significant in guiding the research and development of new drugs.
Coronary Artery Disease ; drug therapy ; metabolism ; prevention & control ; therapy ; Drug Delivery Systems ; Drugs, Chinese Herbal ; Humans ; Medicine, Chinese Traditional ; methods ; Signal Transduction

AIM:To observe the concentration of RBP4 and IL-6 in vitreous of proliferative diabetic retinopathy (PDR). METHODS: A total of 65 patients (66 eyes) were enrolled in Department of Ophthalmology, Renmin Hospital of Wuhan University from February 2017 to July 2017 with the informed consent. The patients were divided into PDR group (23 cases) and NPDR group (16 cases). Twenty- six patients without diabetic mellitus (DM) served as control group. The demography was matched among the groups, but the course of DM, the blood glucose level and the HbA1c level were elevated in the PDR group and the NPDR group (all P<0.05). Vitreous samples were collected during the procedure of vitrectomy. RBP4,IL-6,TNF-α concentrations in vitreous specimens were detected by ELISA. The differences of vitreous RBP4, IL-6 and TNF-α in various groups were statistically analyzed by ANOVA, respectively. The correlations between RBP4 and IL - 6, TNF - α were calculated by Pearson correlation analysis. RESULTS:The concentration of RBP4 in PDR group,the NPDR group and control group were 13.68士2.66, 11 03士1 12,10.45士1.17μ g/Ml, and the concentration of IL-6 were 56.0士10.27, 20.92士5.77, 10.26士1.91pg/Ml. RBP4 and IL- 6 concentrations were elevated in PDR group compared with NPDR group and control group, with significant difference among three groups (F = 12. 135, 161.167; P < 0. 01). IL - 6 concentrations in vitreous increased in the NPDR group in comparison with control group(P<0.05). RBP4 concentrations had no significant difference between the NPDR group and the group(P>0 05). Pearson correlation coefficient was significant positive between RBP4 concentration and IL - 6 concentration(r=0.606,P=0.001). CONCLUSION: RBP4 is probability involved in the inflammation pathogenesis of PDR. These results indicate that RBP4 could be a new target for the diagnosis and treatment of PDR.

Bone Plates ; Fracture Fixation, Internal ; Humans ; Radius Fractures/surgery*

Objective To investigate the need for health knowledge of hemodialysis patients of different dialysis ages,in order to provide basis for health education.Methods Self-designed questionnaire was used to look into the need for health knowledge of 100 maintenance hemodialysis patients of different dialysis ages.Results Patients of different dialysis ages had different acknowledge of the understanding of health education,the necessity of health education,the influence of health education on life quality.The differences were statistically significant ( F =28.149,18.739,24.407,respectively ; P ＜ 0.001).Patients who had received dialysis less than 1year paid more attention to the effect of hemodialysis,the maintenance of vascular access and instantaneous complications.Patients who had received dialysis more than 1year but less than 5 years laid emphasis on the sufficiency of hemodialysis,drug use during hemodialysis,examination index and long-dated complications.Patients who had received dialysis more than 5 years wanted more knowledge on the long-dated complications of hemodialysis,new developments of dialysis treatment and life quality improvement,etc.Patients of different cultural levels had different requirements for health education modes.Explanation from doctors and nurses,videos were first choice of most patients,but education modes such as experience introduction from wardmate,reading related books,publicity column,lectures and internet search were differently welcomed between patients of different cultural levels,and the differences were statistically significant (F=14.950,6.020,11.772,6.744,13.057,respectively;P＜0.05).Conclusions As the main enforcers of health education,paramedics need to further popularize the concept of health education,and pay attention to the diversity of education mode according to the needs of patients of different dialysis ages,in order to fulfill the health education work.